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The map label for this gene is hdhA [C]

Identifier: 169630521

GI number: 169630521

Start: 3484060

End: 3484983

Strand: Reverse

Name: hdhA [C]

Synonym: MAB_3440c

Alternate gene names: 169630521

Gene position: 3484983-3484060 (Counterclockwise)

Preceding gene: 169630522

Following gene: 169630518

Centisome position: 68.78

GC content: 64.94

Gene sequence:

>924_bases
GTGTTTGGAACTGACGTCCGGTTCGACGGAAGAGTGGCCATTGTCACCGGAGCGGGCGGTGGGCTCGGCCGCGAGTACGC
TCTGCTGTTGGCAAGCCGTGGCGCCAAAGTGGTGGTCAACGACATCGGATCGTCCGACGAGCTGCTGGGCGGTGGTAGCT
CCGATGCACCCGCAAACTCGGTGGTTGCCGAGATTCAGGCCCTCGGCGGCGACGCCATCGCAGACACGAACACCGTCGCT
ACCGGGGCAGGTGGACGCGCCATCGTCGGCACCGCGCTGTCGGCCTGGGGGAGAGTCGACATCGTCATCAACAATGCGGG
AATCGTGGGGCCGATCAAGACTTTCGCCGAACTCACCGACGAGGACGTGGACACCGTGTTCGGAGTTCAGTTACTCGGGC
CGTTCAACGTGCTGCGCCCGGCGTGGAAGGCCATGGTCGATCAGGGCTACGGACGGATCCTGAACATCTCATCCGGGAGC
ATATTCGGGCAGGGGGAGGCCTGCACCTATCCAACCGCGAAGGCCGGAATGGTCGGCATGACCACCAATTTGGGGCTGGC
CGGCCCGGCATATGGCATCAAGGTGAATGCGCTACTGCCGGTGGGGTTCACCCGGATGGTGGAGAACATGCCGGAGCCGG
CGCTGAGCTGGCTCCGCGACACCTTCCCGGCCGCGTTGGTGGCGCCCGTTGCCGCATACCTGGTATCCGAAGATGTGCCG
TGCTCGGGTCTCAGTTTCAGCGCCGGCGGTGGACGATTCGCGCGAGTCTTCCTCGGTGAGGCACGGGGAGTGACTTCCTC
GCAGCTCACCATCGAGGAGGTTCGTGACAGGTTCGAGGCGGCGATGGAAATCGAAGGCGCCGCCACCATGGCCAATGTTG
GCGACGAGCTCAGGCTCTACGCGGCGGCGCTGACCGGCCGGTGA

Upstream 100 bases:

>100_bases
GGCTCGTGACCATCCTTGCGCCTGATCCGTTATTTGCGATAACGTATCTGCCGAGGTGGGATTCAACTCGACGTCGAGAT
GAATTTGCTTGGAGGCGTGT

Downstream 100 bases:

>100_bases
TACCTCACGCCTGATAAGTGGGCAGCACCTTTTCCCAGTCTTCGATGTTGCCGCGAACCGGCCGAAAGATGGGATGCGGG
CGCAGTCGGCGGGGATTGCT

Product: putative short-chain dehydrogenase/reductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MFGTDVRFDGRVAIVTGAGGGLGREYALLLASRGAKVVVNDIGSSDELLGGGSSDAPANSVVAEIQALGGDAIADTNTVA
TGAGGRAIVGTALSAWGRVDIVINNAGIVGPIKTFAELTDEDVDTVFGVQLLGPFNVLRPAWKAMVDQGYGRILNISSGS
IFGQGEACTYPTAKAGMVGMTTNLGLAGPAYGIKVNALLPVGFTRMVENMPEPALSWLRDTFPAALVAPVAAYLVSEDVP
CSGLSFSAGGGRFARVFLGEARGVTSSQLTIEEVRDRFEAAMEIEGAATMANVGDELRLYAAALTGR

Sequences:

>Translated_307_residues
MFGTDVRFDGRVAIVTGAGGGLGREYALLLASRGAKVVVNDIGSSDELLGGGSSDAPANSVVAEIQALGGDAIADTNTVA
TGAGGRAIVGTALSAWGRVDIVINNAGIVGPIKTFAELTDEDVDTVFGVQLLGPFNVLRPAWKAMVDQGYGRILNISSGS
IFGQGEACTYPTAKAGMVGMTTNLGLAGPAYGIKVNALLPVGFTRMVENMPEPALSWLRDTFPAALVAPVAAYLVSEDVP
CSGLSFSAGGGRFARVFLGEARGVTSSQLTIEEVRDRFEAAMEIEGAATMANVGDELRLYAAALTGR
>Mature_307_residues
MFGTDVRFDGRVAIVTGAGGGLGREYALLLASRGAKVVVNDIGSSDELLGGGSSDAPANSVVAEIQALGGDAIADTNTVA
TGAGGRAIVGTALSAWGRVDIVINNAGIVGPIKTFAELTDEDVDTVFGVQLLGPFNVLRPAWKAMVDQGYGRILNISSGS
IFGQGEACTYPTAKAGMVGMTTNLGLAGPAYGIKVNALLPVGFTRMVENMPEPALSWLRDTFPAALVAPVAAYLVSEDVP
CSGLSFSAGGGRFARVFLGEARGVTSSQLTIEEVRDRFEAAMEIEGAATMANVGDELRLYAAALTGR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=300, Percent_Identity=38, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI15277342, Length=266, Percent_Identity=31.203007518797, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4758504, Length=244, Percent_Identity=31.5573770491803, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI83715985, Length=245, Percent_Identity=30.6122448979592, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=29.0196078431373, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787905, Length=159, Percent_Identity=38.9937106918239, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787335, Length=260, Percent_Identity=28.0769230769231, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789208, Length=264, Percent_Identity=26.8939393939394, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082160, Length=196, Percent_Identity=30.6122448979592, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788459, Length=192, Percent_Identity=30.2083333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789057, Length=200, Percent_Identity=32.5, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=293, Percent_Identity=37.542662116041, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI193204405, Length=247, Percent_Identity=28.7449392712551, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=205, Percent_Identity=32.6829268292683, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=256, Percent_Identity=28.90625, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=204, Percent_Identity=30.8823529411765, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=224, Percent_Identity=29.0178571428571, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=222, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=234, Percent_Identity=32.4786324786325, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=209, Percent_Identity=27.7511961722488, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=207, Percent_Identity=27.536231884058, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=211, Percent_Identity=29.8578199052133, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=213, Percent_Identity=26.2910798122066, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=205, Percent_Identity=26.8292682926829, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=240, Percent_Identity=28.75, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=227, Percent_Identity=29.5154185022026, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=264, Percent_Identity=39.7727272727273, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI18859837, Length=297, Percent_Identity=36.7003367003367, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17737361, Length=225, Percent_Identity=30.6666666666667, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639444, Length=222, Percent_Identity=31.5315315315315, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 31480; Mature: 31480

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTDVRFDGRVAIVTGAGGGLGREYALLLASRGAKVVVNDIGSSDELLGGGSSDAPANS
CCCCCEEECCEEEEEEECCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHH
VVAEIQALGGDAIADTNTVATGAGGRAIVGTALSAWGRVDIVINNAGIVGPIKTFAELTD
HHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCC
EDVDTVFGVQLLGPFNVLRPAWKAMVDQGYGRILNISSGSIFGQGEACTYPTAKAGMVGM
CHHHHHHHHHEECCHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCEEEE
TTNLGLAGPAYGIKVNALLPVGFTRMVENMPEPALSWLRDTFPAALVAPVAAYLVSEDVP
ECCCCCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
CSGLSFSAGGGRFARVFLGEARGVTSSQLTIEEVRDRFEAAMEIEGAATMANVGDELRLY
CCCCEECCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHCCHHHEEE
AAALTGR
EEECCCC
>Mature Secondary Structure
MFGTDVRFDGRVAIVTGAGGGLGREYALLLASRGAKVVVNDIGSSDELLGGGSSDAPANS
CCCCCEEECCEEEEEEECCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHH
VVAEIQALGGDAIADTNTVATGAGGRAIVGTALSAWGRVDIVINNAGIVGPIKTFAELTD
HHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCC
EDVDTVFGVQLLGPFNVLRPAWKAMVDQGYGRILNISSGSIFGQGEACTYPTAKAGMVGM
CHHHHHHHHHEECCHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCEEEE
TTNLGLAGPAYGIKVNALLPVGFTRMVENMPEPALSWLRDTFPAALVAPVAAYLVSEDVP
ECCCCCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
CSGLSFSAGGGRFARVFLGEARGVTSSQLTIEEVRDRFEAAMEIEGAATMANVGDELRLY
CCCCEECCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHCCHHHEEE
AAALTGR
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]