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The map label for this gene is glnS [H]

Identifier: 170025216

GI number: 170025216

Start: 3291365

End: 3293032

Strand: Reverse

Name: glnS [H]

Synonym: YPK_2994

Alternate gene names: 170025216

Gene position: 3293032-3291365 (Counterclockwise)

Preceding gene: 170025217

Following gene: 170025215

Centisome position: 70.22

GC content: 49.52

Gene sequence:

>1668_bases
ATGAGTGAGGCAGAAGCCCGCCCAAGTAATTTTATCCGTCAAATCATTGACGAAGATTTAGCCAGCGGTAAACACACGTC
GGTTCATACCCGTTTTCCGCCAGAGCCTAACGGCTATCTGCATATCGGCCATGCAAAATCTATTTGCCTGAATTTTGGTA
TTGCTGAAGATTATCAGGGCCAATGTAATTTACGCTTTGATGACACTAATCCAGTGAAAGAAGATGTCGAGTTCGTCGAG
TCAATTAAACGCGACGTAGAGTGGCTGGGCTTCACTTGGAGTGGTGATGTACGCTATTCCTCAGACTACTTTGATCAACT
GTATCAGTACGCTGTGGAGTTGATTAACAAGGGTCTTGCGTATGTGGATGAGCTGACCCCCGAGCAGATGCGCGAATATC
GTGGCACCTTGACTGCGCCGGGTAAAAATAGCCCATATCGTGACCGCAGTGTGGAAGAGAACCTGGTGCTGTTTGAAAAG
ATGCGTGCGGGGGGTTTTGCTGAAGGCACCGCCTGCTTACGGGCCAAAATTGACATGGCGTCACCGTTCATTGTGATGCG
TGACCCTGTGCTGTACCGCATCAAATTTGCAGAACATCATCAATCTGGCAATAAGTGGTGCATCTACCCGATGTATGACT
TCACCCATTGCATTTCCGATGCGCTGGAGGGGATCACTCATTCATTGTGTACGTTGGAATTCCAGGATAACCGTCGCTTG
TATGATTGGGTTCTGGATAATATCTCGATTGATTGTCATCCGCGTCAGTATGAGTTCTCCCGTCTGAACCTCGAATATAC
CATCATGTCTAAGCGTAAGCTGAACCAGTTGGTCACAGAGAAGGTCGTTGAGGGCTGGGATGATCCACGTATGCCGACCA
TTTCTGGCCTGCGCCGTCGTGGTTATACCGCAGCGTCTATCCGTGAGTTCTGCCGCCGTATTGGTGTCACCAAGCAGGAT
AACAATGTCGAAATGATGTCACTGGAATCGTGTATCCGTGATGATCTGAACGAACATGCGCCACGGGCGATGGCCGTGCT
TGACCCGATTAAGGTTGTTATTGAAAATCGCGCGGCGGGTGAAGAGTGGCTGACGATGCCAAACCACCCGAATAACCCAG
AAATGGGGAGCCGTCAGGTGCCATTCGATAGCGAAATCTACATTGATCGGGCTGATTTCCGCGAAGAAGCTAACAAGCAA
TATAAGCGCTTGGTCTTGGGCAAAGAAGTGCGCCTGCGTAATGCCTACGTGATCAAAGCTGAACGTGTTGAGAAAGACGC
AGAAGGCAATGTCACGACACTTTATTGCAGCTATGACGCCGAGACATTAAACAAAGATCCGGCTGATGGACGCAAAGTTA
AAGGGGTTATTCATTGGGTTTCTGTTGCCCATGCGCTGCCTGCGGAGATCCGTTTATACGATCGCTTGTTTAACGTACCA
AACCCAGCCGCGGCGGAAGACTTCCTGTCAACCATCAACCCTGAGTCCTTGGTTATCCGTCAGGGCTTTGTTGAGCCAAG
TCTGGCTGATGCGGTATCAGATAAAACCTATCAGTTTGAGCGTGAAGGTTATTTCTGTGCTGATAGCCGTTATTCACGTC
CCGGTGCTCTGGTGTTTAACCGTACTGTCGGCTTACGTGATACCTGGGCGGCGAAAGCCACTCAGTAA

Upstream 100 bases:

>100_bases
TTTATTTGTTTTGTTTGCCGTAACAAACGGTTGTTTCCGGGCCGGGCTTGTTGGATTATATGCGGTTATGTTTGTTGCGT
TTGCATTGAGGAATAGAACA

Downstream 100 bases:

>100_bases
ACTTTAGCGCTCAGATTCAATTGGCGTGGCACAGTCCACGCCTTTTTTGTCTCTGACAAACGGGTTCTGCTTGGTTTATT
AAACACCGGTTCCTGTCCTC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSEAEARPSNFIRQIIDEDLASGKHTSVHTRFPPEPNGYLHIGHAKSICLNFGIAEDYQGQCNLRFDDTNPVKEDVEFVE
SIKRDVEWLGFTWSGDVRYSSDYFDQLYQYAVELINKGLAYVDELTPEQMREYRGTLTAPGKNSPYRDRSVEENLVLFEK
MRAGGFAEGTACLRAKIDMASPFIVMRDPVLYRIKFAEHHQSGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
YDWVLDNISIDCHPRQYEFSRLNLEYTIMSKRKLNQLVTEKVVEGWDDPRMPTISGLRRRGYTAASIREFCRRIGVTKQD
NNVEMMSLESCIRDDLNEHAPRAMAVLDPIKVVIENRAAGEEWLTMPNHPNNPEMGSRQVPFDSEIYIDRADFREEANKQ
YKRLVLGKEVRLRNAYVIKAERVEKDAEGNVTTLYCSYDAETLNKDPADGRKVKGVIHWVSVAHALPAEIRLYDRLFNVP
NPAAAEDFLSTINPESLVIRQGFVEPSLADAVSDKTYQFEREGYFCADSRYSRPGALVFNRTVGLRDTWAAKATQ

Sequences:

>Translated_555_residues
MSEAEARPSNFIRQIIDEDLASGKHTSVHTRFPPEPNGYLHIGHAKSICLNFGIAEDYQGQCNLRFDDTNPVKEDVEFVE
SIKRDVEWLGFTWSGDVRYSSDYFDQLYQYAVELINKGLAYVDELTPEQMREYRGTLTAPGKNSPYRDRSVEENLVLFEK
MRAGGFAEGTACLRAKIDMASPFIVMRDPVLYRIKFAEHHQSGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
YDWVLDNISIDCHPRQYEFSRLNLEYTIMSKRKLNQLVTEKVVEGWDDPRMPTISGLRRRGYTAASIREFCRRIGVTKQD
NNVEMMSLESCIRDDLNEHAPRAMAVLDPIKVVIENRAAGEEWLTMPNHPNNPEMGSRQVPFDSEIYIDRADFREEANKQ
YKRLVLGKEVRLRNAYVIKAERVEKDAEGNVTTLYCSYDAETLNKDPADGRKVKGVIHWVSVAHALPAEIRLYDRLFNVP
NPAAAEDFLSTINPESLVIRQGFVEPSLADAVSDKTYQFEREGYFCADSRYSRPGALVFNRTVGLRDTWAAKATQ
>Mature_554_residues
SEAEARPSNFIRQIIDEDLASGKHTSVHTRFPPEPNGYLHIGHAKSICLNFGIAEDYQGQCNLRFDDTNPVKEDVEFVES
IKRDVEWLGFTWSGDVRYSSDYFDQLYQYAVELINKGLAYVDELTPEQMREYRGTLTAPGKNSPYRDRSVEENLVLFEKM
RAGGFAEGTACLRAKIDMASPFIVMRDPVLYRIKFAEHHQSGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRLY
DWVLDNISIDCHPRQYEFSRLNLEYTIMSKRKLNQLVTEKVVEGWDDPRMPTISGLRRRGYTAASIREFCRRIGVTKQDN
NVEMMSLESCIRDDLNEHAPRAMAVLDPIKVVIENRAAGEEWLTMPNHPNNPEMGSRQVPFDSEIYIDRADFREEANKQY
KRLVLGKEVRLRNAYVIKAERVEKDAEGNVTTLYCSYDAETLNKDPADGRKVKGVIHWVSVAHALPAEIRLYDRLFNVPN
PAAAEDFLSTINPESLVIRQGFVEPSLADAVSDKTYQFEREGYFCADSRYSRPGALVFNRTVGLRDTWAAKATQ

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=527, Percent_Identity=48.1973434535104, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI62241042, Length=518, Percent_Identity=35.5212355212355, Blast_Score=305, Evalue=7e-83,
Organism=Escherichia coli, GI1786895, Length=550, Percent_Identity=87.0909090909091, Blast_Score=1025, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=122, Percent_Identity=28.6885245901639, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543332, Length=534, Percent_Identity=45.1310861423221, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17511007, Length=524, Percent_Identity=33.206106870229, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324742, Length=560, Percent_Identity=41.4285714285714, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321192, Length=520, Percent_Identity=36.7307692307692, Blast_Score=310, Evalue=3e-85,
Organism=Drosophila melanogaster, GI17864488, Length=549, Percent_Identity=44.9908925318761, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24649466, Length=523, Percent_Identity=35.755258126195, Blast_Score=282, Evalue=4e-76,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 63780; Mature: 63648

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAEARPSNFIRQIIDEDLASGKHTSVHTRFPPEPNGYLHIGHAKSICLNFGIAEDYQG
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHEEEEECCCCCCCCC
QCNLRFDDTNPVKEDVEFVESIKRDVEWLGFTWSGDVRYSSDYFDQLYQYAVELINKGLA
CEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEECHHHHHHHHHHHHHHHHCCHH
YVDELTPEQMREYRGTLTAPGKNSPYRDRSVEENLVLFEKMRAGGFAEGTACLRAKIDMA
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHEEEEECCC
SPFIVMRDPVLYRIKFAEHHQSGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEEEECCCCEE
YDWVLDNISIDCHPRQYEFSRLNLEYTIMSKRKLNQLVTEKVVEGWDDPRMPTISGLRRR
EHHHHHCCEEECCCCCEEHEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GYTAASIREFCRRIGVTKQDNNVEMMSLESCIRDDLNEHAPRAMAVLDPIKVVIENRAAG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
EEWLTMPNHPNNPEMGSRQVPFDSEIYIDRADFREEANKQYKRLVLGKEVRLRNAYVIKA
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECCEEEEEH
ERVEKDAEGNVTTLYCSYDAETLNKDPADGRKVKGVIHWVSVAHALPAEIRLYDRLFNVP
HHHCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
NPAAAEDFLSTINPESLVIRQGFVEPSLADAVSDKTYQFEREGYFCADSRYSRPGALVFN
CCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCEEEECCCEEEECCCCCCCCEEEEE
RTVGLRDTWAAKATQ
ECCCCCCCCCCCCCC
>Mature Secondary Structure 
SEAEARPSNFIRQIIDEDLASGKHTSVHTRFPPEPNGYLHIGHAKSICLNFGIAEDYQG
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHEEEEECCCCCCCCC
QCNLRFDDTNPVKEDVEFVESIKRDVEWLGFTWSGDVRYSSDYFDQLYQYAVELINKGLA
CEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEECHHHHHHHHHHHHHHHHCCHH
YVDELTPEQMREYRGTLTAPGKNSPYRDRSVEENLVLFEKMRAGGFAEGTACLRAKIDMA
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHEEEEECCC
SPFIVMRDPVLYRIKFAEHHQSGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEEEECCCCEE
YDWVLDNISIDCHPRQYEFSRLNLEYTIMSKRKLNQLVTEKVVEGWDDPRMPTISGLRRR
EHHHHHCCEEECCCCCEEHEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GYTAASIREFCRRIGVTKQDNNVEMMSLESCIRDDLNEHAPRAMAVLDPIKVVIENRAAG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
EEWLTMPNHPNNPEMGSRQVPFDSEIYIDRADFREEANKQYKRLVLGKEVRLRNAYVIKA
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECCEEEEEH
ERVEKDAEGNVTTLYCSYDAETLNKDPADGRKVKGVIHWVSVAHALPAEIRLYDRLFNVP
HHHCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
NPAAAEDFLSTINPESLVIRQGFVEPSLADAVSDKTYQFEREGYFCADSRYSRPGALVFN
CCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCEEEECCCEEEECCCCCCCCEEEEE
RTVGLRDTWAAKATQ
ECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA