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The map label for this gene is gspJ [H]

Identifier: 170679969

GI number: 170679969

Start: 3313392

End: 3313997

Strand: Reverse

Name: gspJ [H]

Synonym: EcSMS35_3241

Alternate gene names: 170679969

Gene position: 3313997-3313392 (Counterclockwise)

Preceding gene: 170679735

Following gene: 170684190

Centisome position: 65.39

GC content: 56.27

Gene sequence:

>606_bases
GTGAGAAGGGCTCGCGCTGGTTTCACGTTACTGGAAATGCTGGTGGCGATAGCCATTTTTGCCTCACTGGCACTGATGGC
GCAGCAGGTGACAAACGGCGTTACTCGCGTGAATAGCGCCGTCGCCGGACACGATCAAAAACTAAACCTCATGCAGCAAA
CGATGAGTTTTTTGAACCACGATCTGACACAAATGATGCCGCGTCCGGTAAGAGGCGATCAGGGCCAGCGTGAACCAGCG
TTACTGGCGGGCGCTGGCGTGCTGGCGTCTGAGAGTGAAGGGATGCGTTTTGTACGTGGTGGCGTGGTTAATCCGTTGAT
GCGTCTGCCGCGCAGTAATCTGCTCACCGTCGGTTACCGCATTCATGGTGGTTATCTCGAACGGTTAGCCTGGCCACTGA
CCGATGCGGCAGGCAGCGTGAAGCCAACAACGCAAAAATTAATCCCGGCGGATTCGCTCCGTTTGCAGTTCCACGACGGC
ACGCGCTGGCAGGAGAGCTGGTCATCAGTGCAGGCGATTCCTGTGGCAGTACGCATAACCCTGCATTCGCCGCAATGGGG
CGAAATTGAACGCATCTGGTTGTTACGCGGGCCGCAATTATCATGA

Upstream 100 bases:

>100_bases
TGCTGCTCCAGGCGGTGGATATTGAAGTCAGTCTTCATGACGACTTTTCGCCAGTGATTCAGTCAAGACGCGCCTGGTTT
AGCGCCGTGGGAGGCCAACA

Downstream 100 bases:

>100_bases
TGATCACCTTGCCACCAAAACGCGGAATGGCACTGGTCGTGGTGCTGGTATTGCTGGCGGTTATGATGCTGGTGACCATC
ACGCTTTCCGGGCGGATGCA

Product: general secretion pathway protein GspJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPA
LLAGAGVLASESEGMRFVRGGVVNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFHDG
TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS

Sequences:

>Translated_201_residues
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPA
LLAGAGVLASESEGMRFVRGGVVNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFHDG
TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS
>Mature_201_residues
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPA
LLAGAGVLASESEGMRFVRGGVVNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFHDG
TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 22243; Mature: 22243

Theoretical pI: Translated: 11.49; Mature: 11.49

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLNH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGVVNPLMRLPRSNLLTVGYR
HHHHHCCCCCCCCCCCCCCCEEECCCCEECCCCCCHHHHCCHHHHHHHCCCCCEEEEEEE
IHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFHDGTRWQESWSSVQAIPVAVRIT
ECCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHEEEEEEEEEE
LHSPQWGEIERIWLLRGPQLS
ECCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGHDQKLNLMQQTMSFLNH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGVVNPLMRLPRSNLLTVGYR
HHHHHCCCCCCCCCCCCCCCEEECCCCEECCCCCCHHHHCCHHHHHHHCCCCCEEEEEEE
IHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFHDGTRWQESWSSVQAIPVAVRIT
ECCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHEEEEEEEEEE
LHSPQWGEIERIWLLRGPQLS
ECCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407845 [H]