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The map label for this gene is purF [H]

Identifier: 170077175

GI number: 170077175

Start: 577569

End: 579038

Strand: Direct

Name: purF [H]

Synonym: SYNPCC7002_A0550

Alternate gene names: 170077175

Gene position: 577569-579038 (Clockwise)

Preceding gene: 170077174

Following gene: 170077176

Centisome position: 19.2

GC content: 52.99

Gene sequence:

>1470_bases
ATGACTGTCCCTCAAACTGTCTATGCCAACGATCTCCCCGACAAACCCAAGGAATACTGTGGTGTTTTCGGTATCTATGC
CCCAACGGAAGAAGTGGCAAAACTGGCATATTTCGGACTCTTTGCGCTCCAACATCGGGGGCAAGAATCCGCAGGGATCG
CGACCTTTGACGGTACGCAAATGCACGTGCACAAAGGTATGGGCCTTGTTTCCCAAGTCTTTAGCGAAGAAAAACTGCAG
GAACTGCCTGGGGTTTGGGCCGTGGGCCACAACCGCTACTCCACAACTGGCTCTAGCCATAAGTGCAATGCCCAGCCTGC
CCTTGAAGAAACTCGCCTGGGGACTTTGGCCCTCGCCCACAACGGCAACCTGGTGAACACCATCGAGCTGAAGGATAAGT
TGTTGTCCCTTGCCGATGGTCTAGATTTTCAGACCACCACAGACTCAGAAATGATCGCTAAGGCGATCGCCATCTATGTC
GATCAAGGGAAAGATTGGACTGAAGCGGCGATCGCTGCCTTTAACCTTTGTTCCGGTGCCTATAGCTTGGTCATCGGTAC
CCCGGACGGCATTATTGGCGCACGGGATCCCCAAGGAGTGCGTCCCCTCGTCATTGGCACCCTCACCGAGGAAGATGGCA
GCACCCGTTATGTTCTGGCTTCTGAAACCTGTGCCCTCGACATCATTGGCGCGGACTATCTGCGGGACGTCGAACCAGGC
GAAATGGTCTGGATTACCGAATCTGGCCTAACTGCCCAGCAGTGGTCTGAAAAACCAGACCGGAAGCTTTGTGTCTTCGA
GATGATCTATTTTGCCCGTCCCGATAGCATTGTCCACGACGAAACCCTCTTTAGTTACCGTCTCCGCCTGGGGGCGCAAT
TGGCCAAAGAATCCAACATTGAAGCCGATCTCGTCATGGGGGTTCCTGACTCTGGTATTCCCGCCGCCATCGGTTTTTCC
CGTGCCTCTGGGATTCCCTTTGGGGAAGGACTCATTAAAAACCGCTACGTAGGCCGTACCTTTATTCAACCGACCCAGCA
CATGCGGGAAGTGGGGATCCGCATGAAGCTCAACCCCCTAAAAGATGTCCTCCAAGGTAAACGAATTATCATCGTGGACG
ACTCCATTGTGCGCGGCACTACCAGCCGTAAAATTGTCCGGGCGCTCCGGCAAGCTGGGGCCGCTGAAGTCCATATGTGC
ATTTCCTCGCCCCCTGTGACCCATCCCTGTTTCTACGGCATCGACACCGACAGCCAAGATCAACTGATTGCTGCGACAAA
GTCCCTGGCTGAAATCGAAAAACAAATTGAAGTTGATTCCCTGACCTACCTCAGCAAAGAGGGCATGCTAGATGTCACCA
ACGAACGCACTTCTAGTTTCTGTACTGCTTGCTTCGATGGTCACTATCCCATTGAAATTCCGGACGCGATCAAGTCATCT
AAGCTAATGTTAGAAAAAGCCACTGTCTAG

Upstream 100 bases:

>100_bases
TTTGTCCCTCAAGGCATTCATTGACGTGGTATTGTAGCCATCGTAACCGCTGAATTTCCTTACTCTCTTTCACCCACTAA
CTAGGAGTGCAACCTGTAGG

Downstream 100 bases:

>100_bases
CAAAGCCGTTCCCATGGATTGAGAGCGGTAAAGGAAGTATTAATGCCGACCTATCCCGGAATTTCTAGCGAAGCCTTTCG
TCATCCCCTCGATCGCCAAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTVPQTVYANDLPDKPKEYCGVFGIYAPTEEVAKLAYFGLFALQHRGQESAGIATFDGTQMHVHKGMGLVSQVFSEEKLQ
ELPGVWAVGHNRYSTTGSSHKCNAQPALEETRLGTLALAHNGNLVNTIELKDKLLSLADGLDFQTTTDSEMIAKAIAIYV
DQGKDWTEAAIAAFNLCSGAYSLVIGTPDGIIGARDPQGVRPLVIGTLTEEDGSTRYVLASETCALDIIGADYLRDVEPG
EMVWITESGLTAQQWSEKPDRKLCVFEMIYFARPDSIVHDETLFSYRLRLGAQLAKESNIEADLVMGVPDSGIPAAIGFS
RASGIPFGEGLIKNRYVGRTFIQPTQHMREVGIRMKLNPLKDVLQGKRIIIVDDSIVRGTTSRKIVRALRQAGAAEVHMC
ISSPPVTHPCFYGIDTDSQDQLIAATKSLAEIEKQIEVDSLTYLSKEGMLDVTNERTSSFCTACFDGHYPIEIPDAIKSS
KLMLEKATV

Sequences:

>Translated_489_residues
MTVPQTVYANDLPDKPKEYCGVFGIYAPTEEVAKLAYFGLFALQHRGQESAGIATFDGTQMHVHKGMGLVSQVFSEEKLQ
ELPGVWAVGHNRYSTTGSSHKCNAQPALEETRLGTLALAHNGNLVNTIELKDKLLSLADGLDFQTTTDSEMIAKAIAIYV
DQGKDWTEAAIAAFNLCSGAYSLVIGTPDGIIGARDPQGVRPLVIGTLTEEDGSTRYVLASETCALDIIGADYLRDVEPG
EMVWITESGLTAQQWSEKPDRKLCVFEMIYFARPDSIVHDETLFSYRLRLGAQLAKESNIEADLVMGVPDSGIPAAIGFS
RASGIPFGEGLIKNRYVGRTFIQPTQHMREVGIRMKLNPLKDVLQGKRIIIVDDSIVRGTTSRKIVRALRQAGAAEVHMC
ISSPPVTHPCFYGIDTDSQDQLIAATKSLAEIEKQIEVDSLTYLSKEGMLDVTNERTSSFCTACFDGHYPIEIPDAIKSS
KLMLEKATV
>Mature_488_residues
TVPQTVYANDLPDKPKEYCGVFGIYAPTEEVAKLAYFGLFALQHRGQESAGIATFDGTQMHVHKGMGLVSQVFSEEKLQE
LPGVWAVGHNRYSTTGSSHKCNAQPALEETRLGTLALAHNGNLVNTIELKDKLLSLADGLDFQTTTDSEMIAKAIAIYVD
QGKDWTEAAIAAFNLCSGAYSLVIGTPDGIIGARDPQGVRPLVIGTLTEEDGSTRYVLASETCALDIIGADYLRDVEPGE
MVWITESGLTAQQWSEKPDRKLCVFEMIYFARPDSIVHDETLFSYRLRLGAQLAKESNIEADLVMGVPDSGIPAAIGFSR
ASGIPFGEGLIKNRYVGRTFIQPTQHMREVGIRMKLNPLKDVLQGKRIIIVDDSIVRGTTSRKIVRALRQAGAAEVHMCI
SSPPVTHPCFYGIDTDSQDQLIAATKSLAEIEKQIEVDSLTYLSKEGMLDVTNERTSSFCTACFDGHYPIEIPDAIKSSK
LMLEKATV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=512, Percent_Identity=41.015625, Blast_Score=358, Evalue=4e-99,
Organism=Escherichia coli, GI1788651, Length=470, Percent_Identity=39.7872340425532, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1790167, Length=231, Percent_Identity=30.7359307359307, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=41.9087136929461, Blast_Score=334, Evalue=8e-92,
Organism=Saccharomyces cerevisiae, GI6323958, Length=486, Percent_Identity=35.59670781893, Blast_Score=271, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=41.4285714285714, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28573187, Length=496, Percent_Identity=41.9354838709677, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=41.7558886509636, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53432; Mature: 53300

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPQTVYANDLPDKPKEYCGVFGIYAPTEEVAKLAYFGLFALQHRGQESAGIATFDGTQ
CCCCCCCCCCCCCCCCHHHHCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCE
MHVHKGMGLVSQVFSEEKLQELPGVWAVGHNRYSTTGSSHKCNAQPALEETRLGTLALAH
EEEHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEE
NGNLVNTIELKDKLLSLADGLDFQTTTDSEMIAKAIAIYVDQGKDWTEAAIAAFNLCSGA
CCCEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCC
YSLVIGTPDGIIGARDPQGVRPLVIGTLTEEDGSTRYVLASETCALDIIGADYLRDVEPG
EEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCEEEEEECHHHHCCCCCC
EMVWITESGLTAQQWSEKPDRKLCVFEMIYFARPDSIVHDETLFSYRLRLGAQLAKESNI
CEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EADLVMGVPDSGIPAAIGFSRASGIPFGEGLIKNRYVGRTFIQPTQHMREVGIRMKLNPL
CEEEEEECCCCCCCCEEECHHCCCCCCCCHHHHCCCCCCHHCCHHHHHHHCCCEEEECHH
KDVLQGKRIIIVDDSIVRGTTSRKIVRALRQAGAAEVHMCISSPPVTHPCFYGIDTDSQD
HHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCC
QLIAATKSLAEIEKQIEVDSLTYLSKEGMLDVTNERTSSFCTACFDGHYPIEIPDAIKSS
HHHHHHHHHHHHHHHHCHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCCCEECCHHHCCC
KLMLEKATV
CEEEEECCC
>Mature Secondary Structure 
TVPQTVYANDLPDKPKEYCGVFGIYAPTEEVAKLAYFGLFALQHRGQESAGIATFDGTQ
CCCCCCCCCCCCCCCHHHHCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCE
MHVHKGMGLVSQVFSEEKLQELPGVWAVGHNRYSTTGSSHKCNAQPALEETRLGTLALAH
EEEHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEE
NGNLVNTIELKDKLLSLADGLDFQTTTDSEMIAKAIAIYVDQGKDWTEAAIAAFNLCSGA
CCCEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCC
YSLVIGTPDGIIGARDPQGVRPLVIGTLTEEDGSTRYVLASETCALDIIGADYLRDVEPG
EEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCEEEEEECHHHHCCCCCC
EMVWITESGLTAQQWSEKPDRKLCVFEMIYFARPDSIVHDETLFSYRLRLGAQLAKESNI
CEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EADLVMGVPDSGIPAAIGFSRASGIPFGEGLIKNRYVGRTFIQPTQHMREVGIRMKLNPL
CEEEEEECCCCCCCCEEECHHCCCCCCCCHHHHCCCCCCHHCCHHHHHHHCCCEEEECHH
KDVLQGKRIIIVDDSIVRGTTSRKIVRALRQAGAAEVHMCISSPPVTHPCFYGIDTDSQD
HHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCC
QLIAATKSLAEIEKQIEVDSLTYLSKEGMLDVTNERTSSFCTACFDGHYPIEIPDAIKSS
HHHHHHHHHHHHHHHHCHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCCCEECCHHHCCC
KLMLEKATV
CEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]