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The map label for this gene is rbsB [H]

Identifier: 170023061

GI number: 170023061

Start: 927586

End: 928545

Strand: Direct

Name: rbsB [H]

Synonym: YPK_0812

Alternate gene names: 170023061

Gene position: 927586-928545 (Clockwise)

Preceding gene: 170023060

Following gene: 170023062

Centisome position: 19.78

GC content: 47.6

Gene sequence:

>960_bases
ATGAAAAAGAGACTAATTGGCCTGCTGTTGGCCGGTGTCACCATCTCAATGAGTGCCTCTGCCGCAGAGCCACTCAAGAT
CGGCTTCGCTAACCGTACCTTGAATGGCGCTTTCTTTAATGGGCTGACCGAATACATGAAGATTCATGCTAAGGAGAAAG
GCTATACATTAATTACCACCGATGCCCGCGGCGATTTGAATAAGCAAATTGCTGATGTGGAGGACATGCTGTCACAGGGG
ATTAATTATCTGATACTCAATCCGCAAGATCCCCAAGCGGGTTTGCGCATTACTGAAATTGCCAAGCGGGCTGGTGTACC
TACTGTGATCCTCGATAGCGATATCGCACTTGAAGCTCCGGTTATCACGCGAGTTCAGGCCAATAATGCCAAAAATAACA
ACCTGATCGGCGAATATGCGGTGGGCCAGTTTGGCGACAAACCGATGAACTGCATATTTCTCAGTGGTAACCAAGGTAAT
TTGGTCGGCCAGGCGCGCCGTGACAACTTCCTGCTGGGGGTTGCTGAAGCGCAGTTACGTAAATACAACCGCACTAATGT
GAACTTTCTTAGCCAAGGATGGGGGAATTGGGATCAGCAGGGCGGTTTGAAAGCTATGGAAGATATTATTGTGGCGCAGG
GCGATAAAATTAACTGTGTTTACAGTGAAATGGACGATATGGCGTTAGGTGCCATCCGTGCGCTGAAAGCGGCTAACAAG
CTGGATAATGTCAAAGTGTATGCTCACGACGGGTATAAAAAGGGGCTGGAAGCCGTGAAAAAAGGCGAATTGCAAGCGAC
GGCCTCGAATAACCCAGACTTGCTGACTCGCACGGTGCTAGAGGTTATTGGTCAATATCAGGCCGGTCAGACGAGTTTTC
CTGATTATGTCTATATCCCCTCCATTCTGATCACGAAAGATAACGTCGACAAATATTATAAAGAAGATTCGATTTTCTAA

Upstream 100 bases:

>100_bases
TAACAGCACCCGTTTTTGAAATAACGGCCGTAGTCAGTCAATCTGCCGAATTTAGTCTGATAACAACCCTTACCCGCCCT
GTATAACGAGAGGAAATATA

Downstream 100 bases:

>100_bases
CCGTCGTTTCCCCCTGTTTACAGGGGGATATGGGAGACCGACCAGGTGGATCAGTACGCTATTGAAGTCATACAGGTGAC
GAAAAGCTTCGGGGGGATTC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKKRLIGLLLAGVTISMSASAAEPLKIGFANRTLNGAFFNGLTEYMKIHAKEKGYTLITTDARGDLNKQIADVEDMLSQG
INYLILNPQDPQAGLRITEIAKRAGVPTVILDSDIALEAPVITRVQANNAKNNNLIGEYAVGQFGDKPMNCIFLSGNQGN
LVGQARRDNFLLGVAEAQLRKYNRTNVNFLSQGWGNWDQQGGLKAMEDIIVAQGDKINCVYSEMDDMALGAIRALKAANK
LDNVKVYAHDGYKKGLEAVKKGELQATASNNPDLLTRTVLEVIGQYQAGQTSFPDYVYIPSILITKDNVDKYYKEDSIF

Sequences:

>Translated_319_residues
MKKRLIGLLLAGVTISMSASAAEPLKIGFANRTLNGAFFNGLTEYMKIHAKEKGYTLITTDARGDLNKQIADVEDMLSQG
INYLILNPQDPQAGLRITEIAKRAGVPTVILDSDIALEAPVITRVQANNAKNNNLIGEYAVGQFGDKPMNCIFLSGNQGN
LVGQARRDNFLLGVAEAQLRKYNRTNVNFLSQGWGNWDQQGGLKAMEDIIVAQGDKINCVYSEMDDMALGAIRALKAANK
LDNVKVYAHDGYKKGLEAVKKGELQATASNNPDLLTRTVLEVIGQYQAGQTSFPDYVYIPSILITKDNVDKYYKEDSIF
>Mature_319_residues
MKKRLIGLLLAGVTISMSASAAEPLKIGFANRTLNGAFFNGLTEYMKIHAKEKGYTLITTDARGDLNKQIADVEDMLSQG
INYLILNPQDPQAGLRITEIAKRAGVPTVILDSDIALEAPVITRVQANNAKNNNLIGEYAVGQFGDKPMNCIFLSGNQGN
LVGQARRDNFLLGVAEAQLRKYNRTNVNFLSQGWGNWDQQGGLKAMEDIIVAQGDKINCVYSEMDDMALGAIRALKAANK
LDNVKVYAHDGYKKGLEAVKKGELQATASNNPDLLTRTVLEVIGQYQAGQTSFPDYVYIPSILITKDNVDKYYKEDSIF

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=275, Percent_Identity=29.8181818181818, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790674, Length=280, Percent_Identity=28.2142857142857, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790526, Length=314, Percent_Identity=23.8853503184713, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789990, Length=312, Percent_Identity=24.0384615384615, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34972; Mature: 34972

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLIGLLLAGVTISMSASAAEPLKIGFANRTLNGAFFNGLTEYMKIHAKEKGYTLITT
CCHHHHHHHHHCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
DARGDLNKQIADVEDMLSQGINYLILNPQDPQAGLRITEIAKRAGVPTVILDSDIALEAP
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCEECCC
VITRVQANNAKNNNLIGEYAVGQFGDKPMNCIFLSGNQGNLVGQARRDNFLLGVAEAQLR
EEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCEEECCCCCCEEEEEHHHHHH
KYNRTNVNFLSQGWGNWDQQGGLKAMEDIIVAQGDKINCVYSEMDDMALGAIRALKAANK
HCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
LDNVKVYAHDGYKKGLEAVKKGELQATASNNPDLLTRTVLEVIGQYQAGQTSFPDYVYIP
CCCEEEEEECCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEC
SILITKDNVDKYYKEDSIF
EEEEECCCHHHHHHHCCCC
>Mature Secondary Structure
MKKRLIGLLLAGVTISMSASAAEPLKIGFANRTLNGAFFNGLTEYMKIHAKEKGYTLITT
CCHHHHHHHHHCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
DARGDLNKQIADVEDMLSQGINYLILNPQDPQAGLRITEIAKRAGVPTVILDSDIALEAP
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCEECCC
VITRVQANNAKNNNLIGEYAVGQFGDKPMNCIFLSGNQGNLVGQARRDNFLLGVAEAQLR
EEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCEEECCCCCCEEEEEHHHHHH
KYNRTNVNFLSQGWGNWDQQGGLKAMEDIIVAQGDKINCVYSEMDDMALGAIRALKAANK
HCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
LDNVKVYAHDGYKKGLEAVKKGELQATASNNPDLLTRTVLEVIGQYQAGQTSFPDYVYIP
CCCEEEEEECCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEC
SILITKDNVDKYYKEDSIF
EEEEECCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]