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The map label for this gene is kshA [H]

Identifier: 169630706

GI number: 169630706

Start: 3675039

End: 3676178

Strand: Reverse

Name: kshA [H]

Synonym: MAB_3627c

Alternate gene names: 169630706

Gene position: 3676178-3675039 (Counterclockwise)

Preceding gene: 169630707

Following gene: 169630705

Centisome position: 72.55

GC content: 59.82

Gene sequence:

>1140_bases
ATGACTGACGGCGCGATTCGTGAAATCGACAGCGGCGCACCGATGACTAGGTTTGCCCGGGGGTGGCACTGCCTGGGTCT
GGCCGAGACCTTCCTTGACGGGCAACCGCACGGTATCGAGGCGTTTGGCACCAAGTTGGTGGTGTTCGCCGATTCGCATG
GCGATATCAAGGTGCTGGACGGTTATTGCCGGCATATGGGAGGCGACCTGTCGCAGGGCACCGTCAAGGACGACACCGTG
GCCTGTCCTTTTCATGATTGGCGGTGGGGCGGCGACGGTAGGTGCACATTGGTGCCGTACGCCAAGCGCACCCCGAAGCT
CGCCCGTACCCGCAGCTGGCCCACGCTCGAGGTAAACGGTCAACTCCTGGTGTGGCACGACCCCGAAGGTTCCGCGCCAC
CGGCCGAGCTCACCCCGCCGACCATCGAGGGTTACGGGGAGGGTAAATGGTCTCCCTGGCAATGGAATTCGATTCTCATC
GAGGGATCACACTGCCGGGAGATCGTCGACAACAACGTGGATATGGCGCACTTCTTCTACATCCATCACGCGTATCCGAC
GTATTTCAAGAATGTGATCGAGGGGCAGACCGCCAGCCAGTTCATGGAATCGAAGCCGCGTCCGGACTATGTTCCGAATC
CCGAGAAACTCTGGGAAGGAACCGGTGTGCGCTCGGAGGCCACATATTTTGGGCCCGCGTACATGATCGACTGGATTCAC
AACGACCTCGGCCCCGGATTCACTGTCGAGGTGGCACTCATCAATTGCCACTATCCGGTGACACACGACTCCTTTGTCTT
GCAGTGGGGTGTCGCCATCCAGGAGATGCCCGACCTGCCGCCCGATAACGCGCGCAAGCTCGCTGCCGCGATGGCCAAGT
CGTTCGGGGATGGGTTCCTCGAAGATGTCGAGATCTGGAAGAACAAAACGCGTATCGACAATCCCTTGCTGACCCACGAG
GACGGCGCCGTGTACCAGCATCGTCGTTGGTATGAGCAGTTCTACGTGGATCTGGCCGATGTCACTTCTGATATGACTGA
GCGATTCGAGCTTGAGGTGGATACCACACACGCCCATGGTGTGTGGGTGCACGAGGTCGAGGAGAACCTGGTGGAGCATG
CTGCCACCGCGTCGCACTGA

Upstream 100 bases:

>100_bases
CAGATGACAAAACCGCACTCGATGCTCAGTTCGCGGTGTATGGACGACAACGGCTGCTCTAGAGCGCAGTATGGAACCAG
TCAGCGAAAGGACGGCCCCC

Downstream 100 bases:

>100_bases
CCAGCCTTAGGCTGGGGCCCGTGACTTCGGACAGATTGACTCTCACCGACAGCCCCGGCGGCCACTGGCGACGCATCGAC
GTCGTTGAGGAAACCGGATC

Product: oxidoreductase

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTDGAIREIDSGAPMTRFARGWHCLGLAETFLDGQPHGIEAFGTKLVVFADSHGDIKVLDGYCRHMGGDLSQGTVKDDTV
ACPFHDWRWGGDGRCTLVPYAKRTPKLARTRSWPTLEVNGQLLVWHDPEGSAPPAELTPPTIEGYGEGKWSPWQWNSILI
EGSHCREIVDNNVDMAHFFYIHHAYPTYFKNVIEGQTASQFMESKPRPDYVPNPEKLWEGTGVRSEATYFGPAYMIDWIH
NDLGPGFTVEVALINCHYPVTHDSFVLQWGVAIQEMPDLPPDNARKLAAAMAKSFGDGFLEDVEIWKNKTRIDNPLLTHE
DGAVYQHRRWYEQFYVDLADVTSDMTERFELEVDTTHAHGVWVHEVEENLVEHAATASH

Sequences:

>Translated_379_residues
MTDGAIREIDSGAPMTRFARGWHCLGLAETFLDGQPHGIEAFGTKLVVFADSHGDIKVLDGYCRHMGGDLSQGTVKDDTV
ACPFHDWRWGGDGRCTLVPYAKRTPKLARTRSWPTLEVNGQLLVWHDPEGSAPPAELTPPTIEGYGEGKWSPWQWNSILI
EGSHCREIVDNNVDMAHFFYIHHAYPTYFKNVIEGQTASQFMESKPRPDYVPNPEKLWEGTGVRSEATYFGPAYMIDWIH
NDLGPGFTVEVALINCHYPVTHDSFVLQWGVAIQEMPDLPPDNARKLAAAMAKSFGDGFLEDVEIWKNKTRIDNPLLTHE
DGAVYQHRRWYEQFYVDLADVTSDMTERFELEVDTTHAHGVWVHEVEENLVEHAATASH
>Mature_378_residues
TDGAIREIDSGAPMTRFARGWHCLGLAETFLDGQPHGIEAFGTKLVVFADSHGDIKVLDGYCRHMGGDLSQGTVKDDTVA
CPFHDWRWGGDGRCTLVPYAKRTPKLARTRSWPTLEVNGQLLVWHDPEGSAPPAELTPPTIEGYGEGKWSPWQWNSILIE
GSHCREIVDNNVDMAHFFYIHHAYPTYFKNVIEGQTASQFMESKPRPDYVPNPEKLWEGTGVRSEATYFGPAYMIDWIHN
DLGPGFTVEVALINCHYPVTHDSFVLQWGVAIQEMPDLPPDNARKLAAAMAKSFGDGFLEDVEIWKNKTRIDNPLLTHED
GAVYQHRRWYEQFYVDLADVTSDMTERFELEVDTTHAHGVWVHEVEENLVEHAATASH

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=359, Percent_Identity=27.2980501392758, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161085896, Length=347, Percent_Identity=26.8011527377522, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: NA

Molecular weight: Translated: 42821; Mature: 42690

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGAIREIDSGAPMTRFARGWHCLGLAETFLDGQPHGIEAFGTKLVVFADSHGDIKVLD
CCCCCHHHCCCCCCHHHHHCCEEEEHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEC
GYCRHMGGDLSQGTVKDDTVACPFHDWRWGGDGRCTLVPYAKRTPKLARTRSWPTLEVNG
HHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCEEEECC
QLLVWHDPEGSAPPAELTPPTIEGYGEGKWSPWQWNSILIEGSHCREIVDNNVDMAHFFY
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEECHHHHHHHHCCCCEEEEEE
IHHAYPTYFKNVIEGQTASQFMESKPRPDYVPNPEKLWEGTGVRSEATYFGPAYMIDWIH
EECCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEECCHHHHHHHHH
NDLGPGFTVEVALINCHYPVTHDSFVLQWGVAIQEMPDLPPDNARKLAAAMAKSFGDGFL
HCCCCCEEEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
EDVEIWKNKTRIDNPLLTHEDGAVYQHRRWYEQFYVDLADVTSDMTERFELEVDTTHAHG
HHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
VWVHEVEENLVEHAATASH
EEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDGAIREIDSGAPMTRFARGWHCLGLAETFLDGQPHGIEAFGTKLVVFADSHGDIKVLD
CCCCHHHCCCCCCHHHHHCCEEEEHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEC
GYCRHMGGDLSQGTVKDDTVACPFHDWRWGGDGRCTLVPYAKRTPKLARTRSWPTLEVNG
HHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCEEEECC
QLLVWHDPEGSAPPAELTPPTIEGYGEGKWSPWQWNSILIEGSHCREIVDNNVDMAHFFY
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEECHHHHHHHHCCCCEEEEEE
IHHAYPTYFKNVIEGQTASQFMESKPRPDYVPNPEKLWEGTGVRSEATYFGPAYMIDWIH
EECCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEECCHHHHHHHHH
NDLGPGFTVEVALINCHYPVTHDSFVLQWGVAIQEMPDLPPDNARKLAAAMAKSFGDGFL
HCCCCCEEEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
EDVEIWKNKTRIDNPLLTHEDGAVYQHRRWYEQFYVDLADVTSDMTERFELEVDTTHAHG
HHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
VWVHEVEENLVEHAATASH
EEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]