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The map label for this gene is pyk [H]

Identifier: 170078266

GI number: 170078266

Start: 1741287

End: 1743083

Strand: Direct

Name: pyk [H]

Synonym: SYNPCC7002_A1658

Alternate gene names: 170078266

Gene position: 1741287-1743083 (Clockwise)

Preceding gene: 170078265

Following gene: 170078267

Centisome position: 57.89

GC content: 53.48

Gene sequence:

>1797_bases
ATGCCCTCTAGAGAAATGCCCCGCCGCACTAAGATCGTCGCCACCATTGGCCCTGCGACTAGCAAACCAGACGTTCTCCG
GGAAATCATCGAAGCAGGGGCAACCACTCTACGGCTAAATTTCTCCCATGGTACCCACGATGACCACCAACGCAACATCC
GTCTGATTCGACAAACCGCCTTTGAACTGAATCAACCCGTTGGCATTCTCCAGGATCTCCAGGGGCCAAAAATTCGCCTT
GGTCGCTATGAGAGCGACTTTATCACCCTTAAAAACGGCGATCCCTACATCATCACAAGTCGCGAAGTACCCTGTACCCA
AGAAGTGGGCTATGTCAGCTATGACAAGCTAGCGGAAGAAGTCCCCGAAGGCTCCACCATCCTCCTCGACGATGGCAAGG
TAGAAATGAAGGTCGAACGGGTCGATATTGCCGCAAAAAATCTCCACTGCCGCGTGGTCGTCGGTGGCAAACTCTCCAAT
AACAAAGGGGTCAATTTTCCTGGGGTCTATCTCTCCGTCAAAGCCCTCACCGACAAAGACCGCGAAGATCTCATGTTTGG
CCTCGACCAAGGGGTCGATTGGGTGGCCCTCAGTTTTGTGCGCAATCCCCAAGACGTCCTGGAAATCAAAGAGCTCATCG
CCAATGCTGGGAAAAATGTCCCCGTGATCGTCAAAATTGAAAAACACGAGGCGATCGAACAAATGGAGGCTATTTTGTCC
CTTTCCGATGGTGTTATGGTCGCCCGGGGGGATCTTGGGGTCGAGCTGCCCGCCGAAGACGTACCCATTCTCCAGAAAAA
ACTCATTGCCACCGCCAACCGCTTCGGGATTCCTGTGATTACCGCCACCCAGATGCTCGATAGCATGGTGAGTAATCCTC
GCCCCACCCGCGCCGAAGTTTCCGATGTGGCCAATGCGATTTTGGATGGTACCGATGCGGTGATGCTTTCTAATGAAACT
GCTGTGGGTGAATTCCCCGTAGAAGCCGTGGCGACCATGGCAACCATCGCCAAGCGCATCGAAAAGGAACCCGACGGCAT
TCAAAAACAACCTTCTGATAAAAAATCGATTCCCAATGCTATTTCTGCCGCCGTCAGCCACATTGCTGGACAGTTAGATG
CAACGGCGATCATGACCCTCACCAAGTCTGGGGCAACGGCCCGAAACGTCTCCAAATTTCGCCCAGCAACGCCGATCCTT
GCTGTCACCCCCCATGTGGATGTGGCTCGTCGTCTCCAGTTGGTCTGGGGGGCAAAGCCACTCCTCGTTATGGATTCTCC
GTCAACGACCCAAACTTTTAAGGCGGCGATTAATGTGGCCCAGGAGAGCGGCTTTCTCCATGAAGGGGACTTGGTGATCA
TGACAGCGGGGACGCTCCAAGGGGTGTCTGGTTCCACAGATCTCATCAAAGTCGAAATGGTGCAGGCGGTGCTCGGAGAA
GGCATTGGCATTGGTCAAGGGGCGGCCAGCGGTCGTGCCCGGGTCGTGAATCAAAGTGAAGATATTAATGATTTTGGGCC
AGGGGAGATCCTCGTTGCTCGGTCTACGGATGCGGCCTATGTGGAAATGATCCGTAAGGCCAGCGGCATCGTGATCGAAG
AACCAAATCAAAATTGCCATGCAGCAACTTTGGGGATGCGGTTGGGTATTCCGGTGATTGTCGGTTTTAAGGAAGCAACC
CAGTTGATTCGGGATGGCTCGATTATCTCCATTGATGCCCGTCGAGGCACTGTTTATTCTGGGGTCAATGTGAGCCTTGG
GGATGCTGTGGGTGGTACCAAAATGTCCCTAGCTTAA

Upstream 100 bases:

>100_bases
CCCAAAATCTCCCAGAACTCCCCTGCCACGTATAGAAAGAAACGTCAACTTTTGGGATAATATGAGGCTTGTTTTCCCAA
CTGACCCGAGAAAGTTCACT

Downstream 100 bases:

>100_bases
GCCCAGGCGATCGCAATTATCACTTTTATCATCCGGCGGCCCCAACAATGTTTGGGGCAATTTTTTTGGGTTGGAACAAT
TACGATATCCTAGGGGGTGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MPSREMPRRTKIVATIGPATSKPDVLREIIEAGATTLRLNFSHGTHDDHQRNIRLIRQTAFELNQPVGILQDLQGPKIRL
GRYESDFITLKNGDPYIITSREVPCTQEVGYVSYDKLAEEVPEGSTILLDDGKVEMKVERVDIAAKNLHCRVVVGGKLSN
NKGVNFPGVYLSVKALTDKDREDLMFGLDQGVDWVALSFVRNPQDVLEIKELIANAGKNVPVIVKIEKHEAIEQMEAILS
LSDGVMVARGDLGVELPAEDVPILQKKLIATANRFGIPVITATQMLDSMVSNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGEFPVEAVATMATIAKRIEKEPDGIQKQPSDKKSIPNAISAAVSHIAGQLDATAIMTLTKSGATARNVSKFRPATPIL
AVTPHVDVARRLQLVWGAKPLLVMDSPSTTQTFKAAINVAQESGFLHEGDLVIMTAGTLQGVSGSTDLIKVEMVQAVLGE
GIGIGQGAASGRARVVNQSEDINDFGPGEILVARSTDAAYVEMIRKASGIVIEEPNQNCHAATLGMRLGIPVIVGFKEAT
QLIRDGSIISIDARRGTVYSGVNVSLGDAVGGTKMSLA

Sequences:

>Translated_598_residues
MPSREMPRRTKIVATIGPATSKPDVLREIIEAGATTLRLNFSHGTHDDHQRNIRLIRQTAFELNQPVGILQDLQGPKIRL
GRYESDFITLKNGDPYIITSREVPCTQEVGYVSYDKLAEEVPEGSTILLDDGKVEMKVERVDIAAKNLHCRVVVGGKLSN
NKGVNFPGVYLSVKALTDKDREDLMFGLDQGVDWVALSFVRNPQDVLEIKELIANAGKNVPVIVKIEKHEAIEQMEAILS
LSDGVMVARGDLGVELPAEDVPILQKKLIATANRFGIPVITATQMLDSMVSNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGEFPVEAVATMATIAKRIEKEPDGIQKQPSDKKSIPNAISAAVSHIAGQLDATAIMTLTKSGATARNVSKFRPATPIL
AVTPHVDVARRLQLVWGAKPLLVMDSPSTTQTFKAAINVAQESGFLHEGDLVIMTAGTLQGVSGSTDLIKVEMVQAVLGE
GIGIGQGAASGRARVVNQSEDINDFGPGEILVARSTDAAYVEMIRKASGIVIEEPNQNCHAATLGMRLGIPVIVGFKEAT
QLIRDGSIISIDARRGTVYSGVNVSLGDAVGGTKMSLA
>Mature_597_residues
PSREMPRRTKIVATIGPATSKPDVLREIIEAGATTLRLNFSHGTHDDHQRNIRLIRQTAFELNQPVGILQDLQGPKIRLG
RYESDFITLKNGDPYIITSREVPCTQEVGYVSYDKLAEEVPEGSTILLDDGKVEMKVERVDIAAKNLHCRVVVGGKLSNN
KGVNFPGVYLSVKALTDKDREDLMFGLDQGVDWVALSFVRNPQDVLEIKELIANAGKNVPVIVKIEKHEAIEQMEAILSL
SDGVMVARGDLGVELPAEDVPILQKKLIATANRFGIPVITATQMLDSMVSNPRPTRAEVSDVANAILDGTDAVMLSNETA
VGEFPVEAVATMATIAKRIEKEPDGIQKQPSDKKSIPNAISAAVSHIAGQLDATAIMTLTKSGATARNVSKFRPATPILA
VTPHVDVARRLQLVWGAKPLLVMDSPSTTQTFKAAINVAQESGFLHEGDLVIMTAGTLQGVSGSTDLIKVEMVQAVLGEG
IGIGQGAASGRARVVNQSEDINDFGPGEILVARSTDAAYVEMIRKASGIVIEEPNQNCHAATLGMRLGIPVIVGFKEATQ
LIRDGSIISIDARRGTVYSGVNVSLGDAVGGTKMSLA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=40.081799591002, Blast_Score=329, Evalue=5e-90,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=39.6728016359918, Blast_Score=328, Evalue=9e-90,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=39.6728016359918, Blast_Score=328, Evalue=9e-90,
Organism=Homo sapiens, GI32967597, Length=497, Percent_Identity=38.6317907444668, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=38.3064516129032, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=41.0344827586207, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=41.0344827586207, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=37.0833333333333, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=37.0833333333333, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=36.405529953917, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=42.3728813559322, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788160, Length=483, Percent_Identity=40.3726708074534, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI1787994, Length=100, Percent_Identity=33, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17544584, Length=491, Percent_Identity=39.918533604888, Blast_Score=334, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=39.553752535497, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=39.553752535497, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=39.553752535497, Blast_Score=302, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=39.553752535497, Blast_Score=301, Evalue=7e-82,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=40.2061855670103, Blast_Score=315, Evalue=9e-87,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=37.8099173553719, Blast_Score=295, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24648964, Length=495, Percent_Identity=38.5858585858586, Blast_Score=322, Evalue=7e-88,
Organism=Drosophila melanogaster, GI28571814, Length=495, Percent_Identity=38.5858585858586, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=38.8206388206388, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=32.3711340206186, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24646914, Length=332, Percent_Identity=36.144578313253, Blast_Score=189, Evalue=6e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 64063; Mature: 63932

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSREMPRRTKIVATIGPATSKPDVLREIIEAGATTLRLNFSHGTHDDHQRNIRLIRQTA
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FELNQPVGILQDLQGPKIRLGRYESDFITLKNGDPYIITSREVPCTQEVGYVSYDKLAEE
HHHCCCHHHHHHCCCCCEEECEECCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHH
VPEGSTILLDDGKVEMKVERVDIAAKNLHCRVVVGGKLSNNKGVNFPGVYLSVKALTDKD
CCCCCEEEEECCCEEEEEEEEEEEECCEEEEEEECCEECCCCCCCCCCEEEEEEECCCCC
REDLMFGLDQGVDWVALSFVRNPQDVLEIKELIANAGKNVPVIVKIEKHEAIEQMEAILS
HHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LSDGVMVARGDLGVELPAEDVPILQKKLIATANRFGIPVITATQMLDSMVSNPRPTRAEV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGEFPVEAVATMATIAKRIEKEPDGIQKQPSDKKSIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHH
ISAAVSHIAGQLDATAIMTLTKSGATARNVSKFRPATPILAVTPHVDVARRLQLVWGAKP
HHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCC
LLVMDSPSTTQTFKAAINVAQESGFLHEGDLVIMTAGTLQGVSGSTDLIKVEMVQAVLGE
EEEECCCCCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHC
GIGIGQGAASGRARVVNQSEDINDFGPGEILVARSTDAAYVEMIRKASGIVIEEPNQNCH
CCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEECCCCCCE
AATLGMRLGIPVIVGFKEATQLIRDGSIISIDARRGTVYSGVNVSLGDAVGGTKMSLA
EEEEHHCCCCEEEEEHHHHHHHHHCCCEEEEECCCCEEECCCCEEECCCCCCCEEECC
>Mature Secondary Structure 
PSREMPRRTKIVATIGPATSKPDVLREIIEAGATTLRLNFSHGTHDDHQRNIRLIRQTA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FELNQPVGILQDLQGPKIRLGRYESDFITLKNGDPYIITSREVPCTQEVGYVSYDKLAEE
HHHCCCHHHHHHCCCCCEEECEECCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHH
VPEGSTILLDDGKVEMKVERVDIAAKNLHCRVVVGGKLSNNKGVNFPGVYLSVKALTDKD
CCCCCEEEEECCCEEEEEEEEEEEECCEEEEEEECCEECCCCCCCCCCEEEEEEECCCCC
REDLMFGLDQGVDWVALSFVRNPQDVLEIKELIANAGKNVPVIVKIEKHEAIEQMEAILS
HHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
LSDGVMVARGDLGVELPAEDVPILQKKLIATANRFGIPVITATQMLDSMVSNPRPTRAEV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGEFPVEAVATMATIAKRIEKEPDGIQKQPSDKKSIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHH
ISAAVSHIAGQLDATAIMTLTKSGATARNVSKFRPATPILAVTPHVDVARRLQLVWGAKP
HHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCC
LLVMDSPSTTQTFKAAINVAQESGFLHEGDLVIMTAGTLQGVSGSTDLIKVEMVQAVLGE
EEEECCCCCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHC
GIGIGQGAASGRARVVNQSEDINDFGPGEILVARSTDAAYVEMIRKASGIVIEEPNQNCH
CCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEECCCCCCE
AATLGMRLGIPVIVGFKEATQLIRDGSIISIDARRGTVYSGVNVSLGDAVGGTKMSLA
EEEEHHCCCCEEEEEHHHHHHHHHCCCEEEEECCCCEEECCCCEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]