Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 170079040

GI number: 170079040

Start: 2538694

End: 2539794

Strand: Reverse

Name: nemA [H]

Synonym: SYNPCC7002_A2445

Alternate gene names: 170079040

Gene position: 2539794-2538694 (Counterclockwise)

Preceding gene: 170079041

Following gene: 170079037

Centisome position: 84.43

GC content: 51.5

Gene sequence:

>1101_bases
ATGACAACGACTTTTGCAACGCTTTTAGAGCCTTTTCACCTCTGGGATCTCCCTTTGACAAACCGTGTAGTAATGGCTCC
CATGACCAGGGCACGGGCTGGCAAAGATCGCATTCCCAACGCGCTCATGGCGGAATACTATGGCCAACGGGCTTCGGCGG
GTCTAATTATTGCAGAGGCCACAACCATTTCAGAACAGGCCAATGGTTGGAATGAATCGCCAGGTGTTTACACGGACGCC
ATGGTTGAAGGATGGCAACTCACCACCCAAGCCGTCCACGCCAAAGGCGGCAAGATTTTTTTGCAATTGTGGCACATGGG
CCGCGCTTCCCATAGCAGTTTCCATGGGGGTCAATTGCCGATCGCCCCTTCCGCAATCAAAATTAATAACGGCGAAGAAA
TTCATACGCCCCTTGGCAAACAGCCCTATGAAGTCCCCAGGGCCATTGAAACAGAAGAAATCCCTGGGATTATTGAGGAG
TATCGCCAGGCGGCAATCAGGGCAAAAGCAGCGGGCTTTGATGGTATTGAGATTCACTCCGCCAACGGCTATTTACTCGA
TACATTCCTCCAATCGAAAACGAACCATCGTACGGATGCCTATGGGGGCAGCGCCACGAATCGTTGTCGTTTACTCCTCG
AGATTTTAGATGCGGTAACAACGGTCTGGGATTCCCGTCGTGTGGGTGTTCGTTTTTCCCCCAATGGTTCCTTTAATGAT
ATGGGTTCGCCCGATTTCCGGGAGCAGTTTACCTATGCTGCCAGTCAACTCAATCGGTACAATTTGGCCTATTTCCATGT
GATGGATGGTTTGGCCTTTGGTTTCCATGAACTGGGAGAGCCGCTGACCTTGGCTGATTTCCGGGCCGTTTATGATGGTG
TTCTCATGGGTAATTGTGGCTATGATCGAAGCAGTGCAGAAACGGCGATCGCCACTGGGCAAGCTGACCTAATTGCCATT
GGCCGTCCTTTTATTAGTAACCCTGACCTCGTTGAACGTTACACCAATAACTGGGCCTTAAACCCCGATGCAGATATAGC
CACTTGGTATGCCCCCACAGGCGCGGAAGGCTATACCGATTTCCCGACCTATGCCGCCTAG

Upstream 100 bases:

>100_bases
TTTGGCTAAGACAGATCTCGGTTTTTAGGAAAATTGTTAAAAAAGTTTACAATGAACAAATATTTTATTGACCAAGTAGT
CAAAAAGAACAATTAAATCT

Downstream 100 bases:

>100_bases
GATGCTGGTCGATTTTCATTGCTGATAGGCAATTTAGCAAAAAGGGCAGCCCTAGGGCTGCCCTTCTCTTATTTATGTTT
TTTATGTTTTAGATTGCGAC

Product: oxidoreductase, FAD/FMN-binding protein

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTTTFATLLEPFHLWDLPLTNRVVMAPMTRARAGKDRIPNALMAEYYGQRASAGLIIAEATTISEQANGWNESPGVYTDA
MVEGWQLTTQAVHAKGGKIFLQLWHMGRASHSSFHGGQLPIAPSAIKINNGEEIHTPLGKQPYEVPRAIETEEIPGIIEE
YRQAAIRAKAAGFDGIEIHSANGYLLDTFLQSKTNHRTDAYGGSATNRCRLLLEILDAVTTVWDSRRVGVRFSPNGSFND
MGSPDFREQFTYAASQLNRYNLAYFHVMDGLAFGFHELGEPLTLADFRAVYDGVLMGNCGYDRSSAETAIATGQADLIAI
GRPFISNPDLVERYTNNWALNPDADIATWYAPTGAEGYTDFPTYAA

Sequences:

>Translated_366_residues
MTTTFATLLEPFHLWDLPLTNRVVMAPMTRARAGKDRIPNALMAEYYGQRASAGLIIAEATTISEQANGWNESPGVYTDA
MVEGWQLTTQAVHAKGGKIFLQLWHMGRASHSSFHGGQLPIAPSAIKINNGEEIHTPLGKQPYEVPRAIETEEIPGIIEE
YRQAAIRAKAAGFDGIEIHSANGYLLDTFLQSKTNHRTDAYGGSATNRCRLLLEILDAVTTVWDSRRVGVRFSPNGSFND
MGSPDFREQFTYAASQLNRYNLAYFHVMDGLAFGFHELGEPLTLADFRAVYDGVLMGNCGYDRSSAETAIATGQADLIAI
GRPFISNPDLVERYTNNWALNPDADIATWYAPTGAEGYTDFPTYAA
>Mature_365_residues
TTTFATLLEPFHLWDLPLTNRVVMAPMTRARAGKDRIPNALMAEYYGQRASAGLIIAEATTISEQANGWNESPGVYTDAM
VEGWQLTTQAVHAKGGKIFLQLWHMGRASHSSFHGGQLPIAPSAIKINNGEEIHTPLGKQPYEVPRAIETEEIPGIIEEY
RQAAIRAKAAGFDGIEIHSANGYLLDTFLQSKTNHRTDAYGGSATNRCRLLLEILDAVTTVWDSRRVGVRFSPNGSFNDM
GSPDFREQFTYAASQLNRYNLAYFHVMDGLAFGFHELGEPLTLADFRAVYDGVLMGNCGYDRSSAETAIATGQADLIAIG
RPFISNPDLVERYTNNWALNPDADIATWYAPTGAEGYTDFPTYAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=44.3243243243243, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1789463, Length=354, Percent_Identity=26.5536723163842, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=281, Percent_Identity=28.8256227758007, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=302, Percent_Identity=29.1390728476821, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17559804, Length=290, Percent_Identity=28.6206896551724, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=276, Percent_Identity=26.4492753623188, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=135, Percent_Identity=37.037037037037, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17564188, Length=78, Percent_Identity=43.5897435897436, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=35.8638743455497, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321973, Length=386, Percent_Identity=34.7150259067358, Blast_Score=180, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40269; Mature: 40137

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTFATLLEPFHLWDLPLTNRVVMAPMTRARAGKDRIPNALMAEYYGQRASAGLIIAEA
CCCCHHHHHCHHHEECCCCCCCEEECCHHHHHCCHHHCCHHHHHHHHCCCCCCCEEEEEE
TTISEQANGWNESPGVYTDAMVEGWQLTTQAVHAKGGKIFLQLWHMGRASHSSFHGGQLP
HHHHHHCCCCCCCCCEEHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCC
IAPSAIKINNGEEIHTPLGKQPYEVPRAIETEEIPGIIEEYRQAAIRAKAAGFDGIEIHS
CCCCEEEECCCCEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLLDTFLQSKTNHRTDAYGGSATNRCRLLLEILDAVTTVWDSRRVGVRFSPNGSFND
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
MGSPDFREQFTYAASQLNRYNLAYFHVMDGLAFGFHELGEPLTLADFRAVYDGVLMGNCG
CCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEECCCC
YDRSSAETAIATGQADLIAIGRPFISNPDLVERYTNNWALNPDADIATWYAPTGAEGYTD
CCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCC
FPTYAA
CCCCCC
>Mature Secondary Structure 
TTTFATLLEPFHLWDLPLTNRVVMAPMTRARAGKDRIPNALMAEYYGQRASAGLIIAEA
CCCHHHHHCHHHEECCCCCCCEEECCHHHHHCCHHHCCHHHHHHHHCCCCCCCEEEEEE
TTISEQANGWNESPGVYTDAMVEGWQLTTQAVHAKGGKIFLQLWHMGRASHSSFHGGQLP
HHHHHHCCCCCCCCCEEHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCC
IAPSAIKINNGEEIHTPLGKQPYEVPRAIETEEIPGIIEEYRQAAIRAKAAGFDGIEIHS
CCCCEEEECCCCEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLLDTFLQSKTNHRTDAYGGSATNRCRLLLEILDAVTTVWDSRRVGVRFSPNGSFND
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
MGSPDFREQFTYAASQLNRYNLAYFHVMDGLAFGFHELGEPLTLADFRAVYDGVLMGNCG
CCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEECCCC
YDRSSAETAIATGQADLIAIGRPFISNPDLVERYTNNWALNPDADIATWYAPTGAEGYTD
CCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCC
FPTYAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]