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The map label for this gene is lolB [H]

Identifier: 170024412

GI number: 170024412

Start: 2420402

End: 2421025

Strand: Direct

Name: lolB [H]

Synonym: YPK_2181

Alternate gene names: 170024412

Gene position: 2420402-2421025 (Clockwise)

Preceding gene: 170024405

Following gene: 170024413

Centisome position: 51.61

GC content: 48.08

Gene sequence:

>624_bases
ATGCCCATGCGTAAAAGACATTTTTATCGCCTGCTGCCTTTAGCCTCTCTGCTGCTGGCTGCTTGTACAATCCCAGTATC
AAAGGGACCAGCGACTAGCCCTACGTCGCCACAGTGGCGTCAGCATGAACAACAGTTGCAGCAACTTGGCCAATTTGAAA
CTCGTGGCGCATTTGCTTACCTGTCTGATAAACAGAAAGTTTATGCCAGATTCTTTTGGCAACAGACCTCTCCCGAGCGT
TATCGTTTATTACTGACTAACCCGTTAGGCAGCACTGAGTTGGAGTTGGTTGTCCAACCGGGTGTGACACAACTGACGGA
TAATCAAGGTAAGCACTACGTCAGTGATGATCCGCAAGAGATGATTCAAAAGTTGACGGGCATGTCCATCCCACTGGAAA
GCCTGCGCCAATGGATCCTTGGTTTACCGGGCGATACCCCTAATTTTACCCTTGACGATAAATATCGCCTGAAGAAACTG
ACTTACCAACAAAATGGGGTGACATGGGTTGTTGATTATCAAGAATACAACACGCAGGTCACGCCCCCCTTACCCAGCCG
CCTGGAGCTGAATCAAGATGGGCAACGGATTAAACTGAAAATGGATAGCTGGACGATCAAATAA

Upstream 100 bases:

>100_bases
TATTTGCAACGAATCAAAACGTGTTGTTTTGCCTTAAATGTTGCCTCATCAGAAACCTGCCGCAGGTGAAAACCAGACGG
CTGATAGGATTTGTACCCAT

Downstream 100 bases:

>100_bases
CATGACTACCGCGAATCAACCTATATGTCCGTCACCAGCAAAATGGCCGTCACCGGCAAAATTGAATTTGTTTCTTTATA
TCACCGGGCAACGCGCCGAC

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFAYLSDKQKVYARFFWQQTSPER
YRLLLTNPLGSTELELVVQPGVTQLTDNQGKHYVSDDPQEMIQKLTGMSIPLESLRQWILGLPGDTPNFTLDDKYRLKKL
TYQQNGVTWVVDYQEYNTQVTPPLPSRLELNQDGQRIKLKMDSWTIK

Sequences:

>Translated_207_residues
MPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFAYLSDKQKVYARFFWQQTSPER
YRLLLTNPLGSTELELVVQPGVTQLTDNQGKHYVSDDPQEMIQKLTGMSIPLESLRQWILGLPGDTPNFTLDDKYRLKKL
TYQQNGVTWVVDYQEYNTQVTPPLPSRLELNQDGQRIKLKMDSWTIK
>Mature_206_residues
PMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFAYLSDKQKVYARFFWQQTSPERY
RLLLTNPLGSTELELVVQPGVTQLTDNQGKHYVSDDPQEMIQKLTGMSIPLESLRQWILGLPGDTPNFTLDDKYRLKKLT
YQQNGVTWVVDYQEYNTQVTPPLPSRLELNQDGQRIKLKMDSWTIK

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=207, Percent_Identity=62.8019323671498, Blast_Score=281, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 23982; Mature: 23851

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFAY
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEE
LSDKQKVYARFFWQQTSPERYRLLLTNPLGSTELELVVQPGVTQLTDNQGKHYVSDDPQE
ECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCEECCCCCCCCCCCCHHH
MIQKLTGMSIPLESLRQWILGLPGDTPNFTLDDKYRLKKLTYQQNGVTWVVDYQEYNTQV
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCEECCHHHHHHHEEECCCCEEEEEECCCCCEE
TPPLPSRLELNQDGQRIKLKMDSWTIK
CCCCCCCEEECCCCCEEEEEECCEECC
>Mature Secondary Structure 
PMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFAY
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEE
LSDKQKVYARFFWQQTSPERYRLLLTNPLGSTELELVVQPGVTQLTDNQGKHYVSDDPQE
ECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCEECCCCCCCCCCCCHHH
MIQKLTGMSIPLESLRQWILGLPGDTPNFTLDDKYRLKKLTYQQNGVTWVVDYQEYNTQV
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCEECCHHHHHHHEEECCCCEEEEEECCCCCEE
TPPLPSRLELNQDGQRIKLKMDSWTIK
CCCCCCCEEECCCCCEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA