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The map label for this gene is mnhD1 [H]

Identifier: 170078969

GI number: 170078969

Start: 2456540

End: 2457976

Strand: Direct

Name: mnhD1 [H]

Synonym: SYNPCC7002_A2374

Alternate gene names: 170078969

Gene position: 2456540-2457976 (Clockwise)

Preceding gene: 170078968

Following gene: 170078970

Centisome position: 81.67

GC content: 49.76

Gene sequence:

>1437_bases
ATGATCGACGACATCACAATTATTTGGATTTTGTTGCCGTTTGTTGTGGGGTTCAGTATTTATCTCTTGCCCCGCTGGAA
CCGTTACTTTGCTTTGGCGATCGCCGCTCTATCGGTGGTCTATAGTATCGGTCTGCTTTGGAGTCTAGAGCCTTTTACCC
TAGAACTCTTGGATAGCTTCGGCGTCACCCTGATGTTCGATGAACTGAGCGGCTATTTCATTTTGATGAATGGGCTGGTG
ACGGGGGCTGTACTCCTTTATTGTTTTGACAAACAGAAATCACCCTTTTTCTATACTCAACTGGTGATCCTCCATGGGGC
GGTAAACGCGACGTTTTGCTGTGCAGATTTGATCAGTCTGTATGTCGCCCTAGAGTGCATTGGGATTGCTGCATTTTTGT
TAATCACCTATTCCCGGAGCGATCGCTCCCTCTGGGTCGGCCTGCGCTATCTCTTCATTAGCAATACCGCGATGTTGTTT
TATCTCATTGGTGCGGTATTGGTCTACCAAGCGAGTAATTCCTTTGCTTTTTCGGGTTTGGCGGTGGCCCCCAAGGAGGC
GATCGCCTTAATCTTCTTAGGCTTACTGACCAAAGGGGGCATTTTCGTCTCTGGTCTGTGGCTCCCCCTCACCCATGGGG
AATCAGAAACTCCCGTATCCGCGCTTTTATCAGGGGTTGTGGTTAAAGCTGGCGTTTTTCCCTTGGCCCGCTGCGCTCTA
TTAGTGCCAGAGCTTGATCCTGTGGTGCGATTATTCGGTGTGGGTACGGCTCTGTTGGGGGTTGGCTATGCGGTTTTTGA
GAAAGATACAAAACGAATGTTGGCTTTTCACACCGTTTCCCAATTGGGTTTTGTATTGGCCGCGCCAGCAGTGGGTGGGT
TTTATGCCCTCACCCATGGTCTCGTTAAAGGCGCCCTCTTTCTGACGGCGGGACAGTTATCCAGCCGCAATTTTAAAGTT
TTACGCGAACAGTCGATTCCCCGGGCTTACTGGTGGGTCTTGGTGTTAGCCTGTGCTTCGATCTCTGGTCTTCCTTTTCT
GGCGGGCTACAGTTCTAAAATTCTGACGATGAAAAATATTCTGCCTTGGCAAAGCTTCGCCCTCAATGTGGCGGCAGTGG
GGACAGCAATTTCCTTTGCGAAATTCATTTTCCTCCCGAAAAAAACAGACCCCAGCCTCAAAATTTTGCCGAATTTTTAT
GGCGCGATTGTACTGCTCCTGGGTGGGTTGTTTGTGACCAACAGCTTTTACCTGGAAGCTTATCAACCCAGTAATATCCT
CAAGGCGTTAGTCACCATCGGCATCGGTTGGCTGGCCTATGGGCTGATTTTCCAGCGGATTACGGTCAAGCTGCCCCGGG
TCGTGGAGCAATTTGAACATCTGGTGGGGGTGATGAGTCTTGTGTTGACGGGTTTGTTTTGGCTGGTGTTGGCTTAG

Upstream 100 bases:

>100_bases
TTGGCTTTTCGATTCAGGCCTTGATGCTCGTGGGCGTGATGAAGTTGTCACGGGATAATCCGACCTTAGAAACCAGCGAA
ATCGAGAAAAGCCACACGCC

Downstream 100 bases:

>100_bases
GACAACTCTCAGTGGTCGTTAGTTTAGGGAGGAGATAACCATGAATGAATTGACCATTGGCTGGGTAATTTTTCCCTTTG
TGGTGGGCTTTAGTATTTAT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MIDDITIIWILLPFVVGFSIYLLPRWNRYFALAIAALSVVYSIGLLWSLEPFTLELLDSFGVTLMFDELSGYFILMNGLV
TGAVLLYCFDKQKSPFFYTQLVILHGAVNATFCCADLISLYVALECIGIAAFLLITYSRSDRSLWVGLRYLFISNTAMLF
YLIGAVLVYQASNSFAFSGLAVAPKEAIALIFLGLLTKGGIFVSGLWLPLTHGESETPVSALLSGVVVKAGVFPLARCAL
LVPELDPVVRLFGVGTALLGVGYAVFEKDTKRMLAFHTVSQLGFVLAAPAVGGFYALTHGLVKGALFLTAGQLSSRNFKV
LREQSIPRAYWWVLVLACASISGLPFLAGYSSKILTMKNILPWQSFALNVAAVGTAISFAKFIFLPKKTDPSLKILPNFY
GAIVLLLGGLFVTNSFYLEAYQPSNILKALVTIGIGWLAYGLIFQRITVKLPRVVEQFEHLVGVMSLVLTGLFWLVLA

Sequences:

>Translated_478_residues
MIDDITIIWILLPFVVGFSIYLLPRWNRYFALAIAALSVVYSIGLLWSLEPFTLELLDSFGVTLMFDELSGYFILMNGLV
TGAVLLYCFDKQKSPFFYTQLVILHGAVNATFCCADLISLYVALECIGIAAFLLITYSRSDRSLWVGLRYLFISNTAMLF
YLIGAVLVYQASNSFAFSGLAVAPKEAIALIFLGLLTKGGIFVSGLWLPLTHGESETPVSALLSGVVVKAGVFPLARCAL
LVPELDPVVRLFGVGTALLGVGYAVFEKDTKRMLAFHTVSQLGFVLAAPAVGGFYALTHGLVKGALFLTAGQLSSRNFKV
LREQSIPRAYWWVLVLACASISGLPFLAGYSSKILTMKNILPWQSFALNVAAVGTAISFAKFIFLPKKTDPSLKILPNFY
GAIVLLLGGLFVTNSFYLEAYQPSNILKALVTIGIGWLAYGLIFQRITVKLPRVVEQFEHLVGVMSLVLTGLFWLVLA
>Mature_478_residues
MIDDITIIWILLPFVVGFSIYLLPRWNRYFALAIAALSVVYSIGLLWSLEPFTLELLDSFGVTLMFDELSGYFILMNGLV
TGAVLLYCFDKQKSPFFYTQLVILHGAVNATFCCADLISLYVALECIGIAAFLLITYSRSDRSLWVGLRYLFISNTAMLF
YLIGAVLVYQASNSFAFSGLAVAPKEAIALIFLGLLTKGGIFVSGLWLPLTHGESETPVSALLSGVVVKAGVFPLARCAL
LVPELDPVVRLFGVGTALLGVGYAVFEKDTKRMLAFHTVSQLGFVLAAPAVGGFYALTHGLVKGALFLTAGQLSSRNFKV
LREQSIPRAYWWVLVLACASISGLPFLAGYSSKILTMKNILPWQSFALNVAAVGTAISFAKFIFLPKKTDPSLKILPNFY
GAIVLLLGGLFVTNSFYLEAYQPSNILKALVTIGIGWLAYGLIFQRITVKLPRVVEQFEHLVGVMSLVLTGLFWLVLA

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=311, Percent_Identity=26.0450160771704, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788827, Length=281, Percent_Identity=30.6049822064057, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788831, Length=240, Percent_Identity=26.25, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788829, Length=301, Percent_Identity=26.578073089701, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788614, Length=334, Percent_Identity=29.6407185628743, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788613, Length=361, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 52238; Mature: 52238

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDDITIIWILLPFVVGFSIYLLPRWNRYFALAIAALSVVYSIGLLWSLEPFTLELLDSF
CCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GVTLMFDELSGYFILMNGLVTGAVLLYCFDKQKSPFFYTQLVILHGAVNATFCCADLISL
CCEEEEECCCCEEEEHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
YVALECIGIAAFLLITYSRSDRSLWVGLRYLFISNTAMLFYLIGAVLVYQASNSFAFSGL
HHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCE
AVAPKEAIALIFLGLLTKGGIFVSGLWLPLTHGESETPVSALLSGVVVKAGVFPLARCAL
EECHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHH
LVPELDPVVRLFGVGTALLGVGYAVFEKDTKRMLAFHTVSQLGFVLAAPAVGGFYALTHG
HCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LVKGALFLTAGQLSSRNFKVLREQSIPRAYWWVLVLACASISGLPFLAGYSSKILTMKNI
HHHHHHHEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHC
LPWQSFALNVAAVGTAISFAKFIFLPKKTDPSLKILPNFYGAIVLLLGGLFVTNSFYLEA
CCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCEEEEE
YQPSNILKALVTIGIGWLAYGLIFQRITVKLPRVVEQFEHLVGVMSLVLTGLFWLVLA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDDITIIWILLPFVVGFSIYLLPRWNRYFALAIAALSVVYSIGLLWSLEPFTLELLDSF
CCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GVTLMFDELSGYFILMNGLVTGAVLLYCFDKQKSPFFYTQLVILHGAVNATFCCADLISL
CCEEEEECCCCEEEEHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
YVALECIGIAAFLLITYSRSDRSLWVGLRYLFISNTAMLFYLIGAVLVYQASNSFAFSGL
HHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCE
AVAPKEAIALIFLGLLTKGGIFVSGLWLPLTHGESETPVSALLSGVVVKAGVFPLARCAL
EECHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHH
LVPELDPVVRLFGVGTALLGVGYAVFEKDTKRMLAFHTVSQLGFVLAAPAVGGFYALTHG
HCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LVKGALFLTAGQLSSRNFKVLREQSIPRAYWWVLVLACASISGLPFLAGYSSKILTMKNI
HHHHHHHEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHC
LPWQSFALNVAAVGTAISFAKFIFLPKKTDPSLKILPNFYGAIVLLLGGLFVTNSFYLEA
CCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCEEEEE
YQPSNILKALVTIGIGWLAYGLIFQRITVKLPRVVEQFEHLVGVMSLVLTGLFWLVLA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]