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The map label for this gene is leuD [H]

Identifier: 170077082

GI number: 170077082

Start: 486052

End: 486645

Strand: Direct

Name: leuD [H]

Synonym: SYNPCC7002_A0456

Alternate gene names: 170077082

Gene position: 486052-486645 (Clockwise)

Preceding gene: 170077078

Following gene: 170077083

Centisome position: 16.16

GC content: 53.87

Gene sequence:

>594_bases
ATGAGCAGCGAAATCAAGCAAGTTTCTGGCACAGGGATCCCGCTCCGGGGCAACGACATCGACACAGACCGCATCATTCC
CGCACGGTTTCTGAAGTGTGTCACCTTTGATGGCCTTGGGGAACAAGCTTTTGCGGACGATCGCCAGCAACTAGACGGCG
AGCATCCCTTTGATTTACCCCAGTATCAGAATGCGAATATCCTCGTGGTCAACGGCAATTTCGGCTGCGGCTCTTCACGG
GAACACGCCCCCCAAGCCCTTTTGAAATGGGGTATTGAAGCCATTGTGGGTGAAAGTTTTGCGGAAATTTTCTTTGGAAA
CTGCCTCGCCAACGGCGTCCCCTGTGTAACGGCGGATCGGGCGACGGTCGAAGCATTACAAGGTCAGTTAGAAGCTAACC
CCCAGGCGGCCACCAGCGTCGATCTAGAAAATTTGACAGTTTCCTGCGGTGATTGGTCGGGTCAAATCCAGTTAGGAGAA
GGGGCTAGACAAGCATTACTCGCTGGCACTTGGGATAGTTGTGGTCAATTGGTGGCGAACCAGGCGGAGATTGCCACAAC
GGCGACAAAATTGCCTTACCTGGCCTGGGCCTAA

Upstream 100 bases:

>100_bases
TTTGGCGATCGCCCCTGGGGTTTCCTTTTCACTGTGGCAATTGCCCCAGGAAAGTATGCTAAATTAGCAATCTCCCACTG
GTCACCGATTCAAACGAGAT

Downstream 100 bases:

>100_bases
ACCATCATGGCCCAGTGGGAACTCTATAAAGAATTTCGCTTTGAGGCAGCCCACCAACTGCCCCACCACGATGGTAAATG
CGCCCGTCTCCACGGCCATA

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSSEIKQVSGTGIPLRGNDIDTDRIIPARFLKCVTFDGLGEQAFADDRQQLDGEHPFDLPQYQNANILVVNGNFGCGSSR
EHAPQALLKWGIEAIVGESFAEIFFGNCLANGVPCVTADRATVEALQGQLEANPQAATSVDLENLTVSCGDWSGQIQLGE
GARQALLAGTWDSCGQLVANQAEIATTATKLPYLAWA

Sequences:

>Translated_197_residues
MSSEIKQVSGTGIPLRGNDIDTDRIIPARFLKCVTFDGLGEQAFADDRQQLDGEHPFDLPQYQNANILVVNGNFGCGSSR
EHAPQALLKWGIEAIVGESFAEIFFGNCLANGVPCVTADRATVEALQGQLEANPQAATSVDLENLTVSCGDWSGQIQLGE
GARQALLAGTWDSCGQLVANQAEIATTATKLPYLAWA
>Mature_196_residues
SSEIKQVSGTGIPLRGNDIDTDRIIPARFLKCVTFDGLGEQAFADDRQQLDGEHPFDLPQYQNANILVVNGNFGCGSSRE
HAPQALLKWGIEAIVGESFAEIFFGNCLANGVPCVTADRATVEALQGQLEANPQAATSVDLENLTVSCGDWSGQIQLGEG
ARQALLAGTWDSCGQLVANQAEIATTATKLPYLAWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=36.8686868686869, Blast_Score=125, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321429, Length=124, Percent_Identity=41.1290322580645, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 20983; Mature: 20851

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEIKQVSGTGIPLRGNDIDTDRIIPARFLKCVTFDGLGEQAFADDRQQLDGEHPFDLP
CCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
QYQNANILVVNGNFGCGSSREHAPQALLKWGIEAIVGESFAEIFFGNCLANGVPCVTADR
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCH
ATVEALQGQLEANPQAATSVDLENLTVSCGDWSGQIQLGEGARQALLAGTWDSCGQLVAN
HHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCEEEECCCCHHHHHHCCHHHHHHHHHC
QAEIATTATKLPYLAWA
HHHHHHHHHHCCEEECC
>Mature Secondary Structure 
SSEIKQVSGTGIPLRGNDIDTDRIIPARFLKCVTFDGLGEQAFADDRQQLDGEHPFDLP
CCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
QYQNANILVVNGNFGCGSSREHAPQALLKWGIEAIVGESFAEIFFGNCLANGVPCVTADR
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCH
ATVEALQGQLEANPQAATSVDLENLTVSCGDWSGQIQLGEGARQALLAGTWDSCGQLVAN
HHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCEEEECCCCHHHHHHCCHHHHHHHHHC
QAEIATTATKLPYLAWA
HHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]