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The map label for this gene is birA [H]

Identifier: 169630705

GI number: 169630705

Start: 3674224

End: 3675018

Strand: Reverse

Name: birA [H]

Synonym: MAB_3626c

Alternate gene names: 169630705

Gene position: 3675018-3674224 (Counterclockwise)

Preceding gene: 169630706

Following gene: 169630704

Centisome position: 72.53

GC content: 68.93

Gene sequence:

>795_bases
GTGACTTCGGACAGATTGACTCTCACCGACAGCCCCGGCGGCCACTGGCGACGCATCGACGTCGTTGAGGAAACCGGATC
TACCAATGCCGATCTGGTGACGCGCGCCGCGGCGGGGGAGGACATCGACGGCGTCGTGCTGCTCGCCGAGCATCAGACCG
CAGGGCGTGGACGCCACGGCCGTTCCTGGGGCGCCCCCGCCCACACGCAGCTGTCCATGTCGGTGGGTATCGGAGTGGGC
GAGGTGCCGCCGGACCGGTGGGGGCTGGTGCCGCTCTTGGCCGGAGTGGCGATTGTCGATGCCGTCGCCGAGGTCAGTGG
AATCCAGGCCGGGCTCAAGTGGCCCAATGATGTGCTGGTCGATGGGCGCAAGTTGTGCGGAATTCTCGCTGAGGTCGCCA
CGCCACACCAGGCCATCGTGCTCGGGCTCGGGCTCAATGCCACTTTTGCCCAGCACGAGCTGCCCGATCCCAATGCAACA
TCCCTGACGATTCTCGGCTGGGCCGACCCGGACCGCGACGAGTTGGCGCGTGCGGTACTGCGTGAGCTCGGCGGCCGCAT
CGAGCACTGGCGTGCCGCGCGCGGGCTGGACGAGCGGCTGCTGGAGGACTACCGCGAGCGCAGTGTCACCCTGGGGTCGC
GGGTGCGTGCCGAACTGCCGGGTGAGCGCGAACTGGTCGGGGTCGCGGTCGGACTGGGCAACGACGGGCAGCTGCGCATC
GAGGCTGCTGACGGCACGGTCACCGCGGTCACGGCGGGCGATATTACGCATCTGCGCCCGCTCGGCGACCAGTAA

Upstream 100 bases:

>100_bases
ATACCACACACGCCCATGGTGTGTGGGTGCACGAGGTCGAGGAGAACCTGGTGGAGCATGCTGCCACCGCGTCGCACTGA
CCAGCCTTAGGCTGGGGCCC

Downstream 100 bases:

>100_bases
GGTCGCTGCACGTGGGGTATCCGGAAAATGTGCTCGCCGATGACGAGCAGGTGGTGCTGCACCGCCATCCACACTGGAAG
CGGTTGATCGGCCCGGCGCT

Product: biotin--acetyl-CoA-carboxylase ligase (BirA bifunctional protein)

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTSDRLTLTDSPGGHWRRIDVVEETGSTNADLVTRAAAGEDIDGVVLLAEHQTAGRGRHGRSWGAPAHTQLSMSVGIGVG
EVPPDRWGLVPLLAGVAIVDAVAEVSGIQAGLKWPNDVLVDGRKLCGILAEVATPHQAIVLGLGLNATFAQHELPDPNAT
SLTILGWADPDRDELARAVLRELGGRIEHWRAARGLDERLLEDYRERSVTLGSRVRAELPGERELVGVAVGLGNDGQLRI
EAADGTVTAVTAGDITHLRPLGDQ

Sequences:

>Translated_264_residues
MTSDRLTLTDSPGGHWRRIDVVEETGSTNADLVTRAAAGEDIDGVVLLAEHQTAGRGRHGRSWGAPAHTQLSMSVGIGVG
EVPPDRWGLVPLLAGVAIVDAVAEVSGIQAGLKWPNDVLVDGRKLCGILAEVATPHQAIVLGLGLNATFAQHELPDPNAT
SLTILGWADPDRDELARAVLRELGGRIEHWRAARGLDERLLEDYRERSVTLGSRVRAELPGERELVGVAVGLGNDGQLRI
EAADGTVTAVTAGDITHLRPLGDQ
>Mature_263_residues
TSDRLTLTDSPGGHWRRIDVVEETGSTNADLVTRAAAGEDIDGVVLLAEHQTAGRGRHGRSWGAPAHTQLSMSVGIGVGE
VPPDRWGLVPLLAGVAIVDAVAEVSGIQAGLKWPNDVLVDGRKLCGILAEVATPHQAIVLGLGLNATFAQHELPDPNATS
LTILGWADPDRDELARAVLRELGGRIEHWRAARGLDERLLEDYRERSVTLGSRVRAELPGERELVGVAVGLGNDGQLRIE
AADGTVTAVTAGDITHLRPLGDQ

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=239, Percent_Identity=30.5439330543933, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27965; Mature: 27834

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDRLTLTDSPGGHWRRIDVVEETGSTNADLVTRAAAGEDIDGVVLLAEHQTAGRGRHG
CCCCCEEEECCCCCCEEEEEEEECCCCCCHHHEEECCCCCCCCCEEEEEECCCCCCCCCC
RSWGAPAHTQLSMSVGIGVGEVPPDRWGLVPLLAGVAIVDAVAEVSGIQAGLKWPNDVLV
CCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
DGRKLCGILAEVATPHQAIVLGLGLNATFAQHELPDPNATSLTILGWADPDRDELARAVL
CCHHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
RELGGRIEHWRAARGLDERLLEDYRERSVTLGSRVRAELPGERELVGVAVGLGNDGQLRI
HHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCCEEEE
EAADGTVTAVTAGDITHLRPLGDQ
EECCCEEEEEECCCCEECCCCCCC
>Mature Secondary Structure 
TSDRLTLTDSPGGHWRRIDVVEETGSTNADLVTRAAAGEDIDGVVLLAEHQTAGRGRHG
CCCCEEEECCCCCCEEEEEEEECCCCCCHHHEEECCCCCCCCCEEEEEECCCCCCCCCC
RSWGAPAHTQLSMSVGIGVGEVPPDRWGLVPLLAGVAIVDAVAEVSGIQAGLKWPNDVLV
CCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
DGRKLCGILAEVATPHQAIVLGLGLNATFAQHELPDPNATSLTILGWADPDRDELARAVL
CCHHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
RELGGRIEHWRAARGLDERLLEDYRERSVTLGSRVRAELPGERELVGVAVGLGNDGQLRI
HHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCCEEEE
EAADGTVTAVTAGDITHLRPLGDQ
EECCCEEEEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]