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The map label for this gene is amt [H]

Identifier: 169630321

GI number: 169630321

Start: 3275664

End: 3277034

Strand: Reverse

Name: amt [H]

Synonym: MAB_3240c

Alternate gene names: 169630321

Gene position: 3277034-3275664 (Counterclockwise)

Preceding gene: 169630322

Following gene: 169630320

Centisome position: 64.67

GC content: 64.77

Gene sequence:

>1371_bases
ATGGGTGTCCCGAATACCGGGGATACCGCGTGGATGCTGGCCAGCGCCGCGCTGGTGTTATTGATGACACCCGGTCTGGC
CTTCTTCTACGGCGGCATGGTCCGCGCGAAGAACGTGCTCAACATGATCATGATGAGTATCAGCGCCATGGGCCTGGTGA
CGGTGCTGTGGGTGCTGTACGGGTACTCGATGGCCTTCGGGAACGACGTCTCGGGCCTGTTCGGCAACCCGGCGCAGTTC
CTCGGACTCAAGGGACTCATCGGTGGCAACGCCGCCGAGGCTGTGGTGGCCGACCCGGCCAACGGCGTCGAGGCCGCCGA
GGTGGTCAACATCCCCTTGGTCGGGACTCTCCCGGCCACGGTGTTCGTGGCGTTTCAGCTCATGTTCGCGATCATCACCG
TCGCCCTCATCTCCGGTGCGGTCGCCGACCGCCTGAAGTTTGGATCGTGGCTGCTGTTCGCGGGCTTGTGGGTCACCGTC
GTCTACTTCCCGGTGGCGCACTGGGTGTTCGCCTTCGATGGTGCCGCTGCGGAGAAGGGCGGCTGGATTGCCAACCAGCT
CAAGGCAATCGACTTCGCCGGTGGTACCGCGGTGCATATCAATGCCGGTACCGCGGGTCTGGTGTTGGCCTTGATCCTCG
GCAAGCGCAAGGGCTGGCCGGGCACGCCGATGCGGCCCCACAACCTGCCGTTCGTCATGCTCGGTGCGGGGCTGCTGTGG
TTCGGCTGGTACGGGTTCAACGCCGGATCGGCCACCAGCGCCAACGGATTGGCCGGTTCGACCTTTGTGACCACCACCGT
GGCCACCGCCGCCGCCATGCTCGGCTGGCTGCTCACCGAGCGGATTCGCGACGGGCACGCCACCTCGCTGGGTGCGGCAT
CGGGAATCGTCGCTGGTTTGGTGGCCATCACGCCGTCCTGTTCGTCGGTGAATGTGCTTGGCGCCCTTGCCATTGGTGCC
ATCGCTGGCATCCTGTGCGCATTGGCGGTGGGCCTGAAATACCGGTTGGGCTTCGACGACTCCCTGGACGTGGTCGGTGT
GCACCTGGTGGGCGGAATTGTCGGCACATTGCTCATCGGTTTGTTCGCCGCGCCCGAGACCGGAGCGGCAGTGGCGGGCC
TTTTCTACGGCGGCGGTCTAGAACAGTTGTGGCGTCAAGCGGTTGGTGCCGGGGCGGTTCTGCTCTACTCGGCGATAGGC
ACCGCTATCGTTGCTTTCATCGTGAAGTACACCATTGGCCTGCGCATCAGCGACGAGGGAGAGGCGGCTGGCGCCGACGA
ATCCCAACACGCTGAGGGTGCATACGACTTTGTCGCACTGGGAAGTGGTTCGGTCATCGGCCGCCACGTCAGCTCGGGCA
GCTCGGACTAG

Upstream 100 bases:

>100_bases
CACACGCGCGCCCTCTCGGAAACATCCGCTGCGCATTGTTCCTAGCGCAACCTGCTGAGATTTCCTGAGAGGAATGCCGC
TAGTGGATGGTTTCCCGTCA

Downstream 100 bases:

>100_bases
GGAGAAAGAGGAAGACATGAAGCTGGTCACCGCGATCGTCAAACCGTTCACACTCGAAGATGTCAAGACCGGCCTGGAGC
AGGCAGGCATCCTCGGCATG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MGVPNTGDTAWMLASAALVLLMTPGLAFFYGGMVRAKNVLNMIMMSISAMGLVTVLWVLYGYSMAFGNDVSGLFGNPAQF
LGLKGLIGGNAAEAVVADPANGVEAAEVVNIPLVGTLPATVFVAFQLMFAIITVALISGAVADRLKFGSWLLFAGLWVTV
VYFPVAHWVFAFDGAAAEKGGWIANQLKAIDFAGGTAVHINAGTAGLVLALILGKRKGWPGTPMRPHNLPFVMLGAGLLW
FGWYGFNAGSATSANGLAGSTFVTTTVATAAAMLGWLLTERIRDGHATSLGAASGIVAGLVAITPSCSSVNVLGALAIGA
IAGILCALAVGLKYRLGFDDSLDVVGVHLVGGIVGTLLIGLFAAPETGAAVAGLFYGGGLEQLWRQAVGAGAVLLYSAIG
TAIVAFIVKYTIGLRISDEGEAAGADESQHAEGAYDFVALGSGSVIGRHVSSGSSD

Sequences:

>Translated_456_residues
MGVPNTGDTAWMLASAALVLLMTPGLAFFYGGMVRAKNVLNMIMMSISAMGLVTVLWVLYGYSMAFGNDVSGLFGNPAQF
LGLKGLIGGNAAEAVVADPANGVEAAEVVNIPLVGTLPATVFVAFQLMFAIITVALISGAVADRLKFGSWLLFAGLWVTV
VYFPVAHWVFAFDGAAAEKGGWIANQLKAIDFAGGTAVHINAGTAGLVLALILGKRKGWPGTPMRPHNLPFVMLGAGLLW
FGWYGFNAGSATSANGLAGSTFVTTTVATAAAMLGWLLTERIRDGHATSLGAASGIVAGLVAITPSCSSVNVLGALAIGA
IAGILCALAVGLKYRLGFDDSLDVVGVHLVGGIVGTLLIGLFAAPETGAAVAGLFYGGGLEQLWRQAVGAGAVLLYSAIG
TAIVAFIVKYTIGLRISDEGEAAGADESQHAEGAYDFVALGSGSVIGRHVSSGSSD
>Mature_455_residues
GVPNTGDTAWMLASAALVLLMTPGLAFFYGGMVRAKNVLNMIMMSISAMGLVTVLWVLYGYSMAFGNDVSGLFGNPAQFL
GLKGLIGGNAAEAVVADPANGVEAAEVVNIPLVGTLPATVFVAFQLMFAIITVALISGAVADRLKFGSWLLFAGLWVTVV
YFPVAHWVFAFDGAAAEKGGWIANQLKAIDFAGGTAVHINAGTAGLVLALILGKRKGWPGTPMRPHNLPFVMLGAGLLWF
GWYGFNAGSATSANGLAGSTFVTTTVATAAAMLGWLLTERIRDGHATSLGAASGIVAGLVAITPSCSSVNVLGALAIGAI
AGILCALAVGLKYRLGFDDSLDVVGVHLVGGIVGTLLIGLFAAPETGAAVAGLFYGGGLEQLWRQAVGAGAVLLYSAIGT
AIVAFIVKYTIGLRISDEGEAAGADESQHAEGAYDFVALGSGSVIGRHVSSGSSD

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=439, Percent_Identity=42.5968109339408, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32564042, Length=440, Percent_Identity=27.9545454545455, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=471, Percent_Identity=26.7515923566879, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=445, Percent_Identity=26.0674157303371, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17550180, Length=467, Percent_Identity=26.9807280513919, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=445, Percent_Identity=34.1573033707865, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6321559, Length=457, Percent_Identity=32.1663019693654, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6324187, Length=446, Percent_Identity=33.6322869955157, Blast_Score=219, Evalue=9e-58,
Organism=Drosophila melanogaster, GI161078314, Length=440, Percent_Identity=30.2272727272727, Blast_Score=142, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46374; Mature: 46243

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVPNTGDTAWMLASAALVLLMTPGLAFFYGGMVRAKNVLNMIMMSISAMGLVTVLWVLY
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYSMAFGNDVSGLFGNPAQFLGLKGLIGGNAAEAVVADPANGVEAAEVVNIPLVGTLPAT
HHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHEEECCEECHHHHH
VFVAFQLMFAIITVALISGAVADRLKFGSWLLFAGLWVTVVYFPVAHWVFAFDGAAAEKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GWIANQLKAIDFAGGTAVHINAGTAGLVLALILGKRKGWPGTPMRPHNLPFVMLGAGLLW
CCHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
FGWYGFNAGSATSANGLAGSTFVTTTVATAAAMLGWLLTERIRDGHATSLGAASGIVAGL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAITPSCSSVNVLGALAIGAIAGILCALAVGLKYRLGFDDSLDVVGVHLVGGIVGTLLIG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
LFAAPETGAAVAGLFYGGGLEQLWRQAVGAGAVLLYSAIGTAIVAFIVKYTIGLRISDEG
HHCCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCC
EAAGADESQHAEGAYDFVALGSGSVIGRHVSSGSSD
CCCCCCCCCCCCCCEEEEEECCCCEEEHCCCCCCCC
>Mature Secondary Structure 
GVPNTGDTAWMLASAALVLLMTPGLAFFYGGMVRAKNVLNMIMMSISAMGLVTVLWVLY
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYSMAFGNDVSGLFGNPAQFLGLKGLIGGNAAEAVVADPANGVEAAEVVNIPLVGTLPAT
HHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHEEECCEECHHHHH
VFVAFQLMFAIITVALISGAVADRLKFGSWLLFAGLWVTVVYFPVAHWVFAFDGAAAEKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GWIANQLKAIDFAGGTAVHINAGTAGLVLALILGKRKGWPGTPMRPHNLPFVMLGAGLLW
CCHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
FGWYGFNAGSATSANGLAGSTFVTTTVATAAAMLGWLLTERIRDGHATSLGAASGIVAGL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAITPSCSSVNVLGALAIGAIAGILCALAVGLKYRLGFDDSLDVVGVHLVGGIVGTLLIG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
LFAAPETGAAVAGLFYGGGLEQLWRQAVGAGAVLLYSAIGTAIVAFIVKYTIGLRISDEG
HHCCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCC
EAAGADESQHAEGAYDFVALGSGSVIGRHVSSGSSD
CCCCCCCCCCCCCCEEEEEECCCCEEEHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]