Click here to switch to the map view.

The map label for this gene is alsS [H]

Identifier: 169630551

GI number: 169630551

Start: 3510305

End: 3511894

Strand: Reverse

Name: alsS [H]

Synonym: MAB_3470c

Alternate gene names: 169630551

Gene position: 3511894-3510305 (Counterclockwise)

Preceding gene: 169630554

Following gene: 169630548

Centisome position: 69.31

GC content: 62.96

Gene sequence:

>1590_bases
GTGGTCTTCGGAATACCGGGGGAAGAGAACATCCGGTTTATCCAGGCGCTCGCGGCATCGGACATCCGCTACATTCTGAC
CCGTCACGAGCAGGGCGCGGCGTTCATGGCGGAAATGTATGGCCGGGTCACCGGGCGTGCCGGGGTGGTGTCCGCCACGT
TGGGTCCTGGGGCGATCAACCTACAGCTGGGAGTCGCCGACGCGTCGACGAACAGCACACCTTTGGTCGCGATCGCCGCC
CAGGTCGGCCAGGATCGGGCTTACAAAGAATCGCATCAATACGTGGACCTGGTGGCCATGTTCCGTCCGATCACCCGGTG
GGCGGATGGCGTTCCTACCGCCCGGGCCATCCCGGAGATGTTCCGCAAGGCCTTCAAGCTTGCCGAGACCGAACGGCCGG
CGGCCGTATTTCTGGCAGTGCCCGAGAACATCGACGCAGACGGTGCGAACTATGAGCTGAACCCATTGCCGCGTAATGTG
GTACACCCCGAAGCGCCGATGGCCGGTCAAGTACAGCGCGCGGTTGACATCCTGCGGAGGGCGCATCGGCCGGTGCTACT
TGCCGGTCACGGCGCCGCTCGTGGAGGTGCGACGGCGGCATTGATTCGGTTCTCCGAGGAGCTCGGTGTGCCGGTGGCCA
ACACCTTCCACGGTAAGGGTGCGATGCCCGATGACCATCCGAACAGCATGGGGACGGTCGGCTTTATGCGGCGTGACTAC
GCCAATTTCGGCTTTGAGCACGCCGACGTGATTGTTGCGGTGGGGTATGAGCTGCAAGAGTTCGACCCGGTGCGGATCAA
CCCGCATGGCGACAAGCAGATCATCCATCTCCATAGGTTCCCGGCCGAAGTCGACGCCCACTATTCGGTGTCGGTGGGAA
TCATCGGTGATATCAGCGCTTCGGTGAACGCGCTCACCGATGCCCTCGCTGGGCATCGTTTCGACTGCGCCGCAGCTCCG
GGATCCGCTCTGCTCGCCGAGGAATTTACTCGGGGACAACAGGATTCACGTTTCCCCATGGCGCCGCAGCGGATCGTGGC
CGACATCCGGGCAGCGCTGGGCCGCAGCGACGTCGTGCTTGTCGATACCGGTGCCACCAAGATGTGGATGGCGCGTCTTT
ACCCGACCTATGAGCCCAATACCTGCCTTGTCTCTAATGGGCTGTCCACCATGGGTTTCGCACTGCCTGGAGCGCTGGGC
GTCAAGTTGGCCCGCCCCGATACGAAGGTGTTGGCGGTCGCCGGCGATGGCGCCTTCCTGATGAACTCACAGGAGATCGA
GACTGCCGTACGAGAGCGCATCCCGCTGGTGGTTTTGATCTGGGAGGACGGCGGCTACGGGCTCATCGAGTGGAAGATGG
ACCTCGAACTCGGGGAACACCACCACGTGTCGTTCACCAATCCCGACATCGTGTCCTACGCCGAGAGCTTCGGAGCCAAG
GGCTATCGCATCACCGCGGCCGACCAATTGCTGCCGACGCTGCAAGCGGCGCTTGACGATGGCGGTGTCTCGCTGATCGC
CTGCCCGGTCGACTACTCGGAGAATCTGAGGCTCACCGATCGGCTGGGGCAACTGGACGCGACTTTGTAG

Upstream 100 bases:

>100_bases
ATCGTTGCGATAGGCATGTCTCCAGCGTGCCACTACCGTAGGAAAGATGAGCACAGCTGCCCAATTAATGGTGAAGTGTT
TGGAGAACGAGGGCGTTTCT

Downstream 100 bases:

>100_bases
CAGCTGCTTGCGATCACCCGAATCCCGTGCGGCTATGGCCGCGGCCGGTTTCCAGACAGGATGGGAAGCACGTGTTCGGC
CAGCAGCGGCGCCAGGTCTG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MVFGIPGEENIRFIQALAASDIRYILTRHEQGAAFMAEMYGRVTGRAGVVSATLGPGAINLQLGVADASTNSTPLVAIAA
QVGQDRAYKESHQYVDLVAMFRPITRWADGVPTARAIPEMFRKAFKLAETERPAAVFLAVPENIDADGANYELNPLPRNV
VHPEAPMAGQVQRAVDILRRAHRPVLLAGHGAARGGATAALIRFSEELGVPVANTFHGKGAMPDDHPNSMGTVGFMRRDY
ANFGFEHADVIVAVGYELQEFDPVRINPHGDKQIIHLHRFPAEVDAHYSVSVGIIGDISASVNALTDALAGHRFDCAAAP
GSALLAEEFTRGQQDSRFPMAPQRIVADIRAALGRSDVVLVDTGATKMWMARLYPTYEPNTCLVSNGLSTMGFALPGALG
VKLARPDTKVLAVAGDGAFLMNSQEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGEHHHVSFTNPDIVSYAESFGAK
GYRITAADQLLPTLQAALDDGGVSLIACPVDYSENLRLTDRLGQLDATL

Sequences:

>Translated_529_residues
MVFGIPGEENIRFIQALAASDIRYILTRHEQGAAFMAEMYGRVTGRAGVVSATLGPGAINLQLGVADASTNSTPLVAIAA
QVGQDRAYKESHQYVDLVAMFRPITRWADGVPTARAIPEMFRKAFKLAETERPAAVFLAVPENIDADGANYELNPLPRNV
VHPEAPMAGQVQRAVDILRRAHRPVLLAGHGAARGGATAALIRFSEELGVPVANTFHGKGAMPDDHPNSMGTVGFMRRDY
ANFGFEHADVIVAVGYELQEFDPVRINPHGDKQIIHLHRFPAEVDAHYSVSVGIIGDISASVNALTDALAGHRFDCAAAP
GSALLAEEFTRGQQDSRFPMAPQRIVADIRAALGRSDVVLVDTGATKMWMARLYPTYEPNTCLVSNGLSTMGFALPGALG
VKLARPDTKVLAVAGDGAFLMNSQEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGEHHHVSFTNPDIVSYAESFGAK
GYRITAADQLLPTLQAALDDGGVSLIACPVDYSENLRLTDRLGQLDATL
>Mature_529_residues
MVFGIPGEENIRFIQALAASDIRYILTRHEQGAAFMAEMYGRVTGRAGVVSATLGPGAINLQLGVADASTNSTPLVAIAA
QVGQDRAYKESHQYVDLVAMFRPITRWADGVPTARAIPEMFRKAFKLAETERPAAVFLAVPENIDADGANYELNPLPRNV
VHPEAPMAGQVQRAVDILRRAHRPVLLAGHGAARGGATAALIRFSEELGVPVANTFHGKGAMPDDHPNSMGTVGFMRRDY
ANFGFEHADVIVAVGYELQEFDPVRINPHGDKQIIHLHRFPAEVDAHYSVSVGIIGDISASVNALTDALAGHRFDCAAAP
GSALLAEEFTRGQQDSRFPMAPQRIVADIRAALGRSDVVLVDTGATKMWMARLYPTYEPNTCLVSNGLSTMGFALPGALG
VKLARPDTKVLAVAGDGAFLMNSQEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGEHHHVSFTNPDIVSYAESFGAK
GYRITAADQLLPTLQAALDDGGVSLIACPVDYSENLRLTDRLGQLDATL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=533, Percent_Identity=27.0168855534709, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI21361361, Length=549, Percent_Identity=26.4116575591985, Blast_Score=139, Evalue=9e-33,
Organism=Escherichia coli, GI87081685, Length=545, Percent_Identity=30.6422018348624, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1790104, Length=531, Percent_Identity=28.6252354048964, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1786717, Length=521, Percent_Identity=28.0230326295585, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787096, Length=496, Percent_Identity=24.7983870967742, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788716, Length=542, Percent_Identity=26.5682656826568, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=471, Percent_Identity=27.8131634819533, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=472, Percent_Identity=28.1779661016949, Blast_Score=138, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=540, Percent_Identity=26.6666666666667, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6320816, Length=477, Percent_Identity=27.2536687631027, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323163, Length=511, Percent_Identity=22.3091976516634, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=519, Percent_Identity=22.9287090558767, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19922626, Length=524, Percent_Identity=28.2442748091603, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56996; Mature: 56996

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00141 ASP_PROTEASE ; PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFGIPGEENIRFIQALAASDIRYILTRHEQGAAFMAEMYGRVTGRAGVVSATLGPGAIN
CEECCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCEEE
LQLGVADASTNSTPLVAIAAQVGQDRAYKESHQYVDLVAMFRPITRWADGVPTARAIPEM
EEEEEEECCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FRKAFKLAETERPAAVFLAVPENIDADGANYELNPLPRNVVHPEAPMAGQVQRAVDILRR
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
AHRPVLLAGHGAARGGATAALIRFSEELGVPVANTFHGKGAMPDDHPNSMGTVGFMRRDY
CCCCEEEECCCCCCCCCEEEEEEEHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHH
ANFGFEHADVIVAVGYELQEFDPVRINPHGDKQIIHLHRFPAEVDAHYSVSVGIIGDISA
HHCCCCCCCEEEEECCCHHCCCCEEECCCCCCEEEEEEECCCCCCCCEEEEEEEEECCCH
SVNALTDALAGHRFDCAAAPGSALLAEEFTRGQQDSRFPMAPQRIVADIRAALGRSDVVL
HHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
VDTGATKMWMARLYPTYEPNTCLVSNGLSTMGFALPGALGVKLARPDTKVLAVAGDGAFL
EECCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCHHCEEEECCCCEEEEEECCCEEE
MNSQEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGEHHHVSFTNPDIVSYAESFGAK
ECCHHHHHHHHHCCCEEEEEEECCCEEEEEEEEEEECCCCEEEECCCCHHHHHHHHHCCC
GYRITAADQLLPTLQAALDDGGVSLIACPVDYSENLRLTDRLGQLDATL
CEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MVFGIPGEENIRFIQALAASDIRYILTRHEQGAAFMAEMYGRVTGRAGVVSATLGPGAIN
CEECCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCEEE
LQLGVADASTNSTPLVAIAAQVGQDRAYKESHQYVDLVAMFRPITRWADGVPTARAIPEM
EEEEEEECCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FRKAFKLAETERPAAVFLAVPENIDADGANYELNPLPRNVVHPEAPMAGQVQRAVDILRR
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
AHRPVLLAGHGAARGGATAALIRFSEELGVPVANTFHGKGAMPDDHPNSMGTVGFMRRDY
CCCCEEEECCCCCCCCCEEEEEEEHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHH
ANFGFEHADVIVAVGYELQEFDPVRINPHGDKQIIHLHRFPAEVDAHYSVSVGIIGDISA
HHCCCCCCCEEEEECCCHHCCCCEEECCCCCCEEEEEEECCCCCCCCEEEEEEEEECCCH
SVNALTDALAGHRFDCAAAPGSALLAEEFTRGQQDSRFPMAPQRIVADIRAALGRSDVVL
HHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
VDTGATKMWMARLYPTYEPNTCLVSNGLSTMGFALPGALGVKLARPDTKVLAVAGDGAFL
EECCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCHHCEEEECCCCEEEEEECCCEEE
MNSQEIETAVRERIPLVVLIWEDGGYGLIEWKMDLELGEHHHVSFTNPDIVSYAESFGAK
ECCHHHHHHHHHCCCEEEEEEECCCEEEEEEEEEEECCCCEEEECCCCHHHHHHHHHCCC
GYRITAADQLLPTLQAALDDGGVSLIACPVDYSENLRLTDRLGQLDATL
CEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]