Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 170077765

GI number: 170077765

Start: 1180882

End: 1181841

Strand: Reverse

Name: gshB [H]

Synonym: SYNPCC7002_A1147

Alternate gene names: 170077765

Gene position: 1181841-1180882 (Counterclockwise)

Preceding gene: 170077766

Following gene: 170077762

Centisome position: 39.29

GC content: 53.65

Gene sequence:

>960_bases
GTGAAACTGGCCTTTATCCTCGATCCCATTGCCAACCTTGACCCCTGCCATGATACGACGGTGGCTTTTATGGAAGCGGC
CCAACTCCTCGGCCATGAGGTTTGGATTACGACCATGGAGCAGTTGAGTGTGGTTGACCACCAGGCCTGGGCAACGCTGC
AGGCAGTGCAATTAGAGCCAGTGCGTTTGGAAAATGGTCTCTGGGTTGCTGAGGAACATTGGTTTCGCCTCGGAGAACGG
AAATTGTTGCCCCTCACGGAGATGAATGCGGTCTTTATGCGCACCGATCCGCCTGTGACTTCGGCATATCTCTATGCCAC
CTACATTTTAGATTTGCTCATCCCCACCAAAACCCTGGTGTTGAATAATCCCCAGGGGATCCGCGCCGCCAACGAAAAAC
TCTACGCCCTCCGGTTTCCCACGGTGATTCCCGCCAGCATTATCACTACAGAAAAGGCAGTGGCACGACAATTTATCGAA
GCCAAAGGAAAAGCCGTTTTAAAGCCCCTCGGTGGGAAAGCAGGGGAAGGGATTTTGTTAATCAAAAAAGGCGATCGCAA
TTTTAATTCCCTCGTAGAAATCAGCACCAAACAGGGGAAAGAGCCGGTGATGGTGCAGGAATTTTTGCCCGCTGCCCAGG
CAGGCGATAAGCGTCTGATCGTCTTGGATGGAGACCCGATTGGGGCCGTAAACCGCATTCCGACGGGGGACGATTTCCGG
GGCAATATGGCCGTTGGTGGTCGCATCGCCCAGGTGGATATTACGGAGCGCGACCGGGAAATTGCCGCAACCTTAGCTCC
CCAACTCAGGGCCGATGGGTTATATTTTGTCGGCCTCGATATTATTGGTGGCTATGTGACGGAGGTGAATGTCACCAGTC
CCACAGGTGTCCGGGAAGCCGATCGCCTCGATGGGGTCTCCATCGGTAAACAGGTGATTGAATGGGTGGCAGATCGATAA

Upstream 100 bases:

>100_bases
TGAGGCGATCGCCCCCCTTGGGTAAAGCGAATCCTTTGCCTTTTCAATTGACGATTGATTATGATCAGTGGGTTCCATTG
TGTTTAGGAAGCAACCCCCT

Downstream 100 bases:

>100_bases
ACAGGTGATTGAATGGGTGGCAGAGCAGACATCCCCCTAAATTTCTAGCAGAGGGCGGGTGCTAAAACGTCGGTGATCAG
GTCGGCGCTGACTTGATCGG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLAFILDPIANLDPCHDTTVAFMEAAQLLGHEVWITTMEQLSVVDHQAWATLQAVQLEPVRLENGLWVAEEHWFRLGER
KLLPLTEMNAVFMRTDPPVTSAYLYATYILDLLIPTKTLVLNNPQGIRAANEKLYALRFPTVIPASIITTEKAVARQFIE
AKGKAVLKPLGGKAGEGILLIKKGDRNFNSLVEISTKQGKEPVMVQEFLPAAQAGDKRLIVLDGDPIGAVNRIPTGDDFR
GNMAVGGRIAQVDITERDREIAATLAPQLRADGLYFVGLDIIGGYVTEVNVTSPTGVREADRLDGVSIGKQVIEWVADR

Sequences:

>Translated_319_residues
MKLAFILDPIANLDPCHDTTVAFMEAAQLLGHEVWITTMEQLSVVDHQAWATLQAVQLEPVRLENGLWVAEEHWFRLGER
KLLPLTEMNAVFMRTDPPVTSAYLYATYILDLLIPTKTLVLNNPQGIRAANEKLYALRFPTVIPASIITTEKAVARQFIE
AKGKAVLKPLGGKAGEGILLIKKGDRNFNSLVEISTKQGKEPVMVQEFLPAAQAGDKRLIVLDGDPIGAVNRIPTGDDFR
GNMAVGGRIAQVDITERDREIAATLAPQLRADGLYFVGLDIIGGYVTEVNVTSPTGVREADRLDGVSIGKQVIEWVADR
>Mature_319_residues
MKLAFILDPIANLDPCHDTTVAFMEAAQLLGHEVWITTMEQLSVVDHQAWATLQAVQLEPVRLENGLWVAEEHWFRLGER
KLLPLTEMNAVFMRTDPPVTSAYLYATYILDLLIPTKTLVLNNPQGIRAANEKLYALRFPTVIPASIITTEKAVARQFIE
AKGKAVLKPLGGKAGEGILLIKKGDRNFNSLVEISTKQGKEPVMVQEFLPAAQAGDKRLIVLDGDPIGAVNRIPTGDDFR
GNMAVGGRIAQVDITERDREIAATLAPQLRADGLYFVGLDIIGGYVTEVNVTSPTGVREADRLDGVSIGKQVIEWVADR

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=320, Percent_Identity=37.5, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35018; Mature: 35018

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAFILDPIANLDPCHDTTVAFMEAAQLLGHEVWITTMEQLSVVDHQAWATLQAVQLEP
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHEEEEEEECC
VRLENGLWVAEEHWFRLGERKLLPLTEMNAVFMRTDPPVTSAYLYATYILDLLIPTKTLV
EEECCCEEEEHHHHHHCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEE
LNNPQGIRAANEKLYALRFPTVIPASIITTEKAVARQFIEAKGKAVLKPLGGKAGEGILL
EECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEE
IKKGDRNFNSLVEISTKQGKEPVMVQEFLPAAQAGDKRLIVLDGDPIGAVNRIPTGDDFR
EEECCCCCCCEEEEECCCCCCCCHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
GNMAVGGRIAQVDITERDREIAATLAPQLRADGLYFVGLDIIGGYVTEVNVTSPTGVREA
CCEEECCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEHHCCEEEEEEECCCCCCCHH
DRLDGVSIGKQVIEWVADR
HHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLAFILDPIANLDPCHDTTVAFMEAAQLLGHEVWITTMEQLSVVDHQAWATLQAVQLEP
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHEEEEEEECC
VRLENGLWVAEEHWFRLGERKLLPLTEMNAVFMRTDPPVTSAYLYATYILDLLIPTKTLV
EEECCCEEEEHHHHHHCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEE
LNNPQGIRAANEKLYALRFPTVIPASIITTEKAVARQFIEAKGKAVLKPLGGKAGEGILL
EECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEE
IKKGDRNFNSLVEISTKQGKEPVMVQEFLPAAQAGDKRLIVLDGDPIGAVNRIPTGDDFR
EEECCCCCCCEEEEECCCCCCCCHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
GNMAVGGRIAQVDITERDREIAATLAPQLRADGLYFVGLDIIGGYVTEVNVTSPTGVREA
CCEEECCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEHHCCEEEEEEECCCCCCCHH
DRLDGVSIGKQVIEWVADR
HHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]