Click here to switch to the map view.

The map label for this gene is metK

Identifier: 170078321

GI number: 170078321

Start: 1800572

End: 1801825

Strand: Reverse

Name: metK

Synonym: SYNPCC7002_A1714

Alternate gene names: 170078321

Gene position: 1801825-1800572 (Counterclockwise)

Preceding gene: 170078322

Following gene: 170078319

Centisome position: 59.9

GC content: 50.48

Gene sequence:

>1254_bases
TTGTCTCGTCGTTATCTCTTTACCTCCGAATCCGTCACCGAGGGACATCCCGATAAAATTTGTGACCAAATCTCCGATGC
AATTTTAGATGCCGTACTTGCTGCTGATCCCAGTAGCCGTGTTGCTGCTGAGGTTGTGACCAATACCGGTTTGGTGCTGA
TTACGGGTGAACTAACAACCCAAGCGAATGTGAATTTTATCCAGGTGGCTCGCCAAAAAATCAAAGAAATTGGCTACACC
AATGCCGATAATGGCTTTTCTGCCAATAGCTGCTCTGTGATGGTTGCCCTGGATGAACAATCCCCCGACATCGCCCAGGG
GGTCACCAGCGCCCAGGAGCAACGGGACAAACTCAGTGACGACCAACTCGATGAAATTGGTGCCGGAGACCAGGGGTTAA
TGTTTGGCTATGCCTGCAACGAGACACCCGAATTCATGCCCCTCCCGATTAGTTTGGCCCATCGTTTGTCTCTGAAATTG
AGCCAAGTGCGCAAAAGCGGTGAACTGCCTTACCTCGGCCCCGACGGTAAAACCCAAGTAACGGTGATCTACGAGGACGG
CAAACCCGTGGGGATCGATACGATTTTGATTTCGACCCAACACACAGAGACCATTGGGGCACTCACAGACAATACGGAGA
TTCAGGCCAAGATCAAAGCGGATCTGTTGGAAAAAGTAATTAAACCTGTTTTTGTCGGGGCAACGGTGGGTTTAAGTGAC
AGTACGAAGTTCCTGGTAAATCCCACTGGCAAATTTGTCATTGGTGGCCCCCAGGGAGATTCTGGACTTACGGGCCGCAA
GATTATTATCGATACCTACGGTGGCTATTCACGTCACGGGGGCGGTGCTTTCTCCGGCAAAGATCCCACTAAGGTAGATC
GTAGTGCTTCCTATGCTTGCCGTTATGTGGCGAAAAATATTGTCGCAGCGGGCCTTGCGGAAAAATGTGAGGTGCAGGTG
AGCTACGCGATTGGGGTGGCTCGTCCAGTGAGTGTGATGATCGAGACCTTTGGTACTTGCACTGTGGATGAGGATCGGTT
GCTTGAAGTCGTGCAGAAACATTTTGAACTGCGTCCGGCGGGGATTATCCAAGCTTTCGATCTGCGCAATTTACCGAGCC
AACGTAATGGGCGTTTTTATCAGGATATGGCCGCCTATGGACATTTTGGTCGGACAGATTTAGATCTTCCCTGGGAACGT
TTGGACAAGGTAGATATTCTCAAACAGGAACTGGCCACGGCAACAGTTGCCTAG

Upstream 100 bases:

>100_bases
AGAAACTCCCGAATTCTGAAAGAGAAAGGAACTCAACCAGATAAAATAAAAAGCCACTACCCCGCTTGTCCACTTTCTAA
ACTCAAGAGGATATACAACA

Downstream 100 bases:

>100_bases
TTTTCTTGCGTACTGACGTCCTAAACCTAAAAAGCTAATTAAATTTCCCCTGGTGCTTCTTCTGGCAGTGGGGGATTTTT
GTTGAGTTGGCGATCGCCGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSRRYLFTSESVTEGHPDKICDQISDAILDAVLAADPSSRVAAEVVTNTGLVLITGELTTQANVNFIQVARQKIKEIGYT
NADNGFSANSCSVMVALDEQSPDIAQGVTSAQEQRDKLSDDQLDEIGAGDQGLMFGYACNETPEFMPLPISLAHRLSLKL
SQVRKSGELPYLGPDGKTQVTVIYEDGKPVGIDTILISTQHTETIGALTDNTEIQAKIKADLLEKVIKPVFVGATVGLSD
STKFLVNPTGKFVIGGPQGDSGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSASYACRYVAKNIVAAGLAEKCEVQV
SYAIGVARPVSVMIETFGTCTVDEDRLLEVVQKHFELRPAGIIQAFDLRNLPSQRNGRFYQDMAAYGHFGRTDLDLPWER
LDKVDILKQELATATVA

Sequences:

>Translated_417_residues
MSRRYLFTSESVTEGHPDKICDQISDAILDAVLAADPSSRVAAEVVTNTGLVLITGELTTQANVNFIQVARQKIKEIGYT
NADNGFSANSCSVMVALDEQSPDIAQGVTSAQEQRDKLSDDQLDEIGAGDQGLMFGYACNETPEFMPLPISLAHRLSLKL
SQVRKSGELPYLGPDGKTQVTVIYEDGKPVGIDTILISTQHTETIGALTDNTEIQAKIKADLLEKVIKPVFVGATVGLSD
STKFLVNPTGKFVIGGPQGDSGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSASYACRYVAKNIVAAGLAEKCEVQV
SYAIGVARPVSVMIETFGTCTVDEDRLLEVVQKHFELRPAGIIQAFDLRNLPSQRNGRFYQDMAAYGHFGRTDLDLPWER
LDKVDILKQELATATVA
>Mature_416_residues
SRRYLFTSESVTEGHPDKICDQISDAILDAVLAADPSSRVAAEVVTNTGLVLITGELTTQANVNFIQVARQKIKEIGYTN
ADNGFSANSCSVMVALDEQSPDIAQGVTSAQEQRDKLSDDQLDEIGAGDQGLMFGYACNETPEFMPLPISLAHRLSLKLS
QVRKSGELPYLGPDGKTQVTVIYEDGKPVGIDTILISTQHTETIGALTDNTEIQAKIKADLLEKVIKPVFVGATVGLSDS
TKFLVNPTGKFVIGGPQGDSGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSASYACRYVAKNIVAAGLAEKCEVQVS
YAIGVARPVSVMIETFGTCTVDEDRLLEVVQKHFELRPAGIIQAFDLRNLPSQRNGRFYQDMAAYGHFGRTDLDLPWERL
DKVDILKQELATATVA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=399, Percent_Identity=52.3809523809524, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI4557737, Length=399, Percent_Identity=52.1303258145363, Blast_Score=378, Evalue=1e-105,
Organism=Escherichia coli, GI1789311, Length=405, Percent_Identity=56.2962962962963, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25145633, Length=413, Percent_Identity=51.3317191283293, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=416, Percent_Identity=51.2019230769231, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=402, Percent_Identity=51.9900497512438, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551082, Length=404, Percent_Identity=47.029702970297, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565905, Length=402, Percent_Identity=47.7611940298507, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI25145635, Length=362, Percent_Identity=48.6187845303867, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI32565911, Length=368, Percent_Identity=48.6413043478261, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI32565903, Length=327, Percent_Identity=50.7645259938838, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=54.7619047619048, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=74.3589743589744, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=409, Percent_Identity=48.6552567237164, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323209, Length=404, Percent_Identity=49.2574257425743, Blast_Score=388, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45552159, Length=406, Percent_Identity=46.7980295566502, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580529, Length=406, Percent_Identity=46.7980295566502, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580537, Length=406, Percent_Identity=46.7980295566502, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580533, Length=406, Percent_Identity=46.7980295566502, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580535, Length=406, Percent_Identity=46.7980295566502, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580531, Length=406, Percent_Identity=46.7980295566502, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24580527, Length=406, Percent_Identity=46.0591133004926, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580525, Length=406, Percent_Identity=46.0591133004926, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580541, Length=393, Percent_Identity=44.7837150127226, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=48.8188976377953, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_SYNP2 (B1XPB0)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734959.1
- ProteinModelPortal:   B1XPB0
- SMR:   B1XPB0
- GeneID:   6057208
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1714
- HOGENOM:   HBG443662
- OMA:   QDEHARV
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 45041; Mature: 44910

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRYLFTSESVTEGHPDKICDQISDAILDAVLAADPSSRVAAEVVTNTGLVLITGELTT
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCC
QANVNFIQVARQKIKEIGYTNADNGFSANSCSVMVALDEQSPDIAQGVTSAQEQRDKLSD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
DQLDEIGAGDQGLMFGYACNETPEFMPLPISLAHRLSLKLSQVRKSGELPYLGPDGKTQV
CHHHHHCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEE
TVIYEDGKPVGIDTILISTQHTETIGALTDNTEIQAKIKADLLEKVIKPVFVGATVGLSD
EEEEECCCCCCEEEEEEECCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHEECEEECCCC
STKFLVNPTGKFVIGGPQGDSGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSASYAC
CCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RYVAKNIVAAGLAEKCEVQVSYAIGVARPVSVMIETFGTCTVDEDRLLEVVQKHFELRPA
HHHHHHHHHHCCCHHCEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GIIQAFDLRNLPSQRNGRFYQDMAAYGHFGRTDLDLPWERLDKVDILKQELATATVA
HHEEEHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRRYLFTSESVTEGHPDKICDQISDAILDAVLAADPSSRVAAEVVTNTGLVLITGELTT
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCC
QANVNFIQVARQKIKEIGYTNADNGFSANSCSVMVALDEQSPDIAQGVTSAQEQRDKLSD
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
DQLDEIGAGDQGLMFGYACNETPEFMPLPISLAHRLSLKLSQVRKSGELPYLGPDGKTQV
CHHHHHCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEE
TVIYEDGKPVGIDTILISTQHTETIGALTDNTEIQAKIKADLLEKVIKPVFVGATVGLSD
EEEEECCCCCCEEEEEEECCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHEECEEECCCC
STKFLVNPTGKFVIGGPQGDSGLTGRKIIIDTYGGYSRHGGGAFSGKDPTKVDRSASYAC
CCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RYVAKNIVAAGLAEKCEVQVSYAIGVARPVSVMIETFGTCTVDEDRLLEVVQKHFELRPA
HHHHHHHHHHCCCHHCEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GIIQAFDLRNLPSQRNGRFYQDMAAYGHFGRTDLDLPWERLDKVDILKQELATATVA
HHEEEHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA