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The map label for this gene is thiO/thiG [H]

Identifier: 170078432

GI number: 170078432

Start: 1911285

End: 1913267

Strand: Direct

Name: thiO/thiG [H]

Synonym: SYNPCC7002_A1826

Alternate gene names: 170078432

Gene position: 1911285-1913267 (Clockwise)

Preceding gene: 170078431

Following gene: 170078433

Centisome position: 63.54

GC content: 54.01

Gene sequence:

>1983_bases
ATGGAAAAAATGGCACAGCAAGCGGACGTTTTAATTTTAGGAGGCGGGATCATTGGTTTGGCGATCGCCGTCGAATTGCA
ACAACAGGGTTGTGCCGTGACAATCCTGAATCGAAACTTTACCGAGGCTGCCTGCCATGCCGCTGCCGGAATGCTCGCCC
CGGAGGCCGAAGGGTTAACAGGAACGATGCTCGAATTGGGGCGGCGATCGCGCAATATGTACGCCGACTGGTGCCGCAAA
ATTGAACAACTCAGCGGCGTCGATGTGGGTTATTTGCCCTGTGGTATTTTTGCTCCCAAATCCGTTGCACCGAGCCAAAC
CGTGACTGATAGCGCTTCTGTTTGGCTAGACCGCGCCCAACTGACCTACCATGAACCGGGTTTTGGTGATGGGGTGGTGG
GAGCCTGGTGGCACCCCGAAGATGGCCAAGTAGACAACCGCCGTCTGAGCCAAGCCTTACTCCAAGCGGCACGGGGTCTG
GGAGTGGTAATTAAAGAAGGCGTTACTGTTTTGGGTCTCCAACAAATCCAAGGACAGGTGCAAGCGGTGCAGACCGATCA
AGGGCTATTCCAAGCGGGCCATTATGTTTTGGCCGCAGGTTCCTGGTCGAGTCAGATCACGCCCCTGTCCGTTTATCCGA
TTAAAGGGGAAATGATGTCCCTGAGGATGCCGAATCCTGAGGCCCTCCGGCGGGTGATTTTTGGTGATAATCTGTACCTC
GTGCCGCGCACCAATGGGACCCTAATCATTGGGGCTACCGCAGAAACTGTGAAGTGGCAACCCAACAATACCCCCCAAGG
GATCCAAACCCTGCTGAATAAAGCGATTCAACTGTTGCCGAAACTCAAAGATTGGGAAATCACGGAACTCTGGTGGGGTT
TTCGGCCCGCAACCCCCGATGAAAAGCCGATTCTGGGGGCATCCCCTTGTGAAAATCTCACCTACGCCACGGGCCATTAT
CGCAATGGCATTTTATTGGCTCCCATCACGGCGAAACTCATTGGCGATCGCCTAATCCGCAACCAAGCAGATCCCCTCCT
CCAACATTTTTCTTACCAACGCTTTTTAAATAACCCAACCCATCAACCCATGCAAAACGGTCACAACAGTCAGAATGCTC
AAAACGGTTCAATGCCGAGCTTTTTTATCAGCCCCAAAACCACAACTGCAACGCCAAACCCCACGGAAACCCCTGATCAA
CTGGTCATTGCCGGTCGTCGCTTTACTTCCCGGTTAATGACAGGAACGGGGAAATATCCCTCAATTGCCGCAATGCAAGC
AAGCGTTGAAGCCAGCGGTTGCGAAATTGTCACGGTGGCAGTGCGTCGCGTCCAAACCAAAGCCCCAGGCCACGAAGGAC
TTGCCGAAGCCCTCGACTGGCAAAAAATTTGGATGTTGCCTAATACGGCGGGCTGTAAAACTGCCGAGGAAGCGATCCGG
GTAGCTCGTTTAGGTCGGGAAATGGCCCGTCTGTTAGGCCAAGAAGATAACAACTTTGTCAAATTAGAAGTGATCCCCGA
TGCCCGCTACCTACTGCCGGATCCCATCGGTACCCTCGACGCCGCAGAACAATTGGTCAAAGAAGGGTTTGCGGTGCTGC
CCTATATTAATGCCGATCCTCTGTTGGCGAAACGCTTAGAGGAAGTGGGCTGTGCCACGGTGATGCCCCTCGGTTCTCCC
ATTGGGTCGGGCCAGGGGATTCAAAATGAAGCCAACATCAAAATTATTATTGAACAGAGCAAGGTTCCCGTGGTGATTGA
TGCGGGGATTGGCTCCCCTAGTGAAGCGGCCTACGGGATGGAATTGGGAGCCGATGCGCTACTGGTCAATTCGGCGATCG
CCCTCGCTAAAGATCCCGTACAAATGGGTCGAGCCATGGGGTTAGCCACCACTGCCGGCCGCCTCGCTCACCTAGCCGGG
CGCATTCCGGTTCAACATATTGCCAGTGCCAGTTCTCCCCAAACTGGACTGGTCAGCACCTAA

Upstream 100 bases:

>100_bases
CTGCTACTTGGAGTGGGGATTTTTTATTTACAAGTCAAGGTTGCCGCAAAAATCTTGGCCGTTGTAGCTTAAAGGAAGGC
ACCGGAAGGAATTTAAGGTG

Downstream 100 bases:

>100_bases
GTTCCTCCTGTCGATTGAATGCGGCGATCGCATCATCAATATTCTGATTTCGCAAAAGCAAGCCAGGCCCGTAGTTAACA
CTACAAAATCTCCCTGACTG

Product: bifunctional glycine oxidase (ThiO) and thiazole biosynthesis protein ThiG

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MEKMAQQADVLILGGGIIGLAIAVELQQQGCAVTILNRNFTEAACHAAAGMLAPEAEGLTGTMLELGRRSRNMYADWCRK
IEQLSGVDVGYLPCGIFAPKSVAPSQTVTDSASVWLDRAQLTYHEPGFGDGVVGAWWHPEDGQVDNRRLSQALLQAARGL
GVVIKEGVTVLGLQQIQGQVQAVQTDQGLFQAGHYVLAAGSWSSQITPLSVYPIKGEMMSLRMPNPEALRRVIFGDNLYL
VPRTNGTLIIGATAETVKWQPNNTPQGIQTLLNKAIQLLPKLKDWEITELWWGFRPATPDEKPILGASPCENLTYATGHY
RNGILLAPITAKLIGDRLIRNQADPLLQHFSYQRFLNNPTHQPMQNGHNSQNAQNGSMPSFFISPKTTTATPNPTETPDQ
LVIAGRRFTSRLMTGTGKYPSIAAMQASVEASGCEIVTVAVRRVQTKAPGHEGLAEALDWQKIWMLPNTAGCKTAEEAIR
VARLGREMARLLGQEDNNFVKLEVIPDARYLLPDPIGTLDAAEQLVKEGFAVLPYINADPLLAKRLEEVGCATVMPLGSP
IGSGQGIQNEANIKIIIEQSKVPVVIDAGIGSPSEAAYGMELGADALLVNSAIALAKDPVQMGRAMGLATTAGRLAHLAG
RIPVQHIASASSPQTGLVST

Sequences:

>Translated_660_residues
MEKMAQQADVLILGGGIIGLAIAVELQQQGCAVTILNRNFTEAACHAAAGMLAPEAEGLTGTMLELGRRSRNMYADWCRK
IEQLSGVDVGYLPCGIFAPKSVAPSQTVTDSASVWLDRAQLTYHEPGFGDGVVGAWWHPEDGQVDNRRLSQALLQAARGL
GVVIKEGVTVLGLQQIQGQVQAVQTDQGLFQAGHYVLAAGSWSSQITPLSVYPIKGEMMSLRMPNPEALRRVIFGDNLYL
VPRTNGTLIIGATAETVKWQPNNTPQGIQTLLNKAIQLLPKLKDWEITELWWGFRPATPDEKPILGASPCENLTYATGHY
RNGILLAPITAKLIGDRLIRNQADPLLQHFSYQRFLNNPTHQPMQNGHNSQNAQNGSMPSFFISPKTTTATPNPTETPDQ
LVIAGRRFTSRLMTGTGKYPSIAAMQASVEASGCEIVTVAVRRVQTKAPGHEGLAEALDWQKIWMLPNTAGCKTAEEAIR
VARLGREMARLLGQEDNNFVKLEVIPDARYLLPDPIGTLDAAEQLVKEGFAVLPYINADPLLAKRLEEVGCATVMPLGSP
IGSGQGIQNEANIKIIIEQSKVPVVIDAGIGSPSEAAYGMELGADALLVNSAIALAKDPVQMGRAMGLATTAGRLAHLAG
RIPVQHIASASSPQTGLVST
>Mature_660_residues
MEKMAQQADVLILGGGIIGLAIAVELQQQGCAVTILNRNFTEAACHAAAGMLAPEAEGLTGTMLELGRRSRNMYADWCRK
IEQLSGVDVGYLPCGIFAPKSVAPSQTVTDSASVWLDRAQLTYHEPGFGDGVVGAWWHPEDGQVDNRRLSQALLQAARGL
GVVIKEGVTVLGLQQIQGQVQAVQTDQGLFQAGHYVLAAGSWSSQITPLSVYPIKGEMMSLRMPNPEALRRVIFGDNLYL
VPRTNGTLIIGATAETVKWQPNNTPQGIQTLLNKAIQLLPKLKDWEITELWWGFRPATPDEKPILGASPCENLTYATGHY
RNGILLAPITAKLIGDRLIRNQADPLLQHFSYQRFLNNPTHQPMQNGHNSQNAQNGSMPSFFISPKTTTATPNPTETPDQ
LVIAGRRFTSRLMTGTGKYPSIAAMQASVEASGCEIVTVAVRRVQTKAPGHEGLAEALDWQKIWMLPNTAGCKTAEEAIR
VARLGREMARLLGQEDNNFVKLEVIPDARYLLPDPIGTLDAAEQLVKEGFAVLPYINADPLLAKRLEEVGCATVMPLGSP
IGSGQGIQNEANIKIIIEQSKVPVVIDAGIGSPSEAAYGMELGADALLVNSAIALAKDPVQMGRAMGLATTAGRLAHLAG
RIPVQHIASASSPQTGLVST

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=258, Percent_Identity=54.2635658914729, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1787438, Length=263, Percent_Identity=26.615969581749, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 70905; Mature: 70905

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMAQQADVLILGGGIIGLAIAVELQQQGCAVTILNRNFTEAACHAAAGMLAPEAEGLT
CCCHHHHCCEEEECCCCEEEHEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC
GTMLELGRRSRNMYADWCRKIEQLSGVDVGYLPCGIFAPKSVAPSQTVTDSASVWLDRAQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCE
LTYHEPGFGDGVVGAWWHPEDGQVDNRRLSQALLQAARGLGVVIKEGVTVLGLQQIQGQV
EEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHCHH
QAVQTDQGLFQAGHYVLAAGSWSSQITPLSVYPIKGEMMSLRMPNPEALRRVIFGDNLYL
EEEECCCHHHHCCCEEEEECCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHCCCCEEE
VPRTNGTLIIGATAETVKWQPNNTPQGIQTLLNKAIQLLPKLKDWEITELWWGFRPATPD
EECCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCC
EKPILGASPCENLTYATGHYRNGILLAPITAKLIGDRLIRNQADPLLQHFSYQRFLNNPT
CCCCCCCCCCCCCCEECCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
HQPMQNGHNSQNAQNGSMPSFFISPKTTTATPNPTETPDQLVIAGRRFTSRLMTGTGKYP
CCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCC
SIAAMQASVEASGCEIVTVAVRRVQTKAPGHEGLAEALDWQKIWMLPNTAGCKTAEEAIR
CHHHHEEECCCCCCEEEEHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHH
VARLGREMARLLGQEDNNFVKLEVIPDARYLLPDPIGTLDAAEQLVKEGFAVLPYINADP
HHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCEEEEECCCCH
LLAKRLEEVGCATVMPLGSPIGSGQGIQNEANIKIIIEQSKVPVVIDAGIGSPSEAAYGM
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCH
ELGADALLVNSAIALAKDPVQMGRAMGLATTAGRLAHLAGRIPVQHIASASSPQTGLVST
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEKMAQQADVLILGGGIIGLAIAVELQQQGCAVTILNRNFTEAACHAAAGMLAPEAEGLT
CCCHHHHCCEEEECCCCEEEHEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC
GTMLELGRRSRNMYADWCRKIEQLSGVDVGYLPCGIFAPKSVAPSQTVTDSASVWLDRAQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCE
LTYHEPGFGDGVVGAWWHPEDGQVDNRRLSQALLQAARGLGVVIKEGVTVLGLQQIQGQV
EEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEHHHHHCHH
QAVQTDQGLFQAGHYVLAAGSWSSQITPLSVYPIKGEMMSLRMPNPEALRRVIFGDNLYL
EEEECCCHHHHCCCEEEEECCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHCCCCEEE
VPRTNGTLIIGATAETVKWQPNNTPQGIQTLLNKAIQLLPKLKDWEITELWWGFRPATPD
EECCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCC
EKPILGASPCENLTYATGHYRNGILLAPITAKLIGDRLIRNQADPLLQHFSYQRFLNNPT
CCCCCCCCCCCCCCEECCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
HQPMQNGHNSQNAQNGSMPSFFISPKTTTATPNPTETPDQLVIAGRRFTSRLMTGTGKYP
CCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCC
SIAAMQASVEASGCEIVTVAVRRVQTKAPGHEGLAEALDWQKIWMLPNTAGCKTAEEAIR
CHHHHEEECCCCCCEEEEHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHH
VARLGREMARLLGQEDNNFVKLEVIPDARYLLPDPIGTLDAAEQLVKEGFAVLPYINADP
HHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCEEEEECCCCH
LLAKRLEEVGCATVMPLGSPIGSGQGIQNEANIKIIIEQSKVPVVIDAGIGSPSEAAYGM
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCH
ELGADALLVNSAIALAKDPVQMGRAMGLATTAGRLAHLAGRIPVQHIASASSPQTGLVST
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]