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The map label for this gene is braE [H]

Identifier: 170078699

GI number: 170078699

Start: 2173851

End: 2174747

Strand: Reverse

Name: braE [H]

Synonym: SYNPCC7002_A2098

Alternate gene names: 170078699

Gene position: 2174747-2173851 (Counterclockwise)

Preceding gene: 170078700

Following gene: 170078698

Centisome position: 72.3

GC content: 55.07

Gene sequence:

>897_bases
ATGGCGGCATTTTTGAGTACCTACGGCTATTTGATCGTTTCTACGCTTCTGGGGGCACTGTTGGGGATCTCGGTTTACCT
ACCACTGATGGCGGGTCAGCTTTCTTTGGCGACACCGGGTTTCTATGCCCTGGGGGGTTACATTGCGGCGATCCTCTCGA
CCCAGGCGATCGCCTTTACGGGGACGACCTTTCCACTGCCTTGGCTCCTGGGGGAAATGGCCCTGGCGGGGTTGGCCTCT
GGAATGGTTGCGCTGATCATTGGGGTGCCCGTGCTGCGGTTGCGGGGGATTTATTTGGCGATCGCCACCATCGCCTTAGT
AGAAATTGTCCGGGTATTGTCGCTAAATTTGCCCATCACCGGGGGCGCTGTGGGGATTTTTGGCATTCCCCAACCCTTTC
GCAGTCCCCTGGGTTATTTGTGGTTGGTGGGGCCATTGTTAATTATCGTGGGCGTTCTAGTGTGGCGTCTAGAAAATACG
AAAGTGGGCCGCGCCTTTAACGCCATCCGTGAAGACGAACTCGCCGCTAGTGCCATGGGCATTAACCCGACGCAATATAA
AGTTTTGGCCTTTGTGTTGGGGGCCATTTTAGCGGGTATTGTCGGGGCGATCGCCGCTCATTTTCTCAACACCTGGAATG
CCCGCCAGGGCACCTTTGACGCCAGCATTACCTACCTTGCCTTTGTGTTGGTCGGGGGGAGTCGTACCTTTTTGGGGCCG
ATCCTGGGGGGCATTGTCCTTACGGCGCTCCCGGAAATATTGCGCGGTTTAGGGGATGCCCGGTTAATTTTCTTCGGTTT
GCTGTTGCTGCTGGGGACGATTTTTTATCCCCAGGGTTTGATTACCCCCGAACTGCTACGTCGTTGTTTACCCACTGCCT
TGAAGCCTAAAATCTAA

Upstream 100 bases:

>100_bases
TCAGGGTATCGGGAGGCGATCGCCTTTGCGTTACTGTTTGGGATGTTGCTGCTCCGGCCCCAGGGGTTATTGGGCCAAAA
AACGATTCAAAAGGTGTGAG

Downstream 100 bases:

>100_bases
TTTTTAGCGTTAAATCGCCGCTTTTTCCCCTCCTTTGACCAATTTATCTTGAAATAATGGAAAAAATCACCCTCACCCGC
CCCGATGACTGGCATTTGCA

Product: permease protein of branched-chain amino acid ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAAFLSTYGYLIVSTLLGALLGISVYLPLMAGQLSLATPGFYALGGYIAAILSTQAIAFTGTTFPLPWLLGEMALAGLAS
GMVALIIGVPVLRLRGIYLAIATIALVEIVRVLSLNLPITGGAVGIFGIPQPFRSPLGYLWLVGPLLIIVGVLVWRLENT
KVGRAFNAIREDELAASAMGINPTQYKVLAFVLGAILAGIVGAIAAHFLNTWNARQGTFDASITYLAFVLVGGSRTFLGP
ILGGIVLTALPEILRGLGDARLIFFGLLLLLGTIFYPQGLITPELLRRCLPTALKPKI

Sequences:

>Translated_298_residues
MAAFLSTYGYLIVSTLLGALLGISVYLPLMAGQLSLATPGFYALGGYIAAILSTQAIAFTGTTFPLPWLLGEMALAGLAS
GMVALIIGVPVLRLRGIYLAIATIALVEIVRVLSLNLPITGGAVGIFGIPQPFRSPLGYLWLVGPLLIIVGVLVWRLENT
KVGRAFNAIREDELAASAMGINPTQYKVLAFVLGAILAGIVGAIAAHFLNTWNARQGTFDASITYLAFVLVGGSRTFLGP
ILGGIVLTALPEILRGLGDARLIFFGLLLLLGTIFYPQGLITPELLRRCLPTALKPKI
>Mature_297_residues
AAFLSTYGYLIVSTLLGALLGISVYLPLMAGQLSLATPGFYALGGYIAAILSTQAIAFTGTTFPLPWLLGEMALAGLASG
MVALIIGVPVLRLRGIYLAIATIALVEIVRVLSLNLPITGGAVGIFGIPQPFRSPLGYLWLVGPLLIIVGVLVWRLENTK
VGRAFNAIREDELAASAMGINPTQYKVLAFVLGAILAGIVGAIAAHFLNTWNARQGTFDASITYLAFVLVGGSRTFLGPI
LGGIVLTALPEILRGLGDARLIFFGLLLLLGTIFYPQGLITPELLRRCLPTALKPKI

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=298, Percent_Identity=33.5570469798658, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 31417; Mature: 31286

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFLSTYGYLIVSTLLGALLGISVYLPLMAGQLSLATPGFYALGGYIAAILSTQAIAFT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHEEE
GTTFPLPWLLGEMALAGLASGMVALIIGVPVLRLRGIYLAIATIALVEIVRVLSLNLPIT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGAVGIFGIPQPFRSPLGYLWLVGPLLIIVGVLVWRLENTKVGRAFNAIREDELAASAMG
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
INPTQYKVLAFVLGAILAGIVGAIAAHFLNTWNARQGTFDASITYLAFVLVGGSRTFLGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
ILGGIVLTALPEILRGLGDARLIFFGLLLLLGTIFYPQGLITPELLRRCLPTALKPKI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHCCCCC
>Mature Secondary Structure 
AAFLSTYGYLIVSTLLGALLGISVYLPLMAGQLSLATPGFYALGGYIAAILSTQAIAFT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHEEE
GTTFPLPWLLGEMALAGLASGMVALIIGVPVLRLRGIYLAIATIALVEIVRVLSLNLPIT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGAVGIFGIPQPFRSPLGYLWLVGPLLIIVGVLVWRLENTKVGRAFNAIREDELAASAMG
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
INPTQYKVLAFVLGAILAGIVGAIAAHFLNTWNARQGTFDASITYLAFVLVGGSRTFLGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
ILGGIVLTALPEILRGLGDARLIFFGLLLLLGTIFYPQGLITPELLRRCLPTALKPKI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]