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The map label for this gene is gspK [H]

Identifier: 170684190

GI number: 170684190

Start: 3312415

End: 3313392

Strand: Reverse

Name: gspK [H]

Synonym: EcSMS35_3240

Alternate gene names: 170684190

Gene position: 3313392-3312415 (Counterclockwise)

Preceding gene: 170679969

Following gene: 170683391

Centisome position: 65.37

GC content: 55.52

Gene sequence:

>978_bases
ATGATCACCTTGCCACCAAAACGCGGAATGGCACTGGTCGTGGTGCTGGTATTGCTGGCGGTTATGATGCTGGTGACCAT
CACGCTTTCCGGGCGGATGCAGCAACAACTTGGGCGAACGCGCAGCCAGCAGGAGTACCAGCAGGCGCTGTGGTACAGCG
CCAGTGCAGAAAGCCTGGCGCTGAGCGCGCTCAGTCTGAGCCTGAAAAATGAAAAGCGTGTGCATCTGGCACAACCGTGG
GCTTCTGGCCCGCGTTTTTTCCCACTGCCGCAGGGGCAAATTGCCGTCACTCTGCGTGACGCACAGGCCTGCTTTAACCT
GAATGCCCTCGCTCAGCCGACGACGACGTCGCGTCCGCTCGCGGTACAACAACTGATTGCCCTGATCTCGCGCCTCGATG
TGCCTGCTTATCGGGCCGAACTGATAGCCGAAAGCCTGTGGGAGTTTATTGACGAAGACCGCAGTGTGCAGACGCGTCTG
GGTCGTGAAGACAGCGAGTATCTCGCCCGCTCGGTGCCGTTCTACGCCGCTAATCAACCGCTGGCTGATATCAGCGAGAT
GCGCGTGGTGCAGGGAATGGACGCTGGGCTTTATCAAAAACTGAAACCGCTGGTCTGTGCGCTGCCGATGGCCCGCCAGC
AAATCAACATCAATACATTAGATGTCACGCAAAGTGTGATTCTTGAGGCGCTGTTTGACCCGTGGTTAAGCCCTGTTCAG
GCGCGGGCGTTATTACAACAACGTCCGGCGAAGGGCTGGGAAGATGTCGATCAGTTTCTTGCTCAGCCGCTACTTGCAGA
CGTCGATGAGCGTACTAAAAAACAGCTAAAAACCATCCTGAGCGTGGACAGCAATTACTTCTGGCTGCGTTCAGATATCA
CCGTGAATGAGATTGAACTGACGATGAATTCGTTAATTGTCCGCATGGGCCCACAACACTTTTCTGTTCTCTGGCATCAG
ACAGGAGAAAGTGAGTGA

Upstream 100 bases:

>100_bases
CAGTGCAGGCGATTCCTGTGGCAGTACGCATAACCCTGCATTCGCCGCAATGGGGCGAAATTGAACGCATCTGGTTGTTA
CGCGGGCCGCAATTATCATG

Downstream 100 bases:

>100_bases
GTTCCATGCTTGAGATTTATTTCCCGCTTTGCGCCGCTGATCCCATCCGTTGGCAGCGCCGTACACCCGACGTGGAGCAC
GGCATCTGGCCTGACGTCGC

Product: general secretion pathway protein GspK

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTTSRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Sequences:

>Translated_325_residues
MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTTSRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE
>Mature_325_residues
MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTTSRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=287, Percent_Identity=34.8432055749129, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 36569; Mature: 36569

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTTSRPL
HHHHHHHHCCCCCEEEECCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTTSRPL
HHHHHHHHCCCCCEEEECCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]