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The map label for this gene is menE [H]

Identifier: 169630672

GI number: 169630672

Start: 3641838

End: 3643367

Strand: Reverse

Name: menE [H]

Synonym: MAB_3592c

Alternate gene names: 169630672

Gene position: 3643367-3641838 (Counterclockwise)

Preceding gene: 169630674

Following gene: 169630671

Centisome position: 71.9

GC content: 64.31

Gene sequence:

>1530_bases
GTGTTCTTCGATCTGAGCGTGCGAGATTTCCTGGACCGAGCCGAACTTGTGTACCCCGAACGGGTGGGTGTCGTCGATGA
GCCGGTGCAACCGGCCCCGTCCTGGGGGCAGTTGACCTACCGGCAACTCGCCGCGCGAGCACGTGGACAGGCGGCCGCTC
TCGACGCCCTCGGGGTCGGGCCGGGGGAGCGGGTGGCGATCGTTTCGCAGAACAGCGCGCGGCTGCTCACCTCGTTCTTC
GGCGTATCCGGGTGGGGACGCATCTTGGTGCCCATAAACTTTCGGTTGGCGCCCGCCGAGATCGAGTACATCGTCGGACA
TTCGGGTGCTTCGGTGCTGGTGGTCGACCCCGAGGTGCGGGGCCTGCTGGACACCGTACGGGCCACCCACAACTTTGTGC
TGGGCGAGGACGATGCGGCAGTATTCGGGGAGCTGGATTCGCCTCACGCTGTGACCCCCGATCCATGGTCCGGGTCCGAA
ACAGGCACTGCGACTCTCAATTACACCTCGGGCACAACGAGCCGCCCCAAGGGTGTTCAGCTCACTCATCGCAATATCTG
GATCAACGCCGTTGTGTTCGGGCTGCATGCGACCCTGCGCGATGACGATGTGCTGCTGCATACATTGCCGATGTTTCACT
GCAACGGGTGGGGCTGGCCCTACGCCGCGACGGGTATGGGAGCGCGGCACATTGTGCTGCGCAAGGTCGACGGTGACGAG
ATTCTGCGCCGTGTCGAACAACACGGGGTCACCGTGATGTGTGCGGCCCCAACGGTGGTCGACTCGATACTGGATGCCGC
CGCACGTTGGCGGGGGCCCATTCCCGGGCGTGATCGCGTTCGCATCATCGTCGCCGGAGCGCCCCCTCCGACGCGGACCA
TCGCACGGGTGCGCACCGAGCTGGGGTGGGAGTTCATCCAGATCTATGGGCTTACCGAGACGGCGCCGCTGATCACGATG
AATCGTTTCCGTTCCGAGTGGGCGGGTCTCGATGTGCAGGAGCAGGCCCGCATGCTGGGCCGCGCGGGTGCCCCAGCGAT
CGGTGTGCGCGTATCCGTCGCCGAGGACGGTGAGGTGCTGACCCAGTCGAACACGAACCTGGACGGATATTGGGATCAGC
CGCAGGCCACTGCGGAGGCATTGGCGGGCAATTGGTTTCATACCGGTGATCGCGGATCGGTGGACGACGGATACCTGACC
ATCTCGGACCGCAAAAAGGACATCATCATTACCGGTGGGGAGAATGTCACCTCCATCGAGGTGGAGGACGCACTGATATC
GCATCCCGGCGTGCGCGAGGCCGCCGTGGTCGGTGTTCCCGACGAGAAATGGGGCGAGTTGGTGACCGCGGTAGTGGTGG
TCGTCACGCTGGACGATCCGCCATCGGGCGCCGATCTGATAGCGCACTGCCGGGAGAGACTCGCGGGATACAAATGCCCG
AAACGCGTCGAGTTCGTCGACTCCCTGCCCAGAACGGCCACCGGGAAAGTCCAGAAGTTCAGGCTTCGAGAGCAGCTCGC
GCGAGACTAG

Upstream 100 bases:

>100_bases
AGCGCCATCATTAAACACCCCGGTGCCAGTGACCGGCAAGATGAACCGTTGCTTCGTGGATATCCCCCCTGCACCGCGTG
TCAGGCGGCAGGATTGGTGC

Downstream 100 bases:

>100_bases
TTCGCAACTCGCCCCTTGGGTACCCCGGTTTTGTCACGATCTAGTGACACGATGGGATCTATGAGGCAAAGGATCCTTGT
TGTCGACGATGACGCGTCAT

Product: putative medium-chain acyl-CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MFFDLSVRDFLDRAELVYPERVGVVDEPVQPAPSWGQLTYRQLAARARGQAAALDALGVGPGERVAIVSQNSARLLTSFF
GVSGWGRILVPINFRLAPAEIEYIVGHSGASVLVVDPEVRGLLDTVRATHNFVLGEDDAAVFGELDSPHAVTPDPWSGSE
TGTATLNYTSGTTSRPKGVQLTHRNIWINAVVFGLHATLRDDDVLLHTLPMFHCNGWGWPYAATGMGARHIVLRKVDGDE
ILRRVEQHGVTVMCAAPTVVDSILDAAARWRGPIPGRDRVRIIVAGAPPPTRTIARVRTELGWEFIQIYGLTETAPLITM
NRFRSEWAGLDVQEQARMLGRAGAPAIGVRVSVAEDGEVLTQSNTNLDGYWDQPQATAEALAGNWFHTGDRGSVDDGYLT
ISDRKKDIIITGGENVTSIEVEDALISHPGVREAAVVGVPDEKWGELVTAVVVVVTLDDPPSGADLIAHCRERLAGYKCP
KRVEFVDSLPRTATGKVQKFRLREQLARD

Sequences:

>Translated_509_residues
MFFDLSVRDFLDRAELVYPERVGVVDEPVQPAPSWGQLTYRQLAARARGQAAALDALGVGPGERVAIVSQNSARLLTSFF
GVSGWGRILVPINFRLAPAEIEYIVGHSGASVLVVDPEVRGLLDTVRATHNFVLGEDDAAVFGELDSPHAVTPDPWSGSE
TGTATLNYTSGTTSRPKGVQLTHRNIWINAVVFGLHATLRDDDVLLHTLPMFHCNGWGWPYAATGMGARHIVLRKVDGDE
ILRRVEQHGVTVMCAAPTVVDSILDAAARWRGPIPGRDRVRIIVAGAPPPTRTIARVRTELGWEFIQIYGLTETAPLITM
NRFRSEWAGLDVQEQARMLGRAGAPAIGVRVSVAEDGEVLTQSNTNLDGYWDQPQATAEALAGNWFHTGDRGSVDDGYLT
ISDRKKDIIITGGENVTSIEVEDALISHPGVREAAVVGVPDEKWGELVTAVVVVVTLDDPPSGADLIAHCRERLAGYKCP
KRVEFVDSLPRTATGKVQKFRLREQLARD
>Mature_509_residues
MFFDLSVRDFLDRAELVYPERVGVVDEPVQPAPSWGQLTYRQLAARARGQAAALDALGVGPGERVAIVSQNSARLLTSFF
GVSGWGRILVPINFRLAPAEIEYIVGHSGASVLVVDPEVRGLLDTVRATHNFVLGEDDAAVFGELDSPHAVTPDPWSGSE
TGTATLNYTSGTTSRPKGVQLTHRNIWINAVVFGLHATLRDDDVLLHTLPMFHCNGWGWPYAATGMGARHIVLRKVDGDE
ILRRVEQHGVTVMCAAPTVVDSILDAAARWRGPIPGRDRVRIIVAGAPPPTRTIARVRTELGWEFIQIYGLTETAPLITM
NRFRSEWAGLDVQEQARMLGRAGAPAIGVRVSVAEDGEVLTQSNTNLDGYWDQPQATAEALAGNWFHTGDRGSVDDGYLT
ISDRKKDIIITGGENVTSIEVEDALISHPGVREAAVVGVPDEKWGELVTAVVVVVTLDDPPSGADLIAHCRERLAGYKCP
KRVEFVDSLPRTATGKVQKFRLREQLARD

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=27.8095238095238, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI187761345, Length=542, Percent_Identity=28.5977859778598, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI187761343, Length=542, Percent_Identity=28.5977859778598, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI122937307, Length=547, Percent_Identity=27.6051188299817, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI42544132, Length=546, Percent_Identity=25.0915750915751, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI58082049, Length=507, Percent_Identity=26.232741617357, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI157311624, Length=506, Percent_Identity=25.8893280632411, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI157311622, Length=506, Percent_Identity=25.8893280632411, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=25.5905511811024, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI115511026, Length=141, Percent_Identity=40.4255319148936, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI28416953, Length=565, Percent_Identity=25.1327433628319, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=530, Percent_Identity=28.4905660377358, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI145693145, Length=533, Percent_Identity=28.3302063789869, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI221142682, Length=498, Percent_Identity=26.9076305220884, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1786810, Length=496, Percent_Identity=28.2258064516129, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1788595, Length=486, Percent_Identity=25.3086419753086, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790505, Length=370, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=485, Percent_Identity=31.5463917525773, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17558820, Length=480, Percent_Identity=30, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71994690, Length=520, Percent_Identity=27.5, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI71994694, Length=520, Percent_Identity=27.5, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71994703, Length=520, Percent_Identity=27.5, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=26.4875239923225, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=363, Percent_Identity=28.9256198347107, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=506, Percent_Identity=26.6798418972332, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=507, Percent_Identity=26.0355029585799, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=24.6492985971944, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=496, Percent_Identity=23.3870967741935, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=24.0458015267176, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=359, Percent_Identity=26.1838440111421, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319699, Length=492, Percent_Identity=24.5934959349593, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=133, Percent_Identity=33.0827067669173, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323182, Length=137, Percent_Identity=33.5766423357664, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581924, Length=351, Percent_Identity=30.7692307692308, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21355181, Length=487, Percent_Identity=25.8726899383984, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859661, Length=385, Percent_Identity=28.3116883116883, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922652, Length=508, Percent_Identity=27.5590551181102, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24656500, Length=512, Percent_Identity=24.4140625, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21356947, Length=371, Percent_Identity=25.8760107816712, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648257, Length=359, Percent_Identity=27.2980501392758, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130357, Length=354, Percent_Identity=25.9887005649718, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356441, Length=445, Percent_Identity=22.9213483146067, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648260, Length=361, Percent_Identity=24.0997229916898, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653035, Length=211, Percent_Identity=28.436018957346, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281365686, Length=246, Percent_Identity=26.0162601626016, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 55390; Mature: 55390

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFDLSVRDFLDRAELVYPERVGVVDEPVQPAPSWGQLTYRQLAARARGQAAALDALGVG
CEEECCHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEHHHCCC
PGERVAIVSQNSARLLTSFFGVSGWGRILVPINFRLAPAEIEYIVGHSGASVLVVDPEVR
CCCEEEEEECCCHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEECCCCEEEEECCHHH
GLLDTVRATHNFVLGEDDAAVFGELDSPHAVTPDPWSGSETGTATLNYTSGTTSRPKGVQ
HHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEE
LTHRNIWINAVVFGLHATLRDDDVLLHTLPMFHCNGWGWPYAATGMGARHIVLRKVDGDE
EEECEEEEEEEEEEEEEECCCCCEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCHHH
ILRRVEQHGVTVMCAAPTVVDSILDAAARWRGPIPGRDRVRIIVAGAPPPTRTIARVRTE
HHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
LGWEFIQIYGLTETAPLITMNRFRSEWAGLDVQEQARMLGRAGAPAIGVRVSVAEDGEVL
HCEEEEEEEECCCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEE
TQSNTNLDGYWDQPQATAEALAGNWFHTGDRGSVDDGYLTISDRKKDIIITGGENVTSIE
EECCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEEE
VEDALISHPGVREAAVVGVPDEKWGELVTAVVVVVTLDDPPSGADLIAHCRERLAGYKCP
ECHHHHCCCCCCCEEEEECCCHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCC
KRVEFVDSLPRTATGKVQKFRLREQLARD
CHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFFDLSVRDFLDRAELVYPERVGVVDEPVQPAPSWGQLTYRQLAARARGQAAALDALGVG
CEEECCHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEHHHCCC
PGERVAIVSQNSARLLTSFFGVSGWGRILVPINFRLAPAEIEYIVGHSGASVLVVDPEVR
CCCEEEEEECCCHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEECCCCEEEEECCHHH
GLLDTVRATHNFVLGEDDAAVFGELDSPHAVTPDPWSGSETGTATLNYTSGTTSRPKGVQ
HHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEE
LTHRNIWINAVVFGLHATLRDDDVLLHTLPMFHCNGWGWPYAATGMGARHIVLRKVDGDE
EEECEEEEEEEEEEEEEECCCCCEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCHHH
ILRRVEQHGVTVMCAAPTVVDSILDAAARWRGPIPGRDRVRIIVAGAPPPTRTIARVRTE
HHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
LGWEFIQIYGLTETAPLITMNRFRSEWAGLDVQEQARMLGRAGAPAIGVRVSVAEDGEVL
HCEEEEEEEECCCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEE
TQSNTNLDGYWDQPQATAEALAGNWFHTGDRGSVDDGYLTISDRKKDIIITGGENVTSIE
EECCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEEE
VEDALISHPGVREAAVVGVPDEKWGELVTAVVVVVTLDDPPSGADLIAHCRERLAGYKCP
ECHHHHCCCCCCCEEEEECCCHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCC
KRVEFVDSLPRTATGKVQKFRLREQLARD
CHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA