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The map label for this gene is pfkA [H]

Identifier: 169630416

GI number: 169630416

Start: 3382510

End: 3383541

Strand: Reverse

Name: pfkA [H]

Synonym: MAB_3335c

Alternate gene names: 169630416

Gene position: 3383541-3382510 (Counterclockwise)

Preceding gene: 169630417

Following gene: 169630415

Centisome position: 66.77

GC content: 64.15

Gene sequence:

>1032_bases
ATGCGTATCGGAGTTCTCACCGGTGGCGGCGATTGTCCGGGCCTGAATGCGGTGATCCGCGCGGTGGTGCGAACCTGCGC
CAACCGCTACAACTCGCAGGTCGTGGGCTTCCAGGACGGTTGGCGTGGTCTATTGGAGAACCGCAGAATCCACCTTCACA
ACGACGATCGCAATGATCGGCTGCTCACCAAGGGCGGGACGGTGCTCGGTACCGCACGCACCAACCCCGACCGGCTGCGT
GCCGGACTGGATCAGATCAAGCAGACCCTCGACGACAACGGGATCGATGTGCTGATCCCGATCGGCGGAGAAGGCACCCT
CACCGCCGCCAGCTGGCTGGCCGACGAGAACGTTCCCGTGGTCGGGGTGCCCAAGACCATCGATAACGATATCGATTGCA
CCGATGTCACATTCGGATTCGATACGGCGCTGACCATCGCCACCGAGGCAATCGACCGGTTGCACAGCACAGCCGAATCC
CATCAGCGCGTCATGCTGGTCGAGGTGATGGGTCGGCATGCGGGCTGGATCGCGTTGCACGCGGGACTGGCCTCGGGTGC
GCATATGACGCTGATCCCCGAAGTGCCGTTCGATGTGGATGAGGTCTGTCGGCTGATCAAAGGCCGGTTCCAGCGCGGGG
ATTCGCATTTCATCTGTGTGGTGGCCGAGGGCGCCAAGCCGGCCGAGGGCTCAATGGAGCTGCGCGACGGGGGAATTGAC
GAGTTTGGGCATCAGCGATTCACCGGGGTGGCGCAGCAGCTGGGAGTAGAGATCGAGAAGCGCATCAACAAAGAGGTGCG
CACCACGGTGCTGGGGCATGTGCAGCGTGGGGGCACTCCCACACCCTTCGACCGGGTGCTCGCCACCCGATTCGGCGTCA
ACGCCGCCGACGCCGCGCACGCGGGGGAGTTCGGAGTGATGGTGTCGCTGCGCGGCCAAGACATCGGGCGGGTACCACTG
GCGGAAGCGACCCGTCAGCTCAAGCTGGTCCCCAAAAACCGCTACGAGGATGCCGCCGCGTTCTTCGGCTGA

Upstream 100 bases:

>100_bases
CGGCGCACGGTCTGGGACGGGTTCCGGCTGAGCATTCCGAGTCGGATACCGCGGGCCTCGTAGCGCTGCCGCCGGGCGCG
GTCACGGGCATGATGGGGCT

Downstream 100 bases:

>100_bases
GCCGACGCTACCTGCGATTTCTGCGCATAACGTGTCTGTTCGGCCACTCAAGCCGACCTATTAGGATCGCAGCATGACAG
AACTGCTGGACTACGACGAC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCANRYNSQVVGFQDGWRGLLENRRIHLHNDDRNDRLLTKGGTVLGTARTNPDRLR
AGLDQIKQTLDDNGIDVLIPIGGEGTLTAASWLADENVPVVGVPKTIDNDIDCTDVTFGFDTALTIATEAIDRLHSTAES
HQRVMLVEVMGRHAGWIALHAGLASGAHMTLIPEVPFDVDEVCRLIKGRFQRGDSHFICVVAEGAKPAEGSMELRDGGID
EFGHQRFTGVAQQLGVEIEKRINKEVRTTVLGHVQRGGTPTPFDRVLATRFGVNAADAAHAGEFGVMVSLRGQDIGRVPL
AEATRQLKLVPKNRYEDAAAFFG

Sequences:

>Translated_343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCANRYNSQVVGFQDGWRGLLENRRIHLHNDDRNDRLLTKGGTVLGTARTNPDRLR
AGLDQIKQTLDDNGIDVLIPIGGEGTLTAASWLADENVPVVGVPKTIDNDIDCTDVTFGFDTALTIATEAIDRLHSTAES
HQRVMLVEVMGRHAGWIALHAGLASGAHMTLIPEVPFDVDEVCRLIKGRFQRGDSHFICVVAEGAKPAEGSMELRDGGID
EFGHQRFTGVAQQLGVEIEKRINKEVRTTVLGHVQRGGTPTPFDRVLATRFGVNAADAAHAGEFGVMVSLRGQDIGRVPL
AEATRQLKLVPKNRYEDAAAFFG
>Mature_343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCANRYNSQVVGFQDGWRGLLENRRIHLHNDDRNDRLLTKGGTVLGTARTNPDRLR
AGLDQIKQTLDDNGIDVLIPIGGEGTLTAASWLADENVPVVGVPKTIDNDIDCTDVTFGFDTALTIATEAIDRLHSTAES
HQRVMLVEVMGRHAGWIALHAGLASGAHMTLIPEVPFDVDEVCRLIKGRFQRGDSHFICVVAEGAKPAEGSMELRDGGID
EFGHQRFTGVAQQLGVEIEKRINKEVRTTVLGHVQRGGTPTPFDRVLATRFGVNAADAAHAGEFGVMVSLRGQDIGRVPL
AEATRQLKLVPKNRYEDAAAFFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=360, Percent_Identity=37.5, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI266453748, Length=360, Percent_Identity=37.5, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI4505749, Length=360, Percent_Identity=37.5, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI266453619, Length=360, Percent_Identity=37.5, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI48762920, Length=375, Percent_Identity=34.4, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI11321601, Length=375, Percent_Identity=34.9333333333333, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1790350, Length=338, Percent_Identity=39.0532544378698, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25147584, Length=361, Percent_Identity=32.409972299169, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558788, Length=363, Percent_Identity=32.2314049586777, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI72003601, Length=320, Percent_Identity=28.4375, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321679, Length=366, Percent_Identity=35.792349726776, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323861, Length=361, Percent_Identity=32.1329639889197, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI28573326, Length=375, Percent_Identity=36.5333333333333, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24652337, Length=375, Percent_Identity=36.8, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI17647809, Length=375, Percent_Identity=36.8, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37098; Mature: 37098

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTCANRYNSQVVGFQDGWRGLLENRRIHLHNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCEEEEECCCCCCE
LLTKGGTVLGTARTNPDRLRAGLDQIKQTLDDNGIDVLIPIGGEGTLTAASWLADENVPV
EEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCE
VGVPKTIDNDIDCTDVTFGFDTALTIATEAIDRLHSTAESHQRVMLVEVMGRHAGWIALH
EECCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEEHHCCCCCEEEEE
AGLASGAHMTLIPEVPFDVDEVCRLIKGRFQRGDSHFICVVAEGAKPAEGSMELRDGGID
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEECCCCCC
EFGHQRFTGVAQQLGVEIEKRINKEVRTTVLGHVQRGGTPTPFDRVLATRFGVNAADAAH
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
AGEFGVMVSLRGQDIGRVPLAEATRQLKLVPKNRYEDAAAFFG
CCCEEEEEEECCCCCCCCCHHHHHHHEEECCCCCCCCHHHHCC
>Mature Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTCANRYNSQVVGFQDGWRGLLENRRIHLHNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCEEEEECCCCCCE
LLTKGGTVLGTARTNPDRLRAGLDQIKQTLDDNGIDVLIPIGGEGTLTAASWLADENVPV
EEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCCE
VGVPKTIDNDIDCTDVTFGFDTALTIATEAIDRLHSTAESHQRVMLVEVMGRHAGWIALH
EECCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEEEHHCCCCCEEEEE
AGLASGAHMTLIPEVPFDVDEVCRLIKGRFQRGDSHFICVVAEGAKPAEGSMELRDGGID
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEECCCCCC
EFGHQRFTGVAQQLGVEIEKRINKEVRTTVLGHVQRGGTPTPFDRVLATRFGVNAADAAH
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
AGEFGVMVSLRGQDIGRVPLAEATRQLKLVPKNRYEDAAAFFG
CCCEEEEEEECCCCCCCCCHHHHHHHEEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]