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The map label for this gene is gltX

Identifier: 169630379

GI number: 169630379

Start: 3336060

End: 3337550

Strand: Reverse

Name: gltX

Synonym: MAB_3298c

Alternate gene names: 169630379

Gene position: 3337550-3336060 (Counterclockwise)

Preceding gene: 169630380

Following gene: 169630375

Centisome position: 65.87

GC content: 64.39

Gene sequence:

>1491_bases
GTGAGTAACACGAAAATCCGGGTACGTTTCTGCCCGTCGCCGACGGGCACGCCGCACGTCGGCCTGATTCGCACCGCACT
GTTCAACTGGGCGTATGCGCGACACTGCGGTGGGGACTTCGTGTTCCGCATCGAAGACACCGATGCCGGACGCGACAGTG
AGGAGAGCTACCTGGCGATCCTGGATGCCTTGCGATGGCTGGGGCTGGACTGGGACGAAGGGCCGGAGGTCGGCGGACCC
TACGAGCCCTACCGACAGTCACAGCGTAAGGAGCTGCATCGGGAGGTGGTGCGCAAGCTCCTGGAGGCGGGGGAGGCCTA
TGAGGCATTCTCCACGGCGGAGGAAGTCGAAGCCCGCCATCTTGCCGCGGGACGCAATCCCAAACTGGGCTATGACAACT
ATGACCGTGATCTCACCGAGGAGCAGCGCGCGGCATTCCGTGCGGAGGGACGCGATCCCGTCGTCCGGCTGCGGATGCCC
GATCACGACATCACCTGGAACGACTTGGTGCGCGGCGAGACCACCTTCGCGGCGGGTGTGGTGCCTGATTTTGCGCTGAC
CCGCGGTAACGGGGATCCGTTGTACACCTTGGTGAACCCGGTCGACGACGCGTTGATGAAGATCACCCATGTGCTGCGCG
GCGAGGACCTGCTGCCCTCGACACCGCGGCAGATCGCTTTGTACGAGGCCATGATCCGGATCGGAGTGACCGACTCGATA
CCGGCCTTCGCGCACCTGCCGTCGGTGCTGGGGGAGGGCACGAAGAAGCTGTCCAAGCGTGATCCGCAGTCCAACCTGTT
TCTTCATCGCGATCGCGGATTCATTCCCGAGGGTCTGCTGAATTACCTTGCCCTGCTGGGGTGGTCGATCGCCGATGATC
ACGACATCTTCTCACTTGCCGAGATGGTCACCGCCTTCGATGTCACCGATGTCAACTCGAATCCGGCGCGATTCGACCAG
AAGAAGGCCGATGCCATCAACGCCGAGCACATCCGGCTGTTGGCGCCGGAGGAGTTCGTGACCCGGCTGCGCACGTACTT
CGCCGCGCATGGGCACGAGCTCGGGCTCGACGAGAGCGCCTTCGCGGTGGCGGCGGATCTGGTGCAGACGCGCATCGTGG
TGCTGGGCGACGCCTGGGGGCTGCTGAAGTTCCTCAACGACGACGCGTACGCGATCGACCCGGGCGCGGCCCGCAAGGAA
CTCGGAGAGGCCTCGTTGCCTGTCCTGGACGCCGCAATCGGTGCGCTGGAGTCACTCGAGGCGTGGACCACCCCTGCCAT
CGAGGGTGCGCTCAAGAGCGCACTCATCGAAGGGCTCGAGCTCAAACCGCGGAAGGCTTTCGGCCCGCTGCGTGTCGCGG
TTACGGGTGCAACGGTGAGCCCGCCGCTGTTCGAATCGATGGAGCTGCTGGGTTCCGGGCGCACCCTGACACGGCTGAGA
AACGCTCGGAGCTGGGAAAATGGGGTGGGTGACCAGACCGATTCGGGTTAA

Upstream 100 bases:

>100_bases
CCGAGGGCGTCGGGCCCATTGTCGACGGGGACACCGTGAGCGTCACTATCGAAGGTATCGGAACCCTGTCCAATCCCGTT
GTGCGGAAGGCGAAGTAAGA

Downstream 100 bases:

>100_bases
TGGCCCTTGGTTTGGTACTCTGCTCGTCGGCTCCAAAGTCCGCTATCAGCTCGCTGACCTGCGGTTCTGCGAAGCCATTG
GGGTATGGTGTAATTGGCAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSNTKIRVRFCPSPTGTPHVGLIRTALFNWAYARHCGGDFVFRIEDTDAGRDSEESYLAILDALRWLGLDWDEGPEVGGP
YEPYRQSQRKELHREVVRKLLEAGEAYEAFSTAEEVEARHLAAGRNPKLGYDNYDRDLTEEQRAAFRAEGRDPVVRLRMP
DHDITWNDLVRGETTFAAGVVPDFALTRGNGDPLYTLVNPVDDALMKITHVLRGEDLLPSTPRQIALYEAMIRIGVTDSI
PAFAHLPSVLGEGTKKLSKRDPQSNLFLHRDRGFIPEGLLNYLALLGWSIADDHDIFSLAEMVTAFDVTDVNSNPARFDQ
KKADAINAEHIRLLAPEEFVTRLRTYFAAHGHELGLDESAFAVAADLVQTRIVVLGDAWGLLKFLNDDAYAIDPGAARKE
LGEASLPVLDAAIGALESLEAWTTPAIEGALKSALIEGLELKPRKAFGPLRVAVTGATVSPPLFESMELLGSGRTLTRLR
NARSWENGVGDQTDSG

Sequences:

>Translated_496_residues
MSNTKIRVRFCPSPTGTPHVGLIRTALFNWAYARHCGGDFVFRIEDTDAGRDSEESYLAILDALRWLGLDWDEGPEVGGP
YEPYRQSQRKELHREVVRKLLEAGEAYEAFSTAEEVEARHLAAGRNPKLGYDNYDRDLTEEQRAAFRAEGRDPVVRLRMP
DHDITWNDLVRGETTFAAGVVPDFALTRGNGDPLYTLVNPVDDALMKITHVLRGEDLLPSTPRQIALYEAMIRIGVTDSI
PAFAHLPSVLGEGTKKLSKRDPQSNLFLHRDRGFIPEGLLNYLALLGWSIADDHDIFSLAEMVTAFDVTDVNSNPARFDQ
KKADAINAEHIRLLAPEEFVTRLRTYFAAHGHELGLDESAFAVAADLVQTRIVVLGDAWGLLKFLNDDAYAIDPGAARKE
LGEASLPVLDAAIGALESLEAWTTPAIEGALKSALIEGLELKPRKAFGPLRVAVTGATVSPPLFESMELLGSGRTLTRLR
NARSWENGVGDQTDSG
>Mature_495_residues
SNTKIRVRFCPSPTGTPHVGLIRTALFNWAYARHCGGDFVFRIEDTDAGRDSEESYLAILDALRWLGLDWDEGPEVGGPY
EPYRQSQRKELHREVVRKLLEAGEAYEAFSTAEEVEARHLAAGRNPKLGYDNYDRDLTEEQRAAFRAEGRDPVVRLRMPD
HDITWNDLVRGETTFAAGVVPDFALTRGNGDPLYTLVNPVDDALMKITHVLRGEDLLPSTPRQIALYEAMIRIGVTDSIP
AFAHLPSVLGEGTKKLSKRDPQSNLFLHRDRGFIPEGLLNYLALLGWSIADDHDIFSLAEMVTAFDVTDVNSNPARFDQK
KADAINAEHIRLLAPEEFVTRLRTYFAAHGHELGLDESAFAVAADLVQTRIVVLGDAWGLLKFLNDDAYAIDPGAARKEL
GEASLPVLDAAIGALESLEAWTTPAIEGALKSALIEGLELKPRKAFGPLRVAVTGATVSPPLFESMELLGSGRTLTRLRN
ARSWENGVGDQTDSG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=499, Percent_Identity=34.2685370741483, Blast_Score=213, Evalue=4e-55,
Organism=Escherichia coli, GI1788743, Length=485, Percent_Identity=35.4639175257732, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI226510925, Length=265, Percent_Identity=30.5660377358491, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=28.659793814433, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6324540, Length=347, Percent_Identity=36.5994236311239, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=31.7269076305221, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_MYCA9 (B1MDQ5)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704028.1
- ProteinModelPortal:   B1MDQ5
- SMR:   B1MDQ5
- EnsemblBacteria:   EBMYCT00000007050
- GeneID:   5967485
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3298c
- GeneTree:   EBGT00050000016746
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- BioCyc:   MABS561007:MAB_3298C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54711; Mature: 54580

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTKIRVRFCPSPTGTPHVGLIRTALFNWAYARHCGGDFVFRIEDTDAGRDSEESYLAI
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
LDALRWLGLDWDEGPEVGGPYEPYRQSQRKELHREVVRKLLEAGEAYEAFSTAEEVEARH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LAAGRNPKLGYDNYDRDLTEEQRAAFRAEGRDPVVRLRMPDHDITWNDLVRGETTFAAGV
HHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCEECCC
VPDFALTRGNGDPLYTLVNPVDDALMKITHVLRGEDLLPSTPRQIALYEAMIRIGVTDSI
CCCCEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PAFAHLPSVLGEGTKKLSKRDPQSNLFLHRDRGFIPEGLLNYLALLGWSIADDHDIFSLA
CHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
EMVTAFDVTDVNSNPARFDQKKADAINAEHIRLLAPEEFVTRLRTYFAAHGHELGLDESA
HHHHHHCEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCHHH
FAVAADLVQTRIVVLGDAWGLLKFLNDDAYAIDPGAARKELGEASLPVLDAAIGALESLE
HHHHHHHHHHEEEEEECHHHHHHHHCCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHH
AWTTPAIEGALKSALIEGLELKPRKAFGPLRVAVTGATVSPPLFESMELLGSGRTLTRLR
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHH
NARSWENGVGDQTDSG
HHCCHHCCCCCCCCCC
>Mature Secondary Structure 
SNTKIRVRFCPSPTGTPHVGLIRTALFNWAYARHCGGDFVFRIEDTDAGRDSEESYLAI
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
LDALRWLGLDWDEGPEVGGPYEPYRQSQRKELHREVVRKLLEAGEAYEAFSTAEEVEARH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LAAGRNPKLGYDNYDRDLTEEQRAAFRAEGRDPVVRLRMPDHDITWNDLVRGETTFAAGV
HHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCEECCC
VPDFALTRGNGDPLYTLVNPVDDALMKITHVLRGEDLLPSTPRQIALYEAMIRIGVTDSI
CCCCEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PAFAHLPSVLGEGTKKLSKRDPQSNLFLHRDRGFIPEGLLNYLALLGWSIADDHDIFSLA
CHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
EMVTAFDVTDVNSNPARFDQKKADAINAEHIRLLAPEEFVTRLRTYFAAHGHELGLDESA
HHHHHHCEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCHHH
FAVAADLVQTRIVVLGDAWGLLKFLNDDAYAIDPGAARKELGEASLPVLDAAIGALESLE
HHHHHHHHHHEEEEEECHHHHHHHHCCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHH
AWTTPAIEGALKSALIEGLELKPRKAFGPLRVAVTGATVSPPLFESMELLGSGRTLTRLR
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHH
NARSWENGVGDQTDSG
HHCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA