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The map label for this gene is dus1 [H]

Identifier: 170077562

GI number: 170077562

Start: 971122

End: 972183

Strand: Direct

Name: dus1 [H]

Synonym: SYNPCC7002_A0940

Alternate gene names: 170077562

Gene position: 971122-972183 (Clockwise)

Preceding gene: 170077561

Following gene: 170077564

Centisome position: 32.28

GC content: 55.37

Gene sequence:

>1062_bases
GTGAATTCTGACCCAAGGCGATCGCCGCCAATCTCCCTGCCAGATAGCCCCTTAAAAATTGGTAACGTTGCCCTCCAGAG
CCGTGTCCTCCAAGCGCCCCTATCGGGGGTCACGGATCTAGTATTTCGTCGCCTGGTACGTCGCTACGCCCCCAGTTCAA
TGATGTACACCGAAATGGTGCGCGCCCAGGAAGTTCACCACCTCCAGACCTTACCGAGGCTCATGGAGATTGACCCGGGG
GAGCAGCCGATCAGCATTCAACTGTTTGACTGTCGCCCTGATTTTATGGGGGAAGCAGCAAAAAAAGCCGTGGCCGAGGG
CGCCCAAACCATCGACATCAACATGGGTTGTCCGGTGAATAAAATTACCCGCAAAGGAGGTGGTAGCTCCCTCCTGCGGC
AACCAGAGGTGGCGGTGGCGATCGTTAAAGCAGTGGTGGAAGCCGTCGATGTGCCTGTGACCGTTAAAACCCGCATTGGT
TGGGCTGATGCTGAAATCGTGATTGTTGACTTTGCCCGCCGGTTAGAAGATGCCGGAGCGCAAATGCTGACGGTGCACGG
TCGCACCCGGGCCCAGGGTTACACCGGGAAAGCCCAGTGGCAGTGGATTAAAAAAGTCAAAGAAGTCCTCTCGATTCCGG
TGATTGCCAACGGCGATATTTTTTCCGTGGATGCGGCGATCGCCTGCCTGGAAGCAACGGGAGCTGATGGGGTGATGTGT
TCCCGGGGTACCCTTGGTTATCCGTTTTTAGTCGGCGAAATTGACCATTTCCTCAAAACGGGACAGCGGCGGCCCCAACC
AACCGTGGTGGAGTTGCTCCAGTGCGCCAAGGAACATTTAGACGGGCTCTGGGCCTACAAAGGACAAAAGGGCATTTACC
AATCCCGCAAGCACCTGACTTGGTATTGTCGCGATTTTCCGGGGGCCGCAGATTTTCGCGATCGCCTTAATAAAATCAAC
ACCGTCGCCGAAGGTCAAGCCCTCCTGGATGAAGCCATTGAGCAAGTACAAAACCAAGCCAGCATTCCCGCCATCCAAAC
ACCCCAATTGGCGGTGATTTAA

Upstream 100 bases:

>100_bases
CCAGAACTAGAACAGCTACTCTTGTCCAAGACAATATTTCCCCATGATTCGGTAATCTTTGATGCCCCTGACCCCTAACA
CCACGGCCCCAGTAAATTCA

Downstream 100 bases:

>100_bases
CCCTTTTTGAGTTTACGAAACACCTCGTACATTTCCGGTAAACGCTTGTAGATGGCCGAAACTTTCCGGTAAACCGCATT
GGGCACCGCTGGACTACCAG

Product: dihydrouridine synthase (Dus)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNSDPRRSPPISLPDSPLKIGNVALQSRVLQAPLSGVTDLVFRRLVRRYAPSSMMYTEMVRAQEVHHLQTLPRLMEIDPG
EQPISIQLFDCRPDFMGEAAKKAVAEGAQTIDINMGCPVNKITRKGGGSSLLRQPEVAVAIVKAVVEAVDVPVTVKTRIG
WADAEIVIVDFARRLEDAGAQMLTVHGRTRAQGYTGKAQWQWIKKVKEVLSIPVIANGDIFSVDAAIACLEATGADGVMC
SRGTLGYPFLVGEIDHFLKTGQRRPQPTVVELLQCAKEHLDGLWAYKGQKGIYQSRKHLTWYCRDFPGAADFRDRLNKIN
TVAEGQALLDEAIEQVQNQASIPAIQTPQLAVI

Sequences:

>Translated_353_residues
MNSDPRRSPPISLPDSPLKIGNVALQSRVLQAPLSGVTDLVFRRLVRRYAPSSMMYTEMVRAQEVHHLQTLPRLMEIDPG
EQPISIQLFDCRPDFMGEAAKKAVAEGAQTIDINMGCPVNKITRKGGGSSLLRQPEVAVAIVKAVVEAVDVPVTVKTRIG
WADAEIVIVDFARRLEDAGAQMLTVHGRTRAQGYTGKAQWQWIKKVKEVLSIPVIANGDIFSVDAAIACLEATGADGVMC
SRGTLGYPFLVGEIDHFLKTGQRRPQPTVVELLQCAKEHLDGLWAYKGQKGIYQSRKHLTWYCRDFPGAADFRDRLNKIN
TVAEGQALLDEAIEQVQNQASIPAIQTPQLAVI
>Mature_353_residues
MNSDPRRSPPISLPDSPLKIGNVALQSRVLQAPLSGVTDLVFRRLVRRYAPSSMMYTEMVRAQEVHHLQTLPRLMEIDPG
EQPISIQLFDCRPDFMGEAAKKAVAEGAQTIDINMGCPVNKITRKGGGSSLLRQPEVAVAIVKAVVEAVDVPVTVKTRIG
WADAEIVIVDFARRLEDAGAQMLTVHGRTRAQGYTGKAQWQWIKKVKEVLSIPVIANGDIFSVDAAIACLEATGADGVMC
SRGTLGYPFLVGEIDHFLKTGQRRPQPTVVELLQCAKEHLDGLWAYKGQKGIYQSRKHLTWYCRDFPGAADFRDRLNKIN
TVAEGQALLDEAIEQVQNQASIPAIQTPQLAVI

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=300, Percent_Identity=29, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239788483, Length=296, Percent_Identity=30.0675675675676, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=35.5555555555556, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI40807366, Length=277, Percent_Identity=32.129963898917, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI8923374, Length=169, Percent_Identity=32.5443786982249, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=38.8198757763975, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788462, Length=258, Percent_Identity=37.2093023255814, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI145693211, Length=246, Percent_Identity=27.6422764227642, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=264, Percent_Identity=32.1969696969697, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=316, Percent_Identity=28.1645569620253, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=247, Percent_Identity=29.1497975708502, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=318, Percent_Identity=30.188679245283, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=30.5309734513274, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=316, Percent_Identity=25.3164556962025, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=36.0515021459227, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=36.0515021459227, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19921524, Length=303, Percent_Identity=30.3630363036304, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=31.25, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45549423, Length=148, Percent_Identity=32.4324324324324, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38815; Mature: 38815

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDPRRSPPISLPDSPLKIGNVALQSRVLQAPLSGVTDLVFRRLVRRYAPSSMMYTEMV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RAQEVHHLQTLPRLMEIDPGEQPISIQLFDCRPDFMGEAAKKAVAEGAQTIDINMGCPVN
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHH
KITRKGGGSSLLRQPEVAVAIVKAVVEAVDVPVTVKTRIGWADAEIVIVDFARRLEDAGA
HHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHHHHHHHHCCC
QMLTVHGRTRAQGYTGKAQWQWIKKVKEVLSIPVIANGDIFSVDAAIACLEATGADGVMC
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHCCCCCCEEE
SRGTLGYPFLVGEIDHFLKTGQRRPQPTVVELLQCAKEHLDGLWAYKGQKGIYQSRKHLT
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCEE
WYCRDFPGAADFRDRLNKINTVAEGQALLDEAIEQVQNQASIPAIQTPQLAVI
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
>Mature Secondary Structure
MNSDPRRSPPISLPDSPLKIGNVALQSRVLQAPLSGVTDLVFRRLVRRYAPSSMMYTEMV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RAQEVHHLQTLPRLMEIDPGEQPISIQLFDCRPDFMGEAAKKAVAEGAQTIDINMGCPVN
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHH
KITRKGGGSSLLRQPEVAVAIVKAVVEAVDVPVTVKTRIGWADAEIVIVDFARRLEDAGA
HHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHHHHHHHHCCC
QMLTVHGRTRAQGYTGKAQWQWIKKVKEVLSIPVIANGDIFSVDAAIACLEATGADGVMC
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHCCCCCCEEE
SRGTLGYPFLVGEIDHFLKTGQRRPQPTVVELLQCAKEHLDGLWAYKGQKGIYQSRKHLT
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCEE
WYCRDFPGAADFRDRLNKINTVAEGQALLDEAIEQVQNQASIPAIQTPQLAVI
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]