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The map label for this gene is punA [H]

Identifier: 169630739

GI number: 169630739

Start: 3713318

End: 3714187

Strand: Direct

Name: punA [H]

Synonym: MAB_3660

Alternate gene names: 169630739

Gene position: 3713318-3714187 (Clockwise)

Preceding gene: 169630736

Following gene: 169630740

Centisome position: 73.28

GC content: 69.66

Gene sequence:

>870_bases
GTGACCGATGAGGCCCCCGCACGCGGGAGGTACCCCCAGCCCGCGCGACGAGTCGACGACGCCGATGACCGGCTAGCTCC
CGACGATGCCGCAGCCCTGGCCGCCGCGGAGATCGCGACCCGTACCGGCGTCCCGGCCCATCGTGTCGCGGTGGTCCTCG
GATCCGGCTGGGCGCCGGCGGCCGGGGAACTAGGCACCCCCGCAACCACGATTCCCATGGCGGAGCTGCCCGCATTCAGC
CCACCGAGCGCGGCGGGGCACGGCGGCTCGGTGTTGTCGGTGCCGATCGGCGGTAGCGACGAGCGGATGCTGATCCTGCT
GGGCCGGATCCATGCGTACGAGGGCCATGATCTGCGTCATGTGGTGCATCCGGTGCGCACGGCCTGCGCTGCCGGCGCGC
GGACGATCATTCTCACCAACGCGGCGGGAGGTCTGCGCGAGGATTACGCGGTGGGCCAGCCGGTACTGATCAGCGACCAT
CTCAACCTGACGGCCCGATCACCCCTGGTGGGTGCACAGTTCGTCGACCTTGTCGACGCGTACTCTCCGCGACTTCGGGC
CTTGGCCCGGGAAATCGACGACAACCTCACCGAGGGGGTGTACGCAGGCCTACCGGGTCCGCACTACGAGACTCCCGCGG
AAATCCGCATGCTGCGCACGCTGGGCGCGGATTTGGTGGGCATGTCGACGGTGCAGGAAACCATCGCGGCGCGGGCGGCC
GGGGCCGAGGTGCTGGGGGTTTCCCTGGTGACCAACCTCGCGGCCGGAATGACCGGCGAATCCCTGAATCATGAGGAGGT
GCTGGCGGCGGGCCGAGCGTCGGCCACCCGCATGGGCCAACTTCTACGCGCGGTGATTGGTCGGCTATGA

Upstream 100 bases:

>100_bases
CATCGACGACCGCGCTGGCGGTCTCGGCGTGCGCGGTGGACGACGCTCCGGTGGTCAAGGGCATTAGATGAGTATCCCAT
CATGGTTAGGCTCCGTAGCT

Downstream 100 bases:

>100_bases
CTGTGGCAGCAGAAACGATCCAGGAGTGGATCGACCACGACCCGGATCCCCAGACCGTCGCCGAGCTGCGCGCGTGCTCC
GAAGACGAGCTTGCCCAGCG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: PNP; Inosine phosphorylase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTDEAPARGRYPQPARRVDDADDRLAPDDAAALAAAEIATRTGVPAHRVAVVLGSGWAPAAGELGTPATTIPMAELPAFS
PPSAAGHGGSVLSVPIGGSDERMLILLGRIHAYEGHDLRHVVHPVRTACAAGARTIILTNAAGGLREDYAVGQPVLISDH
LNLTARSPLVGAQFVDLVDAYSPRLRALAREIDDNLTEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVQETIAARAA
GAEVLGVSLVTNLAAGMTGESLNHEEVLAAGRASATRMGQLLRAVIGRL

Sequences:

>Translated_289_residues
MTDEAPARGRYPQPARRVDDADDRLAPDDAAALAAAEIATRTGVPAHRVAVVLGSGWAPAAGELGTPATTIPMAELPAFS
PPSAAGHGGSVLSVPIGGSDERMLILLGRIHAYEGHDLRHVVHPVRTACAAGARTIILTNAAGGLREDYAVGQPVLISDH
LNLTARSPLVGAQFVDLVDAYSPRLRALAREIDDNLTEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVQETIAARAA
GAEVLGVSLVTNLAAGMTGESLNHEEVLAAGRASATRMGQLLRAVIGRL
>Mature_288_residues
TDEAPARGRYPQPARRVDDADDRLAPDDAAALAAAEIATRTGVPAHRVAVVLGSGWAPAAGELGTPATTIPMAELPAFSP
PSAAGHGGSVLSVPIGGSDERMLILLGRIHAYEGHDLRHVVHPVRTACAAGARTIILTNAAGGLREDYAVGQPVLISDHL
NLTARSPLVGAQFVDLVDAYSPRLRALAREIDDNLTEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVQETIAARAAG
AEVLGVSLVTNLAAGMTGESLNHEEVLAAGRASATRMGQLLRAVIGRL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=259, Percent_Identity=39.7683397683398, Blast_Score=153, Evalue=1e-37,
Organism=Escherichia coli, GI1788746, Length=253, Percent_Identity=34.7826086956522, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17541190, Length=281, Percent_Identity=36.6548042704626, Blast_Score=150, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6323238, Length=292, Percent_Identity=33.9041095890411, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45552885, Length=259, Percent_Identity=33.2046332046332, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24656093, Length=259, Percent_Identity=33.2046332046332, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24656090, Length=259, Percent_Identity=33.2046332046332, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45552887, Length=259, Percent_Identity=33.2046332046332, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24762376, Length=246, Percent_Identity=29.2682926829268, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011269
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29922; Mature: 29791

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEAPARGRYPQPARRVDDADDRLAPDDAAALAAAEIATRTGVPAHRVAVVLGSGWAPA
CCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCC
AGELGTPATTIPMAELPAFSPPSAAGHGGSVLSVPIGGSDERMLILLGRIHAYEGHDLRH
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHHCCCCCCHHH
VVHPVRTACAAGARTIILTNAAGGLREDYAVGQPVLISDHLNLTARSPLVGAQFVDLVDA
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHH
YSPRLRALAREIDDNLTEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVQETIAARAA
CCHHHHHHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GAEVLGVSLVTNLAAGMTGESLNHEEVLAAGRASATRMGQLLRAVIGRL
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDEAPARGRYPQPARRVDDADDRLAPDDAAALAAAEIATRTGVPAHRVAVVLGSGWAPA
CCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCC
AGELGTPATTIPMAELPAFSPPSAAGHGGSVLSVPIGGSDERMLILLGRIHAYEGHDLRH
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEHHHCCCCCCHHH
VVHPVRTACAAGARTIILTNAAGGLREDYAVGQPVLISDHLNLTARSPLVGAQFVDLVDA
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHH
YSPRLRALAREIDDNLTEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVQETIAARAA
CCHHHHHHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GAEVLGVSLVTNLAAGMTGESLNHEEVLAAGRASATRMGQLLRAVIGRL
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]