Click here to switch to the map view.

The map label for this gene is alsC [H]

Identifier: 170684183

GI number: 170684183

Start: 4654097

End: 4655077

Strand: Reverse

Name: alsC [H]

Synonym: EcSMS35_4552

Alternate gene names: 170684183

Gene position: 4655077-4654097 (Counterclockwise)

Preceding gene: 170683675

Following gene: 170679730

Centisome position: 91.85

GC content: 52.4

Gene sequence:

>981_bases
ATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACCGTTCAACTTTGCGCTGTTCTGGGATAAATACGG
CACCTTTTTTATCCTGGCGATCATCGTCGCCATCTTTGGTTCGCTGTCACCAGAATATTTTCTGACCACCAATAATATTA
CCCAGATTTTTGTGCAAAGCTCCGTGACGGTATTGATCGGCATGGGCGAGTTTTTCGCTATCCTGGTCGCTGGTATCGAC
CTCTCGGTTGGCGCGATTCTGGCGCTTTCCGGTATGGTGACCGCCAAACTGATGTTGGCAGGTGTTGACCCGTTTCTCGC
GGCGCTGATTGGCGGTGTACTGGTTGGCGGCGCACTGGGGGCGATCAACGGCTGCCTGGTCAACTGGACGGGGCTACACC
CATTTATTATCACCCTTGGTACCAACGCCATTTTCCGTGGGATCACGCTGGTGATCTCCGATGCCAACTCGGTATACGGC
TTCTCATTTGACTTCGTGAACTTCTTTGCCGCCAGCGTAATTGGGATACCTGTTCCCGTTATCTTCTCGCTAATTGTCGC
GCTCATCCTTTGGTTTCTGACAACGCGTATGCGGCTCGGACGCAACATCTACGCACTGGGCGGCAACAAAAACTCGGCGT
TCTATTCCGGGATTGACGTGAAATTCCACATCCTGGTGGTGTTTATCATCTCCGGTGTTTGTGCAGGTCTGGCAGGCGTC
GTCTCAACTGCACGACTCGGTGCCGCAGAACCGCTTGCCGGTATGGGTTTTGAAACCTATGCCATTGCCAGCGCCATCAT
TGGCGGCACCAGTTTCTTCGGCGGCAAGGGGCGCATTTTCTCTGTGGTGATTGGCGGGTTGATCATCGGCACCATCAACA
ACGGTCTGAATATTTTGCAGGTACAAACCTATTACCAACTGGTGGTGATGGGCGGATTAATTATCGCGGCTGTCGCCCTT
GACCGTCTTATCAGTAAGTAA

Upstream 100 bases:

>100_bases
AAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATG
AGCGAAGAGGAGATTATGGC

Downstream 100 bases:

>100_bases
GGAATTGAACATGAAAATCTCCCCCTCGTTAATGTGTATGGATTTGCTGAAATTTAAAGAACAGATCGAATTTATCGACA
GCCATGCCGATTACTTTCAC

Product: D-allose transporter subunit

Products: ADP; phosphate; ribose [Cytoplasm]; D-allose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSPEYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGID
LSVGAILALSGMVTAKLMLAGVDPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGIDVKFHILVVFIISGVCAGLAGV
VSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGKGRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVAL
DRLISK

Sequences:

>Translated_326_residues
MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSPEYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGID
LSVGAILALSGMVTAKLMLAGVDPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGIDVKFHILVVFIISGVCAGLAGV
VSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGKGRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVAL
DRLISK
>Mature_325_residues
GFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSPEYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDL
SVGAILALSGMVTAKLMLAGVDPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYGF
SFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGIDVKFHILVVFIISGVCAGLAGVV
STARLGAAEPLAGMGFETYAIASAIIGGTSFFGGKGRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALD
RLISK

Specific function: Part of the binding-protein-dependent transport system AlsBAC for D-allose; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1172

COG function: function code G; Ribose/xylose/arabinose/galactoside ABC-type transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. AraH/rbsC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790524, Length=326, Percent_Identity=99.6932515337423, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1790191, Length=315, Percent_Identity=39.0476190476191, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI145693152, Length=318, Percent_Identity=30.188679245283, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1788896, Length=304, Percent_Identity=29.2763157894737, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788471, Length=332, Percent_Identity=33.1325301204819, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789992, Length=135, Percent_Identity=40.7407407407407, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787794, Length=307, Percent_Identity=28.6644951140065, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082395, Length=283, Percent_Identity=32.5088339222615, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI145693214, Length=246, Percent_Identity=33.739837398374, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787793, Length=277, Percent_Identity=27.0758122743682, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 34298; Mature: 34167

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSPEYFLTTNNITQIFVQS
CCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEC
SVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGVDPFLAALIGGVLVGGALG
CEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYGFSFDFVNFFAASVIGIPVPV
HCCCEEEEECCCCEEEEEECCHHHHHCEEEEEECCCCEEEEEHHHHHHHHHHHHCCCHHH
IFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGIDVKFHILVVFIISGVCAGLAGV
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
VSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGKGRIFSVVIGGLIIGTINNGLNILQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHEEEECCCCEEEE
VQTYYQLVVMGGLIIAAVALDRLISK
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSPEYFLTTNNITQIFVQS
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEC
SVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGVDPFLAALIGGVLVGGALG
CEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYGFSFDFVNFFAASVIGIPVPV
HCCCEEEEECCCCEEEEEECCHHHHHCEEEEEECCCCEEEEEHHHHHHHHHHHHCCCHHH
IFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGIDVKFHILVVFIISGVCAGLAGV
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
VSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGKGRIFSVVIGGLIIGTINNGLNILQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHEEEECCCCEEEE
VQTYYQLVVMGGLIIAAVALDRLISK
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; ribose [Periplasm]; D-allose [Periplasm]; ATP [C]

Specific reaction: ATP + H2O + ribose [Periplasm] = ADP + phosphate + ribose [Cytoplasm] D-allose [Periplasm] + ATP + H2O = D-allose [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7610040; 9401019 [H]