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The map label for this gene is nemA [H]

Identifier: 170025128

GI number: 170025128

Start: 3200506

End: 3201615

Strand: Reverse

Name: nemA [H]

Synonym: YPK_2906

Alternate gene names: 170025128

Gene position: 3201615-3200506 (Counterclockwise)

Preceding gene: 170025129

Following gene: 170025126

Centisome position: 68.27

GC content: 50.36

Gene sequence:

>1110_bases
ATGGACAAGCTATTTACACCATTTAAAGTCGGTGATACTGAATTGGTTAACCGCGTAGTGATGGCTCCTATGACACGCTC
ACGAGCAACGGTGGGTGATACTGCAGATGAACTGACGGCAAAGTACTACCAGCAGCGCGCATCGGCTGGGCTTATTATTA
CCGAAGGCTCGCAGATTTCCGTGCAGGGGCAGGGTTATCTCTTTACGCCCGGCATCTATAACGATGCACAGGTTAAAGGG
TGGGAAAGGGTTACGAACGCGGTCCACAATGCTGGTGGAAAAATCTACATTCAGCTCTGGCATGTTGGCCGTATTTCTCA
CACTGGTTTGCAGAATGACGGGCAGGCACCGGTCAGTTCAGTGGCAGTAAAAGCCAAGAATTCAACCTGCTATGCACGTG
ATGAGAATGGTCAGCCTGGCCTTGTTCCGGTGTCTGAACCGCGTGCACTGTCAGTGTCAGAGATTAAGGATATCGTTCAG
GATTACGTCCAAGCCGCAGAGAATGCTATTCGTGCGGGTTTTGATGGCGTTGAGATCCACGCGGCTAATGGTTACCTCCT
TGAGCAGTTCATCAATGGCGGGCTGAATACCCGACAAGATGAATACGGTGGTGCAGATCCTGAAAATCGTCTTCGCTTTG
TGTTGGAAGTCACTGATGCCATCTCACAGGCGATTGGTGCAGGAAAAACGGGGATTCGCCTGGCACCATTCGGACGCCTT
TTCGATATGCATCCCTTTGAGGGCGAGGAAGAAACCTGGCTACTGCTGGCAAAAGAACTCAGCACACGTTCGCTCTCCTA
TGTTCACTTGAGTGACCAGCAAACACTTGGCGAACAGGCTATTCCTGCCGGATTTATTGAGAAGTTTCGCGCTGCGTATG
ACGGCCCTTTGATTATTGCCGGAGGTTTTGACAAACAACGCGCCGAACAATATCTCCAGCAAGGAAAAGCCGATTTGATA
GGATTTGGGCGTCCTTATATTGCGAACCCGGATCTGGTCGAACGTATGGTCAATGACTGGCCATTAAATCAGGTTGATCG
GGCAACAATGTATGGCGGGGATGAAAAAGGGTATACAGACTATCCGGTCTGGTCCGATACCCAGGCATAA

Upstream 100 bases:

>100_bases
GCTTTAAAGTAGAATTTATGGAACCTCAGTTCGGAACAGATAGCTGTTCACTTATGGCTCCGCAGTAATCATCATGTTTC
TTTTATTAATGGAGAGATAA

Downstream 100 bases:

>100_bases
TCAGTCGAGGAGGCCGTCTTCTGCTGCCTTAACCAGCGTGTCCACAACGTAGCGTATTTTAGGCAGCAGTTGACGGTTTT
TCGGCCACAGCGCACTGATC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MDKLFTPFKVGDTELVNRVVMAPMTRSRATVGDTADELTAKYYQQRASAGLIITEGSQISVQGQGYLFTPGIYNDAQVKG
WERVTNAVHNAGGKIYIQLWHVGRISHTGLQNDGQAPVSSVAVKAKNSTCYARDENGQPGLVPVSEPRALSVSEIKDIVQ
DYVQAAENAIRAGFDGVEIHAANGYLLEQFINGGLNTRQDEYGGADPENRLRFVLEVTDAISQAIGAGKTGIRLAPFGRL
FDMHPFEGEEETWLLLAKELSTRSLSYVHLSDQQTLGEQAIPAGFIEKFRAAYDGPLIIAGGFDKQRAEQYLQQGKADLI
GFGRPYIANPDLVERMVNDWPLNQVDRATMYGGDEKGYTDYPVWSDTQA

Sequences:

>Translated_369_residues
MDKLFTPFKVGDTELVNRVVMAPMTRSRATVGDTADELTAKYYQQRASAGLIITEGSQISVQGQGYLFTPGIYNDAQVKG
WERVTNAVHNAGGKIYIQLWHVGRISHTGLQNDGQAPVSSVAVKAKNSTCYARDENGQPGLVPVSEPRALSVSEIKDIVQ
DYVQAAENAIRAGFDGVEIHAANGYLLEQFINGGLNTRQDEYGGADPENRLRFVLEVTDAISQAIGAGKTGIRLAPFGRL
FDMHPFEGEEETWLLLAKELSTRSLSYVHLSDQQTLGEQAIPAGFIEKFRAAYDGPLIIAGGFDKQRAEQYLQQGKADLI
GFGRPYIANPDLVERMVNDWPLNQVDRATMYGGDEKGYTDYPVWSDTQA
>Mature_369_residues
MDKLFTPFKVGDTELVNRVVMAPMTRSRATVGDTADELTAKYYQQRASAGLIITEGSQISVQGQGYLFTPGIYNDAQVKG
WERVTNAVHNAGGKIYIQLWHVGRISHTGLQNDGQAPVSSVAVKAKNSTCYARDENGQPGLVPVSEPRALSVSEIKDIVQ
DYVQAAENAIRAGFDGVEIHAANGYLLEQFINGGLNTRQDEYGGADPENRLRFVLEVTDAISQAIGAGKTGIRLAPFGRL
FDMHPFEGEEETWLLLAKELSTRSLSYVHLSDQQTLGEQAIPAGFIEKFRAAYDGPLIIAGGFDKQRAEQYLQQGKADLI
GFGRPYIANPDLVERMVNDWPLNQVDRATMYGGDEKGYTDYPVWSDTQA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=46.2809917355372, Blast_Score=313, Evalue=8e-87,
Organism=Escherichia coli, GI1789463, Length=350, Percent_Identity=28.2857142857143, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559804, Length=303, Percent_Identity=27.3927392739274, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=293, Percent_Identity=29.0102389078498, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=314, Percent_Identity=27.7070063694268, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=212, Percent_Identity=32.0754716981132, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=212, Percent_Identity=29.7169811320755, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=215, Percent_Identity=30.6976744186047, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=225, Percent_Identity=28.4444444444444, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6325086, Length=388, Percent_Identity=38.9175257731959, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321973, Length=391, Percent_Identity=37.5959079283887, Blast_Score=220, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40529; Mature: 40529

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLFTPFKVGDTELVNRVVMAPMTRSRATVGDTADELTAKYYQQRASAGLIITEGSQIS
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEE
VQGQGYLFTPGIYNDAQVKGWERVTNAVHNAGGKIYIQLWHVGRISHTGLQNDGQAPVSS
EECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCEECCCCCCCCCCCHHH
VAVKAKNSTCYARDENGQPGLVPVSEPRALSVSEIKDIVQDYVQAAENAIRAGFDGVEIH
EEEEECCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AANGYLLEQFINGGLNTRQDEYGGADPENRLRFVLEVTDAISQAIGAGKTGIRLAPFGRL
ECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHCCCCCCEEECCCCHH
FDMHPFEGEEETWLLLAKELSTRSLSYVHLSDQQTLGEQAIPAGFIEKFRAAYDGPLIIA
HCCCCCCCCCCEEEEEEEHHCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCCEEEE
GGFDKQRAEQYLQQGKADLIGFGRPYIANPDLVERMVNDWPLNQVDRATMYGGDEKGYTD
CCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
YPVWSDTQA
CCCCCCCCC
>Mature Secondary Structure
MDKLFTPFKVGDTELVNRVVMAPMTRSRATVGDTADELTAKYYQQRASAGLIITEGSQIS
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEE
VQGQGYLFTPGIYNDAQVKGWERVTNAVHNAGGKIYIQLWHVGRISHTGLQNDGQAPVSS
EECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCEECCCCCCCCCCCHHH
VAVKAKNSTCYARDENGQPGLVPVSEPRALSVSEIKDIVQDYVQAAENAIRAGFDGVEIH
EEEEECCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AANGYLLEQFINGGLNTRQDEYGGADPENRLRFVLEVTDAISQAIGAGKTGIRLAPFGRL
ECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHCCCCCCEEECCCCHH
FDMHPFEGEEETWLLLAKELSTRSLSYVHLSDQQTLGEQAIPAGFIEKFRAAYDGPLIIA
HCCCCCCCCCCEEEEEEEHHCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCCEEEE
GGFDKQRAEQYLQQGKADLIGFGRPYIANPDLVERMVNDWPLNQVDRATMYGGDEKGYTD
CCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
YPVWSDTQA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]