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The map label for this gene is neuB

Identifier: 170684121

GI number: 170684121

Start: 3304624

End: 3305664

Strand: Reverse

Name: neuB

Synonym: EcSMS35_3232

Alternate gene names: NA

Gene position: 3305664-3304624 (Counterclockwise)

Preceding gene: 170679748

Following gene: 170683470

Centisome position: 65.22

GC content: 32.37

Gene sequence:

>1041_bases
ATGAGTAATATATATATCGTTGCTGAAATTGGTTGCAACCATAATGGTAGTGTTGATATTGCAAGAGAAATGATATTAAA
AGCCAAAGAGGCCGGTGTTAATGCAGTAAAATTCCAAACATTTAAAGCTGATAAATTAATTTCAGCTATTGCACCTAAGG
CAGAGTATCAAATAAAAAACACAGGAGAATTAGAATCTCAGTTAGAAATGACAAAAAAGCTTGAAATGAAGTATGACGAT
TATCTCCATCTAATGGAATATGCAGTCAGTTTAAATTTAGATGTTTTTTCTACCCCTTTTGACGAAGACTCTATTGATTT
TTTAGCATCTTTGAAACAAAAAATATGGAAAATCCCTTCAGGTGAGTTATTGAATTTACCGTATCTTGAAAAAATAGCCA
AGCTTCCGATCCCTGATAAGAAAATAATCATATCAACAGGAATGGCTACTATTGATGAGATAAAACAGTCTGTTTCTATT
TTTATAAATAATAAAGTTCCGGTTGATAATATTACAATATTACATTGCAATACTGAATATCCAACGCCCTTTGAGGATGT
AAACCTTAATGCTATTAATGATTTGAAAAAACACTTCCCTAAGAATAACATAGGCTTCTCTGATCATTCTAGCGGGTTTT
ATGCAGCTATTGCGGCGGTGCCTTATGGAATAACTTTTATTGAAAAACATTTCACTTTAGATAAATCTATGTCTGGCCCA
GATCATTTGGCCTCAATAGAACCTGATGAACTGAAACATCTATGTATTGGGGTCAGGTGTGTTGAAAAATCTTTAGGTTC
AAATAGTAAAGTGGTTACAGCTTCAGAAAGGAAGAATAAAATCGTAGCAAGAAAGTCTATTATAGCTAAAACAGAGATAA
AAAAAGGTGAGGTTTTTTCAGAAAAAAATATAACAACAAAAAGACCTGGTAATGGTATCAGTCCGATGGAGTGGTATAAT
TTATTGGGTAAAATTGCAGAGCAAGACTTTATTCCAGATGAATTAATAATTCATAGCGAATTCAAAAATCAGGGGGAATA
A

Upstream 100 bases:

>100_bases
GCTCAAAGAGTATTATTGGTTCTGGGTCGGTTGTAATTAGAAATATACCAAGTAATGTTGTAGTTGCTGGGACTCCAACA
AGATTAATTAGGGGGAATGA

Downstream 100 bases:

>100_bases
TGAGAACAAAAATTATTGCGATAATTCCAGCCCGTAGTGGATCTAAAGGGTTGAGAAATAAAAATGCTTTGATGCTGATA
GATAAACCTCTTCTTGCTTA

Product: polysialic acid capsule biosynthesis sialic acid synthase NeuB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKADKLISAIAPKAEYQIKNTGELESQLEMTKKLEMKYDD
YLHLMEYAVSLNLDVFSTPFDEDSIDFLASLKQKIWKIPSGELLNLPYLEKIAKLPIPDKKIIISTGMATIDEIKQSVSI
FINNKVPVDNITILHCNTEYPTPFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYGITFIEKHFTLDKSMSGP
DHLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKIVARKSIIAKTEIKKGEVFSEKNITTKRPGNGISPMEWYN
LLGKIAEQDFIPDELIIHSEFKNQGE

Sequences:

>Translated_346_residues
MSNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKADKLISAIAPKAEYQIKNTGELESQLEMTKKLEMKYDD
YLHLMEYAVSLNLDVFSTPFDEDSIDFLASLKQKIWKIPSGELLNLPYLEKIAKLPIPDKKIIISTGMATIDEIKQSVSI
FINNKVPVDNITILHCNTEYPTPFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYGITFIEKHFTLDKSMSGP
DHLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKIVARKSIIAKTEIKKGEVFSEKNITTKRPGNGISPMEWYN
LLGKIAEQDFIPDELIIHSEFKNQGE
>Mature_345_residues
SNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKADKLISAIAPKAEYQIKNTGELESQLEMTKKLEMKYDDY
LHLMEYAVSLNLDVFSTPFDEDSIDFLASLKQKIWKIPSGELLNLPYLEKIAKLPIPDKKIIISTGMATIDEIKQSVSIF
INNKVPVDNITILHCNTEYPTPFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYGITFIEKHFTLDKSMSGPD
HLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKIVARKSIIAKTEIKKGEVFSEKNITTKRPGNGISPMEWYNL
LGKIAEQDFIPDELIIHSEFKNQGE

Specific function: Unknown

COG id: COG2089

COG function: function code M; Sialic acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis spsE [H]

Homologues:

Organism=Homo sapiens, GI12056473, Length=340, Percent_Identity=36.4705882352941, Blast_Score=198, Evalue=8e-51,
Organism=Drosophila melanogaster, GI21357081, Length=367, Percent_Identity=32.1525885558583, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006190
- InterPro:   IPR013785
- InterPro:   IPR013132
- InterPro:   IPR013974 [H]

Pfam domain/function: PF03102 NeuB; PF08666 SAF [H]

EC number: NA

Molecular weight: Translated: 38752; Mature: 38621

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50844 AFP_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKADKLISAIAPKAEYQIKN
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCEEECC
TGELESQLEMTKKLEMKYDDYLHLMEYAVSLNLDVFSTPFDEDSIDFLASLKQKIWKIPS
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
GELLNLPYLEKIAKLPIPDKKIIISTGMATIDEIKQSVSIFINNKVPVDNITILHCNTEY
CCEECCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCCCEEEEEECCCC
PTPFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYGITFIEKHFTLDKSMSGP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCHHHHHHHHCCCCCCCCC
DHLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKIVARKSIIAKTEIKKGEVFS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHCHHHHHHHHHHHHCCCCCCEEC
EKNITTKRPGNGISPMEWYNLLGKIAEQDFIPDELIIHSEFKNQGE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
SNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKADKLISAIAPKAEYQIKN
CCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCEEECC
TGELESQLEMTKKLEMKYDDYLHLMEYAVSLNLDVFSTPFDEDSIDFLASLKQKIWKIPS
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
GELLNLPYLEKIAKLPIPDKKIIISTGMATIDEIKQSVSIFINNKVPVDNITILHCNTEY
CCEECCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCCCEEEEEECCCC
PTPFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYGITFIEKHFTLDKSMSGP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCHHHHHHHHCCCCCCCCC
DHLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKIVARKSIIAKTEIKKGEVFS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHCHHHHHHHHHHHHCCCCCCEEC
EKNITTKRPGNGISPMEWYNLLGKIAEQDFIPDELIIHSEFKNQGE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]