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The map label for this gene is lpdA [H]

Identifier: 170025704

GI number: 170025704

Start: 3832318

End: 3833742

Strand: Reverse

Name: lpdA [H]

Synonym: YPK_3489

Alternate gene names: 170025704

Gene position: 3833742-3832318 (Counterclockwise)

Preceding gene: 170025705

Following gene: 170025703

Centisome position: 81.75

GC content: 48.7

Gene sequence:

>1425_bases
ATGAGTACTGAAATTAAAACTCAGGTCGTGGTACTTGGGGCAGGTCCAGCAGGGTACTCTGCTGCTTTTCGTTGTGCGGA
TTTAGGGTTAGAAACCATTCTGGTTGAACGTTACTCCACTCTGGGTGGGGTTTGCCTGAATGTGGGTTGTATCCCTTCCA
AGGCACTGTTACACGTTGCCAAAGTGATCGAAGAAGCCAAAGCGCTGGCTGAACATGGTATCGTTTTTGGCGAGCCTAAA
ACTGATATTGATAAAGTCCGTGTCTGGAAAGATAAAGTTATCAATCAGTTGACCGGTGGTTTGGCAGGTATGGCTAAAGG
CCGTAAAGTCAAAGTAGTGACTGGTTTTGGTAAATTTACCGGTGCGAACACCTTAGTGGTTGATGGTGAGAATGGTCCAA
CAACCATTAACTTCGATAACGCTATTATCGCGGCGGGTTCTCGCCCAATTCAACTGCCATTCATTCCTCATGAAGACTCA
CGTATTTGGGATTCAACTGACGCACTGGCATTGAGAACGGTTCCTGAGCGCTTGTTGGTGATGGGCGGTGGTATCATTGG
TCTGGAAATGGGGACCGTTTACCACGCACTGGGTTCTAAGATTGACGTGGTCGAAATGCTTGATCAGGTGATCCCCGCAG
CAGATAAAGACGTGGTGAAAGTCTTTACCAAGCGGATCAGCAAGCAGTTCAATCTGATGCTGGAAACCAAAGTGACAGCG
GTAGAAGCCAAAGAAGACGGTATCTATGTCACGATGGAAGGCAAAAAAGCGCCAGCAGAGCCACAACGCTATGATGCGGT
GTTGGTTGCGATTGGCCGCGTGCCTAACGGTAAGTTGCTGGATGCGGGTCAGGCGGGTGTTGAAGTTGATGATCGTGGCT
TTATCCACGTTGATAAGCAACTGCGCACCAATGTGCCACACATTTTTGCTATCGGTGACATCGTGGGTCAGCCAATGCTG
GCGCATAAAGGTGTCCACGAAGGCCATGTTGCCGCTGAAGTTATCGCAGGCATGAAGCACTATTTCGATCCGAAAGTTAT
TCCATCGATTGCGTACACTGAACCAGAAGTCGCATGGGTTGGTTTGACCGAAAAAGAAGCAAAAGAGAAAGGCATCAGCT
ACGAAACCTCCACCTTCCCGTGGGCGGCATCGGGCCGTGCTATCGCTTCTGATTGCGCAGATGGTATGACTAAACTGATT
TTCGATAAAGAAACTCACCGTATCATTGGTGGTGCGATTGTCGGTACTAACGGTGGCGAACTGTTAGGTGAAATCGGTCT
GGCCATTGAGATGGGTTGTGATGCAGAAGATATCGCATTGACCATTCATGCTCACCCAACATTGCATGAATCAGTGGGCC
TGGCGGCGGAAATCTACGAAGGTAGCATTACTGACCTGCCTAACCCGAAAGCGAAAAAGAAATAA

Upstream 100 bases:

>100_bases
GTTCCCGGTATAAGAGCGTCCCGGTGGATGAGGGCGTTATGAAATTGATTCGCCTAAAAAATGATGTCAGACCCGCCGGA
CAAACAATTAAGAGGTCATG

Downstream 100 bases:

>100_bases
TTTTTTGTTGATGCTGCATTTACAGATGTGAATGTACAGAGGTAAATGTACAGCAATAAATAAAAGTGAAACGGTCCCTA
CATAGGGGCCGTTTTTTTGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETILVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKVRVWKDKVINQLTGGLAGMAKGRKVKVVTGFGKFTGANTLVVDGENGPTTINFDNAIIAAGSRPIQLPFIPHEDS
RIWDSTDALALRTVPERLLVMGGGIIGLEMGTVYHALGSKIDVVEMLDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLDAGQAGVEVDDRGFIHVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLI
FDKETHRIIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK

Sequences:

>Translated_474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETILVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKVRVWKDKVINQLTGGLAGMAKGRKVKVVTGFGKFTGANTLVVDGENGPTTINFDNAIIAAGSRPIQLPFIPHEDS
RIWDSTDALALRTVPERLLVMGGGIIGLEMGTVYHALGSKIDVVEMLDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLDAGQAGVEVDDRGFIHVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLI
FDKETHRIIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
>Mature_473_residues
STEIKTQVVVLGAGPAGYSAAFRCADLGLETILVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKT
DIDKVRVWKDKVINQLTGGLAGMAKGRKVKVVTGFGKFTGANTLVVDGENGPTTINFDNAIIAAGSRPIQLPFIPHEDSR
IWDSTDALALRTVPERLLVMGGGIIGLEMGTVYHALGSKIDVVEMLDQVIPAADKDVVKVFTKRISKQFNLMLETKVTAV
EAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLDAGQAGVEVDDRGFIHVDKQLRTNVPHIFAIGDIVGQPMLA
HKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLIF
DKETHRIIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=42.9515418502203, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=27.7899343544858, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=27.212389380531, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=27.212389380531, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=27.212389380531, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=27.212389380531, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=27.212389380531, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=27.972027972028, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI291045266, Length=454, Percent_Identity=27.0925110132159, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI291045268, Length=446, Percent_Identity=25.3363228699552, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=93.8818565400844, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=460, Percent_Identity=28.9130434782609, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=27.6923076923077, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=28.604118993135, Blast_Score=149, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=448, Percent_Identity=40.625, Blast_Score=324, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=28.1512605042017, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.8348623853211, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=26.8348623853211, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=451, Percent_Identity=25.7206208425721, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.6666666666667, Blast_Score=310, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=27.0788912579957, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=27.6371308016878, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.7002188183807, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24640549, Length=462, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.0960334029228, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50535; Mature: 50404

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETILVERYSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKVRVWKDKVINQLTGGLAGMAKGRKVKVVTGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEECCCCC
GANTLVVDGENGPTTINFDNAIIAAGSRPIQLPFIPHEDSRIWDSTDALALRTVPERLLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHH
MGGGIIGLEMGTVYHALGSKIDVVEMLDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLDAGQAGVEVDDRGFIHVDKQ
EEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCEECCCCEEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLIFDKETHRIIGGAIVGTNGGE
ECCHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STEIKTQVVVLGAGPAGYSAAFRCADLGLETILVERYSTLGGVCLNVGCIPSKALLHVA
CCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKVRVWKDKVINQLTGGLAGMAKGRKVKVVTGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEECCCCC
GANTLVVDGENGPTTINFDNAIIAAGSRPIQLPFIPHEDSRIWDSTDALALRTVPERLLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHH
MGGGIIGLEMGTVYHALGSKIDVVEMLDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLDAGQAGVEVDDRGFIHVDKQ
EEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCEECCCCEEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLIFDKETHRIIGGAIVGTNGGE
ECCHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]