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The map label for this gene is gatB [H]

Identifier: 169630415

GI number: 169630415

Start: 3380937

End: 3382436

Strand: Reverse

Name: gatB [H]

Synonym: MAB_3334c

Alternate gene names: 169630415

Gene position: 3382436-3380937 (Counterclockwise)

Preceding gene: 169630416

Following gene: 169630413

Centisome position: 66.75

GC content: 66.07

Gene sequence:

>1500_bases
ATGACAGAACTGCTGGACTACGACGACGTGCTGACCCGATACGAGCCGGTGCTCGGTATGGAGGTTCACGTCGAACTGTC
CACGGCCACCAAGATGTTCTGTGGCTGCCCGACCACCTTCGGCGCGGAACCCAATACCCAGATTTGCCCCGTCTGCCTGG
GTCTGCCGGGCTCGCTGCCGGTCGTCAACGAAAAGGCCGTCGAATCCGCCATCCGGATCGGTCTGGCGCTGAACTGCGAG
ATCGCTCCCTGGGGCCGATTCGCCCGCAAGAACTATTTCTACCCGGATCAGCCGAAGAACTATCAGATCTCCCAGTACGA
CGAGCCGATCGCGTTCAACGGCTACCTCGATGTTCCGCTGGAAGACGGCACCACGTGGCGGGTGCATATCGAGCGCGCGC
ACATGGAAGAGGACACCGGCAAGCTGACCCACATCGGTAGCGAGACGGGCCGCATCTCCGGCGCCACCGAGTCGCTGCTC
GACTACAACCGTGCGGGTGTGCCGCTGGTCGAGATCGTCACCAAACCTATCGAGGGGACGGGGCAGCGCGCCCCGGAGAT
CGCCCGCGCCTACGTGACGGCATTGCGGGATCTGTTGCGCGCCTTGGATGTCTCCGATGTGCGCATGGACCATGGGTCGA
TGCGCTGCGACGCGAATGTGTCGCTGATGCCCACCGGCGCCGGTGAGTTCGGAACCCGGACCGAGACCAAGAACGTCAAC
TCGCTCAAGAGCGTCGAGGTGGCGGTGCGTTACGAGATGCGGCGCCAGGCAGCGGTTCTAGACGCGGGCGAGGACGTCAT
TCAGGAGACGCGGCACTTCCTCGAGCAGGACGGCTCCACCAGCGCCGGACGCCGCAAGGAGACCGCCGAGGACTACCGGT
ACTTCCCGGAGCCCGACCTCGAGCCCGTAGCGCCCAGCGCGGAGCTCATCGAGAAGCTGCGCGGCACCCTCCCGGAGCTG
CCCTGGCTGCGGCTGGGCCGTATCCAGCAGGAGTGGGGCGTCTCCGACGAGGTGATGCGCGACCTGATCAACAACGGGGC
GGTCGAACTGGTGCAGGCCACCGTCGCCGAGGGCGCCAGCAGTGAGGACGCACGCTCCTGGTGGGGCAACTACCTGGTGC
AGCAGGCCAACTCGCGTGAGGTTGAACTGGCGGACCTGCCCATCACGCCCACTCAGGTGGCCGCCGTGGTCGCGTTGATC
AAGGACGGCAAGCTGTCCAACAAGTTGGCGCGGCAGGTCGTCGACGGCGTACTCGCCGGTGAGGGCGAGCCCGAGCAGGT
GATGAAGGATCGCGGTCTGGTCGTGGTGCGCGACGACTCGGTGATCCAGGCTGCTGTCGATGAGGCGCTGGCCGCCAACC
CCGATGTCGCCCAGAAGATCCGCGATGGCAAGGTCGCCGCCGCGGGCGCCATTGTCGGCGCGGTGATGAAGGCCACCAAG
GGTCAGGCCGACGCCGCGCTGGTCAAGGACTTGGTGCTCAAGGCCTGTGGCCAGGCCTAG

Upstream 100 bases:

>100_bases
GAGGATGCCGCCGCGTTCTTCGGCTGAGCCGACGCTACCTGCGATTTCTGCGCATAACGTGTCTGTTCGGCCACTCAAGC
CGACCTATTAGGATCGCAGC

Downstream 100 bases:

>100_bases
TTAGTCCAGGTCGTCGTTGGCGCGGGGGAATCCGCCGCCCTTGGGGAACAGTGGGAAGACCACGTCCTCGGTGTCGGTGG
CGGTACCGAAGTCCTTGTTG

Product: aspartyl/glutamyl-tRNA amidotransferase, B subunit (GatB)

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTELLDYDDVLTRYEPVLGMEVHVELSTATKMFCGCPTTFGAEPNTQICPVCLGLPGSLPVVNEKAVESAIRIGLALNCE
IAPWGRFARKNYFYPDQPKNYQISQYDEPIAFNGYLDVPLEDGTTWRVHIERAHMEEDTGKLTHIGSETGRISGATESLL
DYNRAGVPLVEIVTKPIEGTGQRAPEIARAYVTALRDLLRALDVSDVRMDHGSMRCDANVSLMPTGAGEFGTRTETKNVN
SLKSVEVAVRYEMRRQAAVLDAGEDVIQETRHFLEQDGSTSAGRRKETAEDYRYFPEPDLEPVAPSAELIEKLRGTLPEL
PWLRLGRIQQEWGVSDEVMRDLINNGAVELVQATVAEGASSEDARSWWGNYLVQQANSREVELADLPITPTQVAAVVALI
KDGKLSNKLARQVVDGVLAGEGEPEQVMKDRGLVVVRDDSVIQAAVDEALAANPDVAQKIRDGKVAAAGAIVGAVMKATK
GQADAALVKDLVLKACGQA

Sequences:

>Translated_499_residues
MTELLDYDDVLTRYEPVLGMEVHVELSTATKMFCGCPTTFGAEPNTQICPVCLGLPGSLPVVNEKAVESAIRIGLALNCE
IAPWGRFARKNYFYPDQPKNYQISQYDEPIAFNGYLDVPLEDGTTWRVHIERAHMEEDTGKLTHIGSETGRISGATESLL
DYNRAGVPLVEIVTKPIEGTGQRAPEIARAYVTALRDLLRALDVSDVRMDHGSMRCDANVSLMPTGAGEFGTRTETKNVN
SLKSVEVAVRYEMRRQAAVLDAGEDVIQETRHFLEQDGSTSAGRRKETAEDYRYFPEPDLEPVAPSAELIEKLRGTLPEL
PWLRLGRIQQEWGVSDEVMRDLINNGAVELVQATVAEGASSEDARSWWGNYLVQQANSREVELADLPITPTQVAAVVALI
KDGKLSNKLARQVVDGVLAGEGEPEQVMKDRGLVVVRDDSVIQAAVDEALAANPDVAQKIRDGKVAAAGAIVGAVMKATK
GQADAALVKDLVLKACGQA
>Mature_498_residues
TELLDYDDVLTRYEPVLGMEVHVELSTATKMFCGCPTTFGAEPNTQICPVCLGLPGSLPVVNEKAVESAIRIGLALNCEI
APWGRFARKNYFYPDQPKNYQISQYDEPIAFNGYLDVPLEDGTTWRVHIERAHMEEDTGKLTHIGSETGRISGATESLLD
YNRAGVPLVEIVTKPIEGTGQRAPEIARAYVTALRDLLRALDVSDVRMDHGSMRCDANVSLMPTGAGEFGTRTETKNVNS
LKSVEVAVRYEMRRQAAVLDAGEDVIQETRHFLEQDGSTSAGRRKETAEDYRYFPEPDLEPVAPSAELIEKLRGTLPELP
WLRLGRIQQEWGVSDEVMRDLINNGAVELVQATVAEGASSEDARSWWGNYLVQQANSREVELADLPITPTQVAAVVALIK
DGKLSNKLARQVVDGVLAGEGEPEQVMKDRGLVVVRDDSVIQAAVDEALAANPDVAQKIRDGKVAAAGAIVGAVMKATKG
QADAALVKDLVLKACGQA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=507, Percent_Identity=32.741617357002, Blast_Score=266, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI115534094, Length=294, Percent_Identity=40.1360544217687, Blast_Score=201, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=313, Percent_Identity=32.9073482428115, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=28.7449392712551, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54357; Mature: 54226

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELLDYDDVLTRYEPVLGMEVHVELSTATKMFCGCPTTFGAEPNTQICPVCLGLPGSLP
CCCCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
VVNEKAVESAIRIGLALNCEIAPWGRFARKNYFYPDQPKNYQISQYDEPIAFNGYLDVPL
CCCHHHHHHHHHHCEEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEC
EDGTTWRVHIERAHMEEDTGKLTHIGSETGRISGATESLLDYNRAGVPLVEIVTKPIEGT
CCCCEEEEEEEEHHCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCC
GQRAPEIARAYVTALRDLLRALDVSDVRMDHGSMRCDANVSLMPTGAGEFGTRTETKNVN
CCCCHHHHHHHHHHHHHHHHHCCHHHHEECCCCEEECCCCEEEECCCCCCCCCCCCCCCC
SLKSVEVAVRYEMRRQAAVLDAGEDVIQETRHFLEQDGSTSAGRRKETAEDYRYFPEPDL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCC
EPVAPSAELIEKLRGTLPELPWLRLGRIQQEWGVSDEVMRDLINNGAVELVQATVAEGAS
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCC
SEDARSWWGNYLVQQANSREVELADLPITPTQVAAVVALIKDGKLSNKLARQVVDGVLAG
CHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
EGEPEQVMKDRGLVVVRDDSVIQAAVDEALAANPDVAQKIRDGKVAAAGAIVGAVMKATK
CCCHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCC
GQADAALVKDLVLKACGQA
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TELLDYDDVLTRYEPVLGMEVHVELSTATKMFCGCPTTFGAEPNTQICPVCLGLPGSLP
CCCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
VVNEKAVESAIRIGLALNCEIAPWGRFARKNYFYPDQPKNYQISQYDEPIAFNGYLDVPL
CCCHHHHHHHHHHCEEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEC
EDGTTWRVHIERAHMEEDTGKLTHIGSETGRISGATESLLDYNRAGVPLVEIVTKPIEGT
CCCCEEEEEEEEHHCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCC
GQRAPEIARAYVTALRDLLRALDVSDVRMDHGSMRCDANVSLMPTGAGEFGTRTETKNVN
CCCCHHHHHHHHHHHHHHHHHCCHHHHEECCCCEEECCCCEEEECCCCCCCCCCCCCCCC
SLKSVEVAVRYEMRRQAAVLDAGEDVIQETRHFLEQDGSTSAGRRKETAEDYRYFPEPDL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCC
EPVAPSAELIEKLRGTLPELPWLRLGRIQQEWGVSDEVMRDLINNGAVELVQATVAEGAS
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCC
SEDARSWWGNYLVQQANSREVELADLPITPTQVAAVVALIKDGKLSNKLARQVVDGVLAG
CHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
EGEPEQVMKDRGLVVVRDDSVIQAAVDEALAANPDVAQKIRDGKVAAAGAIVGAVMKATK
CCCHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCC
GQADAALVKDLVLKACGQA
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA