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The map label for this gene is yfiN [H]

Identifier: 170025831

GI number: 170025831

Start: 3988992

End: 3990302

Strand: Direct

Name: yfiN [H]

Synonym: YPK_3616

Alternate gene names: 170025831

Gene position: 3988992-3990302 (Clockwise)

Preceding gene: 170025830

Following gene: 170025832

Centisome position: 85.06

GC content: 48.51

Gene sequence:

>1311_bases
ATGAACACGATAAACACGATAAAAAACCAATCTATGAAGCCGAAAAAAAATCACAATAGCGCAGAAAAACTCCCGACACT
TGCTGGTGTCTTGCGGCGCATACATTTGTCTGTCGCCATTGTGTCTGTAGTAACAGCCTGTATTTTCCTCACCGTCGCCG
CGTTATTTGCCTTACGGGTTTATGTTGATCACAACCTGCATTTGATCGCCCGCGCCATCAGTTATACCACCGAGGCCGCC
GTCGTTTTTGGTGATAACGTGGAGGCTAACGAGTTATTGGCGCTGATTGCCTCGAATGAAGAAGTGGCAGAAGCGAAGAT
CATTGATAAACATAATCGTCAGATAGCTTACTGGGCGCTACCTGACGATGGGCCTATCCATCATATGGAGCAAATTGTTG
CCCGTTGGATACTGCCTGCCCCTGTCGTGTTACCGATGTTTCATGCTGGCGATGAGATTGGCAAAATTGTCATTGTTGGG
CATGGCGGCAGCTTATTGCGTTTCTTGCTACAAGGTCTGGCGGGGATGTTGGCGTGCCTGACGGTCAGTACATTGGTCGC
ACTTTTCCTATCACGGCGGATGTTGCTTGGTATTGTTGGCTCGCTTAAGAGGATCACTGAGGTGGCTCATAATGTCAGCC
GCCACCGCAGTTTTGGTCAACGGGTTCCTTCAGCAAAGGTGGCCGAATTGAACGAGCTCAGTAATGACTTTAACGGGTTG
CTGGAAGAGCTTGAGGTGTGGCAGAAACATCTGACGCAAGAAAATGACTCACTGGCTTACCGTGCCGCCCATGACAGCCT
GACCGGACTCGCGAATCGTACGTTTTTTGAAGGGCGCTTGAGCCGGACGCTTAATGATTGCCAACCCGGCGAACACACCG
CCGTGTTATTCCTGGATGGCGACCATTTTAAAGACGTCAATGATAATTATGGCCATGCCGCAGGCGATCTTGTGCTCACA
ACAATTGCCGTCCGTATCCGCGCCTTATTGCGTGAAACTGATTTAGTGGCTCGCTTAGGTGGCGATGAGTTTGCGGTGTT
ACTGACCCCTATCCACAACTTGGATGATGTTTTAACTATTGCCGATCATATTATCGACAGTATGGCCCTACCAATAATAC
TGGCTGAAGGCTGTGAAATTACCGTCTCACTCAGTATTGGTATTGCTGTTTATCCTGATCATGCACAAACGCCGGAAAAA
TTATTACAACGAGCGGATGAAGCGATGTATCAGGCGAAGAAAAACTTCCGGTCTGGCAAGCGTATTTTTGCCTATCAGCC
GAATACGACTAACACAGAAAGTTTAGGATAG

Upstream 100 bases:

>100_bases
AACAAGTCTCTTTTAAGGTCAATATGGATGCATTAGCACGCGGTGGTGTACGTATTCACCCTAGTGTATTGCAACTTGCC
CGTAAGAAGGCTAATGCACG

Downstream 100 bases:

>100_bases
GTGACATGCAGAAAATGCAAAACAAGCGTTTAATACAACGTATTTGGGTCAGTTTCCTGGCAATGGGTTTGATGCTATTA
GTGGGGTGTCAGGCTAAACC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNTINTIKNQSMKPKKNHNSAEKLPTLAGVLRRIHLSVAIVSVVTACIFLTVAALFALRVYVDHNLHLIARAISYTTEAA
VVFGDNVEANELLALIASNEEVAEAKIIDKHNRQIAYWALPDDGPIHHMEQIVARWILPAPVVLPMFHAGDEIGKIVIVG
HGGSLLRFLLQGLAGMLACLTVSTLVALFLSRRMLLGIVGSLKRITEVAHNVSRHRSFGQRVPSAKVAELNELSNDFNGL
LEELEVWQKHLTQENDSLAYRAAHDSLTGLANRTFFEGRLSRTLNDCQPGEHTAVLFLDGDHFKDVNDNYGHAAGDLVLT
TIAVRIRALLRETDLVARLGGDEFAVLLTPIHNLDDVLTIADHIIDSMALPIILAEGCEITVSLSIGIAVYPDHAQTPEK
LLQRADEAMYQAKKNFRSGKRIFAYQPNTTNTESLG

Sequences:

>Translated_436_residues
MNTINTIKNQSMKPKKNHNSAEKLPTLAGVLRRIHLSVAIVSVVTACIFLTVAALFALRVYVDHNLHLIARAISYTTEAA
VVFGDNVEANELLALIASNEEVAEAKIIDKHNRQIAYWALPDDGPIHHMEQIVARWILPAPVVLPMFHAGDEIGKIVIVG
HGGSLLRFLLQGLAGMLACLTVSTLVALFLSRRMLLGIVGSLKRITEVAHNVSRHRSFGQRVPSAKVAELNELSNDFNGL
LEELEVWQKHLTQENDSLAYRAAHDSLTGLANRTFFEGRLSRTLNDCQPGEHTAVLFLDGDHFKDVNDNYGHAAGDLVLT
TIAVRIRALLRETDLVARLGGDEFAVLLTPIHNLDDVLTIADHIIDSMALPIILAEGCEITVSLSIGIAVYPDHAQTPEK
LLQRADEAMYQAKKNFRSGKRIFAYQPNTTNTESLG
>Mature_436_residues
MNTINTIKNQSMKPKKNHNSAEKLPTLAGVLRRIHLSVAIVSVVTACIFLTVAALFALRVYVDHNLHLIARAISYTTEAA
VVFGDNVEANELLALIASNEEVAEAKIIDKHNRQIAYWALPDDGPIHHMEQIVARWILPAPVVLPMFHAGDEIGKIVIVG
HGGSLLRFLLQGLAGMLACLTVSTLVALFLSRRMLLGIVGSLKRITEVAHNVSRHRSFGQRVPSAKVAELNELSNDFNGL
LEELEVWQKHLTQENDSLAYRAAHDSLTGLANRTFFEGRLSRTLNDCQPGEHTAVLFLDGDHFKDVNDNYGHAAGDLVLT
TIAVRIRALLRETDLVARLGGDEFAVLLTPIHNLDDVLTIADHIIDSMALPIILAEGCEITVSLSIGIAVYPDHAQTPEK
LLQRADEAMYQAKKNFRSGKRIFAYQPNTTNTESLG

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=397, Percent_Identity=36.5239294710327, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=38.3333333333333, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=33.9080459770115, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=29.3785310734463, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=34.1040462427746, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=36.5384615384615, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=36.9942196531792, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=34.1317365269461, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=36.046511627907, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081977, Length=187, Percent_Identity=32.0855614973262, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787816, Length=178, Percent_Identity=30.3370786516854, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787056, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=85, Percent_Identity=38.8235294117647, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081921, Length=213, Percent_Identity=28.169014084507, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47950; Mature: 47950

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTINTIKNQSMKPKKNHNSAEKLPTLAGVLRRIHLSVAIVSVVTACIFLTVAALFALRV
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVDHNLHLIARAISYTTEAAVVFGDNVEANELLALIASNEEVAEAKIIDKHNRQIAYWAL
HHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHEEEECCCHHHHHHHHHCCCCEEEEEEC
PDDGPIHHMEQIVARWILPAPVVLPMFHAGDEIGKIVIVGHGGSLLRFLLQGLAGMLACL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
TVSTLVALFLSRRMLLGIVGSLKRITEVAHNVSRHRSFGQRVPSAKVAELNELSNDFNGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LEELEVWQKHLTQENDSLAYRAAHDSLTGLANRTFFEGRLSRTLNDCQPGEHTAVLFLDG
HHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
DHFKDVNDNYGHAAGDLVLTTIAVRIRALLRETDLVARLGGDEFAVLLTPIHNLDDVLTI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
ADHIIDSMALPIILAEGCEITVSLSIGIAVYPDHAQTPEKLLQRADEAMYQAKKNFRSGK
HHHHHHHHHHHHEEECCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RIFAYQPNTTNTESLG
EEEEECCCCCCCCCCC
>Mature Secondary Structure
MNTINTIKNQSMKPKKNHNSAEKLPTLAGVLRRIHLSVAIVSVVTACIFLTVAALFALRV
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVDHNLHLIARAISYTTEAAVVFGDNVEANELLALIASNEEVAEAKIIDKHNRQIAYWAL
HHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHEEEECCCHHHHHHHHHCCCCEEEEEEC
PDDGPIHHMEQIVARWILPAPVVLPMFHAGDEIGKIVIVGHGGSLLRFLLQGLAGMLACL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
TVSTLVALFLSRRMLLGIVGSLKRITEVAHNVSRHRSFGQRVPSAKVAELNELSNDFNGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LEELEVWQKHLTQENDSLAYRAAHDSLTGLANRTFFEGRLSRTLNDCQPGEHTAVLFLDG
HHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
DHFKDVNDNYGHAAGDLVLTTIAVRIRALLRETDLVARLGGDEFAVLLTPIHNLDDVLTI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
ADHIIDSMALPIILAEGCEITVSLSIGIAVYPDHAQTPEKLLQRADEAMYQAKKNFRSGK
HHHHHHHHHHHHEEECCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RIFAYQPNTTNTESLG
EEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]