Click here to switch to the map view.

The map label for this gene is ptsA [H]

Identifier: 170025872

GI number: 170025872

Start: 4036932

End: 4039526

Strand: Reverse

Name: ptsA [H]

Synonym: YPK_3657

Alternate gene names: 170025872

Gene position: 4039526-4036932 (Counterclockwise)

Preceding gene: 170025876

Following gene: 170025864

Centisome position: 86.14

GC content: 52.49

Gene sequence:

>2595_bases
ATGACCAAGCAAGTGACATTTATCTGCGGGCTTCCTAATGGCGTTCATGCCCGACCCGCGAGCCATATAGAAAGGGTGTG
CAACCGCTTCCAGTCGCGTTTCCACTGGTATAATCCGCGTTCTGGCATCGTGGGGGATGGCAAGAGCGTACTTTCGCTGA
TCGCGACCAATATCCTGTTAGGGGATGAATGTCAGGTCACCATTGAAGGCGAAGATGAGCAGGCTGCTTTTGAACGCCTG
AGTATTTTTATTGAGCACGAGCTTCCACACGCTGACCCCATCTTGCCCAAGAGGGAGGAGAGTGCAGAATGGGAGCCGCT
CCCCGCCTCGTTAGCCCACTTGCACCCCACCCTGTTACGCGCCCGTTCAGTCAGTCCAGGTACCGCCTGCGGGAAGTTGC
TGTCTCTGATAAGAGCCGATCTCAATGCATTGGGTGATTTACCCGTGGCGCAAGGTATCGAGAGGGAGCAGCAGATGCTT
GCCGATGGGGTGGCGCAGCTAGGTAAAGCGTGGGAGAGCTTATTAGTCGCCAATAGCAGTACGGCAGCCAATAGCAGTAC
GACAGAAAATAGCAGTACGACAGAAAATAACAGTACGACAGAAAATAACAGTACGACAGAAAATAACAGCACGACGCGAG
CGATACGGGAAGTACACCGCTCGCTGTTGCGTGATGGCACATTTCGTCAACGGCTCTTGAGTCACATAATAGCAGGTGAA
AGCTGTGCGACAGCCATCGTCGCTACTGCGGCCTATTTCAGCCAGCAGTTGGCGCTTGCTGCTAACACTTATCTACGTGA
ACGTGAGTTGGATATTCGAGACGTCAGTTTTCAATTGTTACAGCAGATTTATGGTGAGCAACGTTTTCCCTCCCAGCAAG
CATTGAGCGAAGACAGTCTCTGCATTGCCGATGAGTTAACCCCGAGCCAATTCCTGGCGCTGGATAAACGTTATCTAAAA
GGGTTGCTATTGGGCCGTGGTGGCAGTACTTCACATACGGTGATTCTGGCGCGTTCATTCAATATTCCGACGCTCGTGGG
TGTCGATGCTACGGCACTCCAGCCGTATCTCAATCAATCGCTGCAGATTGATGGTGAGCTTGGGCTGGTGGTCTGTCTTT
TGGATGAGCCGGTGCGCCGTTACTATCGGCAGGAGCAGTGGCTACACGACCAACTGCGTGAGCAGCAGAGCCGATACCAG
AATATGCCTGGGCGTACTCTTGATGGTGTCAGAATGGTGGTGGCGGCTAACATCACCCATGCGGTAGAGGTGGAGGGTGC
TTTCAATCAAGGTGCCGAATCCATCGGCTTGTTTCGTACTGAAATGTTGTATATGGATCGCGCAGCCGCGCCTTCAGAAG
AGGAGCTTTATACCCTTTACGCTCAGGCCCTTGGCGCAGCCAAGGGCAAACCGATGATTATTCGCACCATCGATATTGGC
GGTGATAAACCGGTGAGTTATCTGAATATTCCTGCGGAGAGCAACCCGTTTCTGGGCTACCGCGCGGTACGTATTTATCA
CGAGTTTCTGTCGCTATTTCATACTCAATTGCGGGCGATCCTGCGGGCGTCAATGCATGGCCCGCTCAAAATCATGATCC
CGATGATCTCCTCAATGGAGGAGATCCTGTGGGTGAAAGATCAACTGGCAGAGGTGAAACAATCACTGCGTATCAATCAT
CTTCAGTTCGACGAAACTGTCCCTTTGGGTATGATGCTGGAAGTGCCTTCAGTGATGTTCATCATCGATCAATGCTGTGA
GGAAATGGATTTTTTGAGTATTGGCAGTAACGACCTAACACAGTACCTGCTGGCTGTTGATCGCGATAATGCCAAGGTGA
GTGAGCACTATCACTGCTTGTCCCCGGCACTCCTACGCGCTCTCGATTACGCGGTGTGCGAAGTACATCGCCACGGTAAG
TGGATTGGCTTATGTGGTGAACTGGCGGCAAAAGACTCGGTATTACCTCTGTTGGTAGCGATGGGGCTAGATGAGATCAG
CATGAGTGCCTCTTTTATTGGCGCGACTAAAGCTCGCCTTGCCAAACTTGATCGCGGCGAGTGCCGCCTGCTGCTCAACC
GGGCGATGGCTTGCCGCACATCACGGGAAGTAGAACATCTGCTGGTGCAGTACGACGGGGAACAAGGTGATGAACCACTG
ATTATTCCTGAATGTATTACTCTCGGTGTGGATTGGCGTAGCAAAGAAGAGGTGATCAAAGGGATGGTGGATAACCTGCT
GCTGGCTGGACGTTGCCGGTATCCGCGCAAACTGGCTGCGGATGTGTGGGCGCGAGAAGCCCTTTTTTCCACCGGATTGG
GCTTTGGGTTTGCCATTCCGCATGCTCAATCTGAGCATATTGAGCAGTCGACGATTAGCGTGGCCAAGTTGGCACAACCG
GTGAATTGGGGAGAGGAAGACGCACTGTTTGTGATGATGTTAACCCTGAAAAAACAGGCTTCGGGTGACCAGCATATGCG
TATTTTTTCCAAACTGGCGCGTCGGATCATGCACGAGGGTTTCCGCGAGGCGCTGGTTAGCGCTGGGACGGCCCAGCAAA
TAGAAACGCTGCTGAAACACGAGCTCGAACTGTGA

Upstream 100 bases:

>100_bases
TGTGATTACATTTGTCCAGTATTTGACATTTTTATCTCATAGCCGCGAGGGGATAGCCGCTTTTATGCTGGTTACCAGCC
AGCGAGGGGAGGAAAGCCCA

Downstream 100 bases:

>100_bases
TTCTTTCATTTCAACATGACGGGTGCCGCGTCTGAGGTGGCACCCAGTGACCCTTATCGGATTACTTCAATAAGAATGAC
TTTCCTTGCTTAAGCACTAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: MTP 2; Phosphoenolpyruvate-protein phosphotransferase; Enzyme I-Ani; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-like phosphotransferase enzyme IIA component; PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 864; Mature: 863

Protein sequence:

>864_residues
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTAANSSTTENSSTTENNSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGE
SCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLK
GLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQ
NMPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIG
GDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINH
LQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGK
WIGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPL
IIPECITLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQP
VNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL

Sequences:

>Translated_864_residues
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTAANSSTTENSSTTENNSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGE
SCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLK
GLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQ
NMPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIG
GDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINH
LQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGK
WIGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPL
IIPECITLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQP
VNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL
>Mature_863_residues
TKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERLS
IFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLA
DGVAQLGKAWESLLVANSSTAANSSTTENSSTTENNSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGES
CATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKG
LLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQN
MPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGG
DKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINHL
QFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKW
IGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLI
IPECITLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQPV
NWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=860, Percent_Identity=62.093023255814, Blast_Score=1064, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=864, Percent_Identity=41.3194444444444, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1788756, Length=453, Percent_Identity=38.6313465783664, Blast_Score=325, Evalue=8e-90,
Organism=Escherichia coli, GI1789193, Length=519, Percent_Identity=32.5626204238921, Blast_Score=251, Evalue=2e-67,
Organism=Escherichia coli, GI1787994, Length=412, Percent_Identity=27.1844660194175, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786951, Length=139, Percent_Identity=26.6187050359712, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 96105; Mature: 95974

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00369 PTS_HPR_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILL
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEE
GDECQVTIEGEDEQAAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAANSSTTENSSTTENNSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
SCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCE
CIADELTPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQS
EEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCC
LQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITH
EEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCE
AVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIG
EEEECCCHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC
GDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSME
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
EILWVKDQLAEVKQSLRINHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLT
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHH
MGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPL
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCE
IIPECITLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIP
EEHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECC
HAQSEHIEQSTISVAKLAQPVNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEG
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FREALVSAGTAQQIETLLKHELEL
HHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILL
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEE
GDECQVTIEGEDEQAAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAANSSTTENSSTTENNSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
SCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCE
CIADELTPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQS
EEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCC
LQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITH
EEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCE
AVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIG
EEEECCCHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC
GDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSME
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
EILWVKDQLAEVKQSLRINHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLT
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHH
MGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPL
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCE
IIPECITLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIP
EEHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECC
HAQSEHIEQSTISVAKLAQPVNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEG
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FREALVSAGTAQQIETLLKHELEL
HHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]