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The map label for this gene is sigF [H]

Identifier: 170078529

GI number: 170078529

Start: 2008610

End: 2009389

Strand: Direct

Name: sigF [H]

Synonym: SYNPCC7002_A1924

Alternate gene names: 170078529

Gene position: 2008610-2009389 (Clockwise)

Preceding gene: 170078528

Following gene: 170078538

Centisome position: 66.77

GC content: 43.97

Gene sequence:

>780_bases
ATGGAACCTACGACGACAAAAGTTAGTGACAATATTAAGTCAAAAACCCTGGAACTGTTACAGGAATATCGGCAGTCCCA
AGATGTCAAAATTCGTAATCAGATTGTAAGTTTAAATATTGGCTTGGTTCGCAAAGAAGCCCACCATTGGTCGAGACAAT
GTAGCGAAAATTTTGATGATCTCATGCAGGTGGGGAGCTTGGGTTTACTCCGGGCCATTGAGCGATTTACCCTAGAGCGG
GGCAACGCTTTCAGTTCCTTTGCGAATCCCTATATCCGCGGTGAAATTCAACATTACCTGCGAGATAAAAGCTCTACTGT
CCGTATTCCCCGCCGAATGTTGCAATTGCGGCAACAGTCTAATCGCTTTATCCACACTTTTCGCCAAGAACATAATCGAC
AACCCTCTGCCATCGAAATCGCTGAACACCTCAACATTTCTGTAACTGAATGGCAATCCATTAATCTGGCTTATCAAAAC
CGTGATCCTGTCAGTCTTGATGTTTCTGTAAAAGCTGACCAAGGGGAAAATACTTCTTTAGGAGATCTGGTTCCGGATCC
GGGTTACAAAAGCTTTCAACTGGCCATTGAAGATCAAATTCGCATTCAACAGGGCCTTGCTCAACTCGAAGAACATACCC
GCAAGGTATTGGAATTTGTTTTTCTCCATGACCTTACCCAAAAAGAGGCCGCTGACCTACTAGGAGTGAGTGTTATTACC
GTGTCACGGCGGATTAAAAAAGGGATTAATTCCCTTAAGAAAATCCTCGGTGGTGATTAG

Upstream 100 bases:

>100_bases
TTAATTTTGAAGTTTTTCATCAGAAAAATAGCGTATCTTTTTTAGTTTGCTCCAATCCATTTCTCAGTAATCGCTTGACC
ATTAATCTGGCCGAAAAATA

Downstream 100 bases:

>100_bases
TTAGAGGCTTACATTTTGGAGGCGATCGCGGGGTTTAAAAGAATTAAGCGCCCGGAGCTTCTCATAACACTCTTTTTCCG
CTTCGGTGGGTGCAGCAGGG

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MEPTTTKVSDNIKSKTLELLQEYRQSQDVKIRNQIVSLNIGLVRKEAHHWSRQCSENFDDLMQVGSLGLLRAIERFTLER
GNAFSSFANPYIRGEIQHYLRDKSSTVRIPRRMLQLRQQSNRFIHTFRQEHNRQPSAIEIAEHLNISVTEWQSINLAYQN
RDPVSLDVSVKADQGENTSLGDLVPDPGYKSFQLAIEDQIRIQQGLAQLEEHTRKVLEFVFLHDLTQKEAADLLGVSVIT
VSRRIKKGINSLKKILGGD

Sequences:

>Translated_259_residues
MEPTTTKVSDNIKSKTLELLQEYRQSQDVKIRNQIVSLNIGLVRKEAHHWSRQCSENFDDLMQVGSLGLLRAIERFTLER
GNAFSSFANPYIRGEIQHYLRDKSSTVRIPRRMLQLRQQSNRFIHTFRQEHNRQPSAIEIAEHLNISVTEWQSINLAYQN
RDPVSLDVSVKADQGENTSLGDLVPDPGYKSFQLAIEDQIRIQQGLAQLEEHTRKVLEFVFLHDLTQKEAADLLGVSVIT
VSRRIKKGINSLKKILGGD
>Mature_259_residues
MEPTTTKVSDNIKSKTLELLQEYRQSQDVKIRNQIVSLNIGLVRKEAHHWSRQCSENFDDLMQVGSLGLLRAIERFTLER
GNAFSSFANPYIRGEIQHYLRDKSSTVRIPRRMLQLRQQSNRFIHTFRQEHNRQPSAIEIAEHLNISVTEWQSINLAYQN
RDPVSLDVSVKADQGENTSLGDLVPDPGYKSFQLAIEDQIRIQQGLAQLEEHTRKVLEFVFLHDLTQKEAADLLGVSVIT
VSRRIKKGINSLKKILGGD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=158, Percent_Identity=29.1139240506329, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788231, Length=224, Percent_Identity=27.6785714285714, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789098, Length=124, Percent_Identity=33.8709677419355, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29730; Mature: 29730

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPTTTKVSDNIKSKTLELLQEYRQSQDVKIRNQIVSLNIGLVRKEAHHWSRQCSENFDD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LMQVGSLGLLRAIERFTLERGNAFSSFANPYIRGEIQHYLRDKSSTVRIPRRMLQLRQQS
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NRFIHTFRQEHNRQPSAIEIAEHLNISVTEWQSINLAYQNRDPVSLDVSVKADQGENTSL
HHHHHHHHHHHCCCCCHHHHHHHCCCEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCC
GDLVPDPGYKSFQLAIEDQIRIQQGLAQLEEHTRKVLEFVFLHDLTQKEAADLLGVSVIT
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSRRIKKGINSLKKILGGD
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPTTTKVSDNIKSKTLELLQEYRQSQDVKIRNQIVSLNIGLVRKEAHHWSRQCSENFDD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LMQVGSLGLLRAIERFTLERGNAFSSFANPYIRGEIQHYLRDKSSTVRIPRRMLQLRQQS
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NRFIHTFRQEHNRQPSAIEIAEHLNISVTEWQSINLAYQNRDPVSLDVSVKADQGENTSL
HHHHHHHHHHHCCCCCHHHHHHHCCCEEECCEEEEEEECCCCCEEEEEEEECCCCCCCCC
GDLVPDPGYKSFQLAIEDQIRIQQGLAQLEEHTRKVLEFVFLHDLTQKEAADLLGVSVIT
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSRRIKKGINSLKKILGGD
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]