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The map label for this gene is sppA [H]

Identifier: 170024325

GI number: 170024325

Start: 2320125

End: 2321975

Strand: Direct

Name: sppA [H]

Synonym: YPK_2091

Alternate gene names: 170024325

Gene position: 2320125-2321975 (Clockwise)

Preceding gene: 170024320

Following gene: 170024326

Centisome position: 49.48

GC content: 48.14

Gene sequence:

>1851_bases
ATGCGCACTTTGTGGCGAATTATTGCTGGTTTTTTCAAGTGGACCTGGCGTCTACTGAATTTCACCAGAGAGCTTATCCT
TAATCTTTTTTTGGCACTGCTTATTTTGATCGGTGTCGGTATTTATTTTCAATTTCAAAGTAAGCCTGTGGAGCCAGTTA
AAGGTGCCCTGCTGGTAAATTTAAGCGGTGTGATCGTAGATCAACCTGCTATCAATAATAAACTACGCCAATGGGGACGT
GAGCTATTAGGGGCATCCAGTAACCGCCTACAGGAAAACTCCCTGTTTGATATTGTAGAAACCATTCGACTGGCAAAAGA
CGATGACAATATTAACGGCCTGGTTCTGTCTCTGAGTGACTTAACGGGGGCAGATCAGCCCTCGCTGCAATATATTGGTA
AGGCTCTACGGGAATTTCGCGACACCGGCAAGAAAATTTATGCCGTCGGCGACAGCTACAATCAAACCCAATACTATTTA
GCCAGTTTTGCTAATAAGATTTATCTCTCGCCACAAGGGGCTGTTGACCTACACGGTTTTGCCAGCAATAACCTTTATTA
CAAGTCACTGCTGGAAAATCTTAAAGTCACGACCAATATCTTCCGCGTAGGGACTTATAAATCAGCAGTTGAACCGATGA
TCCGTAATGATATGTCCGCCGCTGCCCGTGAAGCCGATAGCCGCTGGGTGGGTGGCTTGTGGCAAAACTACCTCACCACG
GTCTCCGCTAACCGCCGACTCACCCCAGAACAACTGTTCCCTGGGGCCGCAGGGGTGATCAGTGGCTTACAGGTAGCGGG
TGGCTCACAAGCTAAATACGCACTGGATAGCAAACTGGTAGACCAATTAGCAGCCCGACCAGAAGTAGAAAGTGCACTGG
TTGAAGCCTTTGGCTGGAATAAAAAGACTAATGACTTCAACTACATCAGTATTTATGACTATCAGCCAACACCCGCACCA
CAGCAAGGGGAACAGATAGCGGTTCTCTTCGCTAACGGAGCAATTATTGACGGCCCGCAAACCCCTGGGAACGTGGGGGG
AGATACGCTGGCAGCACAAATTCGCCAGGCCCGTTTAGATCCAAAGATTAAAGCGGTTATCTTGCGTGTAAACAGCCCTG
GCGGCAGTGTGAGCGCTTCTGAACTGATCCGCGCCGAATTGGCTGCATTACGTGCTGCCCATAAGCCATTGGTGGTATCA
ATGGGTGGAATGGCGGCTTCTGGTGGATATTGGATCTCAACACCAGCGAACTATATCGTTGCCAGCCCAAGCACTCTGAC
CGGCTCCATTGGTATTTTCGGTGTGATTAACACCTTCCAAAATTCACTGGCAAGTATTGGTGTCCATACAGACGGTGTCG
CGACATCACCATTAGCTGACGTGTCACTGACCAAAGCATTGCCGCCTGAGTTCTCTCAGATGATGCAAATCAATATCGAA
AATGGGTATAAAACCTTTATTGATTTGGTTGCAACTTCTCGCCACAAGACCCCTGAGCAAGTGGATCAAATTGCACAAGG
CCATGTGTGGATTGGCCTCGATGCTAAAAGTAACGGTTTAGTCGATCAACTCGGCGATTTTGATGATGCCGTGAAAAAGG
CGGCAGAACTGGCCAAACTGAAGACTTGGCAATTGAATTGGTTTGTTGATGAGCCAAGCTTAAGCGACCTTATTTTGGGT
CAAATGAGCGCGTCGGTGCACGCTATGTTGCCAGCGGCTATTCAGACGTGGTTACCCGCGCCTTTATCTGCTATGGCGCT
TGCGGTTAAAGATCAACCTGGTTTATTCAATACCCTGAATGATCCGCAGAATCGTTATGCTCTGTGCCTAACCTGTGGTG
ATGTCCGCTAA

Upstream 100 bases:

>100_bases
AGCGTTGACCTATCAGCTAGGCTTATCGATGATTTAAAGCTGACTTTTTTTCATCAGGTCATTAGGATAACCCTACTTTC
CCGGTTTTGTTGGAGATCAT

Downstream 100 bases:

>100_bases
TAATCGTCCTAAAATACAGATAAATATTCAGCCCGATACCCATCGGGCTGTTTTTCCTCGCTGAGCTCCTTATAATTCAA
TCATCCAATCCCAGCATTAC

Product: protease 4

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MRTLWRIIAGFFKWTWRLLNFTRELILNLFLALLILIGVGIYFQFQSKPVEPVKGALLVNLSGVIVDQPAINNKLRQWGR
ELLGASSNRLQENSLFDIVETIRLAKDDDNINGLVLSLSDLTGADQPSLQYIGKALREFRDTGKKIYAVGDSYNQTQYYL
ASFANKIYLSPQGAVDLHGFASNNLYYKSLLENLKVTTNIFRVGTYKSAVEPMIRNDMSAAAREADSRWVGGLWQNYLTT
VSANRRLTPEQLFPGAAGVISGLQVAGGSQAKYALDSKLVDQLAARPEVESALVEAFGWNKKTNDFNYISIYDYQPTPAP
QQGEQIAVLFANGAIIDGPQTPGNVGGDTLAAQIRQARLDPKIKAVILRVNSPGGSVSASELIRAELAALRAAHKPLVVS
MGGMAASGGYWISTPANYIVASPSTLTGSIGIFGVINTFQNSLASIGVHTDGVATSPLADVSLTKALPPEFSQMMQINIE
NGYKTFIDLVATSRHKTPEQVDQIAQGHVWIGLDAKSNGLVDQLGDFDDAVKKAAELAKLKTWQLNWFVDEPSLSDLILG
QMSASVHAMLPAAIQTWLPAPLSAMALAVKDQPGLFNTLNDPQNRYALCLTCGDVR

Sequences:

>Translated_616_residues
MRTLWRIIAGFFKWTWRLLNFTRELILNLFLALLILIGVGIYFQFQSKPVEPVKGALLVNLSGVIVDQPAINNKLRQWGR
ELLGASSNRLQENSLFDIVETIRLAKDDDNINGLVLSLSDLTGADQPSLQYIGKALREFRDTGKKIYAVGDSYNQTQYYL
ASFANKIYLSPQGAVDLHGFASNNLYYKSLLENLKVTTNIFRVGTYKSAVEPMIRNDMSAAAREADSRWVGGLWQNYLTT
VSANRRLTPEQLFPGAAGVISGLQVAGGSQAKYALDSKLVDQLAARPEVESALVEAFGWNKKTNDFNYISIYDYQPTPAP
QQGEQIAVLFANGAIIDGPQTPGNVGGDTLAAQIRQARLDPKIKAVILRVNSPGGSVSASELIRAELAALRAAHKPLVVS
MGGMAASGGYWISTPANYIVASPSTLTGSIGIFGVINTFQNSLASIGVHTDGVATSPLADVSLTKALPPEFSQMMQINIE
NGYKTFIDLVATSRHKTPEQVDQIAQGHVWIGLDAKSNGLVDQLGDFDDAVKKAAELAKLKTWQLNWFVDEPSLSDLILG
QMSASVHAMLPAAIQTWLPAPLSAMALAVKDQPGLFNTLNDPQNRYALCLTCGDVR
>Mature_616_residues
MRTLWRIIAGFFKWTWRLLNFTRELILNLFLALLILIGVGIYFQFQSKPVEPVKGALLVNLSGVIVDQPAINNKLRQWGR
ELLGASSNRLQENSLFDIVETIRLAKDDDNINGLVLSLSDLTGADQPSLQYIGKALREFRDTGKKIYAVGDSYNQTQYYL
ASFANKIYLSPQGAVDLHGFASNNLYYKSLLENLKVTTNIFRVGTYKSAVEPMIRNDMSAAAREADSRWVGGLWQNYLTT
VSANRRLTPEQLFPGAAGVISGLQVAGGSQAKYALDSKLVDQLAARPEVESALVEAFGWNKKTNDFNYISIYDYQPTPAP
QQGEQIAVLFANGAIIDGPQTPGNVGGDTLAAQIRQARLDPKIKAVILRVNSPGGSVSASELIRAELAALRAAHKPLVVS
MGGMAASGGYWISTPANYIVASPSTLTGSIGIFGVINTFQNSLASIGVHTDGVATSPLADVSLTKALPPEFSQMMQINIE
NGYKTFIDLVATSRHKTPEQVDQIAQGHVWIGLDAKSNGLVDQLGDFDDAVKKAAELAKLKTWQLNWFVDEPSLSDLILG
QMSASVHAMLPAAIQTWLPAPLSAMALAVKDQPGLFNTLNDPQNRYALCLTCGDVR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=618, Percent_Identity=67.7993527508091, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1787527, Length=170, Percent_Identity=31.7647058823529, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 67008; Mature: 67008

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLWRIIAGFFKWTWRLLNFTRELILNLFLALLILIGVGIYFQFQSKPVEPVKGALLVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEE
LSGVIVDQPAINNKLRQWGRELLGASSNRLQENSLFDIVETIRLAKDDDNINGLVLSLSD
ECCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
LTGADQPSLQYIGKALREFRDTGKKIYAVGDSYNQTQYYLASFANKIYLSPQGAVDLHGF
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCEEEECCCCCEEEEEC
ASNNLYYKSLLENLKVTTNIFRVGTYKSAVEPMIRNDMSAAAREADSRWVGGLWQNYLTT
CCCCEEHHHHHHCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VSANRRLTPEQLFPGAAGVISGLQVAGGSQAKYALDSKLVDQLAARPEVESALVEAFGWN
HCCCCCCCHHHHCCCHHHHHHCCEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
KKTNDFNYISIYDYQPTPAPQQGEQIAVLFANGAIIDGPQTPGNVGGDTLAAQIRQARLD
CCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCHHHHHHHHHHHHCC
PKIKAVILRVNSPGGSVSASELIRAELAALRAAHKPLVVSMGGMAASGGYWISTPANYIV
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEECCCCEEE
ASPSTLTGSIGIFGVINTFQNSLASIGVHTDGVATSPLADVSLTKALPPEFSQMMQINIE
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHEEEEEC
NGYKTFIDLVATSRHKTPEQVDQIAQGHVWIGLDAKSNGLVDQLGDFDDAVKKAAELAKL
CCHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHH
KTWQLNWFVDEPSLSDLILGQMSASVHAMLPAAIQTWLPAPLSAMALAVKDQPGLFNTLN
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCC
DPQNRYALCLTCGDVR
CCCCCEEEEEEECCCC
>Mature Secondary Structure
MRTLWRIIAGFFKWTWRLLNFTRELILNLFLALLILIGVGIYFQFQSKPVEPVKGALLVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEE
LSGVIVDQPAINNKLRQWGRELLGASSNRLQENSLFDIVETIRLAKDDDNINGLVLSLSD
ECCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
LTGADQPSLQYIGKALREFRDTGKKIYAVGDSYNQTQYYLASFANKIYLSPQGAVDLHGF
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCEEEECCCCCEEEEEC
ASNNLYYKSLLENLKVTTNIFRVGTYKSAVEPMIRNDMSAAAREADSRWVGGLWQNYLTT
CCCCEEHHHHHHCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VSANRRLTPEQLFPGAAGVISGLQVAGGSQAKYALDSKLVDQLAARPEVESALVEAFGWN
HCCCCCCCHHHHCCCHHHHHHCCEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
KKTNDFNYISIYDYQPTPAPQQGEQIAVLFANGAIIDGPQTPGNVGGDTLAAQIRQARLD
CCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCHHHHHHHHHHHHCC
PKIKAVILRVNSPGGSVSASELIRAELAALRAAHKPLVVSMGGMAASGGYWISTPANYIV
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEECCCCEEE
ASPSTLTGSIGIFGVINTFQNSLASIGVHTDGVATSPLADVSLTKALPPEFSQMMQINIE
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHEEEEEC
NGYKTFIDLVATSRHKTPEQVDQIAQGHVWIGLDAKSNGLVDQLGDFDDAVKKAAELAKL
CCHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHH
KTWQLNWFVDEPSLSDLILGQMSASVHAMLPAAIQTWLPAPLSAMALAVKDQPGLFNTLN
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCC
DPQNRYALCLTCGDVR
CCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]