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The map label for this gene is aceF [H]

Identifier: 170025705

GI number: 170025705

Start: 3834048

End: 3835634

Strand: Reverse

Name: aceF [H]

Synonym: YPK_3490

Alternate gene names: 170025705

Gene position: 3835634-3834048 (Counterclockwise)

Preceding gene: 170025706

Following gene: 170025704

Centisome position: 81.79

GC content: 52.49

Gene sequence:

>1587_bases
ATGTCTATAGAAATTAATGTACCAGACATCGGTGCAGATGAAGTGGAAGTCACCGAAATTATGGTGAAAGTGGGCGATAC
CGTTGAAGCGGAACAGTCGCTAATCACCGTTGAAGGCGATAAAGCTTCCATGGAAGTTCCTTCACCTCAGGCGGGCGTGG
TTAAAGAGATCAAAATTGCGGTTGGCGATAAAGTGGCTACCGGTTCTCTGATCATGGTCTTCGACGCTACGGGTGCCGCT
GCGGCACCGGTTAAAGCAGAAGAAAAACCGGCGGCGCCTGCTCAGGCAGCGGCTCCGGCAGCCTCTGCGGCGAAAAATGT
TGAAGTGCCAGATATCGGTGATGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGATAAAGTTGACGCCGAAC
AATCACTGATTACGGTTGAAGGCGACAAAGCGTCGATGGAAGTGCCCGCACCGTTTGCTGGTATCGTGAAAGAAATCAAA
ATCAGTACCGGCGACAAAGTGAAAACCGGCTCTCTGATTATGGTCTTCGAAGTTGAAGGTGCAGCGCCAGCGCCAGCGCC
AGCAGCAGAAGCGGCTCCGGCTCAACAAGCCGCACCTGTCGCGCCAGCGCCAGCCGCCGCACCTGCCGCCAAAGCAGAAA
GCAAAGGCGAGTTTGCCGAGAATGACGCTTACGTGCATGCCACGCCGGTTATCCGTCGTCTGGCGCGTGAGTTCGGTGTG
AACCTGGCGAAGGTGAAAGGGACAGGCCGTAAGGGCCGTATCCTGCGCGAAGACATTCAAGCTTACGTGAAAGATGCCGT
GAAACGTGCCGAAGCTGCACCAGCAGCGGCTGGCGGCGGCCTGCCGGGCATGTTGCCTTGGCCAAAAGTTGATTTCAGTA
AATTTGGTGAAATCGAAGAAGTCGAATTGGGCCGTATCCAGAAAATTTCTGGTGCGAACCTGAGCCGTAACTGGGTCATG
ATCCCACATGTGACGCAATTCGATGAAGCGGATATCACTGAAGTTGAAGCCTTCCGTAAGCAACAGAACATCGAAGCTGA
GAAGAAAAAACAAGACCTGAAAATCACCCCGCTGGTGTTCCTGATGAAGGCCGCCGCTAAAGCACTGGAAGAATTCCCAC
GCTTTAACAGCTCCATTTCCGAAGATGGTCAGAAACTGACGCTGAAGAAATACATCAATATCGGTGTGGCGGTTGATACG
CCTAACGGCTTGGTAGTTCCAGTATTCCGTGACGTCAACAAAAAGGGTATTGTCGAGTTGTCTCGTGAGCTATCTGTCAT
CTCCAAGAAAGCACGTGATGGCAAGCTGACAGCATCTGACATGCAAGGCGGCTGTTTCACTATCTCCAGTCTGGGCGGTA
TCGGCGGTACGGCATTTACGCCAATCGTCAATGCGCCAGAAGTGGCTATCTTGGGTGTATCAAAATCATCCATGAAACCT
GTCTGGAATGGTAAAGAGTTTGCTCCACGCCTGATGTTACCGCTGTCTCTGTCCTTCGATCACCGTGTGATTGATGGTGC
CGCGGGTGCACGCTTCGCCGCGTATATCGCTACCATTATGGCGGATATTCGCCGTCTGGTGATGTAA

Upstream 100 bases:

>100_bases
CGCGGTGACATCGACACCAGTGTAGTTGCTGAAGCAATTACTAAGTTTGGTATCGACGCTGATAAAGTTAACCCGCGTCT
GGCATAAGAGGTAGAGAATA

Downstream 100 bases:

>100_bases
TCGCCAAGGCCGGCTTCGTGCCGGCCTTGTTGTGGTTACTGCTCTTGTTATTGGTGATCTTGTTATTACTGATCACCAAT
AGAGAAAAGACACTTATAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAA
AAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIK
ISTGDKVKTGSLIMVFEVEGAAPAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGV
NLAKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVM
IPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDT
PNGLVVPVFRDVNKKGIVELSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKP
VWNGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM

Sequences:

>Translated_528_residues
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAA
AAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIK
ISTGDKVKTGSLIMVFEVEGAAPAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGV
NLAKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVM
IPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDT
PNGLVVPVFRDVNKKGIVELSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKP
VWNGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM
>Mature_527_residues
SIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAAA
APVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKI
STGDKVKTGSLIMVFEVEGAAPAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVN
LAKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMI
PHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTP
NGLVVPVFRDVNKKGIVELSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPV
WNGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=31.7535545023697, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI31711992, Length=413, Percent_Identity=31.9612590799031, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI19923748, Length=206, Percent_Identity=38.3495145631068, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI203098816, Length=443, Percent_Identity=27.765237020316, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI203098753, Length=430, Percent_Identity=28.1395348837209, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI260898739, Length=142, Percent_Identity=37.3239436619718, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786305, Length=525, Percent_Identity=80, Blast_Score=791, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=406, Percent_Identity=31.7733990147783, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=412, Percent_Identity=30.0970873786408, Blast_Score=182, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=28.8372093023256, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=210, Percent_Identity=38.0952380952381, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=27.2435897435897, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=441, Percent_Identity=27.6643990929705, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=435, Percent_Identity=27.1264367816092, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=30.8612440191388, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=36.4485981308411, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=28.936170212766, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=28.936170212766, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 55621; Mature: 55489

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIA
CEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMVFDATGAAAAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVT
ECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCHHHHH
EVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKISTGDKVKTGSLIMVFEVEG
HHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHCCCCCCCEECCCEEEEEEECC
AAPAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGV
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC
NLAKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCE
VELGRIQKISGANLSRNWVMIPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVF
ECCCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEHHHHH
LMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVEL
HHHHHHHHHHHHHCCCCCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHH
SRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKP
HHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCC
VWNGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM
CCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIA
EEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMVFDATGAAAAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVT
ECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCHHHHH
EVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKISTGDKVKTGSLIMVFEVEG
HHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHCCCCCCCEECCCEEEEEEECC
AAPAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGV
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC
NLAKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCE
VELGRIQKISGANLSRNWVMIPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVF
ECCCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEHHHHH
LMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVEL
HHHHHHHHHHHHHCCCCCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHH
SRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKP
HHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCC
VWNGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM
CCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]