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The map label for this gene is nfi [H]

Identifier: 170078188

GI number: 170078188

Start: 1662065

End: 1662745

Strand: Reverse

Name: nfi [H]

Synonym: SYNPCC7002_A1579

Alternate gene names: 170078188

Gene position: 1662745-1662065 (Counterclockwise)

Preceding gene: 170078194

Following gene: 170078185

Centisome position: 55.28

GC content: 51.54

Gene sequence:

>681_bases
ATGCTTGTTTTACACCACAGTCATCCTTGGGATGTTTCTCCTGGGGTGGCCCGGGAGATTCAAACGTCTCTAGGGCAATG
GATCGTGCTTCAAGATGACTTACCAACAAACGTCAAAACCATTGCGGGGGTGGATGTGGGCTTTGAGGCGCGCTTCTCAA
TCACACGGGCGGCGGTGGTGGTTTTGTCCTTTCCCGATCTAGAGATCCTCGAAACGGCGATCGCCCGCTGTCCCACTTCT
TTTCCCTATGTACCGGGATTGTTATCTTTTCGGGAAGTGCCCGCCATCTTGGAGGCATTAGAAAAAATTACGCATTTACC
AGATTTATTGTTCTGTGACGGCCAAGGTTACGCCCATCCACGCCGTTTAGGGCTAGCCTCTCACTTGGGGGTCTTGTTAG
ATTTACCGACGATTGGGGTGGCAAAATCTCGCTACATCGGTACCCACCAAGAGGTGCCCCTCGAAAAAGGGCAGTGGGTG
CCTTTACAGGATCACGATGAAACTATTGGTGCAGTGCTCCGGAGTCGGACGAAGGTGCGCCCCCTTTATATTTCTGTGGG
TCATCGGATCAGTTTAGCCACGGCCCTTGATTTTGTTCTCGCCTGTACACCGAAATACCGTCTGCCAGAACCAACGCGCT
TGGCGGATAAATTGGCTTCTCGAAAACCTAATTTCCCATAA

Upstream 100 bases:

>100_bases
GATTGATTCTCAATTAATTGGTGAAATTTAACGGTTACTTGTTAATTGTGCCAAAACTTTTCCCGTAAACCCCTCGAAAT
GCAGATGAGGAATTATATAA

Downstream 100 bases:

>100_bases
AAATACCGCCTGGTGGCGGCATCGTTTTACTTAAAAAGTCAATTGATTGTTTAGGCGATCGCACAGGCACGACGGTAGCA
CCACCGCAGCAAAGTGATCC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MLVLHHSHPWDVSPGVAREIQTSLGQWIVLQDDLPTNVKTIAGVDVGFEARFSITRAAVVVLSFPDLEILETAIARCPTS
FPYVPGLLSFREVPAILEALEKITHLPDLLFCDGQGYAHPRRLGLASHLGVLLDLPTIGVAKSRYIGTHQEVPLEKGQWV
PLQDHDETIGAVLRSRTKVRPLYISVGHRISLATALDFVLACTPKYRLPEPTRLADKLASRKPNFP

Sequences:

>Translated_226_residues
MLVLHHSHPWDVSPGVAREIQTSLGQWIVLQDDLPTNVKTIAGVDVGFEARFSITRAAVVVLSFPDLEILETAIARCPTS
FPYVPGLLSFREVPAILEALEKITHLPDLLFCDGQGYAHPRRLGLASHLGVLLDLPTIGVAKSRYIGTHQEVPLEKGQWV
PLQDHDETIGAVLRSRTKVRPLYISVGHRISLATALDFVLACTPKYRLPEPTRLADKLASRKPNFP
>Mature_226_residues
MLVLHHSHPWDVSPGVAREIQTSLGQWIVLQDDLPTNVKTIAGVDVGFEARFSITRAAVVVLSFPDLEILETAIARCPTS
FPYVPGLLSFREVPAILEALEKITHLPDLLFCDGQGYAHPRRLGLASHLGVLLDLPTIGVAKSRYIGTHQEVPLEKGQWV
PLQDHDETIGAVLRSRTKVRPLYISVGHRISLATALDFVLACTPKYRLPEPTRLADKLASRKPNFP

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=205, Percent_Identity=39.5121951219512, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI257467548, Length=200, Percent_Identity=34, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI257467550, Length=200, Percent_Identity=34, Blast_Score=85, Evalue=5e-17,
Organism=Escherichia coli, GI87082357, Length=209, Percent_Identity=48.8038277511962, Blast_Score=206, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI71981509, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 24972; Mature: 24972

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLHHSHPWDVSPGVAREIQTSLGQWIVLQDDLPTNVKTIAGVDVGFEARFSITRAAVV
CEEEECCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCHHHHHHHEEEEE
VLSFPDLEILETAIARCPTSFPYVPGLLSFREVPAILEALEKITHLPDLLFCDGQGYAHP
EEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
RRLGLASHLGVLLDLPTIGVAKSRYIGTHQEVPLEKGQWVPLQDHDETIGAVLRSRTKVR
HHHHHHHHHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PLYISVGHRISLATALDFVLACTPKYRLPEPTRLADKLASRKPNFP
EEEEECCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLVLHHSHPWDVSPGVAREIQTSLGQWIVLQDDLPTNVKTIAGVDVGFEARFSITRAAVV
CEEEECCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCHHHHHHHEEEEE
VLSFPDLEILETAIARCPTSFPYVPGLLSFREVPAILEALEKITHLPDLLFCDGQGYAHP
EEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
RRLGLASHLGVLLDLPTIGVAKSRYIGTHQEVPLEKGQWVPLQDHDETIGAVLRSRTKVR
HHHHHHHHHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PLYISVGHRISLATALDFVLACTPKYRLPEPTRLADKLASRKPNFP
EEEEECCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA