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The map label for this gene is leuA

Identifier: 170077970

GI number: 170077970

Start: 1416090

End: 1417691

Strand: Reverse

Name: leuA

Synonym: SYNPCC7002_A1356

Alternate gene names: 170077970

Gene position: 1417691-1416090 (Counterclockwise)

Preceding gene: 170077971

Following gene: 170077968

Centisome position: 47.13

GC content: 51.19

Gene sequence:

>1602_bases
ATGAACAACCAAGACGATCGCATTATTATTTTCGACACAACATTACGGGATGGTGAACAATCTCCGGGGGCAACCCTAAA
TGGAGACGAAAAGCTGGCGATCGCCCGTGCCCTGGCGCGACTTGGTGTAGATGTCATTGAAGCGGGTTTCCCCAGGGCGA
GTCGGGGTGATTTTGATGCTGTGCAACGTATCGCCGCCGAAGTCGGCACAGAAAATGGGCCAACGATCTGTGGTCTAGCC
CGGGCCACCAAGGGCGACATTGAAGCGGCAGGCAATGCCCTGAAGCCAGCTTTTAGCAATCGAATCCACACCTTTATCGC
CACCTCCGATATTCACCTCGAGTACAAACTCCGCAAAAGCCGCAAGGATGTGTTGGCGATCGCCCCGGAAATGGTTGCCT
ACGCCAAAACCTTTACCAACGATGTGGAATTTTCCCCCGAAGACGCAGGCCGCTCGGATCCTGAATTCCTTTATCAAATC
CTCGAAGCCGTCATTGATGCCGGGGCAACCACCGTTAATATTCCTGACACCGTCGGCTACACAACCCCCGCCGAATTTGG
CGCTCTGATCAAAGGCATCAAAGAAAACGTCCCCAATATTGACCGCGCAATTATCTCTGTCCATGGCCACAACGATTTGG
GCCTAGCGGTGGCCAATTTCCTCGAAGCCGTAAAAAATGGCGCTCGGCAGTTGGAATGCACTATTAATGGCATCGGCGAA
CGGGCCGGGAATGCCGCCCTCGAAGAATTGGTGATGGCTCTCCATGTGCGCCGCCAGTATTACAATCCTTTCCTTGGTCG
GCCTGCTGACTCAGAAGCACCTTTGACCAGCATCAATACCAAAGAGATCTACAAAACATCTCGCTTGGTATCTAACTTGA
CGGGGCTTTCTGTCCAGGCCAATAAAGCGATTGTGGGTTTGAATGCCTTTGCCCACGAGTCAGGCATTCACCAGGATGGC
GTCCTGAAAAATAAGCTCACCTACGAGATCATGGATGCCCAGTCCATTGGCTTAACTGATAATCAAATTATCTTGGGTAA
ACATTCTGGTCGGAATGCTTTCCGTACCCGCTTGGCAGAATTAGGGTTCGATCTGTCTGACAACGATCTCAACAAAGCTT
TTCTGCGCTTTAAAGATCTGGCCGATAAGAAAAAAGAAATTACCGACTGGGATCTTGAGGCGATCGTTAAGGATGAAACC
CAGCAACCGCCGGAATTATTCCGTCTGGAGCTGGTGCAGGTTTCCTGTGGTGATCATGCTCAACCCACAGCCACCATTAC
GATCCGTACTCCCGGAGGAAAAGAATTAACCGATGCAGCCATTGGTACTGGGCCTGTAGATGCCATTTATAAGGCGATTA
ACCGGGTCGTTCAGGTGCCCAATGAATTAATTGAATATTCCGTGCAGTCGGTAACGGCGGGCATTGATGCCATTGGTGAA
GTCACGATTCGTTTGCGGCACGAGGGACGGATTTATTCTGGCCATGCTGCAAATACAGATATTGTGGTAGCGTCGGCCCG
GGCGTACATCAGCGCCTTGAATCGTTTATATGCCGCTCTTCAGGAAGATGTGTCGGCTAATCCAGCGAAGGCTTCTCTGT
AG

Upstream 100 bases:

>100_bases
AGCTATGGACTCTTTTAAAGTGGAGCATAGCCAGTACTTTTATTCCAATCAACATAGAGTTTTTATTTAGACTATTTTCA
CGCTAAGGGAGCGGGAAATG

Downstream 100 bases:

>100_bases
TTGCTGCGACAGTTAACCCGCGCGCTTTTTCCCAGCTTTTTTTCAAGGGGACTTTTTCTCTAATTAATTGGGGAAAAGTC
TCTTTTTTTATGTGTGGGAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MNNQDDRIIIFDTTLRDGEQSPGATLNGDEKLAIARALARLGVDVIEAGFPRASRGDFDAVQRIAAEVGTENGPTICGLA
RATKGDIEAAGNALKPAFSNRIHTFIATSDIHLEYKLRKSRKDVLAIAPEMVAYAKTFTNDVEFSPEDAGRSDPEFLYQI
LEAVIDAGATTVNIPDTVGYTTPAEFGALIKGIKENVPNIDRAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMALHVRRQYYNPFLGRPADSEAPLTSINTKEIYKTSRLVSNLTGLSVQANKAIVGLNAFAHESGIHQDG
VLKNKLTYEIMDAQSIGLTDNQIILGKHSGRNAFRTRLAELGFDLSDNDLNKAFLRFKDLADKKKEITDWDLEAIVKDET
QQPPELFRLELVQVSCGDHAQPTATITIRTPGGKELTDAAIGTGPVDAIYKAINRVVQVPNELIEYSVQSVTAGIDAIGE
VTIRLRHEGRIYSGHAANTDIVVASARAYISALNRLYAALQEDVSANPAKASL

Sequences:

>Translated_533_residues
MNNQDDRIIIFDTTLRDGEQSPGATLNGDEKLAIARALARLGVDVIEAGFPRASRGDFDAVQRIAAEVGTENGPTICGLA
RATKGDIEAAGNALKPAFSNRIHTFIATSDIHLEYKLRKSRKDVLAIAPEMVAYAKTFTNDVEFSPEDAGRSDPEFLYQI
LEAVIDAGATTVNIPDTVGYTTPAEFGALIKGIKENVPNIDRAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMALHVRRQYYNPFLGRPADSEAPLTSINTKEIYKTSRLVSNLTGLSVQANKAIVGLNAFAHESGIHQDG
VLKNKLTYEIMDAQSIGLTDNQIILGKHSGRNAFRTRLAELGFDLSDNDLNKAFLRFKDLADKKKEITDWDLEAIVKDET
QQPPELFRLELVQVSCGDHAQPTATITIRTPGGKELTDAAIGTGPVDAIYKAINRVVQVPNELIEYSVQSVTAGIDAIGE
VTIRLRHEGRIYSGHAANTDIVVASARAYISALNRLYAALQEDVSANPAKASL
>Mature_533_residues
MNNQDDRIIIFDTTLRDGEQSPGATLNGDEKLAIARALARLGVDVIEAGFPRASRGDFDAVQRIAAEVGTENGPTICGLA
RATKGDIEAAGNALKPAFSNRIHTFIATSDIHLEYKLRKSRKDVLAIAPEMVAYAKTFTNDVEFSPEDAGRSDPEFLYQI
LEAVIDAGATTVNIPDTVGYTTPAEFGALIKGIKENVPNIDRAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMALHVRRQYYNPFLGRPADSEAPLTSINTKEIYKTSRLVSNLTGLSVQANKAIVGLNAFAHESGIHQDG
VLKNKLTYEIMDAQSIGLTDNQIILGKHSGRNAFRTRLAELGFDLSDNDLNKAFLRFKDLADKKKEITDWDLEAIVKDET
QQPPELFRLELVQVSCGDHAQPTATITIRTPGGKELTDAAIGTGPVDAIYKAINRVVQVPNELIEYSVQSVTAGIDAIGE
VTIRLRHEGRIYSGHAANTDIVVASARAYISALNRLYAALQEDVSANPAKASL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=515, Percent_Identity=49.7087378640777, Blast_Score=471, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6320071, Length=401, Percent_Identity=29.1770573566085, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320019, Length=394, Percent_Identity=30.4568527918782, Blast_Score=150, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6324225, Length=558, Percent_Identity=25.6272401433692, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324682, Length=332, Percent_Identity=29.2168674698795, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_SYNP2 (B1XLQ9)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734608.1
- ProteinModelPortal:   B1XLQ9
- SMR:   B1XLQ9
- GeneID:   6055023
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1356
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57651; Mature: 57651

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQDDRIIIFDTTLRDGEQSPGATLNGDEKLAIARALARLGVDVIEAGFPRASRGDFDA
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VQRIAAEVGTENGPTICGLARATKGDIEAAGNALKPAFSNRIHTFIATSDIHLEYKLRKS
HHHHHHHHCCCCCCEEEEECCCCCCCHHHCCCCCCHHHHCCEEEEEEECEEEEEEEEHHC
RKDVLAIAPEMVAYAKTFTNDVEFSPEDAGRSDPEFLYQILEAVIDAGATTVNIPDTVGY
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
TTPAEFGALIKGIKENVPNIDRAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECHHH
RAGNAALEELVMALHVRRQYYNPFLGRPADSEAPLTSINTKEIYKTSRLVSNLTGLSVQA
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEC
NKAIVGLNAFAHESGIHQDGVLKNKLTYEIMDAQSIGLTDNQIILGKHSGRNAFRTRLAE
CCEEEEEHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHH
LGFDLSDNDLNKAFLRFKDLADKKKEITDWDLEAIVKDETQQPPELFRLELVQVSCGDHA
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHEEEEEEECCCCCC
QPTATITIRTPGGKELTDAAIGTGPVDAIYKAINRVVQVPNELIEYSVQSVTAGIDAIGE
CCEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCE
VTIRLRHEGRIYSGHAANTDIVVASARAYISALNRLYAALQEDVSANPAKASL
EEEEEEECCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNNQDDRIIIFDTTLRDGEQSPGATLNGDEKLAIARALARLGVDVIEAGFPRASRGDFDA
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VQRIAAEVGTENGPTICGLARATKGDIEAAGNALKPAFSNRIHTFIATSDIHLEYKLRKS
HHHHHHHHCCCCCCEEEEECCCCCCCHHHCCCCCCHHHHCCEEEEEEECEEEEEEEEHHC
RKDVLAIAPEMVAYAKTFTNDVEFSPEDAGRSDPEFLYQILEAVIDAGATTVNIPDTVGY
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
TTPAEFGALIKGIKENVPNIDRAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECHHH
RAGNAALEELVMALHVRRQYYNPFLGRPADSEAPLTSINTKEIYKTSRLVSNLTGLSVQA
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEC
NKAIVGLNAFAHESGIHQDGVLKNKLTYEIMDAQSIGLTDNQIILGKHSGRNAFRTRLAE
CCEEEEEHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHH
LGFDLSDNDLNKAFLRFKDLADKKKEITDWDLEAIVKDETQQPPELFRLELVQVSCGDHA
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHEEEEEEECCCCCC
QPTATITIRTPGGKELTDAAIGTGPVDAIYKAINRVVQVPNELIEYSVQSVTAGIDAIGE
CCEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCE
VTIRLRHEGRIYSGHAANTDIVVASARAYISALNRLYAALQEDVSANPAKASL
EEEEEEECCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA