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The map label for this gene is nrdE [H]

Identifier: 169630494

GI number: 169630494

Start: 3459297

End: 3461435

Strand: Reverse

Name: nrdE [H]

Synonym: MAB_3413c

Alternate gene names: 169630494

Gene position: 3461435-3459297 (Counterclockwise)

Preceding gene: 169630495

Following gene: 169630490

Centisome position: 68.31

GC content: 59.23

Gene sequence:

>2139_bases
GTGACCGCTGCGCACGGCTCGGCTCACGGCGAGCTGGACTATCACGCACTCAACGCGATGCTCAATCTGTATGACGCAGA
GGGCAAGATCCAGTTCGACAAGGACGCGGCGGCGGCGCGGCAGTACTTTCTGCAGCACGTCAACCAGAACACGGTCTTCT
TCCACAATCAGGACGAGAAGCTCGATTACCTGATCGAGAAGGAGTACTACGAGCGTGAGGTGCTCGACCAGTACTCGCGC
AACTTCGTGAAGTCGCTGCTGGACCGGGCGTACGCCAAGAAGTTCCGATTCCCGACCTTCCTGGGCGCGTTCAAGTACTA
CACCTCATACACGCTCAAGACGTTCGACGGGAAGCGCTACCTGGAGCGCTTCGAGGATCGCGTCGTCATGGTGGCGCTGA
CCCTGGCCGCCGGCGATCTGCAGCTGGCCGAGAAGCTGGTCGACGAGATCATCGACGGACGCTTCCAGCCGGCCACCCCG
ACCTTCCTCAACTCGGGTAAGAAGCAGCGCGGCGAGCCGGTGTCCTGCTTCCTGCTGCGCATCGAGGACAACATGGAGTC
CATCGGCCGCTCCATCAACTCGGCACTGCAGCTGTCCAAGCGCGGTGGCGGTGTTGCCTTGCTGCTCACCAATATTCGCG
AGCACGGGGCGCCGATCAAGAACATCGAGAACCAGAGTTCGGGTGTCATCCCGATCATGAAGCTGTTGGAGGACTCCTTC
TCCTATGCCAATCAGCTCGGTGCCCGCCAGGGTGCCGGTGCGGTCTACCTGCATGCGCACCACCCGGACATCTACCGGTT
CTTGGACACCAAGCGTGAGAACGCCGATGAAAAGATTCGGATCAAGACACTCTCGTTGGGCGTGGTGATTCCGGACATCA
CCTTTGAGCTGGCCAAGAAGAACGAGGACATGTACTTGTTCTCGCCGTATGACGTCGAGCGTACCTATGGGGTGCCGTTC
GCCGACATCAACGTCACCGAAAAGTATTACGAGATGGTCGATAACGGCCAGATCCGCAAGACGAAGATCAAGGCGCGTGA
GTTCTTCCAGACCCTGGCCGAGCTGCAGTTCGAGTCGGGCTACCCGTACATCATGTACGAGGACACCGTGAACCGGGCCA
ACCCCATCGAGGGCAAGATCACTCACTCGAACCTGTGCTCGGAGATCCTGCAGGTATCGACGCCCTCGCTGTTCAACGAC
GATCTGTCCTACGCCAAGGTGGGCAAGGATATTTCGTGCAATCTGGGTTCGCTCAACATTGCCAAGACGATGGATTCGCC
GGACTTCGCGCAGACCATCGAGGTGGCGATCCGTGCACTGACCGCGGTGTCCGATCAGACCCACATCTGGTCGGTGCCGT
CAATCGAGCAGGGCAACAACGACTCTCATGCCATCGGTCTGGGGCAGATGAACCTGCACGGCTATTTGGCGCGCGAGCGG
ATTTTCTACGGTTCGGAAGAGGGTGTGGATTTCACCAACATCTACTTCTACACCGTGCTTTACCACGCGCTGAGGGCGTC
GAACCGCATTGCCATTGAGCGCGGTACGAAGTTCGGCGGGTTCGACAGGTCGAAGTACGCCAGCGGCGAGTTCTTCGACA
AATACACCGACCAGGTGTGGGAGCCGAAGACCGAGAAGGTGCGTCAGCTGTTTGCCGATGCCGGGATTCGCATTCCGACA
CAGGATGATTGGCTGCGGTTGAAGGAATCGGTGCAGCAGCACGGCATCTACAACCAGAATCTGCAGGCGGTGCCGCCGAC
GGGATCGATCTCCTACATCAACCACTCGACGTCGTCGATTCACCCGGTTGCCTCGAAGATCGAGATCCGTAAGGAAGGCA
AGATCGGCCGGGCCTACTATCCGGCGCCGTACCTGACGAACGACAACCTGGAGTACTACCAGGACGCGTACGAGATCGGC
TACGAGAAGATCATCGACACCTACGCCGCGGCCACTCAGCACGTGGATCAGGGTTTGTCGCTGACGCTGTTCTTCAAGGA
CACCGCCAGCACGCGCGACGTGAACAAGGCGCAGATTTACGCTTGGCGCAAGGGAATCAAGACGCTGTACTACATCCGGT
TGCGCCAGATGGCGCTGGAGGGCACCGAGGTCGAGGGCTGCGTGTCCTGCATGCTGTAG

Upstream 100 bases:

>100_bases
AACTCATGGGCACTGCTGAAGACGTAGAGCAAGTGCTCGACGGACTCGATGAATTTTGGAAGGACACACCGTGGCGCCAA
CCGCGACAACTGCAGAACCA

Downstream 100 bases:

>100_bases
AGGTTTTGGATAACAAGCGGGCCAGGAGGATTCTCCTGGCCCGCTTGCATGTCGAAGGTCACTCGCGACGGCGTGATCGT
CCTACTTGCGGCGCTGTTTC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 712; Mature: 711

Protein sequence:

>712_residues
MTAAHGSAHGELDYHALNAMLNLYDAEGKIQFDKDAAAARQYFLQHVNQNTVFFHNQDEKLDYLIEKEYYEREVLDQYSR
NFVKSLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDLQLAEKLVDEIIDGRFQPATP
TFLNSGKKQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDSF
SYANQLGARQGAGAVYLHAHHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKKNEDMYLFSPYDVERTYGVPF
ADINVTEKYYEMVDNGQIRKTKIKAREFFQTLAELQFESGYPYIMYEDTVNRANPIEGKITHSNLCSEILQVSTPSLFND
DLSYAKVGKDISCNLGSLNIAKTMDSPDFAQTIEVAIRALTAVSDQTHIWSVPSIEQGNNDSHAIGLGQMNLHGYLARER
IFYGSEEGVDFTNIYFYTVLYHALRASNRIAIERGTKFGGFDRSKYASGEFFDKYTDQVWEPKTEKVRQLFADAGIRIPT
QDDWLRLKESVQQHGIYNQNLQAVPPTGSISYINHSTSSIHPVASKIEIRKEGKIGRAYYPAPYLTNDNLEYYQDAYEIG
YEKIIDTYAAATQHVDQGLSLTLFFKDTASTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML

Sequences:

>Translated_712_residues
MTAAHGSAHGELDYHALNAMLNLYDAEGKIQFDKDAAAARQYFLQHVNQNTVFFHNQDEKLDYLIEKEYYEREVLDQYSR
NFVKSLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDLQLAEKLVDEIIDGRFQPATP
TFLNSGKKQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDSF
SYANQLGARQGAGAVYLHAHHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKKNEDMYLFSPYDVERTYGVPF
ADINVTEKYYEMVDNGQIRKTKIKAREFFQTLAELQFESGYPYIMYEDTVNRANPIEGKITHSNLCSEILQVSTPSLFND
DLSYAKVGKDISCNLGSLNIAKTMDSPDFAQTIEVAIRALTAVSDQTHIWSVPSIEQGNNDSHAIGLGQMNLHGYLARER
IFYGSEEGVDFTNIYFYTVLYHALRASNRIAIERGTKFGGFDRSKYASGEFFDKYTDQVWEPKTEKVRQLFADAGIRIPT
QDDWLRLKESVQQHGIYNQNLQAVPPTGSISYINHSTSSIHPVASKIEIRKEGKIGRAYYPAPYLTNDNLEYYQDAYEIG
YEKIIDTYAAATQHVDQGLSLTLFFKDTASTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML
>Mature_711_residues
TAAHGSAHGELDYHALNAMLNLYDAEGKIQFDKDAAAARQYFLQHVNQNTVFFHNQDEKLDYLIEKEYYEREVLDQYSRN
FVKSLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDLQLAEKLVDEIIDGRFQPATPT
FLNSGKKQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDSFS
YANQLGARQGAGAVYLHAHHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKKNEDMYLFSPYDVERTYGVPFA
DINVTEKYYEMVDNGQIRKTKIKAREFFQTLAELQFESGYPYIMYEDTVNRANPIEGKITHSNLCSEILQVSTPSLFNDD
LSYAKVGKDISCNLGSLNIAKTMDSPDFAQTIEVAIRALTAVSDQTHIWSVPSIEQGNNDSHAIGLGQMNLHGYLARERI
FYGSEEGVDFTNIYFYTVLYHALRASNRIAIERGTKFGGFDRSKYASGEFFDKYTDQVWEPKTEKVRQLFADAGIRIPTQ
DDWLRLKESVQQHGIYNQNLQAVPPTGSISYINHSTSSIHPVASKIEIRKEGKIGRAYYPAPYLTNDNLEYYQDAYEIGY
EKIIDTYAAATQHVDQGLSLTLFFKDTASTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=682, Percent_Identity=26.099706744868, Blast_Score=194, Evalue=3e-49,
Organism=Escherichia coli, GI1789030, Length=711, Percent_Identity=71.4486638537271, Blast_Score=1081, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=578, Percent_Identity=26.8166089965398, Blast_Score=180, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17555242, Length=642, Percent_Identity=26.9470404984424, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6322123, Length=654, Percent_Identity=26.7584097859327, Blast_Score=186, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6320914, Length=655, Percent_Identity=26.5648854961832, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17352481, Length=639, Percent_Identity=25.1956181533646, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81080; Mature: 80949

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAHGSAHGELDYHALNAMLNLYDAEGKIQFDKDAAAARQYFLQHVNQNTVFFHNQDEK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEECCCCH
LDYLIEKEYYEREVLDQYSRNFVKSLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCHHH
LERFEDRVVMVALTLAAGDLQLAEKLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLLR
HHHHHCCEEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHCCCCEEEEEEE
IEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDSF
ECCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
SYANQLGARQGAGAVYLHAHHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKK
HHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHCC
NEDMYLFSPYDVERTYGVPFADINVTEKYYEMVDNGQIRKTKIKAREFFQTLAELQFESG
CCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCC
YPYIMYEDTVNRANPIEGKITHSNLCSEILQVSTPSLFNDDLSYAKVGKDISCNLGSLNI
CCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCEEE
AKTMDSPDFAQTIEVAIRALTAVSDQTHIWSVPSIEQGNNDSHAIGLGQMNLHGYLARER
EECCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHH
IFYGSEEGVDFTNIYFYTVLYHALRASNRIAIERGTKFGGFDRSKYASGEFFDKYTDQVW
EEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHC
EPKTEKVRQLFADAGIRIPTQDDWLRLKESVQQHGIYNQNLQAVPPTGSISYINHSTSSI
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCC
HPVASKIEIRKEGKIGRAYYPAPYLTNDNLEYYQDAYEIGYEKIIDTYAAATQHVDQGLS
CHHHHHEEEECCCCCCCEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LTLFFKDTASTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TAAHGSAHGELDYHALNAMLNLYDAEGKIQFDKDAAAARQYFLQHVNQNTVFFHNQDEK
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEECCCCH
LDYLIEKEYYEREVLDQYSRNFVKSLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCHHH
LERFEDRVVMVALTLAAGDLQLAEKLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLLR
HHHHHCCEEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHCCCCEEEEEEE
IEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDSF
ECCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
SYANQLGARQGAGAVYLHAHHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKK
HHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHCC
NEDMYLFSPYDVERTYGVPFADINVTEKYYEMVDNGQIRKTKIKAREFFQTLAELQFESG
CCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCC
YPYIMYEDTVNRANPIEGKITHSNLCSEILQVSTPSLFNDDLSYAKVGKDISCNLGSLNI
CCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCEEE
AKTMDSPDFAQTIEVAIRALTAVSDQTHIWSVPSIEQGNNDSHAIGLGQMNLHGYLARER
EECCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHH
IFYGSEEGVDFTNIYFYTVLYHALRASNRIAIERGTKFGGFDRSKYASGEFFDKYTDQVW
EEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHC
EPKTEKVRQLFADAGIRIPTQDDWLRLKESVQQHGIYNQNLQAVPPTGSISYINHSTSSI
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCC
HPVASKIEIRKEGKIGRAYYPAPYLTNDNLEYYQDAYEIGYEKIIDTYAAATQHVDQGLS
CHHHHHEEEECCCCCCCEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LTLFFKDTASTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]