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The map label for this gene is alsE [H]

Identifier: 170679730

GI number: 170679730

Start: 4653391

End: 4654086

Strand: Reverse

Name: alsE [H]

Synonym: EcSMS35_4551

Alternate gene names: 170679730

Gene position: 4654086-4653391 (Counterclockwise)

Preceding gene: 170684183

Following gene: 170680209

Centisome position: 91.83

GC content: 48.13

Gene sequence:

>696_bases
ATGAAAATCTCCCCCTCGTTAATGTGTATGGATTTGCTGAAATTTAAAGAACAGATCGAATTTATCGACAGCCATGCCGA
TTACTTTCACATCGATATCATGGACGGTCACTTTGTGCCGAATCTGACGCTCTCACCGTTCTTCGTAAGCCAGGTAAAAA
AACTGGCGAGTAAACCGCTCGACTGCCATCTGATGGTAACGCGTCCGCAGGATTACATTGCGCAACTGGCGCGTGCGGGA
GCTGATTTCATCACTTTGCATCCGGAAACTATCAACGGTCAGGCATTCCGCCTGATTGATGAAATCCGCCGTCATGACAT
GAAAGTGGGGCTAATCCTTAACCCGGAGACGCCAGTTGAGGCCATGAAATACTATATCCATAAGGCCGATAAAATTACGG
TCATGACTGTCGATCCCGGTTTTGCCGGACAACCGTTCATTCCTGAAATGCTGGATAAACTTGCCGAACTGAAGGCATGG
CGTGAACGAGAAGGTCTGGAGTACGAAATTGAGGTGGACGGTTCCTGCAACCAGGCAACTTACGAAAAACTAATGGCGGC
AGGGGCGGATGTCTTTATAGTCGGCACTTCCGGCCTGTTTAATCATGCGGAAAATATCGACGAAGCATGGAGAATTATGA
CCGCGCAGATTCTGGCTGCAAAAAGCGAGGTACAGCCTCATGCAAAAACAGCATAA

Upstream 100 bases:

>100_bases
ATTTTGCAGGTACAAACCTATTACCAACTGGTGGTGATGGGCGGATTAATTATCGCGGCTGTCGCCCTTGACCGTCTTAT
CAGTAAGTAAGGAATTGAAC

Downstream 100 bases:

>100_bases
CGTCGTAGCGGGCGTGGATATGGGGGCAACGCATATCCGCTTTTGTCTGCGGACAGCAGAAGGTGAAACGCTACACTGCG
AAAAAAAGCGGACCGCAGAA

Product: allulose-6-phosphate 3-epimerase

Products: fructose-6-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTLSPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAG
ADFITLHPETINGQAFRLIDEIRRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQILAAKSEVQPHAKTA

Sequences:

>Translated_231_residues
MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTLSPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAG
ADFITLHPETINGQAFRLIDEIRRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQILAAKSEVQPHAKTA
>Mature_231_residues
MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTLSPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAG
ADFITLHPETINGQAFRLIDEIRRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQILAAKSEVQPHAKTA

Specific function: D-ALLOSE METABOLISM. ESSENTIAL FOR THIS PATHWAY. [C]

COG id: COG0036

COG function: function code G; Pentose-5-phosphate-3-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribulose-phosphate 3-epimerase family [H]

Homologues:

Organism=Homo sapiens, GI40385883, Length=199, Percent_Identity=39.1959798994975, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI219879828, Length=199, Percent_Identity=37.1859296482412, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI24307923, Length=153, Percent_Identity=32.0261437908497, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1790523, Length=231, Percent_Identity=99.5670995670996, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789788, Length=197, Percent_Identity=37.0558375634518, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790754, Length=160, Percent_Identity=32.5, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17552948, Length=200, Percent_Identity=36, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6322341, Length=209, Percent_Identity=33.4928229665072, Blast_Score=119, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24586301, Length=198, Percent_Identity=36.3636363636364, Blast_Score=130, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000056
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00834 Ribul_P_3_epim [H]

EC number: 5.1.3.- [C]

Molecular weight: Translated: 26095; Mature: 26095

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS01085 RIBUL_P_3_EPIMER_1 ; PS01086 RIBUL_P_3_EPIMER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTLSPFFVSQVKKLASKPL
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCC
DCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEIRRHDMKVGLILNPETPVE
CEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHH
AMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAWREREGLEYEIEVDGSCNQAT
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
YEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQILAAKSEVQPHAKTA
HHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTLSPFFVSQVKKLASKPL
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCC
DCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEIRRHDMKVGLILNPETPVE
CEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHH
AMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAWREREGLEYEIEVDGSCNQAT
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
YEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQILAAKSEVQPHAKTA
HHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-allulose-6-phosphate [C]

Specific reaction: D-allulose-6-phosphate = fructose-6-phosphate [C]

General reaction: Isomerases; Racemases and Epimerases; Acting on Carbohydrates and Derivatives [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 9401019 [H]