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The map label for this gene is gntR [H]

Identifier: 170022395

GI number: 170022395

Start: 161125

End: 162063

Strand: Direct

Name: gntR [H]

Synonym: YPK_0134

Alternate gene names: 170022395

Gene position: 161125-162063 (Clockwise)

Preceding gene: 170022394

Following gene: 170022397

Centisome position: 3.44

GC content: 52.5

Gene sequence:

>939_bases
ATGACTGTCAGCCGTTATTTGCGGAATCCAGAACAGGTTTCTGCTGTTTTACAGGAAAAAATCGCGCTGGCGTTGGACGA
ATTGGGCTATATCCCCAACCGCGCTCCCGATATTCTCTCTAATGCCACCAGCCATGCCATTGGTGTGCTGCTGCCGTCGT
TAACTAATCAGGTTTTTGCTGAGGTTTTGCGGGGGATAGAGAGTGTGACGGATGCGCACGGTTACCAGACGATGCTGGCT
CACTACGGCTATCAGAAAGAGCGCGAAGAGCAGCGGCTGACGTCGCTTCTCTCCTACAATATTGATGGCCTCATCCTCTC
TGAACGCCACCATACCGCGCGGACCCTGAAAATGATTGAGGTCGCGGGTATCCCTGTTATTGAGCTAATGGATTGTATTT
CGCCTTGCCTTGATCTGGCCGTGGGGTTTAATAACTTTGAGGCTGCCCGGCAGATGACACAGCAAATTATCGCTAAAGGC
CATCGCTATGTGGTGTATTTTGGCGCTCGCCAAGATGAGCGCACGGTGATCAAGCAGCAGGGCTATGAGCAAGCGATGCG
CGAATCCGGTCTGGTACCGATGAGCGTGATGACCAGCCGTTCATCGTCATATTCCGCTGGCGCTGAACTGCTACGTGAAG
CGCAGGTCAGTTATCCACAAATCGACAGTATTTTCTGTACCAATGATGATTTAGCCATTGGTGCCGTATTTGAATGTCAG
CGTCAGGGGCTATCCATCCCCCATGACATGGCTATTGCCGGTTTCCATGGCCATGATATCGGCCAGTCGATGGTACCGAA
ACTGGCCAGCGTACTGACACCGAGGGAAAGAATGGGGCAAATCGGTGCCGAGCGTTTATTGGCCAGGTTGCGCGGAGAAG
TGGTAACACCCCAAATGGTGGATGTCGGCTTTACCATCCTCCCCGGTGGCAGTATATAA

Upstream 100 bases:

>100_bases
TGCTAGAATTGCCTCCCGTTCATCCAGCCTCAGTTCATTGATAATGAAGAAAAAAAGGCCAGTGCTACAAGATGTCGCCG
ATATGGTGGGAGTCACTAAA

Downstream 100 bases:

>100_bases
AAAATATAGCAATCATTTATATAGTAACTATTTACATAATAACCATTTATATAACAGCCATTTATATAACGACTATTTAT
CGCGCCGTTGGCTAAATAAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTVSRYLRNPEQVSAVLQEKIALALDELGYIPNRAPDILSNATSHAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLA
HYGYQKEREEQRLTSLLSYNIDGLILSERHHTARTLKMIEVAGIPVIELMDCISPCLDLAVGFNNFEAARQMTQQIIAKG
HRYVVYFGARQDERTVIKQQGYEQAMRESGLVPMSVMTSRSSSYSAGAELLREAQVSYPQIDSIFCTNDDLAIGAVFECQ
RQGLSIPHDMAIAGFHGHDIGQSMVPKLASVLTPRERMGQIGAERLLARLRGEVVTPQMVDVGFTILPGGSI

Sequences:

>Translated_312_residues
MTVSRYLRNPEQVSAVLQEKIALALDELGYIPNRAPDILSNATSHAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLA
HYGYQKEREEQRLTSLLSYNIDGLILSERHHTARTLKMIEVAGIPVIELMDCISPCLDLAVGFNNFEAARQMTQQIIAKG
HRYVVYFGARQDERTVIKQQGYEQAMRESGLVPMSVMTSRSSSYSAGAELLREAQVSYPQIDSIFCTNDDLAIGAVFECQ
RQGLSIPHDMAIAGFHGHDIGQSMVPKLASVLTPRERMGQIGAERLLARLRGEVVTPQMVDVGFTILPGGSI
>Mature_311_residues
TVSRYLRNPEQVSAVLQEKIALALDELGYIPNRAPDILSNATSHAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAH
YGYQKEREEQRLTSLLSYNIDGLILSERHHTARTLKMIEVAGIPVIELMDCISPCLDLAVGFNNFEAARQMTQQIIAKGH
RYVVYFGARQDERTVIKQQGYEQAMRESGLVPMSVMTSRSSSYSAGAELLREAQVSYPQIDSIFCTNDDLAIGAVFECQR
QGLSIPHDMAIAGFHGHDIGQSMVPKLASVLTPRERMGQIGAERLLARLRGEVVTPQMVDVGFTILPGGSI

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=78.525641025641, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=45.0479233226837, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1790369, Length=299, Percent_Identity=28.4280936454849, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=25.5520504731861, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788474, Length=302, Percent_Identity=24.5033112582781, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787906, Length=257, Percent_Identity=26.4591439688716, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787948, Length=306, Percent_Identity=24.1830065359477, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=24.7557003257329, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789202, Length=277, Percent_Identity=22.3826714801444, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786540, Length=301, Percent_Identity=23.9202657807309, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=23.3438485804416, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34405; Mature: 34274

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSRYLRNPEQVSAVLQEKIALALDELGYIPNRAPDILSNATSHAIGVLLPSLTNQVFA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EVLRGIESVTDAHGYQTMLAHYGYQKEREEQRLTSLLSYNIDGLILSERHHTARTLKMIE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
VAGIPVIELMDCISPCLDLAVGFNNFEAARQMTQQIIAKGHRYVVYFGARQDERTVIKQQ
HCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHC
GYEQAMRESGLVPMSVMTSRSSSYSAGAELLREAQVSYPQIDSIFCTNDDLAIGAVFECQ
CHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEHHHHHHH
RQGLSIPHDMAIAGFHGHDIGQSMVPKLASVLTPRERMGQIGAERLLARLRGEVVTPQMV
HCCCCCCCHHEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
DVGFTILPGGSI
HCCEEECCCCCC
>Mature Secondary Structure 
TVSRYLRNPEQVSAVLQEKIALALDELGYIPNRAPDILSNATSHAIGVLLPSLTNQVFA
CHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EVLRGIESVTDAHGYQTMLAHYGYQKEREEQRLTSLLSYNIDGLILSERHHTARTLKMIE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
VAGIPVIELMDCISPCLDLAVGFNNFEAARQMTQQIIAKGHRYVVYFGARQDERTVIKQQ
HCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHC
GYEQAMRESGLVPMSVMTSRSSSYSAGAELLREAQVSYPQIDSIFCTNDDLAIGAVFECQ
CHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEHHHHHHH
RQGLSIPHDMAIAGFHGHDIGQSMVPKLASVLTPRERMGQIGAERLLARLRGEVVTPQMV
HCCCCCCCHHEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
DVGFTILPGGSI
HCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]