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The map label for this gene is narK [H]

Identifier: 169630604

GI number: 169630604

Start: 3569688

End: 3571133

Strand: Reverse

Name: narK [H]

Synonym: MAB_3523c

Alternate gene names: 169630604

Gene position: 3571133-3569688 (Counterclockwise)

Preceding gene: 169630605

Following gene: 169630603

Centisome position: 70.48

GC content: 62.52

Gene sequence:

>1446_bases
ATGGCGAACAACACGGCAACAGCCCCAATTGCACGGCGCGGACGCTGGATTGAGAACTGGGATCCCGAGGACGTCGTCGC
GTGGGACAACGGTGGCGCGAAAATCGCGCGCCGCAACCTGATCTGGTCCGTGGTGGCCGAACACGTCGGATTCTCGGTGT
GGTCCATCTGGTCGGTGATGGTGCTGTTCATGCCGCAGAACGTCTACCACATCGATGCCGCCGGGAAGTTCTTCCTGGTG
GCCATGCCGACTCTGGTGGGTGCGGTGCTGCGCTTGCCCTATACCTTCGCCACCGCATGGTTCGGCGGCCGTAACTGGAC
CGTTTTCAGCGCCCTGGTGCTGGCGGTGCCGACCGCGTTGACCCTGTATTTCATGGCTCATCCGGAAACCTCGTACACCA
CGTTCATGATCGTGGCCGCAGTCGCCGGATTCGGCGGTGGAAACTTCGCCTCCTCGATGACGAACATCAATGCCTTTTAC
CCACAACGGTTCAAGGGCTGGGCGCTGGGTCTCAATGCGGGCGGCGGGAACATCGGCGTGCCGGTCATTCAGGTGATCGG
CCTGTTGGTCATTGCCACCGTCGGGAACCGGTCGCCACACTGGGTAGCCGCCATCTACCTCGTGCTGATCGCCTTCGCGG
CGGTGGGGGCGGCGCTGTTCATGGACAACCTGGCCGACCAGAAGACCGACGGTCGCTCTCTGATCGATGTCATGAAGTAC
CGGGACTCGTGGATCATCGCCTTCCTCTACATCGGCACCTTCGGTTCCTTCATCGGATTCAGCTTCGCGTTCGGTCAGGT
GCTGCAGCTGAACTTCCTTGCCGGGCTGGCGCACAGTGGCGTGACGCCTGCGCAGGCCGCGGCGCAGGCATCGCTGCACG
CCGCTCAGATCGCGTTCATCGGCCCCTTGCTGGGCTCACTAGCCCGTCCGTTCGGTGGTTGGCTGTCGGACCGCACCGGT
GGCGGCAAGATCTCGTTGTACGCGTTCATTGCCATGATCTTCGGCGCGGGCATTCTGGTGGCCGCGGGAACGATCAGCGA
CCGCGGCGCTGGTGCGCCGTCCGGAAGCATCATGACGGCCTACGTCATCGGATTCATCATCTTGTTTGTGGTTTCCGGAA
TAGGCAATGGCTCGGTGTACAAGATGATTCCGTCGGTCTTTGCGGCTAAGGCGCGTAGTGCAGGACTCGACGAGACATGG
TCGCGCACCATGTCGGGCGCATTGATCGGGGTGGCGGGAGCGATCGGCGCCCTCGGTGGTGTCGCCATCAACCTGGTACT
TCGCGCCTCGTATCTCAGCGCGGCCAAGTCGGCGACCATGGCGTTCTGGGTGTTTCTCGTCTTCTATGTCATCTGCGCGG
CGGTCACCTGGTACGCGTACGTGCGGCGGCCCATGACCGTATCGGTTCCGGCGATCTCCAGCTCCAAAAACGAAGTGGCG
GTGTGA

Upstream 100 bases:

>100_bases
CGGATTGCTCACCGCCACCTGACAGACTGAGTGAGCGAGATGTGATGGCTCGGTCATCGGCAGCAGCGCCCCTGCAACAA
GCTTTTCCTACCGTGCTTGT

Downstream 100 bases:

>100_bases
AACCCATGAACAAGAAAGTCGTCGTCATCGGCCATGGAATGGTCGGTCACCGATTTGTCCAGGTGCTGCGTGAGCGCGAT
GCCACCGATCAGTGGCAGGT

Product: integral membrane nitrite extrusion protein NarK3

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MANNTATAPIARRGRWIENWDPEDVVAWDNGGAKIARRNLIWSVVAEHVGFSVWSIWSVMVLFMPQNVYHIDAAGKFFLV
AMPTLVGAVLRLPYTFATAWFGGRNWTVFSALVLAVPTALTLYFMAHPETSYTTFMIVAAVAGFGGGNFASSMTNINAFY
PQRFKGWALGLNAGGGNIGVPVIQVIGLLVIATVGNRSPHWVAAIYLVLIAFAAVGAALFMDNLADQKTDGRSLIDVMKY
RDSWIIAFLYIGTFGSFIGFSFAFGQVLQLNFLAGLAHSGVTPAQAAAQASLHAAQIAFIGPLLGSLARPFGGWLSDRTG
GGKISLYAFIAMIFGAGILVAAGTISDRGAGAPSGSIMTAYVIGFIILFVVSGIGNGSVYKMIPSVFAAKARSAGLDETW
SRTMSGALIGVAGAIGALGGVAINLVLRASYLSAAKSATMAFWVFLVFYVICAAVTWYAYVRRPMTVSVPAISSSKNEVA
V

Sequences:

>Translated_481_residues
MANNTATAPIARRGRWIENWDPEDVVAWDNGGAKIARRNLIWSVVAEHVGFSVWSIWSVMVLFMPQNVYHIDAAGKFFLV
AMPTLVGAVLRLPYTFATAWFGGRNWTVFSALVLAVPTALTLYFMAHPETSYTTFMIVAAVAGFGGGNFASSMTNINAFY
PQRFKGWALGLNAGGGNIGVPVIQVIGLLVIATVGNRSPHWVAAIYLVLIAFAAVGAALFMDNLADQKTDGRSLIDVMKY
RDSWIIAFLYIGTFGSFIGFSFAFGQVLQLNFLAGLAHSGVTPAQAAAQASLHAAQIAFIGPLLGSLARPFGGWLSDRTG
GGKISLYAFIAMIFGAGILVAAGTISDRGAGAPSGSIMTAYVIGFIILFVVSGIGNGSVYKMIPSVFAAKARSAGLDETW
SRTMSGALIGVAGAIGALGGVAINLVLRASYLSAAKSATMAFWVFLVFYVICAAVTWYAYVRRPMTVSVPAISSSKNEVA
V
>Mature_480_residues
ANNTATAPIARRGRWIENWDPEDVVAWDNGGAKIARRNLIWSVVAEHVGFSVWSIWSVMVLFMPQNVYHIDAAGKFFLVA
MPTLVGAVLRLPYTFATAWFGGRNWTVFSALVLAVPTALTLYFMAHPETSYTTFMIVAAVAGFGGGNFASSMTNINAFYP
QRFKGWALGLNAGGGNIGVPVIQVIGLLVIATVGNRSPHWVAAIYLVLIAFAAVGAALFMDNLADQKTDGRSLIDVMKYR
DSWIIAFLYIGTFGSFIGFSFAFGQVLQLNFLAGLAHSGVTPAQAAAQASLHAAQIAFIGPLLGSLARPFGGWLSDRTGG
GKISLYAFIAMIFGAGILVAAGTISDRGAGAPSGSIMTAYVIGFIILFVVSGIGNGSVYKMIPSVFAAKARSAGLDETWS
RTMSGALIGVAGAIGALGGVAINLVLRASYLSAAKSATMAFWVFLVFYVICAAVTWYAYVRRPMTVSVPAISSSKNEVAV

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=482, Percent_Identity=35.2697095435685, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1787743, Length=474, Percent_Identity=33.9662447257384, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50900; Mature: 50769

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNTATAPIARRGRWIENWDPEDVVAWDNGGAKIARRNLIWSVVAEHVGFSVWSIWSVM
CCCCCCCCCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
VLFMPQNVYHIDAAGKFFLVAMPTLVGAVLRLPYTFATAWFGGRNWTVFSALVLAVPTAL
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLYFMAHPETSYTTFMIVAAVAGFGGGNFASSMTNINAFYPQRFKGWALGLNAGGGNIGV
HHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCH
PVIQVIGLLVIATVGNRSPHWVAAIYLVLIAFAAVGAALFMDNLADQKTDGRSLIDVMKY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RDSWIIAFLYIGTFGSFIGFSFAFGQVLQLNFLAGLAHSGVTPAQAAAQASLHAAQIAFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GPLLGSLARPFGGWLSDRTGGGKISLYAFIAMIFGAGILVAAGTISDRGAGAPSGSIMTA
HHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCHHHH
YVIGFIILFVVSGIGNGSVYKMIPSVFAAKARSAGLDETWSRTMSGALIGVAGAIGALGG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VAINLVLRASYLSAAKSATMAFWVFLVFYVICAAVTWYAYVRRPMTVSVPAISSSKNEVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
V
C
>Mature Secondary Structure 
ANNTATAPIARRGRWIENWDPEDVVAWDNGGAKIARRNLIWSVVAEHVGFSVWSIWSVM
CCCCCCCCHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]