Click here to switch to the map view.

The map label for this gene is glnB [H]

Identifier: 169630320

GI number: 169630320

Start: 3275309

End: 3275647

Strand: Reverse

Name: glnB [H]

Synonym: MAB_3239c

Alternate gene names: 169630320

Gene position: 3275647-3275309 (Counterclockwise)

Preceding gene: 169630321

Following gene: 169630319

Centisome position: 64.64

GC content: 65.49

Gene sequence:

>339_bases
ATGAAGCTGGTCACCGCGATCGTCAAACCGTTCACACTCGAAGATGTCAAGACCGGCCTGGAGCAGGCAGGCATCCTCGG
CATGACGGTGAGCGAGGTGCAGGGCTACGGCCGGCAGAAGGGCCACACCGAGGTGTACCGCGGCGCCGAATACTCGGTGG
ACTTCGTGCCGAAAGTTCGTGTCGAGGTAGTGGTGGACGATAGCGCCGTCGACAAGGTGGTGGAGGTCATCGTGACCGCC
GCACGCACCGGCAAGATCGGCGACGGCAAGGTGTGGGTGAGCCCGGTGGACTCGGTGGTGCGGGTGCGCACCGGCGAGCG
GGGCGCCGATGCCCTCTAA

Upstream 100 bases:

>100_bases
CACGCTGAGGGTGCATACGACTTTGTCGCACTGGGAAGTGGTTCGGTCATCGGCCGCCACGTCAGCTCGGGCAGCTCGGA
CTAGGGAGAAAGAGGAAGAC

Downstream 100 bases:

>100_bases
CCGCTCAGTACGACAAACGGAACGGTAATCACATGGGCGCCGCAACGGACCTCGTGGCCGCCAGGGCGCAGCTGCTCGGC
GATAGCGGTCGGCTGAATGC

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLVTAIVKPFTLEDVKTGLEQAGILGMTVSEVQGYGRQKGHTEVYRGAEYSVDFVPKVRVEVVVDDSAVDKVVEVIVTA
ARTGKIGDGKVWVSPVDSVVRVRTGERGADAL

Sequences:

>Translated_112_residues
MKLVTAIVKPFTLEDVKTGLEQAGILGMTVSEVQGYGRQKGHTEVYRGAEYSVDFVPKVRVEVVVDDSAVDKVVEVIVTA
ARTGKIGDGKVWVSPVDSVVRVRTGERGADAL
>Mature_112_residues
MKLVTAIVKPFTLEDVKTGLEQAGILGMTVSEVQGYGRQKGHTEVYRGAEYSVDFVPKVRVEVVVDDSAVDKVVEVIVTA
ARTGKIGDGKVWVSPVDSVVRVRTGERGADAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=57.1428571428571, Blast_Score=141, Evalue=9e-36,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=58.9285714285714, Blast_Score=139, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12031; Mature: 12031

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVTAIVKPFTLEDVKTGLEQAGILGMTVSEVQGYGRQKGHTEVYRGAEYSVDFVPKVR
CCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCCCEECCCCEE
VEVVVDDSAVDKVVEVIVTAARTGKIGDGKVWVSPVDSVVRVRTGERGADAL
EEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHEECCCCCCCCCC
>Mature Secondary Structure
MKLVTAIVKPFTLEDVKTGLEQAGILGMTVSEVQGYGRQKGHTEVYRGAEYSVDFVPKVR
CCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCCCEECCCCEE
VEVVVDDSAVDKVVEVIVTAARTGKIGDGKVWVSPVDSVVRVRTGERGADAL
EEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]