Click here to switch to the map view.

The map label for this gene is rmlB [H]

Identifier: 169630859

GI number: 169630859

Start: 3834781

End: 3835776

Strand: Direct

Name: rmlB [H]

Synonym: MAB_3779

Alternate gene names: 169630859

Gene position: 3834781-3835776 (Clockwise)

Preceding gene: 169630858

Following gene: 169630860

Centisome position: 75.68

GC content: 62.65

Gene sequence:

>996_bases
ATGCGAATTTTGGTGACCGGCGGCGCCGGTTTCATCGGAGCCAACTTCGTACACGCGACAGTCCGCGAGCGCCCCGACGT
CTCGGTGACAGTCCTGGACGCGCTGACCTACGCGGGCAGCAGCGAATCACTGGCCCCCGTCGCCGCGGCGGTGCAGCTGG
TGCAGGGCGATATCTGCGATCCCGATACGGTGGAGCGGCTGGTCGCCGACAGTGACGTGGTGGTGCATTTCGCCGCCGAA
ACACATAACGACAATTCGCTGGCCGACCCGTCACCGTTTTTGCGCAGCAACATCATTGGCACCTACACGCTGCTCGAGGC
GGTGCGCCGCCATCAGGTGCGGCTGCACCACATTTCCACCGATGAGGTGTTCGGCGACCTTGAGCTCGATGACCCGAACC
GGTTCACGGAGACCACCCCGTACAACCCGTCCAGCCCGTACTCGGCCACCAAGGCCTCGGCCGACCTGCTGGTGCGCGCA
TGGATCCGGTCGTTCGGAATCAGCGCCACAATCTCCAACTGCTCCAACAACTATGGGCCGTACCAGCATGTGGAGAAGTT
CATTCCCCGGCAGATCACCAATATCCTGACCGGCCGCCGCCCACGCCTGTACGGGGCCGGTGCCAACGTACGGGACTGGA
TTCACGTCGACGACCACAACAGTGCGGTGTGGCGCATCATCGAGGCGGGCACCATCGGGCGCACGTATCTCATTGGTGCG
GACGGAGAACGAGACAACCTGTCGGTGCTGCACACCATTCTCGAGCTCATGGGCAAGTCCTCGGATGACTTCGACCATGT
CACCGATCGTGCCGGTCATGACCTGCGCTATGCCATTGACCCCACGCCGTTACGCGATGAATTGGGCTGGAAACCAACGC
ATAGCGACTTCACCGGAGGCCTGGCCGCCACCATCGACTGGTACCGGCGCAACGAAGAGTGGTGGCGCCCCATCAAGGAC
AACGTGGAATCTCAATACGCCGCGAGGGGACAGTGA

Upstream 100 bases:

>100_bases
CCGACCAGGTGCTGATTCAAGTACTCGGCGACAAGGGTTTGGTTGACACATTGACCTCGTTGGCGGACGCGCTCAGGTAG
ACGCCCACTAGGGTTATGCC

Downstream 100 bases:

>100_bases
TGAGCGTTTACGCGAAGACAGAGGGCAGTGATGAGCGTTTACACGAAGACAGAGGGCAGTGATGAGCGTTTACGCGAAGA
ACAGAGGGCACTGACGTGAA

Product: dTDP-glucose 4,6-dehydratase RmlB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRILVTGGAGFIGANFVHATVRERPDVSVTVLDALTYAGSSESLAPVAAAVQLVQGDICDPDTVERLVADSDVVVHFAAE
THNDNSLADPSPFLRSNIIGTYTLLEAVRRHQVRLHHISTDEVFGDLELDDPNRFTETTPYNPSSPYSATKASADLLVRA
WIRSFGISATISNCSNNYGPYQHVEKFIPRQITNILTGRRPRLYGAGANVRDWIHVDDHNSAVWRIIEAGTIGRTYLIGA
DGERDNLSVLHTILELMGKSSDDFDHVTDRAGHDLRYAIDPTPLRDELGWKPTHSDFTGGLAATIDWYRRNEEWWRPIKD
NVESQYAARGQ

Sequences:

>Translated_331_residues
MRILVTGGAGFIGANFVHATVRERPDVSVTVLDALTYAGSSESLAPVAAAVQLVQGDICDPDTVERLVADSDVVVHFAAE
THNDNSLADPSPFLRSNIIGTYTLLEAVRRHQVRLHHISTDEVFGDLELDDPNRFTETTPYNPSSPYSATKASADLLVRA
WIRSFGISATISNCSNNYGPYQHVEKFIPRQITNILTGRRPRLYGAGANVRDWIHVDDHNSAVWRIIEAGTIGRTYLIGA
DGERDNLSVLHTILELMGKSSDDFDHVTDRAGHDLRYAIDPTPLRDELGWKPTHSDFTGGLAATIDWYRRNEEWWRPIKD
NVESQYAARGQ
>Mature_331_residues
MRILVTGGAGFIGANFVHATVRERPDVSVTVLDALTYAGSSESLAPVAAAVQLVQGDICDPDTVERLVADSDVVVHFAAE
THNDNSLADPSPFLRSNIIGTYTLLEAVRRHQVRLHHISTDEVFGDLELDDPNRFTETTPYNPSSPYSATKASADLLVRA
WIRSFGISATISNCSNNYGPYQHVEKFIPRQITNILTGRRPRLYGAGANVRDWIHVDDHNSAVWRIIEAGTIGRTYLIGA
DGERDNLSVLHTILELMGKSSDDFDHVTDRAGHDLRYAIDPTPLRDELGWKPTHSDFTGGLAATIDWYRRNEEWWRPIKD
NVESQYAARGQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=32.0474777448071, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI42516563, Length=317, Percent_Identity=24.6056782334385, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=47.3837209302326, Blast_Score=310, Evalue=8e-86,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=43.646408839779, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1788366, Length=184, Percent_Identity=29.8913043478261, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=324, Percent_Identity=33.641975308642, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI115532424, Length=317, Percent_Identity=28.7066246056782, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=22.6586102719033, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=191, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=195, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=191, Percent_Identity=27.2251308900524, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=331, Percent_Identity=25.0755287009063, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356223, Length=319, Percent_Identity=23.5109717868339, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19923002, Length=326, Percent_Identity=28.2208588957055, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36778; Mature: 36778

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGAGFIGANFVHATVRERPDVSVTVLDALTYAGSSESLAPVAAAVQLVQGDICD
CEEEEECCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PDTVERLVADSDVVVHFAAETHNDNSLADPSPFLRSNIIGTYTLLEAVRRHQVRLHHIST
HHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECH
DEVFGDLELDDPNRFTETTPYNPSSPYSATKASADLLVRAWIRSFGISATISNCSNNYGP
HHHEECEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHCCCCCCC
YQHVEKFIPRQITNILTGRRPRLYGAGANVRDWIHVDDHNSAVWRIIEAGTIGRTYLIGA
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEEEECCCCCCEEEEEC
DGERDNLSVLHTILELMGKSSDDFDHVTDRAGHDLRYAIDPTPLRDELGWKPTHSDFTGG
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCCC
LAATIDWYRRNEEWWRPIKDNVESQYAARGQ
HHHHHHHHHCCHHHCCHHHHCHHHHHHCCCC
>Mature Secondary Structure
MRILVTGGAGFIGANFVHATVRERPDVSVTVLDALTYAGSSESLAPVAAAVQLVQGDICD
CEEEEECCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PDTVERLVADSDVVVHFAAETHNDNSLADPSPFLRSNIIGTYTLLEAVRRHQVRLHHIST
HHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECH
DEVFGDLELDDPNRFTETTPYNPSSPYSATKASADLLVRAWIRSFGISATISNCSNNYGP
HHHEECEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHCCCCCCC
YQHVEKFIPRQITNILTGRRPRLYGAGANVRDWIHVDDHNSAVWRIIEAGTIGRTYLIGA
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEEEECCCCCCEEEEEC
DGERDNLSVLHTILELMGKSSDDFDHVTDRAGHDLRYAIDPTPLRDELGWKPTHSDFTGG
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCCC
LAATIDWYRRNEEWWRPIKDNVESQYAARGQ
HHHHHHHHHCCHHHCCHHHHCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA