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The map label for this gene is proS

Identifier: 170078264

GI number: 170078264

Start: 1736176

End: 1737981

Strand: Reverse

Name: proS

Synonym: SYNPCC7002_A1656

Alternate gene names: 170078264

Gene position: 1737981-1736176 (Counterclockwise)

Preceding gene: 170078269

Following gene: 170078263

Centisome position: 57.78

GC content: 51.38

Gene sequence:

>1806_bases
ATGCGCCTTTCCCAAAGTCTGTTCGTTACCCTCCGAGAAGATCCCGCCGATGCGGAGATTCCTAGCCATAAGCTCCTGCT
GCGGGCCGGCTATATCCGGCGGATTGGGCGGGGGATTTATGCTTATCTACCGTTAATGTGGCGCGTGCTCCAGAAGGTAT
CCCAGATCGTCCGGGAAGAGATGAATGCCACCGGTGCCCAGGAAACCCTGTTACCCCAGTTGCAACCCGCAGAGATTTGG
CAAGAATCGGGCCGTTGGGATACCTATACCCAGGCAGAAGGGATTATGTTTTCCCTGCGCGATCGCCTGGATGGAGAGTT
GGGTTTAGGGCCGACCCACGAGGAAGTGATTACCACCATTGCCAAGGACATGATCCGCTCCTATCGGCAGTTGCCCCAGC
ATTTGTACCAGATTCAGACGAAATTCCGCGATGAGATTCGACCCCGGTTTGGCCTGATGCGCGGGCGGGAATTTATCATG
AAAGACGGCTACTCGTTCCATGCTTCCGAGGAATGCCTCAAAAAAACCTACGCAGACATGGATCAGGCCTACCGCAATAT
GTTGCGTCGCTGTGGTTTACAATTCCGGGCGGTAGAAGCAGACTCTGGGGCCATTGGTGGTTCTGGTTCCCAGGAATTTA
TGATCCTCGCCGATGCCGGGGAAGACGAAATTCTCTACACCGAAGACGAAAAGTATGCCGCCAATACCGAGAAAGCCGTG
TCTCTACCCGTTGAGGCGATCGCCTCTCCCTTCAACAGTTTTGAAAAGCGTGAAACCCCGAACACAGCCACCATCGAATC
CCTGTGTAAGTTCCTAAATTGTTCCCCCACTTGTGTCGTTAAAAATGTCCTTTATCAGGTGGTTTATAACAATGGCAAAA
CAGTTTTAGCCTTAATTTCAATTCGAGGCGATCAAGATGTCAACGAAGTTAAATTGCAAAATGAATTGACAAAATTAGCG
CCTAATTACGATGCCAAAACTGTCATTTCCTTAACAGTTCCCGATGCCGATGCTCAACAAAAATGGGCCGCAAAATCATT
ACCTTTAGGCTACATTTCCCCAGCTTTAGCAGATGATTGTATTGCTAAAAATAAAGCGGTTTCAGGCGAATTTCTGCGCT
TAGTTGATCCCACTGCGGCGACATTAGAAAACTTTGTCACGGGTGCCGATGAAACGAATTACCATGTCCTCGGTGCAAAC
TGGGGCACAGAGTTTAAGCTACCTCCATTGCAAGTGGATGTGCGGTTAGCAAAGGCAGGCGATCGCGCTGTCCATGATCC
GACCCAAATCCTCCAAACAGCGCGGGGCATCGAAGCGGGTCACATTTTCCAACTGGGGACAAAATATTCCGAGGCTATGG
GGGCCACCTTTACCGACGAAAATGGTAAAGAACACCCCCTGGTGATGGGCTGCTATGGGGTTGGCGTTTCCCGTCTGGCC
CAGGCCGCCGTGGAGCAATCCTACGATGAAAATGGCATTATCTGGCCCGTGGCGATCGCCCCCTACCATGCCGTGGTAGT
CGTGCCCAATGTTAAGAGTGAAGAACAGATGGCAGCCGCAGAAAAACTCTATGCTGATCTCAACGCCGCTGGGGTTGAAA
CCATCCTTGATGACCGCAACGAACGAGCTGGGGTGAAGTTTAAGGATGCAGAACTGATTGGTATTCCCTTCCGGGTTGTC
ACAGGGAAATCCCTCAAGGATGGCAAGGTGGAAGTGGTGCGCCGCAAAGAAGGCGATCGCCAAGATCTTGATCTCGATGC
TGTGGTTGCGACCTTAAAAAATTGGGTCGAAGCCGCAGGCCAGTAA

Upstream 100 bases:

>100_bases
ACGACATTTTGACCAGGCCCCCGATTCTAAAATGGCAGCAAGTAATCCATAATGGGCGATGGTTCATTCCATTAGCTGTC
ACGAAAAACTCAAAATAACC

Downstream 100 bases:

>100_bases
GCCCCTGGGGAATCGTTATAATGGCCCTAGGGTTAAGCGATTTCCCCTTTTTTAGCCATGGTTCAAACGCCTAGGATTCC
CGACATCACCTACGACCAAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MRLSQSLFVTLREDPADAEIPSHKLLLRAGYIRRIGRGIYAYLPLMWRVLQKVSQIVREEMNATGAQETLLPQLQPAEIW
QESGRWDTYTQAEGIMFSLRDRLDGELGLGPTHEEVITTIAKDMIRSYRQLPQHLYQIQTKFRDEIRPRFGLMRGREFIM
KDGYSFHASEECLKKTYADMDQAYRNMLRRCGLQFRAVEADSGAIGGSGSQEFMILADAGEDEILYTEDEKYAANTEKAV
SLPVEAIASPFNSFEKRETPNTATIESLCKFLNCSPTCVVKNVLYQVVYNNGKTVLALISIRGDQDVNEVKLQNELTKLA
PNYDAKTVISLTVPDADAQQKWAAKSLPLGYISPALADDCIAKNKAVSGEFLRLVDPTAATLENFVTGADETNYHVLGAN
WGTEFKLPPLQVDVRLAKAGDRAVHDPTQILQTARGIEAGHIFQLGTKYSEAMGATFTDENGKEHPLVMGCYGVGVSRLA
QAAVEQSYDENGIIWPVAIAPYHAVVVVPNVKSEEQMAAAEKLYADLNAAGVETILDDRNERAGVKFKDAELIGIPFRVV
TGKSLKDGKVEVVRRKEGDRQDLDLDAVVATLKNWVEAAGQ

Sequences:

>Translated_601_residues
MRLSQSLFVTLREDPADAEIPSHKLLLRAGYIRRIGRGIYAYLPLMWRVLQKVSQIVREEMNATGAQETLLPQLQPAEIW
QESGRWDTYTQAEGIMFSLRDRLDGELGLGPTHEEVITTIAKDMIRSYRQLPQHLYQIQTKFRDEIRPRFGLMRGREFIM
KDGYSFHASEECLKKTYADMDQAYRNMLRRCGLQFRAVEADSGAIGGSGSQEFMILADAGEDEILYTEDEKYAANTEKAV
SLPVEAIASPFNSFEKRETPNTATIESLCKFLNCSPTCVVKNVLYQVVYNNGKTVLALISIRGDQDVNEVKLQNELTKLA
PNYDAKTVISLTVPDADAQQKWAAKSLPLGYISPALADDCIAKNKAVSGEFLRLVDPTAATLENFVTGADETNYHVLGAN
WGTEFKLPPLQVDVRLAKAGDRAVHDPTQILQTARGIEAGHIFQLGTKYSEAMGATFTDENGKEHPLVMGCYGVGVSRLA
QAAVEQSYDENGIIWPVAIAPYHAVVVVPNVKSEEQMAAAEKLYADLNAAGVETILDDRNERAGVKFKDAELIGIPFRVV
TGKSLKDGKVEVVRRKEGDRQDLDLDAVVATLKNWVEAAGQ
>Mature_601_residues
MRLSQSLFVTLREDPADAEIPSHKLLLRAGYIRRIGRGIYAYLPLMWRVLQKVSQIVREEMNATGAQETLLPQLQPAEIW
QESGRWDTYTQAEGIMFSLRDRLDGELGLGPTHEEVITTIAKDMIRSYRQLPQHLYQIQTKFRDEIRPRFGLMRGREFIM
KDGYSFHASEECLKKTYADMDQAYRNMLRRCGLQFRAVEADSGAIGGSGSQEFMILADAGEDEILYTEDEKYAANTEKAV
SLPVEAIASPFNSFEKRETPNTATIESLCKFLNCSPTCVVKNVLYQVVYNNGKTVLALISIRGDQDVNEVKLQNELTKLA
PNYDAKTVISLTVPDADAQQKWAAKSLPLGYISPALADDCIAKNKAVSGEFLRLVDPTAATLENFVTGADETNYHVLGAN
WGTEFKLPPLQVDVRLAKAGDRAVHDPTQILQTARGIEAGHIFQLGTKYSEAMGATFTDENGKEHPLVMGCYGVGVSRLA
QAAVEQSYDENGIIWPVAIAPYHAVVVVPNVKSEEQMAAAEKLYADLNAAGVETILDDRNERAGVKFKDAELIGIPFRVV
TGKSLKDGKVEVVRRKEGDRQDLDLDAVVATLKNWVEAAGQ

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=236, Percent_Identity=42.3728813559322, Blast_Score=192, Evalue=1e-48,
Organism=Escherichia coli, GI1786392, Length=595, Percent_Identity=44.0336134453782, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=35.3174603174603, Blast_Score=139, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320931, Length=205, Percent_Identity=38.0487804878049, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24656200, Length=217, Percent_Identity=38.2488479262673, Blast_Score=159, Evalue=7e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_SYNP2 (B1XP53)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734902.1
- ProteinModelPortal:   B1XP53
- SMR:   B1XP53
- GeneID:   6056549
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1656
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66718; Mature: 66718

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQSLFVTLREDPADAEIPSHKLLLRAGYIRRIGRGIYAYLPLMWRVLQKVSQIVREE
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNATGAQETLLPQLQPAEIWQESGRWDTYTQAEGIMFSLRDRLDGELGLGPTHEEVITTI
HCCCCCHHHHCCCCCHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
AKDMIRSYRQLPQHLYQIQTKFRDEIRPRFGLMRGREFIMKDGYSFHASEECLKKTYADM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHH
DQAYRNMLRRCGLQFRAVEADSGAIGGSGSQEFMILADAGEDEILYTEDEKYAANTEKAV
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHCCCCHHH
SLPVEAIASPFNSFEKRETPNTATIESLCKFLNCSPTCVVKNVLYQVVYNNGKTVLALIS
HCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEE
IRGDQDVNEVKLQNELTKLAPNYDAKTVISLTVPDADAQQKWAAKSLPLGYISPALADDC
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHCCHHHHHHH
IAKNKAVSGEFLRLVDPTAATLENFVTGADETNYHVLGANWGTEFKLPPLQVDVRLAKAG
HHCCCCCCCCHHEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCC
DRAVHDPTQILQTARGIEAGHIFQLGTKYSEAMGATFTDENGKEHPLVMGCYGVGVSRLA
CCCCCCHHHHHHHHCCCCCCCEEECCCHHHHHHCCEECCCCCCCCCEEEEEHHHCHHHHH
QAAVEQSYDENGIIWPVAIAPYHAVVVVPNVKSEEQMAAAEKLYADLNAAGVETILDDRN
HHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCC
ERAGVKFKDAELIGIPFRVVTGKSLKDGKVEVVRRKEGDRQDLDLDAVVATLKNWVEAAG
CCCCCEEECCEEEECCEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
Q
C
>Mature Secondary Structure
MRLSQSLFVTLREDPADAEIPSHKLLLRAGYIRRIGRGIYAYLPLMWRVLQKVSQIVREE
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNATGAQETLLPQLQPAEIWQESGRWDTYTQAEGIMFSLRDRLDGELGLGPTHEEVITTI
HCCCCCHHHHCCCCCHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
AKDMIRSYRQLPQHLYQIQTKFRDEIRPRFGLMRGREFIMKDGYSFHASEECLKKTYADM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHH
DQAYRNMLRRCGLQFRAVEADSGAIGGSGSQEFMILADAGEDEILYTEDEKYAANTEKAV
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHCCCCHHH
SLPVEAIASPFNSFEKRETPNTATIESLCKFLNCSPTCVVKNVLYQVVYNNGKTVLALIS
HCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEE
IRGDQDVNEVKLQNELTKLAPNYDAKTVISLTVPDADAQQKWAAKSLPLGYISPALADDC
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHCCHHHHHHH
IAKNKAVSGEFLRLVDPTAATLENFVTGADETNYHVLGANWGTEFKLPPLQVDVRLAKAG
HHCCCCCCCCHHEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCC
DRAVHDPTQILQTARGIEAGHIFQLGTKYSEAMGATFTDENGKEHPLVMGCYGVGVSRLA
CCCCCCHHHHHHHHCCCCCCCEEECCCHHHHHHCCEECCCCCCCCCEEEEEHHHCHHHHH
QAAVEQSYDENGIIWPVAIAPYHAVVVVPNVKSEEQMAAAEKLYADLNAAGVETILDDRN
HHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCC
ERAGVKFKDAELIGIPFRVVTGKSLKDGKVEVVRRKEGDRQDLDLDAVVATLKNWVEAAG
CCCCCEEECCEEEECCEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA