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The map label for this gene is ligA

Identifier: 169630426

GI number: 169630426

Start: 3392662

End: 3394707

Strand: Reverse

Name: ligA

Synonym: MAB_3345c

Alternate gene names: 169630426

Gene position: 3394707-3392662 (Counterclockwise)

Preceding gene: 169630432

Following gene: 169630423

Centisome position: 66.99

GC content: 64.32

Gene sequence:

>2046_bases
ATGGATTCCGACATCCAGCGCCAATGGGGCGAGCTGGCCGAAGAGGTGCGCGGCCATCAGTTCCGCTACTACGTCAAGGA
CGCCCCCGTCATTTCTGACGGACAGTTCGACGAGCTGCTACGGCGGCTGACCGCGCTGGAAGAGCAGTACCCCGAGCTGC
GCACACCGGATTCGCCGACGCAGTTGGTCGGCGGCGCGGGATTTGTCACCGAATTCAGATCGGTCGATCACCTGGAGCGA
ATGCTGAGTTTGGACAACGCATTCAGTTCCGATGAATTGACGGCCTGGGATGCCCGGGTGCGTGGCGATATCGGTCAGGA
GCCGGAATACCTGTGCGAGCTGAAGATCGACGGTGTGGCGCTCTCGCTGGTGTACGAGAACGGCGTGCTGGTACGCGGGG
CCACTCGTGGGGACGGGCGTAGCGGTGAGGATGTCACCCTGAACGCCCGGACCATCGAGGACGTGCCGGAAAGGCTTGCG
AAGTCTGAGAAATATCCGATACCCGCGCTCCTGGAGGTGCGCGGTGAAGTGTTCTTCCGGCTGGAGGATTTCGAGGCGCT
GAACGCTTCTCTCGTCGAGGAAAGCAAACCGCCGTTCGCGAACCCTCGTAACAGTGCCGCGGGCAGCCTGCGGCAGAAGA
ACCCGGCGATCACGGCCAGGCGGCGGCTGCGGATGATCTGTCACGGGCTCGGCCGGGCCGAAGGCTTTTCGCCGGAGAGC
TTGCATGATGCGTACTTGGCATTGGGGGAGTGGGGATTACCCGTCTCGACGCACACCACCAAGGTGCGGGGCATTGCCAA
AGTGCAGGAACGTGTCAACTACTGGGCGGAACATCGCCACGACGTGGAACACGAGATCGACGGTGTCGTCGTGAAGGTCG
ACACGGTTGCGCTGCAGCGCCGCCTCGGCAGTACCTCGCGCGCACCCCGCTGGGCTATCGCCTACAAGTACCCGCCGGAG
GAGGCGACAACCGAACTGCTCGACATCCGGGTGAGCGTGGGCCGCACCGGCAGGGTCACCCCGTTCGCGTACATGACTCC
GGTCAAGGTAGCCGGATCGACGGTGTCGTTGGCGACCCTGCACAATGCCTCCGAAGTCAAGCGCAAGGGTGTGCTGATCG
GCGACACGGTGGTGATTCGCAAGGCCGGCGACGTGATTCCCGAGGTGCTGGGTCCGGTTGCCGATCTGAGAAACGGCAAT
GAGCGCGAATTCGTCATGCCCACAGCGTGTCCCGAATGCGGTACCACACTGGCCCACGAGAAGGAGGGCGATGCCGACAT
TCGTTGCCCCAACTCGCGTAGCTGTCCTGCGCAACTGCGGGAGCGGGTGTTCCATGTGGCGGGACGCGGCGCCTTCGATA
TCGAAGCCCTCGGCTATGAGGCGGCAATAGCGCTGCTCGCGGCCGGTGTGATCGAGGATGAGGGGGATCTGTTCGGCCTC
ACCGCTGATGACCTGCTGCGCACCGATCTGTTCAAGACCAAGAGCGGTGCACTGTCCGCCAACGGGGCCCGGCTGCTGGA
CAACCTCGACAAGGCGAAGCAACAGCCGTTGTGGCGGGTGCTGGTGGCGCTTTCGATCCGCCATGTCGGTCCCACCGCGG
CGCGCGCGTTGGCCACCGAATTCGGCGATCTGGAAGCCATCGAGGGTGCCTCCGTCGAACAGTTGGCGGCGGTCGAGGGG
GTAGGGGCCACGATCGCAGCCGCGGTGGTGGATTGGTTCAGCGTCGACTGGCATCGCGCCATCGTCGACAAGTGGCGCGC
CGCGGGCGTGCGGACTGCCGATGAGCGTGACGACTCCATACCGCGCAACCTGGAAGGGTTGTCCATCGTCGTAACCGGTT
CGCTCCCAGGCTTTTCGCGCGACGAGGCCAAGGAGGCCATCATCGCCCGGGGCGGCAAGTCCGCGAGTTCGGTGTCCAAG
AAGACAGCGTTCGTGGTTGTCGGAGATTCGCCGGGATCCAAGTATGACAAGGCCGTTGAGCTCGGGGTGACCATCCTCGA
CGAGGATGGATTCCGGGCCTTGCTGGCCGACGGGCCGCCCGCGTAG

Upstream 100 bases:

>100_bases
GCCAGACCGGGATCGTCCTCGGTGAACATGATGGGGTGCGTGCCCATGGGCACATCGTAGGGTTGGGGCGCTGTCGGGCG
GGTCCGTTAGCCTGCCGGGT

Downstream 100 bases:

>100_bases
CGTGGCGGTGCAGGCGGCCAGATTCTGCCCCGCAACTCTATGATTTCGGCGGAATCGAGAGGTCGAACACTCGCAAGTCG
CCGTAATCCTTTGAAGAGAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MDSDIQRQWGELAEEVRGHQFRYYVKDAPVISDGQFDELLRRLTALEEQYPELRTPDSPTQLVGGAGFVTEFRSVDHLER
MLSLDNAFSSDELTAWDARVRGDIGQEPEYLCELKIDGVALSLVYENGVLVRGATRGDGRSGEDVTLNARTIEDVPERLA
KSEKYPIPALLEVRGEVFFRLEDFEALNASLVEESKPPFANPRNSAAGSLRQKNPAITARRRLRMICHGLGRAEGFSPES
LHDAYLALGEWGLPVSTHTTKVRGIAKVQERVNYWAEHRHDVEHEIDGVVVKVDTVALQRRLGSTSRAPRWAIAYKYPPE
EATTELLDIRVSVGRTGRVTPFAYMTPVKVAGSTVSLATLHNASEVKRKGVLIGDTVVIRKAGDVIPEVLGPVADLRNGN
EREFVMPTACPECGTTLAHEKEGDADIRCPNSRSCPAQLRERVFHVAGRGAFDIEALGYEAAIALLAAGVIEDEGDLFGL
TADDLLRTDLFKTKSGALSANGARLLDNLDKAKQQPLWRVLVALSIRHVGPTAARALATEFGDLEAIEGASVEQLAAVEG
VGATIAAAVVDWFSVDWHRAIVDKWRAAGVRTADERDDSIPRNLEGLSIVVTGSLPGFSRDEAKEAIIARGGKSASSVSK
KTAFVVVGDSPGSKYDKAVELGVTILDEDGFRALLADGPPA

Sequences:

>Translated_681_residues
MDSDIQRQWGELAEEVRGHQFRYYVKDAPVISDGQFDELLRRLTALEEQYPELRTPDSPTQLVGGAGFVTEFRSVDHLER
MLSLDNAFSSDELTAWDARVRGDIGQEPEYLCELKIDGVALSLVYENGVLVRGATRGDGRSGEDVTLNARTIEDVPERLA
KSEKYPIPALLEVRGEVFFRLEDFEALNASLVEESKPPFANPRNSAAGSLRQKNPAITARRRLRMICHGLGRAEGFSPES
LHDAYLALGEWGLPVSTHTTKVRGIAKVQERVNYWAEHRHDVEHEIDGVVVKVDTVALQRRLGSTSRAPRWAIAYKYPPE
EATTELLDIRVSVGRTGRVTPFAYMTPVKVAGSTVSLATLHNASEVKRKGVLIGDTVVIRKAGDVIPEVLGPVADLRNGN
EREFVMPTACPECGTTLAHEKEGDADIRCPNSRSCPAQLRERVFHVAGRGAFDIEALGYEAAIALLAAGVIEDEGDLFGL
TADDLLRTDLFKTKSGALSANGARLLDNLDKAKQQPLWRVLVALSIRHVGPTAARALATEFGDLEAIEGASVEQLAAVEG
VGATIAAAVVDWFSVDWHRAIVDKWRAAGVRTADERDDSIPRNLEGLSIVVTGSLPGFSRDEAKEAIIARGGKSASSVSK
KTAFVVVGDSPGSKYDKAVELGVTILDEDGFRALLADGPPA
>Mature_681_residues
MDSDIQRQWGELAEEVRGHQFRYYVKDAPVISDGQFDELLRRLTALEEQYPELRTPDSPTQLVGGAGFVTEFRSVDHLER
MLSLDNAFSSDELTAWDARVRGDIGQEPEYLCELKIDGVALSLVYENGVLVRGATRGDGRSGEDVTLNARTIEDVPERLA
KSEKYPIPALLEVRGEVFFRLEDFEALNASLVEESKPPFANPRNSAAGSLRQKNPAITARRRLRMICHGLGRAEGFSPES
LHDAYLALGEWGLPVSTHTTKVRGIAKVQERVNYWAEHRHDVEHEIDGVVVKVDTVALQRRLGSTSRAPRWAIAYKYPPE
EATTELLDIRVSVGRTGRVTPFAYMTPVKVAGSTVSLATLHNASEVKRKGVLIGDTVVIRKAGDVIPEVLGPVADLRNGN
EREFVMPTACPECGTTLAHEKEGDADIRCPNSRSCPAQLRERVFHVAGRGAFDIEALGYEAAIALLAAGVIEDEGDLFGL
TADDLLRTDLFKTKSGALSANGARLLDNLDKAKQQPLWRVLVALSIRHVGPTAARALATEFGDLEAIEGASVEQLAAVEG
VGATIAAAVVDWFSVDWHRAIVDKWRAAGVRTADERDDSIPRNLEGLSIVVTGSLPGFSRDEAKEAIIARGGKSASSVSK
KTAFVVVGDSPGSKYDKAVELGVTILDEDGFRALLADGPPA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=77, Percent_Identity=51.9480519480519, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788750, Length=681, Percent_Identity=43.0249632892805, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI87082305, Length=567, Percent_Identity=22.7513227513227, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_MYCA9 (B1MDV2)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704075.1
- ProteinModelPortal:   B1MDV2
- EnsemblBacteria:   EBMYCT00000004138
- GeneID:   5965845
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3345c
- GeneTree:   EBGT00050000016652
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   MABS561007:MAB_3345C-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74083; Mature: 74083

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 176-176 BINDING 292-292 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSDIQRQWGELAEEVRGHQFRYYVKDAPVISDGQFDELLRRLTALEEQYPELRTPDSPT
CCCHHHHHHHHHHHHHCCCEEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCH
QLVGGAGFVTEFRSVDHLERMLSLDNAFSSDELTAWDARVRGDIGQEPEYLCELKIDGVA
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCEEEEEEECCEE
LSLVYENGVLVRGATRGDGRSGEDVTLNARTIEDVPERLAKSEKYPIPALLEVRGEVFFR
EEEEEECCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEE
LEDFEALNASLVEESKPPFANPRNSAAGSLRQKNPAITARRRLRMICHGLGRAEGFSPES
EECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHH
LHDAYLALGEWGLPVSTHTTKVRGIAKVQERVNYWAEHRHDVEHEIDGVVVKVDTVALQR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHHHHHH
RLGSTSRAPRWAIAYKYPPEEATTELLDIRVSVGRTGRVTPFAYMTPVKVAGSTVSLATL
HHCCCCCCCCEEEEEECCCHHHHHHHHHEEEECCCCCCCCCEEEECCHHHCCCEEEEEEC
HNASEVKRKGVLIGDTVVIRKAGDVIPEVLGPVADLRNGNEREFVMPTACPECGTTLAHE
CCHHHHHHCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
KEGDADIRCPNSRSCPAQLRERVFHVAGRGAFDIEALGYEAAIALLAAGVIEDEGDLFGL
CCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEC
TADDLLRTDLFKTKSGALSANGARLLDNLDKAKQQPLWRVLVALSIRHVGPTAARALATE
CHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHH
FGDLEAIEGASVEQLAAVEGVGATIAAAVVDWFSVDWHRAIVDKWRAAGVRTADERDDSI
CCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
PRNLEGLSIVVTGSLPGFSRDEAKEAIIARGGKSASSVSKKTAFVVVGDSPGSKYDKAVE
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCCHHHHHHE
LGVTILDEDGFRALLADGPPA
ECEEEECCCCCEEEECCCCCC
>Mature Secondary Structure
MDSDIQRQWGELAEEVRGHQFRYYVKDAPVISDGQFDELLRRLTALEEQYPELRTPDSPT
CCCHHHHHHHHHHHHHCCCEEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCH
QLVGGAGFVTEFRSVDHLERMLSLDNAFSSDELTAWDARVRGDIGQEPEYLCELKIDGVA
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCEEEEEEECCEE
LSLVYENGVLVRGATRGDGRSGEDVTLNARTIEDVPERLAKSEKYPIPALLEVRGEVFFR
EEEEEECCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEE
LEDFEALNASLVEESKPPFANPRNSAAGSLRQKNPAITARRRLRMICHGLGRAEGFSPES
EECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHH
LHDAYLALGEWGLPVSTHTTKVRGIAKVQERVNYWAEHRHDVEHEIDGVVVKVDTVALQR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHHHHHH
RLGSTSRAPRWAIAYKYPPEEATTELLDIRVSVGRTGRVTPFAYMTPVKVAGSTVSLATL
HHCCCCCCCCEEEEEECCCHHHHHHHHHEEEECCCCCCCCCEEEECCHHHCCCEEEEEEC
HNASEVKRKGVLIGDTVVIRKAGDVIPEVLGPVADLRNGNEREFVMPTACPECGTTLAHE
CCHHHHHHCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
KEGDADIRCPNSRSCPAQLRERVFHVAGRGAFDIEALGYEAAIALLAAGVIEDEGDLFGL
CCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEC
TADDLLRTDLFKTKSGALSANGARLLDNLDKAKQQPLWRVLVALSIRHVGPTAARALATE
CHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHH
FGDLEAIEGASVEQLAAVEGVGATIAAAVVDWFSVDWHRAIVDKWRAAGVRTADERDDSI
CCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
PRNLEGLSIVVTGSLPGFSRDEAKEAIIARGGKSASSVSKKTAFVVVGDSPGSKYDKAVE
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCCHHHHHHE
LGVTILDEDGFRALLADGPPA
ECEEEECCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA