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The map label for this gene is lcfB [H]

Identifier: 169630707

GI number: 169630707

Start: 3676217

End: 3677899

Strand: Reverse

Name: lcfB [H]

Synonym: MAB_3628c

Alternate gene names: 169630707

Gene position: 3677899-3676217 (Counterclockwise)

Preceding gene: 169630708

Following gene: 169630706

Centisome position: 72.58

GC content: 64.29

Gene sequence:

>1683_bases
ATGACCGCTGTGTCCGTTCCCCGCGCCGGCGTGCCCCGAGTCGACATCGCGGCCATGCTTCTGGATCGTGTTGGCGACAG
TCATCTGGGGCTACGCACCCGCGATCGCGACTGGACATGGGATCAGGTTGTCGATGAATCCGCCGCGCGGGGCGCGCTGG
CCCGGAAGCTGCGGGCGGACGGGCCCTTCCATATCGGCGTGCTGTTGGACAACGTGCCCGACTTCGTCTTTTGGCTGGGC
GGTGCCGCCCTCGCGGGGGCCACGATCGTGGGGATCAACCCAACCCGTGGTCCCGCGGAAATGGCCGCCGAGATCCGGCA
CACCGACTGTCAGCTGATCGTCACGGACACCGCGCATCTGGGGCGGCTACAGAGCCTTGAGCTAGGTCTGAGCGCGGATC
GTCTGCTGGTGATCGATAGCCCCGAATATCTGCAGCGCATTGCCGAGAGTCGCTGCACAGCACAGGCTTCCGAAGGCGTC
GGCGCGGATTCATTACTCCTGTTGCTGTTTACCTCAGGCACCACCGGGGCCTCCAAAGCGGTGAAGTGCAGTCAGGGGCG
GCTCGCTCAGATCGCTCACCTGGCCACCGAGAAGTTCGGGCATGTGCGCTCGGATGTCGACTATTGCTGCATGCCGTTGT
TCCACGGGAACGCGTTGATGGCGTTGTGGGCCCCGGCGCTGGCCAACGGCGCCACGGTGTGTTTGACGCCCACGTTCTCG
GCGTCACGTTTCTTACCCGATGTCCGGTACTTCGGCGCTACGTTTTTCACGTACGTCGGCAAGGCGCTGGGATACCTGTT
GGCCACGCCGGAGCGGGCCGATGACGCCGATAACCAGCTGGCGCGCGGCTTCGGCACCGAGGCCTCGCCCGAAGACCAGG
CCCAATTCCGGCGACGGTTCGGTGCGGAGCTGTTCGAGGGTTACGGTTCCAGCGAGGGTGGGGGAGCGGTGGTGCTAGAT
CCCGCCGCACCTCCGGGTGCGCTGGGGCGCCCCGCTCACGAGGGTGTGGTGGTGGTCGATCCTGAGACGTTGCAGGACTG
TGCCGCAGCGGTTTTCGATCAACACGGACGCGTGCTCAACGCCGACGCGGCTGTCGGTGAGATTGTCGACAAACAGGGTA
GACGCGGATTCGAGGGCTATTACAACAATGATGACGCCGATGCCGACCGCATCCGCAACGGGTGGTACTGGACGGGTGAT
CTCGGCTATCTCGATGCCGCGGGGTTCATCTACTTCGCGGGACGCCGTGGCGATTGGATTCGCGTCGACGGAGAGAACAT
GTCGGCACTCACCGTTGAGCGGGTACTGCGACGCCACCCGTTGGTGATCGCGGCGGGGGTGTACGCGGTTCCTGACCCGC
GTTCAGGTGACCAGGTGATGGCCTCCATCGAGGTGGCCGAGCCGGATTCGTTCGATGTGGATGGGTTCACCCAGTACCTC
TCGGCGCAAGAAGAACTGGGCAGCAAGGGCATTCCGCGGTTCCTGCGGATATCGGCCAACTTGCCGGTCACCGGCTCGAA
CAAGGTGCTCAAGCGCGAACTGCAGGAGCAGCGCTGGCATACCGACGAACCCGTCTTCCGCTGGGTGGGAAGGGGCGGCC
CGGTTTTCCTGCGGATGAGTTCAGATGACAAAACCGCACTCGATGCTCAGTTCGCGGTGTATGGACGACAACGGCTGCTC
TAG

Upstream 100 bases:

>100_bases
GCGAGGACTCATATCACTCGGGACCGGTGAGTATCACCAAGTTGGTTCCGGAGGAGCTGCCCCAGGAGTATCGGGATCTG
AAGGGTGCATCATCGGCGCT

Downstream 100 bases:

>100_bases
AGCGCAGTATGGAACCAGTCAGCGAAAGGACGGCCCCCATGACTGACGGCGCGATTCGTGAAATCGACAGCGGCGCACCG
ATGACTAGGTTTGCCCGGGG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTAVSVPRAGVPRVDIAAMLLDRVGDSHLGLRTRDRDWTWDQVVDESAARGALARKLRADGPFHIGVLLDNVPDFVFWLG
GAALAGATIVGINPTRGPAEMAAEIRHTDCQLIVTDTAHLGRLQSLELGLSADRLLVIDSPEYLQRIAESRCTAQASEGV
GADSLLLLLFTSGTTGASKAVKCSQGRLAQIAHLATEKFGHVRSDVDYCCMPLFHGNALMALWAPALANGATVCLTPTFS
ASRFLPDVRYFGATFFTYVGKALGYLLATPERADDADNQLARGFGTEASPEDQAQFRRRFGAELFEGYGSSEGGGAVVLD
PAAPPGALGRPAHEGVVVVDPETLQDCAAAVFDQHGRVLNADAAVGEIVDKQGRRGFEGYYNNDDADADRIRNGWYWTGD
LGYLDAAGFIYFAGRRGDWIRVDGENMSALTVERVLRRHPLVIAAGVYAVPDPRSGDQVMASIEVAEPDSFDVDGFTQYL
SAQEELGSKGIPRFLRISANLPVTGSNKVLKRELQEQRWHTDEPVFRWVGRGGPVFLRMSSDDKTALDAQFAVYGRQRLL

Sequences:

>Translated_560_residues
MTAVSVPRAGVPRVDIAAMLLDRVGDSHLGLRTRDRDWTWDQVVDESAARGALARKLRADGPFHIGVLLDNVPDFVFWLG
GAALAGATIVGINPTRGPAEMAAEIRHTDCQLIVTDTAHLGRLQSLELGLSADRLLVIDSPEYLQRIAESRCTAQASEGV
GADSLLLLLFTSGTTGASKAVKCSQGRLAQIAHLATEKFGHVRSDVDYCCMPLFHGNALMALWAPALANGATVCLTPTFS
ASRFLPDVRYFGATFFTYVGKALGYLLATPERADDADNQLARGFGTEASPEDQAQFRRRFGAELFEGYGSSEGGGAVVLD
PAAPPGALGRPAHEGVVVVDPETLQDCAAAVFDQHGRVLNADAAVGEIVDKQGRRGFEGYYNNDDADADRIRNGWYWTGD
LGYLDAAGFIYFAGRRGDWIRVDGENMSALTVERVLRRHPLVIAAGVYAVPDPRSGDQVMASIEVAEPDSFDVDGFTQYL
SAQEELGSKGIPRFLRISANLPVTGSNKVLKRELQEQRWHTDEPVFRWVGRGGPVFLRMSSDDKTALDAQFAVYGRQRLL
>Mature_559_residues
TAVSVPRAGVPRVDIAAMLLDRVGDSHLGLRTRDRDWTWDQVVDESAARGALARKLRADGPFHIGVLLDNVPDFVFWLGG
AALAGATIVGINPTRGPAEMAAEIRHTDCQLIVTDTAHLGRLQSLELGLSADRLLVIDSPEYLQRIAESRCTAQASEGVG
ADSLLLLLFTSGTTGASKAVKCSQGRLAQIAHLATEKFGHVRSDVDYCCMPLFHGNALMALWAPALANGATVCLTPTFSA
SRFLPDVRYFGATFFTYVGKALGYLLATPERADDADNQLARGFGTEASPEDQAQFRRRFGAELFEGYGSSEGGGAVVLDP
AAPPGALGRPAHEGVVVVDPETLQDCAAAVFDQHGRVLNADAAVGEIVDKQGRRGFEGYYNNDDADADRIRNGWYWTGDL
GYLDAAGFIYFAGRRGDWIRVDGENMSALTVERVLRRHPLVIAAGVYAVPDPRSGDQVMASIEVAEPDSFDVDGFTQYLS
AQEELGSKGIPRFLRISANLPVTGSNKVLKRELQEQRWHTDEPVFRWVGRGGPVFLRMSSDDKTALDAQFAVYGRQRLL

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13325057, Length=471, Percent_Identity=29.0870488322718, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI38524616, Length=516, Percent_Identity=28.1007751937984, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI227499619, Length=400, Percent_Identity=28.5, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI40807357, Length=530, Percent_Identity=27.7358490566038, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI62865631, Length=493, Percent_Identity=26.369168356998, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI13325055, Length=493, Percent_Identity=26.369168356998, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI13236579, Length=369, Percent_Identity=27.6422764227642, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI227499621, Length=339, Percent_Identity=27.7286135693215, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI115511026, Length=477, Percent_Identity=24.9475890985325, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI156151445, Length=533, Percent_Identity=22.8893058161351, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI122937307, Length=244, Percent_Identity=29.5081967213115, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI38505220, Length=433, Percent_Identity=26.0969976905312, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI42544132, Length=249, Percent_Identity=27.3092369477912, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI58082049, Length=478, Percent_Identity=23.4309623430962, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI157311624, Length=482, Percent_Identity=23.4439834024896, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI157311622, Length=482, Percent_Identity=23.4439834024896, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI28416953, Length=386, Percent_Identity=25.1295336787565, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI8923896, Length=380, Percent_Identity=25.5263157894737, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI253970489, Length=380, Percent_Identity=25.5263157894737, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI221142682, Length=469, Percent_Identity=26.6524520255864, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788107, Length=497, Percent_Identity=23.3400402414487, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI145693145, Length=395, Percent_Identity=27.0886075949367, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786810, Length=376, Percent_Identity=23.936170212766, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790505, Length=394, Percent_Identity=22.0812182741117, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115534440, Length=493, Percent_Identity=27.5862068965517, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17551278, Length=501, Percent_Identity=27.7445109780439, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=436, Percent_Identity=24.7706422018349, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=423, Percent_Identity=21.9858156028369, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=356, Percent_Identity=25, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319515, Length=418, Percent_Identity=26.7942583732057, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45552801, Length=547, Percent_Identity=26.691042047532, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550496, Length=542, Percent_Identity=26.9372693726937, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24583465, Length=521, Percent_Identity=27.063339731286, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24583463, Length=521, Percent_Identity=27.063339731286, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281364798, Length=521, Percent_Identity=27.063339731286, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI18859661, Length=513, Percent_Identity=23.9766081871345, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI20130353, Length=392, Percent_Identity=27.8061224489796, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28573631, Length=392, Percent_Identity=27.8061224489796, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24667959, Length=438, Percent_Identity=25.5707762557078, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24667955, Length=438, Percent_Identity=25.5707762557078, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI62472339, Length=438, Percent_Identity=25.5707762557078, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653035, Length=481, Percent_Identity=23.4927234927235, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24581924, Length=540, Percent_Identity=24.8148148148148, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648260, Length=364, Percent_Identity=24.7252747252747, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161076582, Length=405, Percent_Identity=25.1851851851852, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281365686, Length=326, Percent_Identity=26.0736196319018, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=309, Percent_Identity=24.5954692556634, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24656500, Length=511, Percent_Identity=23.4833659491194, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648257, Length=453, Percent_Identity=22.075055187638, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355181, Length=305, Percent_Identity=24.5901639344262, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648253, Length=310, Percent_Identity=21.9354838709677, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 60644; Mature: 60513

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVSVPRAGVPRVDIAAMLLDRVGDSHLGLRTRDRDWTWDQVVDESAARGALARKLRAD
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC
GPFHIGVLLDNVPDFVFWLGGAALAGATIVGINPTRGPAEMAAEIRHTDCQLIVTDTAHL
CCEEEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHH
GRLQSLELGLSADRLLVIDSPEYLQRIAESRCTAQASEGVGADSLLLLLFTSGTTGASKA
CCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
VKCSQGRLAQIAHLATEKFGHVRSDVDYCCMPLFHGNALMALWAPALANGATVCLTPTFS
EECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHCCCCEEEEECCCC
ASRFLPDVRYFGATFFTYVGKALGYLLATPERADDADNQLARGFGTEASPEDQAQFRRRF
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
GAELFEGYGSSEGGGAVVLDPAAPPGALGRPAHEGVVVVDPETLQDCAAAVFDQHGRVLN
CHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEE
ADAAVGEIVDKQGRRGFEGYYNNDDADADRIRNGWYWTGDLGYLDAAGFIYFAGRRGDWI
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCCEEEEECCCCCEE
RVDGENMSALTVERVLRRHPLVIAAGVYAVPDPRSGDQVMASIEVAEPDSFDVDGFTQYL
EECCCCCHHHHHHHHHHHCCEEEEECEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
SAQEELGSKGIPRFLRISANLPVTGSNKVLKRELQEQRWHTDEPVFRWVGRGGPVFLRMS
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEC
SDDKTALDAQFAVYGRQRLL
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAVSVPRAGVPRVDIAAMLLDRVGDSHLGLRTRDRDWTWDQVVDESAARGALARKLRAD
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC
GPFHIGVLLDNVPDFVFWLGGAALAGATIVGINPTRGPAEMAAEIRHTDCQLIVTDTAHL
CCEEEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHH
GRLQSLELGLSADRLLVIDSPEYLQRIAESRCTAQASEGVGADSLLLLLFTSGTTGASKA
CCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
VKCSQGRLAQIAHLATEKFGHVRSDVDYCCMPLFHGNALMALWAPALANGATVCLTPTFS
EECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHCCCCEEEEECCCC
ASRFLPDVRYFGATFFTYVGKALGYLLATPERADDADNQLARGFGTEASPEDQAQFRRRF
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
GAELFEGYGSSEGGGAVVLDPAAPPGALGRPAHEGVVVVDPETLQDCAAAVFDQHGRVLN
CHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEE
ADAAVGEIVDKQGRRGFEGYYNNDDADADRIRNGWYWTGDLGYLDAAGFIYFAGRRGDWI
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCCEEEEECCCCCEE
RVDGENMSALTVERVLRRHPLVIAAGVYAVPDPRSGDQVMASIEVAEPDSFDVDGFTQYL
EECCCCCHHHHHHHHHHHCCEEEEECEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
SAQEELGSKGIPRFLRISANLPVTGSNKVLKRELQEQRWHTDEPVFRWVGRGGPVFLRMS
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEC
SDDKTALDAQFAVYGRQRLL
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]