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The map label for this gene is neuD [H]

Identifier: 170679748

GI number: 170679748

Start: 3305661

End: 3306284

Strand: Reverse

Name: neuD [H]

Synonym: EcSMS35_3233

Alternate gene names: 170679748

Gene position: 3306284-3305661 (Counterclockwise)

Preceding gene: 170680342

Following gene: 170684121

Centisome position: 65.23

GC content: 31.09

Gene sequence:

>624_bases
ATGAGTAAAAAATTAATAATATTTGGTGCGGGTGGTTTTTCAAAATCTATAATTGACAGCTTAAATCATAAACATTACGA
GTTAATAGGATTTATCGATAAATATAAAAGTGGTTATCATCAATCATATCCAATATTAGGTAATGATATTGCAGACATCG
AGAATAAGGATAATTATTATTATTTTATTGGGATAGGCAAACCATCAACTAGGAAGCACTATTTAAACATCATAAGAAAA
CATAATCTACGCTTAATTAACATTATAGATAAAACTGCTATTCTATCACCAAATATTATACTGGGTGATGGAATTTTTAT
TGGTAAAATGTGTATACTTAACCGTGATACTAGAATACATGATGCCGTTGTAATAAATACTAGGAGTTTAATTGAACATG
GTAATGAAATAGGCTGCTGTAGCAATATCTCTACTAATGTTGTACTTAATGGTGATGTTTCTGTTGGAGAAGAAACTTTT
GTTGGTAGCTGTACTGTTGTAAATGGCCAGTTGAAGCTAGGCTCAAAGAGTATTATTGGTTCTGGGTCGGTTGTAATTAG
AAATATACCAAGTAATGTTGTAGTTGCTGGGACTCCAACAAGATTAATTAGGGGGAATGAATGA

Upstream 100 bases:

>100_bases
TTTTATAAAAATGTTACGGAAGCCATTGCTGATTACAAAAAAGACCTATAGTGGTTACATTCCAATATTATGCCTTGGAA
ATATTTAACTGAGACATATC

Downstream 100 bases:

>100_bases
GTAATATATATATCGTTGCTGAAATTGGTTGCAACCATAATGGTAGTGTTGATATTGCAAGAGAAATGATATTAAAAGCC
AAAGAGGCCGGTGTTAATGC

Product: polysialic acid capsule biosynthesis protein NeuD

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSKKLIIFGAGGFSKSIIDSLNHKHYELIGFIDKYKSGYHQSYPILGNDIADIENKDNYYYFIGIGKPSTRKHYLNIIRK
HNLRLINIIDKTAILSPNIILGDGIFIGKMCILNRDTRIHDAVVINTRSLIEHGNEIGCCSNISTNVVLNGDVSVGEETF
VGSCTVVNGQLKLGSKSIIGSGSVVIRNIPSNVVVAGTPTRLIRGNE

Sequences:

>Translated_207_residues
MSKKLIIFGAGGFSKSIIDSLNHKHYELIGFIDKYKSGYHQSYPILGNDIADIENKDNYYYFIGIGKPSTRKHYLNIIRK
HNLRLINIIDKTAILSPNIILGDGIFIGKMCILNRDTRIHDAVVINTRSLIEHGNEIGCCSNISTNVVLNGDVSVGEETF
VGSCTVVNGQLKLGSKSIIGSGSVVIRNIPSNVVVAGTPTRLIRGNE
>Mature_206_residues
SKKLIIFGAGGFSKSIIDSLNHKHYELIGFIDKYKSGYHQSYPILGNDIADIENKDNYYYFIGIGKPSTRKHYLNIIRKH
NLRLINIIDKTAILSPNIILGDGIFIGKMCILNRDTRIHDAVVINTRSLIEHGNEIGCCSNISTNVVLNGDVSVGEETFV
GSCTVVNGQLKLGSKSIIGSGSVVIRNIPSNVVVAGTPTRLIRGNE

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22687; Mature: 22555

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLIIFGAGGFSKSIIDSLNHKHYELIGFIDKYKSGYHQSYPILGNDIADIENKDNYY
CCCEEEEEECCCCCHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
YFIGIGKPSTRKHYLNIIRKHNLRLINIIDKTAILSPNIILGDGIFIGKMCILNRDTRIH
EEEEECCCCCHHHHHHHHHHCCEEEEEEECCCEEECCCEEEECCEEEEEEEEECCCCCEE
DAVVINTRSLIEHGNEIGCCSNISTNVVLNGDVSVGEETFVGSCTVVNGQLKLGSKSIIG
EEEEEECHHHHHCCCCEEEECCCCCCEEEECCCCCCCCEEEEEEEEEECEEEECCEEEEC
SGSVVIRNIPSNVVVAGTPTRLIRGNE
CCCEEEEECCCCEEEECCCCEEEECCC
>Mature Secondary Structure 
SKKLIIFGAGGFSKSIIDSLNHKHYELIGFIDKYKSGYHQSYPILGNDIADIENKDNYY
CCEEEEEECCCCCHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
YFIGIGKPSTRKHYLNIIRKHNLRLINIIDKTAILSPNIILGDGIFIGKMCILNRDTRIH
EEEEECCCCCHHHHHHHHHHCCEEEEEEECCCEEECCCEEEECCEEEEEEEEECCCCCEE
DAVVINTRSLIEHGNEIGCCSNISTNVVLNGDVSVGEETFVGSCTVVNGQLKLGSKSIIG
EEEEEECHHHHHCCCCEEEECCCCCCEEEECCCCCCCCEEEEEEEEEECEEEECCEEEEC
SGSVVIRNIPSNVVVAGTPTRLIRGNE
CCCEEEEECCCCEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]