Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 170078390

GI number: 170078390

Start: 1869583

End: 1871289

Strand: Direct

Name: nadE [H]

Synonym: SYNPCC7002_A1784

Alternate gene names: 170078390

Gene position: 1869583-1871289 (Clockwise)

Preceding gene: 170078389

Following gene: 170078394

Centisome position: 62.15

GC content: 55.13

Gene sequence:

>1707_bases
ATGAAAATTGCGATCGCCCAACTGAACCCCACCATCGGGGACTTGACTGGTAATGCCGAGCGGATCCTGGTCGCAGCAGA
AGAAGCGGCGATCGCCAATGTCCGCTTATTGCTCACGCCGGAACTGTCCCCTCTGTGGGTATCCCGCCCCCGGGATCTCT
TGCTCCATGCGGATTTTATTGACCGGATGCAGGCCCAGCTCACGACCGTGGCGGCAAAAATTCCGCCGACCCTCGCCGTT
TTAGTCGGTTTGGCGACTCCCAACCCAGAGGCGATCGCCAAGGGAGAAAAAGCCCTCCACAACAGCATCGCCCTCATTGA
CCAGGGGAAAGTCCAGCACATTTTCCATAAGCAACTGTTGCCCACCTACGATGTGTTCGACGAAGACCGCTATTTTGAAC
CCGGCAAGCAGTCCAACTGGTTTTTCCTCCATCCTAGCCCGACGGTCGCCCCTTTAAAAATTGGCGTTACCATCTGCGAA
GATCTCTGGAATGACCAAGCCTTTTGGGGCAAGCGCCAGTACGAAATTAATCCCCTCGAACAATTGGCCGAGGCCGGGGT
CGATTTAATCGTGAACTTGTCGGCTTCTCCCTACACCGTCGGAAAACAGCGCCTGCGGGAAGGGATGCTCCACCATGGCG
CTCAACGTTACCGGACGCCCATTCTCTACGCGAACCAAGTGGGGGGGAATGACGATTTGATTTTTGACGGCGGGAGTGTG
GCCTTTGACCGAGAGGGACAACTGGTTGGGCGATCGCCAAGTTTTCAAGAGTCCCTGTTGGCGGTGGAATTTAGCCCCGA
CCAACAAACCCTATCACTGCCCCAGGGTTTACCTGCTGCTTTTCAGCAAACCATTAGTCCCGAATATGATGCCGATGAGG
CGGAAATTTTTGCGGCCCTGGTGTTGGGTCTCCGGGACTATGCCCGTAAATGCGGCTTTTCGAAAATTGTCCTGGGTTTG
AGTGGTGGCATCGACTCGGCCTTGGTGGCGGCGATCGCCGTTGCAGCGGTGGGGGCTGAAAATGTGTTGGGGGTGCTCAT
GCCTTCCCCCTACAGTTCCGAACATTCCCTCAGCGATGCGATCGCCCTGGTGGAAAATCTCCAGATTCGCCACGAAATTA
TTCCCATTGAGCCAGCGATGCAAGCCTTTGAGACGATGCTGGGTGATTTATTTGCGGGCACAGAATTTGGAGTAGCCGAA
GAAAATCTCCAGTCCCGCATCCGGGGAAATCTGCTGATGGCGATCGCCAATAAATTTGGTTATCTGCTGGTGTCAACGGG
CAACAAGTCCGAAATGGCGGTGGGTTATTGCACCCTCTATGGCGATATGAATGGCGGCCTGGCGGTGATTGCTGATGTCC
CGAAAATGCGGGTGTTTAGCCTTTGTCAATGGTTAAACCGTGACCGAGAACTCATTCCCCCGGCAATTATTACCAAACCC
CCTAGTGCGGAACTGCGTCCCGACCAATTGGATCAAGACTCCTTGCCCCCCTATCCGGTGTTGGATGAGATCCTCAAACG
GGTCATCCACCGCAATCAATCGGCCCCAGAACTCCACGCCGCTGGCTATGACCACGATACTGTGGCCCAGGTTTTAAAGC
TCCTCCATCGCGCCGAATTTAAGCGCCGTCAAGCTGCCCCTGGTTTAAAAATTACCGATCGAGCCTTTGGTACGGGGTGG
CGGATGCCCATTGCCTGCCGCTGGTGA

Upstream 100 bases:

>100_bases
GAAGTCGGTTTTGATTACCGCACCACCGTCGCCAGTGATCCGCGCCACCGCACGATCATTGCCCACAAGCCCTTTTAATT
GTTTGCCAAATGTTTGCCGA

Downstream 100 bases:

>100_bases
AGCAGATCCCAAACATTAACCAACCCCAGAGATTTCAGTTGATTCCCAGGGAGCCAAACCGAGGTAACAAATGCCCTCTG
GACTAACCGCAAAGCGACAG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKIAIAQLNPTIGDLTGNAERILVAAEEAAIANVRLLLTPELSPLWVSRPRDLLLHADFIDRMQAQLTTVAAKIPPTLAV
LVGLATPNPEAIAKGEKALHNSIALIDQGKVQHIFHKQLLPTYDVFDEDRYFEPGKQSNWFFLHPSPTVAPLKIGVTICE
DLWNDQAFWGKRQYEINPLEQLAEAGVDLIVNLSASPYTVGKQRLREGMLHHGAQRYRTPILYANQVGGNDDLIFDGGSV
AFDREGQLVGRSPSFQESLLAVEFSPDQQTLSLPQGLPAAFQQTISPEYDADEAEIFAALVLGLRDYARKCGFSKIVLGL
SGGIDSALVAAIAVAAVGAENVLGVLMPSPYSSEHSLSDAIALVENLQIRHEIIPIEPAMQAFETMLGDLFAGTEFGVAE
ENLQSRIRGNLLMAIANKFGYLLVSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKMRVFSLCQWLNRDRELIPPAIITKP
PSAELRPDQLDQDSLPPYPVLDEILKRVIHRNQSAPELHAAGYDHDTVAQVLKLLHRAEFKRRQAAPGLKITDRAFGTGW
RMPIACRW

Sequences:

>Translated_568_residues
MKIAIAQLNPTIGDLTGNAERILVAAEEAAIANVRLLLTPELSPLWVSRPRDLLLHADFIDRMQAQLTTVAAKIPPTLAV
LVGLATPNPEAIAKGEKALHNSIALIDQGKVQHIFHKQLLPTYDVFDEDRYFEPGKQSNWFFLHPSPTVAPLKIGVTICE
DLWNDQAFWGKRQYEINPLEQLAEAGVDLIVNLSASPYTVGKQRLREGMLHHGAQRYRTPILYANQVGGNDDLIFDGGSV
AFDREGQLVGRSPSFQESLLAVEFSPDQQTLSLPQGLPAAFQQTISPEYDADEAEIFAALVLGLRDYARKCGFSKIVLGL
SGGIDSALVAAIAVAAVGAENVLGVLMPSPYSSEHSLSDAIALVENLQIRHEIIPIEPAMQAFETMLGDLFAGTEFGVAE
ENLQSRIRGNLLMAIANKFGYLLVSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKMRVFSLCQWLNRDRELIPPAIITKP
PSAELRPDQLDQDSLPPYPVLDEILKRVIHRNQSAPELHAAGYDHDTVAQVLKLLHRAEFKRRQAAPGLKITDRAFGTGW
RMPIACRW
>Mature_568_residues
MKIAIAQLNPTIGDLTGNAERILVAAEEAAIANVRLLLTPELSPLWVSRPRDLLLHADFIDRMQAQLTTVAAKIPPTLAV
LVGLATPNPEAIAKGEKALHNSIALIDQGKVQHIFHKQLLPTYDVFDEDRYFEPGKQSNWFFLHPSPTVAPLKIGVTICE
DLWNDQAFWGKRQYEINPLEQLAEAGVDLIVNLSASPYTVGKQRLREGMLHHGAQRYRTPILYANQVGGNDDLIFDGGSV
AFDREGQLVGRSPSFQESLLAVEFSPDQQTLSLPQGLPAAFQQTISPEYDADEAEIFAALVLGLRDYARKCGFSKIVLGL
SGGIDSALVAAIAVAAVGAENVLGVLMPSPYSSEHSLSDAIALVENLQIRHEIIPIEPAMQAFETMLGDLFAGTEFGVAE
ENLQSRIRGNLLMAIANKFGYLLVSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKMRVFSLCQWLNRDRELIPPAIITKP
PSAELRPDQLDQDSLPPYPVLDEILKRVIHRNQSAPELHAAGYDHDTVAQVLKLLHRAEFKRRQAAPGLKITDRAFGTGW
RMPIACRW

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=598, Percent_Identity=24.4147157190635, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 62326; Mature: 62326

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIAQLNPTIGDLTGNAERILVAAEEAAIANVRLLLTPELSPLWVSRPRDLLLHADFI
CEEEEEECCCCHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHEEEHHHH
DRMQAQLTTVAAKIPPTLAVLVGLATPNPEAIAKGEKALHNSIALIDQGKVQHIFHKQLL
HHHHHHHHHHHHHCCCCEEEHEECCCCCHHHHHHHHHHHHHCEEEEECCHHHHHHHHHCC
PTYDVFDEDRYFEPGKQSNWFFLHPSPTVAPLKIGVTICEDLWNDQAFWGKRQYEINPLE
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCHHH
QLAEAGVDLIVNLSASPYTVGKQRLREGMLHHGAQRYRTPILYANQVGGNDDLIFDGGSV
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCEEECCCCE
AFDREGQLVGRSPSFQESLLAVEFSPDQQTLSLPQGLPAAFQQTISPEYDADEAEIFAAL
EECCCCCEECCCCCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHH
VLGLRDYARKCGFSKIVLGLSGGIDSALVAAIAVAAVGAENVLGVLMPSPYSSEHSLSDA
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCHHHHHH
IALVENLQIRHEIIPIEPAMQAFETMLGDLFAGTEFGVAEENLQSRIRGNLLMAIANKFG
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHEEEHHHCC
YLLVSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKMRVFSLCQWLNRDRELIPPAIITKP
EEEEECCCCCCEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHEECC
PSAELRPDQLDQDSLPPYPVLDEILKRVIHRNQSAPELHAAGYDHDTVAQVLKLLHRAEF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRRQAAPGLKITDRAFGTGWRMPIACRW
HHHHCCCCCEEECCCCCCCCCCCEEECC
>Mature Secondary Structure
MKIAIAQLNPTIGDLTGNAERILVAAEEAAIANVRLLLTPELSPLWVSRPRDLLLHADFI
CEEEEEECCCCHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHEEEHHHH
DRMQAQLTTVAAKIPPTLAVLVGLATPNPEAIAKGEKALHNSIALIDQGKVQHIFHKQLL
HHHHHHHHHHHHHCCCCEEEHEECCCCCHHHHHHHHHHHHHCEEEEECCHHHHHHHHHCC
PTYDVFDEDRYFEPGKQSNWFFLHPSPTVAPLKIGVTICEDLWNDQAFWGKRQYEINPLE
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCHHH
QLAEAGVDLIVNLSASPYTVGKQRLREGMLHHGAQRYRTPILYANQVGGNDDLIFDGGSV
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCEEECCCCE
AFDREGQLVGRSPSFQESLLAVEFSPDQQTLSLPQGLPAAFQQTISPEYDADEAEIFAAL
EECCCCCEECCCCCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHH
VLGLRDYARKCGFSKIVLGLSGGIDSALVAAIAVAAVGAENVLGVLMPSPYSSEHSLSDA
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCHHHHHH
IALVENLQIRHEIIPIEPAMQAFETMLGDLFAGTEFGVAEENLQSRIRGNLLMAIANKFG
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHEEEHHHCC
YLLVSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKMRVFSLCQWLNRDRELIPPAIITKP
EEEEECCCCCCEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHEECC
PSAELRPDQLDQDSLPPYPVLDEILKRVIHRNQSAPELHAAGYDHDTVAQVLKLLHRAEF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRRQAAPGLKITDRAFGTGWRMPIACRW
HHHHCCCCCEEECCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]