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The map label for this gene is leuC

Identifier: 169630375

GI number: 169630375

Start: 3332708

End: 3334150

Strand: Reverse

Name: leuC

Synonym: MAB_3294c

Alternate gene names: 169630375

Gene position: 3334150-3332708 (Counterclockwise)

Preceding gene: 169630379

Following gene: 169630374

Centisome position: 65.8

GC content: 64.59

Gene sequence:

>1443_bases
ATGACTAGTGTCCAACAGCCCAGGACCCTGGCAGAGAAGGTCTGGGACTCCCACGTAGTGGTACGCGGAACCGGTGAGGG
TGAGGCGCGCGAGCCCGATCTCATCTATATCGACCTGCACTTGGTGCACGAGGTGACCAGTCCGCAGGCATTCGATGGTC
TTCGGCTCGCTGGGCGCCAGGTGCGCCGGCCGGACCTGACGATCGCGACCGAAGACCACAATGTGCCGACGATCGACATC
GACAAGCCGATCGCCGATCCGGTGTCCCGCACCCAGGTTGAGACCCTTCGGAAGAACTGCGAAGAATTCGGCATCCGCCT
GCATCCCATGGGGGATGCCGAGCAGGGCATCGTGCACGTGGTCGGTCCGCAGCTGGGATTGACGCAGCCGGGTACCACGG
TGGTGTGTGGCGACAGCCACACCTCCACGCATGGCGCGTTCGGGGCGCTGGCGATGGGTATTGGGACGTCCGAAGTGGAG
CATGTGCTGGCCACGCAGACCTTGCCGTTGAGGCCATTTCGGACCATGGCGATCAACATCGATGGCAGCCTGCCGACCGG
TGTCACCAGCAAGGACGTTATTTTGGCCGTCATCGCCAAGATCGGCACCGGCGGGGGGCAGGGGTATGTCCTGGAGTATC
GCGGGCAGGCCGTCGAGGAGATGTCGATGGAGGCCCGAATGACTATGTGCAATATGTCTATTGAGGCGGGTGCCCGGGCG
GGAATGGTCGCGCCCGACGAGACGACATATGCCTATCTGAAGGACCGTCCGCACGCCCCCAAGGGGCAGCTGTGGGATGC
CGCTGTGGCCGAATGGGACTCGTTGCGCACCGACGAGGGTGCCCAGTTCGACGCCGAAGTACACATTGATGCCAGTACTT
TGACGCCCTTCGTCACCTGGGGCACCAATCCCGGGCAGGGGGTGCCTCTGGGCGCTGACGTGCCCGATCCTGAGCTGATG
GCCGACGAGGAAGAGCGGCTGGCGGCCGAGAAGGCGCTGACCTACATGGATCTCACTCCGGGTACCCCCATGCGTGAGAT
CCCGGTGGACACCGTGTTCGTCGGGTCCTGCACCAACGGGCGGATCGAGGACCTACGGGCCGTCGCGGACGTGCTGCGGG
ACCGGAAGGTGGCCGCAGGGGTCACCATGCTCATCGTTCCCGGCTCGATGAGGGTGCGTGCACAGGCCGAATCCGAGGGG
CTGGGCGAGATTTTCGCCGCCGCCGGTGCCCAATGGCGCCAGGCGGGCTGTTCGATGTGCCTGGGCATGAACCCCGACCA
GCTTTCCCCGGGTCAGCGGTGTGCGTCGACGTCGAACCGAAATTTCGAAGGACGGCAGGGTAAGGGCGGTCGCACCCACC
TGGTATCGCCGGCCGTCGCCGCGGCCACGGCGGTGAGGGGAAAGCTCTCATCGCCAGCTGATTTGGCAAAATCCGTAAAA
TAG

Upstream 100 bases:

>100_bases
GACCTGCACCGCTTTGTCGAGGACGCCGATGCCGCTATGCTGTCTCACGTAACGATACTAACGTTCCAAATATTGAGACG
CAATCAGCACGGAGCCAATG

Downstream 100 bases:

>100_bases
GCACCTGCCAAAAGGCACAGGAGGAAATAGTGGAGGCATTCGACACCCACACCGGAATTGGTGTGCCGCTGCGCCGTTCC
AACGTGGACACCGACCAGAT

Product: 3-isopropylmalate dehydratase, large subunit (Leu2)

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTSVQQPRTLAEKVWDSHVVVRGTGEGEAREPDLIYIDLHLVHEVTSPQAFDGLRLAGRQVRRPDLTIATEDHNVPTIDI
DKPIADPVSRTQVETLRKNCEEFGIRLHPMGDAEQGIVHVVGPQLGLTQPGTTVVCGDSHTSTHGAFGALAMGIGTSEVE
HVLATQTLPLRPFRTMAINIDGSLPTGVTSKDVILAVIAKIGTGGGQGYVLEYRGQAVEEMSMEARMTMCNMSIEAGARA
GMVAPDETTYAYLKDRPHAPKGQLWDAAVAEWDSLRTDEGAQFDAEVHIDASTLTPFVTWGTNPGQGVPLGADVPDPELM
ADEEERLAAEKALTYMDLTPGTPMREIPVDTVFVGSCTNGRIEDLRAVADVLRDRKVAAGVTMLIVPGSMRVRAQAESEG
LGEIFAAAGAQWRQAGCSMCLGMNPDQLSPGQRCASTSNRNFEGRQGKGGRTHLVSPAVAAATAVRGKLSSPADLAKSVK

Sequences:

>Translated_480_residues
MTSVQQPRTLAEKVWDSHVVVRGTGEGEAREPDLIYIDLHLVHEVTSPQAFDGLRLAGRQVRRPDLTIATEDHNVPTIDI
DKPIADPVSRTQVETLRKNCEEFGIRLHPMGDAEQGIVHVVGPQLGLTQPGTTVVCGDSHTSTHGAFGALAMGIGTSEVE
HVLATQTLPLRPFRTMAINIDGSLPTGVTSKDVILAVIAKIGTGGGQGYVLEYRGQAVEEMSMEARMTMCNMSIEAGARA
GMVAPDETTYAYLKDRPHAPKGQLWDAAVAEWDSLRTDEGAQFDAEVHIDASTLTPFVTWGTNPGQGVPLGADVPDPELM
ADEEERLAAEKALTYMDLTPGTPMREIPVDTVFVGSCTNGRIEDLRAVADVLRDRKVAAGVTMLIVPGSMRVRAQAESEG
LGEIFAAAGAQWRQAGCSMCLGMNPDQLSPGQRCASTSNRNFEGRQGKGGRTHLVSPAVAAATAVRGKLSSPADLAKSVK
>Mature_479_residues
TSVQQPRTLAEKVWDSHVVVRGTGEGEAREPDLIYIDLHLVHEVTSPQAFDGLRLAGRQVRRPDLTIATEDHNVPTIDID
KPIADPVSRTQVETLRKNCEEFGIRLHPMGDAEQGIVHVVGPQLGLTQPGTTVVCGDSHTSTHGAFGALAMGIGTSEVEH
VLATQTLPLRPFRTMAINIDGSLPTGVTSKDVILAVIAKIGTGGGQGYVLEYRGQAVEEMSMEARMTMCNMSIEAGARAG
MVAPDETTYAYLKDRPHAPKGQLWDAAVAEWDSLRTDEGAQFDAEVHIDASTLTPFVTWGTNPGQGVPLGADVPDPELMA
DEEERLAAEKALTYMDLTPGTPMREIPVDTVFVGSCTNGRIEDLRAVADVLRDRKVAAGVTMLIVPGSMRVRAQAESEGL
GEIFAAAGAQWRQAGCSMCLGMNPDQLSPGQRCASTSNRNFEGRQGKGGRTHLVSPAVAAATAVRGKLSSPADLAKSVK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=27.4725274725275, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI8659555, Length=461, Percent_Identity=24.7288503253796, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=24.1847826086957, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=60.0431965442765, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI87081781, Length=361, Percent_Identity=24.9307479224377, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=24.4444444444444, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367097, Length=401, Percent_Identity=25.9351620947631, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=362, Percent_Identity=30.1104972375691, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=362, Percent_Identity=30.1104972375691, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=316, Percent_Identity=30.0632911392405, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=396, Percent_Identity=24.2424242424242, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=464, Percent_Identity=57.5431034482759, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6320440, Length=455, Percent_Identity=27.4725274725275, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=397, Percent_Identity=26.9521410579345, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=371, Percent_Identity=27.4932614555256, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281365315, Length=494, Percent_Identity=25.9109311740891, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=494, Percent_Identity=25.9109311740891, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=25.9541984732824, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571643, Length=399, Percent_Identity=27.3182957393484, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24645686, Length=375, Percent_Identity=25.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=25.2032520325203, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_MYCA9 (B1MDQ1)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704024.1
- EnsemblBacteria:   EBMYCT00000004751
- GeneID:   5965794
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3294c
- GeneTree:   EBGT00050000014567
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- BioCyc:   MABS561007:MAB_3294C-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51217; Mature: 51085

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVQQPRTLAEKVWDSHVVVRGTGEGEAREPDLIYIDLHLVHEVTSPQAFDGLRLAGRQ
CCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCHHCCHHHHCHH
VRRPDLTIATEDHNVPTIDIDKPIADPVSRTQVETLRKNCEEFGIRLHPMGDAEQGIVHV
CCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCCEEEE
VGPQLGLTQPGTTVVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFRTMAINI
ECCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEE
DGSLPTGVTSKDVILAVIAKIGTGGGQGYVLEYRGQAVEEMSMEARMTMCNMSIEAGARA
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHCCCCC
GMVAPDETTYAYLKDRPHAPKGQLWDAAVAEWDSLRTDEGAQFDAEVHIDASTLTPFVTW
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCEEEEE
GTNPGQGVPLGADVPDPELMADEEERLAAEKALTYMDLTPGTPMREIPVDTVFVGSCTNG
CCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHEEEECCCCCCHHHCCCCEEEEECCCCC
RIEDLRAVADVLRDRKVAAGVTMLIVPGSMRVRAQAESEGLGEIFAAAGAQWRQAGCSMC
HHHHHHHHHHHHHCCHHHCCEEEEEECCCEEEEEECCCCCHHHHHHHCCHHHHHHCCCEE
LGMNPDQLSPGQRCASTSNRNFEGRQGKGGRTHLVSPAVAAATAVRGKLSSPADLAKSVK
ECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure 
TSVQQPRTLAEKVWDSHVVVRGTGEGEAREPDLIYIDLHLVHEVTSPQAFDGLRLAGRQ
CCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCHHCCHHHHCHH
VRRPDLTIATEDHNVPTIDIDKPIADPVSRTQVETLRKNCEEFGIRLHPMGDAEQGIVHV
CCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCCEEEE
VGPQLGLTQPGTTVVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFRTMAINI
ECCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEE
DGSLPTGVTSKDVILAVIAKIGTGGGQGYVLEYRGQAVEEMSMEARMTMCNMSIEAGARA
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHCCCCC
GMVAPDETTYAYLKDRPHAPKGQLWDAAVAEWDSLRTDEGAQFDAEVHIDASTLTPFVTW
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCEEEEE
GTNPGQGVPLGADVPDPELMADEEERLAAEKALTYMDLTPGTPMREIPVDTVFVGSCTNG
CCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHEEEECCCCCCHHHCCCCEEEEECCCCC
RIEDLRAVADVLRDRKVAAGVTMLIVPGSMRVRAQAESEGLGEIFAAAGAQWRQAGCSMC
HHHHHHHHHHHHHCCHHHCCEEEEEECCCEEEEEECCCCCHHHHHHHCCHHHHHHCCCEE
LGMNPDQLSPGQRCASTSNRNFEGRQGKGGRTHLVSPAVAAATAVRGKLSSPADLAKSVK
ECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA