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The map label for this gene is cysG/hemD [H]

Identifier: 170077809

GI number: 170077809

Start: 1236479

End: 1237969

Strand: Reverse

Name: cysG/hemD [H]

Synonym: SYNPCC7002_A1192

Alternate gene names: 170077809

Gene position: 1237969-1236479 (Counterclockwise)

Preceding gene: 170077812

Following gene: 170077804

Centisome position: 41.16

GC content: 51.71

Gene sequence:

>1491_bases
ATGGGAAAAGTATTTTTGCTAGGAGCGGGTTTAGGGACAAAGAACTATTTAACGGTGCGAGGTTGGGAGATCCTCCAACG
GGCGGAAGTGGTGATTTACGACGCCCTGGGTTCCCAAGAACTGTTGGATCTGCTGCCCCAGGACTGTTTACGGTTATTCG
TCGGTAAGCGAGGCGGCCAAAAGAGTACGCCCCAAGAAGAAATTAATCGCCTTTTGGTTGAGCATGGACAATCGCCAAAA
CGGGTGGTGCGCCTGAAGGGGGGAGATCCGTATATTTTTGGGCGATCGCACCCAGAGATTTTGGCACTCCAAGGGGCGGG
CTGTGATTTTGAAGTGATTCCTGGCATTTCATCGGCCTTCGCAGCACCTTTGCTAGCGGGAATTCCCCTTACCCACAAGG
ATTTGGGGCGCGGCTTTTTTGTAGGGACAGCCCATGATCTAGAGGCCTTTGATTGGACAGCCCTAGCCCAATTGGAAACT
TTGGTTTTTTTGATGGGGGGACGCAATTTAGAAGACATTTGTCGGCGCTTGATGGCAGCGGGGCGATCGCCTCATTTACC
AGTGGCCATTGTGCGGGCGGCGGGATTACCAGAGCAGCAGGTCTGGACGGGAACGTTACAGACCATTACGAAGCAAACGG
CGACGGTTCAAAAACTTTCGCCCTGTGTGATCATTGTGGGTCAGGTAGTTAGTCTCCGGGAGGATTACATGGATCGGGCT
CCATTGGGTGGCAAAACAATTCTGATTACCCGCGCTGCGTCGCAATCGAGCGAATTTACCAAACTTTTGGCAGAAACTGG
CGCAAAGGTCTTGGAAATGCCCGCTTTAGAGATTCGTGAGCCCTCCACCTGGGAACCCCTCGACCAGGCGATCGCCACCC
TAGAAACCTTTGATTGGCTGATTCTCACCTCGGCCAATGGCGTTGAATATTTTTTCCAGCGACTCGAAACCTTGGGCAAA
GATGTCCGCGCCTTAGGCAAGCTCAAAATTGCCGTCGTCGGCAAAAAAACAGAAAAATTCTTGCGCCAGCACGGCCTCAA
AGCGGACTATATTCCCCCCGATTTTATTGCAGATTCCTTAGTGGAAAATTTCCCAGATCCTCTGCAAAATCAAAAAATTC
TTTTTCCCCGGGTCGAAACGGGAGGCCGAAAAATCCTGGTGCAAGAGCTCGCCCAACAGGGTGCCCAGGTGGTTGAAGTG
CCCGCCTATGAATCGGGCTGTCCGCGACAAATTCCGGCGGCGGTCTGGCAAGCTCTCGAAGCACAAACCATTGATCTAGT
AACCTTTGCGAGCTCTAAAACAGTGCGCAACTTTTGGCAGCTAATTCAGCAGCAGGGGGGGGATTTAGCGTTATTAAAAC
CGCTGAAAATTGCCTCCATTGGCCCCCAAACTTCCCAGACTTGTTTAAACCTTTTCCAGCGGGTTGATCTCGAAGCCGAG
GTCTACACCCTAGAAGGCTTACGGGATGTCATTCTGGACAATTGCGTATAG

Upstream 100 bases:

>100_bases
GGTAACGTGTTGGCGTTACGGTTGCGGGGTGTTTTTGTCTGATTTTGTCTTAGCATCTAATTTTAACGCAAGGTTTGACG
GTGGTTTAGGGCGATCGCAC

Downstream 100 bases:

>100_bases
TTCACAATCCCGAAGAGCGGACTTGATAGGGGATTTCTTCGATGGGGAGATTGTCTAAATTCCAGTCGGCGTAATGCAAT
TGGAGGCGTTTTTCGCCCGC

Product: uroporphyrin-III synthase/methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MGKVFLLGAGLGTKNYLTVRGWEILQRAEVVIYDALGSQELLDLLPQDCLRLFVGKRGGQKSTPQEEINRLLVEHGQSPK
RVVRLKGGDPYIFGRSHPEILALQGAGCDFEVIPGISSAFAAPLLAGIPLTHKDLGRGFFVGTAHDLEAFDWTALAQLET
LVFLMGGRNLEDICRRLMAAGRSPHLPVAIVRAAGLPEQQVWTGTLQTITKQTATVQKLSPCVIIVGQVVSLREDYMDRA
PLGGKTILITRAASQSSEFTKLLAETGAKVLEMPALEIREPSTWEPLDQAIATLETFDWLILTSANGVEYFFQRLETLGK
DVRALGKLKIAVVGKKTEKFLRQHGLKADYIPPDFIADSLVENFPDPLQNQKILFPRVETGGRKILVQELAQQGAQVVEV
PAYESGCPRQIPAAVWQALEAQTIDLVTFASSKTVRNFWQLIQQQGGDLALLKPLKIASIGPQTSQTCLNLFQRVDLEAE
VYTLEGLRDVILDNCV

Sequences:

>Translated_496_residues
MGKVFLLGAGLGTKNYLTVRGWEILQRAEVVIYDALGSQELLDLLPQDCLRLFVGKRGGQKSTPQEEINRLLVEHGQSPK
RVVRLKGGDPYIFGRSHPEILALQGAGCDFEVIPGISSAFAAPLLAGIPLTHKDLGRGFFVGTAHDLEAFDWTALAQLET
LVFLMGGRNLEDICRRLMAAGRSPHLPVAIVRAAGLPEQQVWTGTLQTITKQTATVQKLSPCVIIVGQVVSLREDYMDRA
PLGGKTILITRAASQSSEFTKLLAETGAKVLEMPALEIREPSTWEPLDQAIATLETFDWLILTSANGVEYFFQRLETLGK
DVRALGKLKIAVVGKKTEKFLRQHGLKADYIPPDFIADSLVENFPDPLQNQKILFPRVETGGRKILVQELAQQGAQVVEV
PAYESGCPRQIPAAVWQALEAQTIDLVTFASSKTVRNFWQLIQQQGGDLALLKPLKIASIGPQTSQTCLNLFQRVDLEAE
VYTLEGLRDVILDNCV
>Mature_495_residues
GKVFLLGAGLGTKNYLTVRGWEILQRAEVVIYDALGSQELLDLLPQDCLRLFVGKRGGQKSTPQEEINRLLVEHGQSPKR
VVRLKGGDPYIFGRSHPEILALQGAGCDFEVIPGISSAFAAPLLAGIPLTHKDLGRGFFVGTAHDLEAFDWTALAQLETL
VFLMGGRNLEDICRRLMAAGRSPHLPVAIVRAAGLPEQQVWTGTLQTITKQTATVQKLSPCVIIVGQVVSLREDYMDRAP
LGGKTILITRAASQSSEFTKLLAETGAKVLEMPALEIREPSTWEPLDQAIATLETFDWLILTSANGVEYFFQRLETLGKD
VRALGKLKIAVVGKKTEKFLRQHGLKADYIPPDFIADSLVENFPDPLQNQKILFPRVETGGRKILVQELAQQGAQVVEVP
AYESGCPRQIPAAVWQALEAQTIDLVTFASSKTVRNFWQLIQQQGGDLALLKPLKIASIGPQTSQTCLNLFQRVDLEAEV
YTLEGLRDVILDNCV

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=236, Percent_Identity=43.2203389830509, Blast_Score=176, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=30.4721030042918, Blast_Score=103, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54511; Mature: 54380

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVFLLGAGLGTKNYLTVRGWEILQRAEVVIYDALGSQELLDLLPQDCLRLFVGKRGGQ
CCEEEEEECCCCCCCEEEECHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
KSTPQEEINRLLVEHGQSPKRVVRLKGGDPYIFGRSHPEILALQGAGCDFEVIPGISSAF
CCCCHHHHHHHHHHCCCCCHHEEEEECCCEEEECCCCCCEEEEECCCCCEEECCCCHHHH
AAPLLAGIPLTHKDLGRGFFVGTAHDLEAFDWTALAQLETLVFLMGGRNLEDICRRLMAA
HHHHHHCCCCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GRSPHLPVAIVRAAGLPEQQVWTGTLQTITKQTATVQKLSPCVIIVGQVVSLREDYMDRA
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
PLGGKTILITRAASQSSEFTKLLAETGAKVLEMPALEIREPSTWEPLDQAIATLETFDWL
CCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCEE
ILTSANGVEYFFQRLETLGKDVRALGKLKIAVVGKKTEKFLRQHGLKADYIPPDFIADSL
EEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHH
VENFPDPLQNQKILFPRVETGGRKILVQELAQQGAQVVEVPAYESGCPRQIPAAVWQALE
HHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHC
AQTIDLVTFASSKTVRNFWQLIQQQGGDLALLKPLKIASIGPQTSQTCLNLFQRVDLEAE
CCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCEEEECCCCCHHHHHHHHHHHCCCCEE
VYTLEGLRDVILDNCV
EEEHHHHHHHHHHCCC
>Mature Secondary Structure 
GKVFLLGAGLGTKNYLTVRGWEILQRAEVVIYDALGSQELLDLLPQDCLRLFVGKRGGQ
CEEEEEECCCCCCCEEEECHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
KSTPQEEINRLLVEHGQSPKRVVRLKGGDPYIFGRSHPEILALQGAGCDFEVIPGISSAF
CCCCHHHHHHHHHHCCCCCHHEEEEECCCEEEECCCCCCEEEEECCCCCEEECCCCHHHH
AAPLLAGIPLTHKDLGRGFFVGTAHDLEAFDWTALAQLETLVFLMGGRNLEDICRRLMAA
HHHHHHCCCCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GRSPHLPVAIVRAAGLPEQQVWTGTLQTITKQTATVQKLSPCVIIVGQVVSLREDYMDRA
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
PLGGKTILITRAASQSSEFTKLLAETGAKVLEMPALEIREPSTWEPLDQAIATLETFDWL
CCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCEE
ILTSANGVEYFFQRLETLGKDVRALGKLKIAVVGKKTEKFLRQHGLKADYIPPDFIADSL
EEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHH
VENFPDPLQNQKILFPRVETGGRKILVQELAQQGAQVVEVPAYESGCPRQIPAAVWQALE
HHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHC
AQTIDLVTFASSKTVRNFWQLIQQQGGDLALLKPLKIASIGPQTSQTCLNLFQRVDLEAE
CCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCEEEECCCCCHHHHHHHHHHHCCCCEE
VYTLEGLRDVILDNCV
EEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]