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The map label for this gene is clpP [H]

Identifier: 170077810

GI number: 170077810

Start: 1238089

End: 1238775

Strand: Direct

Name: clpP [H]

Synonym: SYNPCC7002_A1193

Alternate gene names: 170077810

Gene position: 1238089-1238775 (Clockwise)

Preceding gene: 170077808

Following gene: 170077811

Centisome position: 41.16

GC content: 51.38

Gene sequence:

>687_bases
ATGAACTCACCGATTCAGGCTGTACAAGCACCCTACAGAGGTGGCGGCTCCACCTACAGGACGCCACCGCCAGATCTCCC
TTCCTTACTGTTAAAAGAACGCATCGTTTATCTCGGCCTTCCCCTCGTCTCTCCCGATGAGTATAAAGATCAAATTGGCG
TTGATGTGACCGAGCTGATCATCGCCCAACTGTTATTTTTACAGTTTGATGATCCCGATAAACCGATTTATATGTACATC
AACTCCACCGGGACTTCTTGGTACGGTGGTGATTCCATTGGGTTTGAAACAGAAGCCTTTGCCATCTGCGATACCCTCAA
CTACATCACGCCGCCAGTGCATACCATTTGTCTCGGTCAAGCGATGGGGACAGCGGCCATGATCCTTGCTGCTGGTACGA
AGGGCTGCCGCGCGAGCCTGCCCCACTCGACGATTATTCTCCACCAAGCACGCCAAGGGGCCCAGGGCCAAGCCTCCGAT
ATTCAAATTCGGGCGAAGGAAGTGATCGAAAACAAACGGACAATCCTGCAAATGATGTCTAAATATACAGGTCAACCCCT
CGAAAAACTCGAAAAAGACACTGACCGGATGTTTTACATGACACCCCAGCAGGCCCTCGAATACGGCATTATCGATAAAG
TGCTCGAAAGTTCGAAGGACTTGCCGTCCCCCGTACCGGCTCTGTAG

Upstream 100 bases:

>100_bases
CCGTCAAACCTTGCGTTAAAATTAGATGCTAAGACAAAATCAGACAAAAACACCCCGCAACCGTAACGCCAACACGTTAC
CCAACGCATTAAAAAGATCT

Downstream 100 bases:

>100_bases
TCTTCGTGATTGCTTAAAATCACCCCATTTTTTCCCGTTGTTCAGATCAAGGTAAACAAAATCCATGCCCATCGGTGTAC
CCAAAGTTCCCTATCAAATG

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNSPIQAVQAPYRGGGSTYRTPPPDLPSLLLKERIVYLGLPLVSPDEYKDQIGVDVTELIIAQLLFLQFDDPDKPIYMYI
NSTGTSWYGGDSIGFETEAFAICDTLNYITPPVHTICLGQAMGTAAMILAAGTKGCRASLPHSTIILHQARQGAQGQASD
IQIRAKEVIENKRTILQMMSKYTGQPLEKLEKDTDRMFYMTPQQALEYGIIDKVLESSKDLPSPVPAL

Sequences:

>Translated_228_residues
MNSPIQAVQAPYRGGGSTYRTPPPDLPSLLLKERIVYLGLPLVSPDEYKDQIGVDVTELIIAQLLFLQFDDPDKPIYMYI
NSTGTSWYGGDSIGFETEAFAICDTLNYITPPVHTICLGQAMGTAAMILAAGTKGCRASLPHSTIILHQARQGAQGQASD
IQIRAKEVIENKRTILQMMSKYTGQPLEKLEKDTDRMFYMTPQQALEYGIIDKVLESSKDLPSPVPAL
>Mature_228_residues
MNSPIQAVQAPYRGGGSTYRTPPPDLPSLLLKERIVYLGLPLVSPDEYKDQIGVDVTELIIAQLLFLQFDDPDKPIYMYI
NSTGTSWYGGDSIGFETEAFAICDTLNYITPPVHTICLGQAMGTAAMILAAGTKGCRASLPHSTIILHQARQGAQGQASD
IQIRAKEVIENKRTILQMMSKYTGQPLEKLEKDTDRMFYMTPQQALEYGIIDKVLESSKDLPSPVPAL

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=191, Percent_Identity=44.5026178010471, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1786641, Length=191, Percent_Identity=40.8376963350785, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17538017, Length=199, Percent_Identity=39.1959798994975, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI20129427, Length=192, Percent_Identity=41.6666666666667, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 25096; Mature: 25096

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPIQAVQAPYRGGGSTYRTPPPDLPSLLLKERIVYLGLPLVSPDEYKDQIGVDVTELI
CCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHH
IAQLLFLQFDDPDKPIYMYINSTGTSWYGGDSIGFETEAFAICDTLNYITPPVHTICLGQ
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
AMGTAAMILAAGTKGCRASLPHSTIILHQARQGAQGQASDIQIRAKEVIENKRTILQMMS
HHHHHHHHHHCCCCCCCCCCCCHHEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KYTGQPLEKLEKDTDRMFYMTPQQALEYGIIDKVLESSKDLPSPVPAL
HHCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNSPIQAVQAPYRGGGSTYRTPPPDLPSLLLKERIVYLGLPLVSPDEYKDQIGVDVTELI
CCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHH
IAQLLFLQFDDPDKPIYMYINSTGTSWYGGDSIGFETEAFAICDTLNYITPPVHTICLGQ
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
AMGTAAMILAAGTKGCRASLPHSTIILHQARQGAQGQASDIQIRAKEVIENKRTILQMMS
HHHHHHHHHHCCCCCCCCCCCCHHEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KYTGQPLEKLEKDTDRMFYMTPQQALEYGIIDKVLESSKDLPSPVPAL
HHCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 0.21 {ATP}} 1.3 {N-succinyl-Leu-Tyr} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]